Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F19 I0 R1 32 84.8 2851444 95.0% 2708871 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 2,795,396 C→A 100% Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,795,3960CA100.0% 276.4 / NA 83Q382K (CAG→AAG) gabPgamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/45);  total (38/45)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTA  >  NC_000913_3_pae_tpiA/2795252‑2795539
                                                                                                                                                |                                                                                                                                               
ccGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTGGTCATCGCCGTTTCAAAGtt                                                                                                                                             >  2:1328984/1‑149 (MQ=255)
  gTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGc                                                                                                                                           <  1:455022/149‑1 (MQ=255)
   tACGTGGCCGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCg                                                                                                                                          <  1:859377/149‑1 (MQ=255)
     cGTGGCGGTGTTACTCTCCACCGGCGCGCCACTCTAAACGGTGGTGGTGAACTATTACGCGCCTGCGAATGTAAGTAAATTTCTAATCGACATGTCCGGCGCTATCGCCCTGCTGGTTTATGTAGTCATCGCCGTTTCAAAGTTGCGGa                                                                                                                                        <  2:593439/149‑1 (MQ=255)
      gTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGAt                                                                                                                                       >  1:1204145/1‑149 (MQ=255)
         gCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATgcg                                                                                                                                    >  1:1246354/1‑149 (MQ=255)
             tgtTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCaaa                                                                                                                                >  1:82428/1‑149 (MQ=255)
                tctcttcCCCCTGCTCGGCATTCGTAACGGTCGTGGTGACCTATTACGCGCCTGCGAAGGTATTTACATTTCTGATCGACAGCACCGGCGCTATCGCCCTGCTGTTTTATTTAGTCATCGCCGCTTCAAAGTTGCGGATGCCCAAAAtt                                                                                                                             <  2:1182645/144‑1 (MQ=255)
                  ctctCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCt                                                                                                                           >  1:612569/1‑149 (MQ=255)
                   tctcCCCCGGCGCGGCATTCGTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGACGCGCAAAATTCTg                                                                                                                          <  2:534958/149‑1 (MQ=255)
                      cctccGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGCTGCGCAAAATTCTGCGa                                                                                                                       <  2:840493/146‑1 (MQ=255)
                        aCCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGc                                                                                                                     >  1:1302962/1‑149 (MQ=255)
                         ccGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCa                                                                                                                    <  1:821825/149‑1 (MQ=255)
                                    ttCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGa                                                                                                         >  1:738922/1‑149 (MQ=255)
                                    ttCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGa                                                                                                         >  1:306970/1‑149 (MQ=255)
                                      cTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGaaa                                                                                                       <  1:713429/149‑1 (MQ=255)
                                            ggtggtggtGAACTATTCCGCGCCTGCGAAGGTATTTAAATTTCTGATCTACAGCTCCTGCGCTATCGCCCTGTTGATTTATTTATTCAGCGCCGGTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCt                                                                                                 <  2:942833/149‑1 (MQ=255)
                                              tggtggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTg                                                                                               >  2:713744/1‑149 (MQ=255)
                                                 tggtgAACTACTACGCGCCTGCGAATGTATTTAACTTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCTCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAATTTCGCttcctt                                                                                            <  2:753777/149‑5 (MQ=255)
                                                  ggtgAACTATTACGCGCCTGCGAAGGTATTTAATTTTCTGATCGAAAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGaa                                                                                           <  1:903048/149‑1 (MQ=255)
                                                  ggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGaa                                                                                           >  1:1088569/1‑149 (MQ=255)
                                                    tgAACTATTCCCTCCCTCGGAAGTTATTTAATTTTCTGATCGACAGCGCCTGCGCAAGCGCCCTGCTGGTTTATTTATTCATCTCCGTTTCAAAGTTTCGTATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAtg                                                                                         <  2:1115708/149‑1 (MQ=255)
                                                     gAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAtgt                                                                                        >  1:1420355/1‑149 (MQ=255)
                                                      aaCTATTACGCGCCTGCGCAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGACGGAAGCGAAATTCGCTTGCGAAggtg                                                                                       >  1:249485/1‑149 (MQ=255)
                                                        cTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGc                                                                                     <  1:875669/149‑1 (MQ=255)
                                                        cTATTAAGCGCCTGCGAAGGTATTGTAATTTCTGATCGACAGCTCCAACGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGc                                                                                     <  2:862321/149‑1 (MQ=255)
                                                         tATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCt                                                                                    >  1:464045/1‑149 (MQ=255)
                                                         tATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCAGATGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCt                                                                                    >  1:1184601/1‑149 (MQ=255)
                                                            tACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTa                                                                                 <  1:1154835/149‑1 (MQ=255)
                                                               gcgcCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGTTTTATcc                                                                              <  2:884958/149‑1 (MQ=255)
                                                                 gcCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCGGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGt                                                                            >  1:271075/1‑149 (MQ=255)
                                                                   cTGGGAAGGTAGTTAATTTTCTGATCGACAGCGCCGGCGCCATCGCCCTGCCGGTTTATTTAGTCCTCGCCGTTTCAAAGTCGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTgg                                                                          <  1:1304031/149‑1 (MQ=255)
                                                                   cTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTgg                                                                          <  2:1422547/149‑1 (MQ=255)
                                                                   cTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTgg                                                                          <  1:739191/149‑1 (MQ=255)
                                                                    tGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGc                                                                         <  1:1420137/149‑1 (MQ=255)
                                                                    tGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGc                                                                         >  1:754005/1‑149 (MQ=255)
                                                                    tGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGc                                                                         <  2:456112/149‑1 (MQ=255)
                                                                       gAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAGGGAAGCGAAATTCGCTCGCGAAGGTGGCTTTATCCGTGGCTCa                                                                      >  2:477161/1‑149 (MQ=255)
                                                                        aaGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc                                                                     <  2:1170529/149‑1 (MQ=255)
                                                                        aaGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc                                                                     <  2:1204145/149‑1 (MQ=255)
                                                                        aaGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCCGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTGTGCGAGCAGAAGGAAGCGAAATTCGCTTTCGCATGTGGCTTTATCCGTGGCTCAc                                                                     <  1:819591/149‑1 (MQ=255)
                                                                            tATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTgg                                                                 <  2:442024/149‑1 (MQ=255)
                                                                              tttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTCGCTTTATCCGTGGCTCACCTGGCt                                                               >  1:362997/1‑149 (MQ=255)
                                                                               ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATTTTGCTTTATCCGTGGCTCACCTGGCTa                                                              <  2:526435/149‑1 (MQ=255)
                                                                                    tttCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCAt                                                         >  2:781295/1‑149 (MQ=255)
                                                                                        tGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc                                                     >  1:329923/1‑149 (MQ=255)
                                                                                           tCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTCGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCg                                                                                             <  1:972461/106‑1 (MQ=255)
                                                                                           tCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCg                                                                                             >  2:972461/1‑106 (MQ=255)
                                                                                           tCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCttt                                                  >  2:496946/1‑149 (MQ=255)
                                                                                           tCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCACCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCCGCttt                                                  >  2:1192326/1‑149 (MQ=255)
                                                                                            cGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTtta                                                 >  2:850473/1‑149 (MQ=255)
                                                                                                aGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAc                                             <  1:230413/149‑1 (MQ=255)
                                                                                                  cTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGATTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCt                                           >  2:1300395/1‑149 (MQ=255)
                                                                                                  cTCCGGCCTTATCGCCTTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCt                                           <  2:338211/149‑1 (MQ=255)
                                                                                                   tCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTAGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt                                          >  1:353966/1‑149 (MQ=255)
                                                                                                    ccGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCttt                                         <  1:723246/149‑1 (MQ=255)
                                                                                                      ggCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgt                                       >  2:832169/1‑149 (MQ=255)
                                                                                                          cTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTg                                   <  2:1370282/149‑1 (MQ=255)
                                                                                                           tATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATAGGCTTTATTACCTTTGTGTTgg                                  >  2:1424372/1‑149 (MQ=255)
                                                                                                            aTCGTCCTGCTGGGTTATTTAGTTATCGACGTTTCAAAGTTGCGGATGTGCAAAATTCTGCTAGCAGAAGGAAGCGAAATCCGCTTGCGAATGTGGCTTTATCCGTGGTTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGt                                 <  2:445701/149‑1 (MQ=255)
                                                                                                            aTCGCCCTGCTGGTTTATTTAGTCATCGTCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGt                                 <  2:445292/149‑1 (MQ=255)
                                                                                                             tCGCCCTGCTGCTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTa                                <  2:397788/149‑1 (MQ=255)
                                                                                                              cGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAg                               <  2:852409/149‑1 (MQ=255)
                                                                                                               gCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGATTACGAATGAGGCTTTAACCATTGCTCAACTGGCAAGTCATCGGCTTTATTACCTTTGTGTTGGTAGt                              >  1:190745/1‑149 (MQ=255)
                                                                                                                cccTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTg                             >  2:595136/1‑149 (MQ=255)
                                                                                                                 ccTGATGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGa                            <  1:108111/149‑1 (MQ=255)
                                                                                                                   tgcttGTTGATTTAGTTGTCGCCTCTTCAAAGTTGCGGATGCACAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTTCGAATGTGGCTTTATCCGTGGCTCACCTGTCTAGTCATCGTCTTTATTACCTTTGTGTTGGTAGTGATg                          <  2:653697/149‑1 (MQ=255)
                                                                                                                   tgctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGTCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATg                          >  1:829896/1‑149 (MQ=255)
                                                                                                                   tgctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCAGCTTTATTACCTTTGTGTTGGTAGTGATg                          >  2:1086316/1‑149 (MQ=255)
                                                                                                                     cgagTTGATTTAGTCAGCGCGTTGTCAAAGTTGCGTATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGGTGCTTACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCt                        <  2:828973/146‑1 (MQ=255)
                                                                                                                      ttgTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATACTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTa                       <  1:1331659/147‑1 (MQ=255)
                                                                                                                      tgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTa                       >  2:5290/1‑149 (MQ=255)
                                                                                                                         tttatttaGTCATCGGCGTTTCAAAGGTGCGGATGCGCAAAATTCTGCGAGCAGGAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtc                    <  1:908166/149‑1 (MQ=255)
                                                                                                                         tttatttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTTTGTTGGTAGTGCTGCTATtc                    >  2:1132417/1‑149 (MQ=255)
                                                                                                                          ttatttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtcc                   <  2:1166363/149‑1 (MQ=255)
                                                                                                                              ttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtcc               <  1:527636/149‑1 (MQ=255)
                                                                                                                              ttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGGAGTGATGCTATtccgtcc               >  1:750354/1‑149 (MQ=255)
                                                                                                                                aGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCgg             <  2:523708/149‑1 (MQ=255)
                                                                                                                                 gTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGgc            <  1:175274/149‑1 (MQ=255)
                                                                                                                                           tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAGGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGAGGCTATTCCGTCCGGCGCAACAGTTa  >  1:943119/1‑149 (MQ=255)
                                                                                                                                           tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGACGACCACATTAACCAATAGAAAACGGTGCTGTAGTCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTa  >  2:1003586/1‑149 (MQ=255)
                                                                                                                                           tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCGGGGGCAACAGTTa  >  2:455986/1‑149 (MQ=255)
                                                                                                                                           tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTa  <  1:850620/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTA  >  NC_000913_3_pae_tpiA/2795252‑2795539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A