Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F8 I1 R1 16 40.3 1420662 94.4% 1341104 140.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,795,396 C→A Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,795,3960CA100.0% 180.2 / NA 53Q382K (CAG→AAG) gabPgamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/24);  total (29/24)

CCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAG  >  NC_000913_3_pae_tpiA/2795251‑2795540
                                                                                                                                                 |                                                                                                                                                 
cccGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGt                                                                                                                                                >  2:582792/1‑149 (MQ=255)
  cGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTg                                                                                                                                              >  2:452663/1‑149 (MQ=255)
   gTACGTGGCGGTGTTACTCTCCACAGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACCCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCTGGCGGTATCGCGCTGTTGGTTTATTTGGTCTTCTGGGTTTGAAAGTTgg                                                                                                                                             >  1:105183/1‑148 (MQ=255)
            ggTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACAATTCAGCTCTTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTCTTTGGTCATCGCCGTTTCAAACTTGGGGaacggca                                                                                                                                    >  1:140329/1‑143 (MQ=255)
                    tctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTg                                                                                                                            <  1:397559/149‑1 (MQ=255)
                    tctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTg                                                                                                                            <  2:401664/149‑1 (MQ=255)
                       ccACCGGCGCGGCATTCTTAACGGGGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTCGCGGATGCGCAAAATTCTGCGa                                                                                                                         <  2:617693/149‑1 (MQ=255)
                           cGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAg                                                                                                                     >  2:683769/1‑149 (MQ=255)
                            ggCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAga                                                                                                                    <  1:512621/149‑1 (MQ=255)
                               gcgGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGAATGCGCAAAATTCTGCGAGCAga‑agg                                                                                                                >  1:186156/1‑149 (MQ=255)
                                 ggCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAga‑aggaa                                                                                                              >  1:104689/1‑149 (MQ=255)
                                 ggCATGCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAga‑aggaa                                                                                                              <  2:195732/149‑1 (MQ=255)
                                  gCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAga‑aggaag                                                                                                             >  1:297058/1‑149 (MQ=255)
                                            tggtggtggtGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGc                                                                                                   <  1:708228/148‑1 (MQ=255)
                                                 gtggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGGGCGGA‑CGGAAGCGAAATTCGCTTGCg                                                                                              >  1:152743/1‑149 (MQ=255)
                                                    gtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGc                                                                                                                                             >  2:535201/1‑100 (MQ=255)
                                                    gtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGc                                                                                                                                             <  1:535201/100‑1 (MQ=255)
                                                          tATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCt                                                                                     <  1:84054/149‑1 (MQ=255)
                                                           aTTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCtt                                                                                    >  1:706440/1‑149 (MQ=255)
                                                           aTTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATCCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCtt                                                                                    <  1:670847/149‑1 (MQ=255)
                                                                  gcCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGt                                                                             >  1:214216/1‑149 (MQ=255)
                                                                   ccTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTg                                                                            <  1:452663/149‑1 (MQ=255)
                                                                    cTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTgg                                                                           <  2:297058/149‑1 (MQ=255)
                                                                               tttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCa                                                                                                                      >  1:344190/1‑96 (MQ=255)
                                                                               tttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCa                                                                                                                      <  2:344190/96‑1 (MQ=255)
                                                                                ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAga‑aggaag                                                                                                             <  2:581685/103‑1 (MQ=255)
                                                                                ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAga‑aggaag                                                                                                             >  1:581685/1‑103 (MQ=255)
                                                                                ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCt                                                                    <  1:543324/144‑1 (MQ=255)
                                                                                ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCt                                                                    >  2:543324/1‑144 (MQ=255)
                                                                                ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa                                                               >  1:106173/1‑149 (MQ=255)
                                                                                 tAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTTGCTTGCGAATGTGGCTTTATCCGTGGCTAACCTGGCTAg                                                              >  1:432401/1‑149 (MQ=255)
                                                                                       tCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCg                                                        >  2:393651/1‑149 (MQ=255)
                                                                                           aTCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt                                                    <  1:110532/149‑1 (MQ=255)
                                                                                             cGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTtta                                                  <  2:104689/149‑1 (MQ=255)
                                                                                              gACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCGttat                                                 >  2:692917/1‑149 (MQ=255)
                                                                                               aCAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCACAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGATTGCGAATGTAGCTTTATCCGTGGCACACCTGGCTCGCCATCGGCGttatt                                                >  2:20806/1‑149 (MQ=255)
                                                                                                  gCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAcc                                             <  1:582792/149‑1 (MQ=255)
                                                                                                           cTATCGCCCTGCTGGTTTATTTAGTCATCGCCGGTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTg                                    <  1:22535/149‑1 (MQ=255)
                                                                                                            tATCGCGCTGATGGTTTATTTAGTCATCGCCGTTTCAAAGTGGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTTACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg                                   <  2:279452/149‑1 (MQ=255)
                                                                                                                  ccTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGc                                                                 <  2:315619/113‑1 (MQ=255)
                                                                                                                  ccTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGc                                                                 >  1:315619/1‑113 (MQ=255)
                                                                                                                         gTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTAtt                      <  2:106173/149‑1 (MQ=255)
                                                                                                                         gTTTATTTAGGCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTATGC‑AGAAGACGGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTAtt                      <  1:20806/149‑1 (MQ=255)
                                                                                                                              tttaGTCATCGCCGTTTCAAAGTTGCGAATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAACTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtc                 >  2:65917/1‑149 (MQ=255)
                                                                                                                               ttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtcc                >  1:650244/1‑149 (MQ=255)
                                                                                                                                 aGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCgg              >  1:391544/1‑149 (MQ=255)
                                                                                                                                  gTCATCGCCGTTTCAAAGTTGCGGAGGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCATCCGgc             >  1:644733/1‑149 (MQ=255)
                                                                                                                                     aTCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTTCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGAGCa          >  1:425726/1‑149 (MQ=255)
                                                                                                                                     aTCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCa          >  1:343587/1‑149 (MQ=255)
                                                                                                                                            tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCt                                                     >  1:119456/1‑99 (MQ=255)
                                                                                                                                            tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCCCAACCGTTa   >  1:684973/1‑149 (MQ=255)
                                                                                                                                            tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCGTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCt                                                     <  2:119456/99‑1 (MQ=255)
                                                                                                                                             ttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAg  <  1:221319/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGA‑AGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAG  >  NC_000913_3_pae_tpiA/2795251‑2795540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A