Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F64 I1 R1	18	58.3	2092080	92.1%	1926805	145.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,795,396	C→A	Q382K (CAG→AAG)	gabP →	gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,795,396	0	C	A	98.2%	161.3 / NA	54	Q382K (CAG→AAG)	gabP	gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (26/27); total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTA  >  NC_000913_3_pae_tpiA/2795252‑2795539

ccGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGtt                                                                                                                                             >  2:746375/1‑149 (MQ=255)

 cGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTg                                                                                                                                            >  1:772760/1‑149 (MQ=255)

   tACGTGGCGGTGTTACTCTCCACCGGCGCGGCACTCTGATCTGTGGTTGTGAACTAGTGTGCGCCTGCGCAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCTCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCg                                                                                                                                          <  2:521239/149‑1 (MQ=255)

   tACCTGGCCGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGAACGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCg                                                                                                                                          <  1:368458/149‑1 (MQ=255)

              gtTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCCCCGTTTCAAAGTTGCGGATGCGCaaaa                                                                                                                               >  1:76979/1‑149 (MQ=255)

                   tctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTg                                                                                                                          >  1:270586/1‑149 (MQ=255)

                    ctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGc                                                                                                                         <  1:935645/149‑1 (MQ=255)

                            gcgcGGCATTCTGAACGGTGGTGGTGAACTATTACGCGCCTGCGTAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgaa                                                                                                                 <  1:789844/149‑1 (MQ=255)

                                ggCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAATCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgatgaaa                                                                                                             >  1:449860/1‑144 (MQ=255)

                                ggCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGCCGCTATCGCCCTGCTGGTTTATTTAGTAATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGCGCAgaaggaa                                                                                                             >  1:549520/1‑149 (MQ=255)

                                 gCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTATGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTATCAAAGTTCCGCCGGCTCACAATTCAGCAGGAAgaaggaaa                                                                                                            >  2:195170/1‑148 (MQ=255)

                                         aaCGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACACCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTTCGGATGCCCAAAATTCTGCGAGCAGAAGGAAGCGAAATTc                                                                                                    >  2:294554/1‑149 (MQ=255)

                                          aCGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCATTTCAAAGTTGCGGATGCGCCAAATTCTGCGAGCAGAAGGAAGCGAAATTCg                                                                                                   >  2:130544/1‑149 (MQ=255)

                                           cGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCAGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCACGCAGAAGGAAGCGAAATTCGc                                                                                                  >  1:498487/1‑149 (MQ=255)

                                            ggtggtggtGAACTATTACGCGCCTGCGAAGGTATTTAAAGTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCt                                                                                                 >  2:1008655/1‑149 (MQ=255)

                                              tggtggtgAACTGTTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGAt                                                                                                                                       >  2:646660/1‑109 (MQ=255)

                                              tggtggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGAt                                                                                                                                       <  1:646660/109‑1 (MQ=255)

                                                     gAACTATTACGCTCCTGCGAAGGTATTTAAATTTCTGATCTACAGCTCCGGCGCTATCGCCCTTCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAtgt                                                                                        <  1:790205/149‑1 (MQ=255)

                                                      aaCGATTACTCGCCTGCTAAGGTATTTAAATTTCTGATCGCCAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAtgtg                                                                                       <  2:141372/149‑1 (MQ=255)

                                                           ttACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCttt                                                                                  >  1:526671/1‑149 (MQ=255)

                                                                   cTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGaa                                                                                           <  2:437697/132‑1 (MQ=255)

                                                                   cTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGaa                                                                                           >  1:437697/1‑132 (MQ=255)

                                                                   cTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTgg                                                                          >  1:869723/1‑149 (MQ=255)

                                                                        aaGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGACGCACAATTAGCTTGAGAATGTCGCTTTATCCGTGGCTccc                                                                     >  2:821315/1‑147 (MQ=255)

                                                                             aTTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATTCGCAAAATTCTGTGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGc                                                                <  1:555677/149‑1 (MQ=255)

                                                                              tttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCt                                                               >  1:1031447/1‑149 (MQ=255)

                                                                               ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAATTTCGCGTGCGACTGTGGCTTTATCCGTGGCTCACCTGGCTa                                                              <  2:270586/149‑1 (MQ=255)

                                                                               ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa                                                              <  2:1024540/149‑1 (MQ=255)

                                                                               tcaaaTTTCTGATATACACCTCAGGCGCTATGGTCCTGCGCTTTGATTTAGTCAGCGCCTTTTCAAAGTGGCGGATGCGCAAAACTCTGCGAGCAGAAGGAAGCGAAAGTCGCTTGCGAATGTGGCTTTACCCGTGGCTCACCTGGCTa                                                              <  2:22766/147‑1 (MQ=255)

                                                                                      tctcaacGACAGCTCCGGCGCCATCGCCCTGCTGGTTTATTTAGTCATCGCCGTATCAAAGTTGCGGATGCGCAAAATTCTGCCAGCAGAAGGAATCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCg                                                       <  2:790069/143‑1 (MQ=255)

                                                                                         gATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCAGTTTCAAAGTTACGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCCCACATGGCTACTCATCGCCt                                                    >  2:197648/1‑149 (MQ=255)

                                                                                            cGACAGTCCCGGCGCTATCGCCCTGCGGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAGTCGCGTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTtta                                                 <  2:48729/149‑1 (MQ=255)

                                                                                              aCAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGc                                                                                                  <  2:378894/98‑1 (MQ=255)

                                                                                              aCAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGc                                                                                                  >  1:378894/1‑98 (MQ=255)

                                                                                              aCAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAATCATCGGCTttatt                                               >  1:936807/1‑149 (MQ=255)

                                                                                               cAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttatta                                              <  1:1008655/149‑1 (MQ=255)

                                                                                                    ccGGCGCTATCGGCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCttt                                         <  2:542856/149‑1 (MQ=255)

                                                                                                       gcgcTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGATTTATCCGTGGCTCACCTGGCTAGTCCTCGGCTTTATTAACTTtgtg                                      >  1:65382/1‑149 (MQ=255)

                                                                                                          ctactgtccTGCTTGTTTATTTAGTCATCGTCGTTTCAAAGTTGCGCATGCGCAAAATTCTGCGAGCAGAAGGAAGACCACTTCACTATCGCCTGGTTGGGCATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTg                                   <  2:119799/142‑1 (MQ=255)

                                                                                                               gCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACATTTGAGCTGCTAGt                              >  1:710178/1‑149 (MQ=255)

                                                                                                                 ccTGCTTGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGa                            <  1:134027/149‑1 (MQ=255)

                                                                                                                  ctgctgGTTTATTTAGTCATCGCCGGTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGAt                           <  2:96445/149‑1 (MQ=255)

                                                                                                                   tgctgGTTTATTTAGTCATCGACGTTTAACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGAGAAATTCGCTTGCGCATATCGCTCACTCCGTTGCTCACCTGCCTAGTCAACGGCTTTATACCCTTGGTGTTGGCAGtagag                          >  2:425214/1‑145 (MQ=255)

                                                                                                                             attatgcATCGCTGTTTCAAAGTGGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtc                <  2:76979/143‑1 (MQ=255)

                                                                                                                              ttaGTCATCGCCTTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtcc               <  1:856580/149‑1 (MQ=255)

                                                                                                                                aGTCATCGCCGTTTCAAAGTTGCCGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCgg             <  1:625411/149‑1 (MQ=255)

                                                                                                                                 gTCATCGCCTTTTCAAAGTAGCTGATGCGCAAAATTCTGCGAGCCGGAGTTATCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCGGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGgc            <  1:140425/149‑1 (MQ=255)

                                                                                                                                 gTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGgc            <  1:128162/149‑1 (MQ=255)

                                                                                                                                   cATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGgcgc          <  1:800327/149‑1 (MQ=255)

                                                                                                                                     tCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCaa        >  2:466859/1‑149 (MQ=255)

                                                                                                                                        ccGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAg     >  1:347555/1‑149 (MQ=255)

                                                                                                                                        ccGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGATGGTAGTCATGCTATTCCGTCCGGCGCAACAg     >  2:75428/1‑149 (MQ=255)

                                                                                                                                         cGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACATTTGTGTTGGTAGTGATGCTATTCCGGCCGGCGCAACAGt    <  2:324614/149‑1 (MQ=255)

                                                                                                                                           tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCACACGTCAGCGAAATTCGCTTGCGAATGTGCCTTTATCCGTCGCTCACCTGGCTAGTCATCGGCCTCATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGGCACCGTTa  >  2:270324/1‑149 (MQ=255)

CCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTA  >  NC_000913_3_pae_tpiA/2795252‑2795539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A