Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	170	48.1	1621790	99.2%	1608815	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,974,125	T→C	E630E (GAA→GAG)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,974,125	0	T	C	100.0%	161.9 / NA	46	E630E (GAA→GAG)	aas	acyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/20); total (26/20)

TTGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGC  >  NC_000913/2974002‑2974258

ttGCAACTTATCGCGCGTCAGTTCGTTATCTGTGATGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAATGCCAGTTGCTCCACCATTTCCAGCg                                                                                                                       >  1:256393/1‑140 (MQ=255)

 tGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGa                                                                                                                      <  2:371645/140‑1 (MQ=255)

  gCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAACAACCAAGTGCCAGTTGCTCCACCATTTCCAGCgcc                                                                                                                     >  2:500235/1‑138 (MQ=255)

  gCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGac                                                                                                                     >  2:454428/1‑140 (MQ=255)

     aCTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACAcc                                                                                                                  >  2:678264/1‑140 (MQ=255)

         aTCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCAttt                                                                                                              >  2:802322/1‑140 (MQ=255)

          tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTc                                                                                                             >  2:513861/1‑140 (MQ=255)

            gcgcgTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGc                                                                                                           <  2:454496/140‑1 (MQ=255)

                 tCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCa                                                                                                      >  1:46390/1‑140 (MQ=255)

                          tATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCa                                                                                             >  2:339540/1‑140 (MQ=255)

                                tggtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGt                                                                                       >  2:660918/1‑140 (MQ=255)

                                  gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGttt                                                                                     >  2:343641/1‑140 (MQ=255)

                                      aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTgcg                                                                                 <  2:578883/140‑1 (MQ=255)

                                      aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTgcg                                                                                 <  1:529692/140‑1 (MQ=255)

                                      aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTgcg                                                                                 >  2:603901/1‑140 (MQ=255)

                                      aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTgcg                                                                                 >  2:63095/1‑140 (MQ=255)

                                      aaaGCACCAGTGCCTCGCCTTTGCTGGCATCCCTCTTAATCGCAGTTGCATTGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCNTGNAATTTTNGCAAAGCGTTTTgcg                                                                                 <  1:500235/140‑1 (MQ=255)

                                            ccAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGccc                                                                           <  1:752499/140‑1 (MQ=255)

                                             cAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCt                                                                          >  1:166263/1‑140 (MQ=255)

                                             cAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCt                                                                          >  1:693294/1‑140 (MQ=255)

                                               gTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGa                                                                        >  2:86672/1‑140 (MQ=255)

                                               gTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGa                                                                        >  2:172431/1‑140 (MQ=255)

                                                 gCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAAt                                                                      <  1:172431/140‑1 (MQ=255)

                                                    tCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTg                                                                   >  1:654908/1‑140 (MQ=255)

                                                      gCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGca                                                                 <  1:660918/140‑1 (MQ=255)

                                                            gCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGc                                                           >  2:584553/1‑140 (MQ=255)

                                                                    cGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCg                                                   >  2:161400/1‑140 (MQ=255)

                                                                            aTCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACg                                           <  1:63095/140‑1 (MQ=255)

                                                                              cGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGca                                         <  1:143513/140‑1 (MQ=255)

                                                                                 aGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACaa                                      >  1:285977/1‑140 (MQ=255)

                                                                                   tGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAata                                    >  2:418545/1‑140 (MQ=255)

                                                                                   tGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAata                                    >  2:791106/1‑140 (MQ=255)

                                                                                     gCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATc                                  >  1:18657/1‑140 (MQ=255)

                                                                                      cATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAG‑‑CTGCTCGTCAAAACGCACAATATCGcc                               <  1:678264/140‑1 (MQ=255)

                                                                                       aTGGACTTTATCTGGCGAAACCCCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGc                                <  2:738056/140‑1 (MQ=255)

                                                                                       aTGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGc                                <  2:166263/140‑1 (MQ=255)

                                                                                       aTGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGc                                <  2:693294/140‑1 (MQ=255)

                                                                                       aTGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGc                                <  2:328554/140‑1 (MQ=255)

                                                                                           aCTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAgt                            <  1:339540/140‑1 (MQ=255)

                                                                                               tATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCa                        >  2:616379/1‑140 (MQ=255)

                                                                                               tATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCa                        >  1:18569/1‑140 (MQ=255)

                                                                                                     gCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTTGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAg                  >  2:28282/1‑140 (MQ=255)

                                                                                                     gCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAg                  <  1:529825/140‑1 (MQ=255)

                                                                                                        aaaCACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCcg               <  1:513861/140‑1 (MQ=255)

                                                                                                          acacCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCcgcg             <  2:285977/140‑1 (MQ=255)

                                                                                                                    ccAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcg   >  2:350816/1‑140 (MQ=255)

                                                                                                                     cAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgc  <  1:343641/140‑1 (MQ=255)

                                                                                                                       gttgctCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGcc                <  2:63078/124‑1 (MQ=255)

                                                                                                                       gttgctCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGcc                >  1:63078/1‑124 (MQ=255)

TTGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGC  >  NC_000913/2974002‑2974258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A