Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9101112 F55 I0 R1 156 414.6 11700970 99.6% 11654166 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,653,890 T→C 11.0% L245P (CTA→CCA)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,653,8900TC11.0% 996.5 / 112.9 364L245P (CTA→CCA) U712_08495GTP‑sensing transcriptional pleiotropic repressor CodY
Reads supporting (aligned to +/- strand):  ref base T (146/178);  new base C (19/21);  total (165/199)
Fisher's exact test for biased strand distribution p-value = 8.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

Only 100 of 369 total aligned reads displayed.

CGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATGTAATAATTTTAA  >  CP006881/1653741‑1654039
                                                                                                                                                     |                                                                                                                                                     
cGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACt                                                                                                                                                       <  2:1739351/150‑1 (MQ=255)
   aaaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCGTTACCCGTTCTGTTATTGTGAACGTACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCaaa                                                                                                                                                    <  1:3759947/150‑1 (MQ=255)
   aaaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCCGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGAAGAGCGCCGGTGTTAACGAGTCTAGATCATTAGAAATGAAACGGACTTATATCAAGGTACTaaa                                                                                                                                                    >  2:4519970/1‑150 (MQ=255)
      tGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGGGCCGGTGTTATGGAGTCTAGATCATTAGGAATGAAAGGTACTTATATGAAGATACCAaacaa                                                                                                                                                 >  1:2628294/1‑150 (MQ=255)
      tGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAaacaa                                                                                                                                                 <  2:4746194/150‑1 (MQ=255)
        aaGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGGAAGGTACTTATATCAAGGTACTAAacaaca                                                                                                                                               >  1:1127697/1‑150 (MQ=255)
        aaGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAacaaca                                                                                                                                               >  2:5131164/1‑150 (MQ=255)
            tcttctTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAAtt                                                                                                                                           <  1:1216252/150‑1 (MQ=255)
            tcttctTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAAtt                                                                                                                                           <  2:4116694/150‑1 (MQ=255)
            tcttctTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAAtt                                                                                                                                           <  2:2095354/150‑1 (MQ=255)
                 ttgttgCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTaa                                                                                                                                      <  1:1872873/150‑1 (MQ=255)
                   gttgCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAAtt                                                                                                                                    <  2:826627/150‑1 (MQ=255)
                     tgCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGa                                                                                                                                  >  2:4888532/1‑150 (MQ=255)
                      gCAAGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGaa                                                                                                                                 >  2:281925/1‑150 (MQ=255)
                        aaGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAAtt                                                                                                                               <  1:1027080/150‑1 (MQ=255)
                        aaGTAAAATTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAAtt                                                                                                                               <  1:2765175/150‑1 (MQ=255)
                         aGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAATTa                                                                                                                              >  2:539255/1‑150 (MQ=255)
                         aGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTACGAATAAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTa                                                                                                                              >  2:3662420/1‑150 (MQ=255)
                          gTAAGATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATAAATGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAg                                                                                                                             <  1:3321137/150‑1 (MQ=255)
                          atAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATAACAAGGTACTAAACAACAAATTCCTAATTGAATTaa                                                                                                                             >  1:1565446/2‑149 (MQ=255)
                               aTTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaat                                                                                                                        >  1:3559229/1‑150 (MQ=255)
                               aTTGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaat                                                                                                                        >  2:1240797/1‑150 (MQ=255)
                                 tGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatct                                                                                                                      >  2:4770051/1‑150 (MQ=255)
                                 tGCTGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatct                                                                                                                      <  1:917279/150‑1 (MQ=255)
                                   cTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatctaa                                                                                                                    <  1:678876/150‑1 (MQ=255)
                                    tGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATAAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatctaaa                                                                                                                   <  1:2244228/150‑1 (MQ=255)
                                    tGACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatctaaa                                                                                                                   >  1:4415490/1‑150 (MQ=255)
                                     gACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAATTAGaaaatctaaaa                                                                                                                  >  2:1623956/1‑150 (MQ=255)
                                     gACCGTGTCGACATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatctaaaa                                                                                                                  >  2:4204284/1‑150 (MQ=255)
                                        cGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatctaaaatct                                                                                                               <  2:5513699/150‑1 (MQ=255)
                                         gtgtCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAAtctc                                                                                                              <  2:1690286/150‑1 (MQ=255)
                                         gtgtCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAAtctc                                                                                                              <  2:5010432/150‑1 (MQ=255)
                                         gtgtCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAAtctc                                                                                                              <  2:878110/150‑1 (MQ=255)
                                             cGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATAACAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTa                                                                                                          <  1:1338738/150‑1 (MQ=255)
                                              gacATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTaa                                                                                                         >  1:5181521/3‑150 (MQ=255)
                                                cATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATc                                                                                                       <  2:3379103/150‑1 (MQ=255)
                                                 aTTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATca                                                                                                      <  1:4749877/150‑1 (MQ=255)
                                                      ccGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACaaaa                                                                                                 >  1:4159625/1‑150 (MQ=255)
                                                       cGTTCTGTTATTGTGGACGCACTCAGAACGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAGTCACaaaaa                                                                                                >  2:5256895/1‑150 (MQ=255)
                                                       cGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACaaaaa                                                                                                <  2:5848028/150‑1 (MQ=255)
                                                         ttCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGa                                                                                              <  1:2457964/150‑1 (MQ=255)
                                                             gTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAAccc                                                                                          >  2:1129424/1‑150 (MQ=255)
                                                                aTTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCttt                                                                                       >  2:5187040/1‑150 (MQ=255)
                                                                aTTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCttt                                                                                       <  2:5065586/150‑1 (MQ=255)
                                                                 ttGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCtatt                                                                                      <  1:3112461/150‑1 (MQ=255)
                                                                 ttGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATAAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCtttt                                                                                      <  2:1082053/150‑1 (MQ=255)
                                                                  tgtgAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCttttt                                                                                     <  2:3163936/150‑1 (MQ=255)
                                                                   gtgAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTg                                                                                    <  1:4549026/150‑1 (MQ=255)
                                                                    tgAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGa                                                                                   <  1:5037176/150‑1 (MQ=255)
                                                                     gAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAg                                                                                  >  1:3470298/1‑150 (MQ=255)
                                                                     gAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAg                                                                                  <  1:1715090/150‑1 (MQ=255)
                                                                      aaCGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAgg                                                                                 >  1:2051335/1‑150 (MQ=255)
                                                                          cACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTc                                                                             <  2:4724576/150‑1 (MQ=255)
                                                                          cACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTc                                                                             >  2:5588908/1‑150 (MQ=255)
                                                                             tCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCttt                                                                          >  1:3658992/1‑150 (MQ=255)
                                                                              cAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCtttt                                                                         <  1:547067/150‑1 (MQ=255)
                                                                              cAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCtttt                                                                         <  1:3406520/150‑1 (MQ=255)
                                                                              cAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACNNATATCAAGGTACCAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCtttt                                                                         <  1:791852/150‑1 (MQ=255)
                                                                               aGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCttttt                                                                        <  2:1521453/150‑1 (MQ=255)
                                                                                gAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCtttttt                                                                       >  2:1041701/1‑150 (MQ=255)
                                                                                    gCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTAtt                                                                   <  1:49385/150‑1 (MQ=255)
                                                                                      tGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTc                                                                 <  1:2535416/150‑1 (MQ=255)
                                                                                      tGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATTTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTc                                                                 <  1:4680709/150‑1 (MQ=255)
                                                                                               ccGGTGTTATCGAGTCTAGATCATTAGGAATAAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAgg                                                        <  2:3514005/150‑1 (MQ=255)
                                                                                                  gtgtTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGaaa                                                     >  2:3596258/1‑150 (MQ=255)
                                                                                                  gtgtTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGaaa                                                     <  1:578011/150‑1 (MQ=255)
                                                                                                       aTCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCaac                                                >  2:752116/1‑149 (MQ=255)
                                                                                                       aTCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAAt                                                <  2:2277128/150‑1 (MQ=255)
                                                                                                       aTCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAAt                                                <  1:4770051/150‑1 (MQ=255)
                                                                                                        tCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACCAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAAACCTTTTTGAGGGTTCTTATTTTATTTCAAATAAAGGAAATGAATa                                               >  2:4635530/1‑150 (MQ=255)
                                                                                                           aGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGc                                            >  2:1416634/1‑150 (MQ=255)
                                                                                                             tCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCtt                                          >  1:1763122/1‑150 (MQ=255)
                                                                                                              cTAGATCATTAGTAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGAGATGTTTTTTTATTTCAAAAAAAAGAAATCAACAAGCttt                                         >  1:5359357/1‑150 (MQ=255)
                                                                                                               tAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTa                                        <  2:4950535/150‑1 (MQ=255)
                                                                                                                     aTTAGGAATGAAAGGTACTTATATCACGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAGGAACCCTTTTTGAGGGGTCTTGTTTTATTTCAAATAAAGGACATCAATAAGCTTTATTTCCt                                  >  2:67491/1‑150 (MQ=255)
                                                                                                                     aTTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCt                                  >  1:3550871/1‑150 (MQ=255)
                                                                                                                        aGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTggg                               >  2:3805700/1‑150 (MQ=255)
                                                                                                                          gAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGtt                             <  2:3909734/150‑1 (MQ=255)
                                                                                                                           aaTGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTg                            >  2:5777736/1‑150 (MQ=255)
                                                                                                                            aTGAAAGGTACTTATATCAAGGTACTAAACAACAAATTACTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGa                           >  2:1297205/1‑150 (MQ=255)
                                                                                                                             tGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGaa                          <  1:2297772/150‑1 (MQ=255)
                                                                                                                              gAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGaaa                         <  1:2158481/150‑1 (MQ=255)
                                                                                                                              aaaaGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGaaa                         >  1:1492667/2‑150 (MQ=255)
                                                                                                                              aaaaGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTAAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGaaa                         <  1:298551/149‑1 (MQ=255)
                                                                                                                               aaaGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGATCCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAg                        <  1:4204284/150‑1 (MQ=255)
                                                                                                                               aaaGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAg                        >  1:1455368/1‑150 (MQ=255)
                                                                                                                                aaGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGt                       >  2:1917802/1‑150 (MQ=255)
                                                                                                                                 aGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTc                      >  2:4311506/1‑150 (MQ=255)
                                                                                                                                    tACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCttt                   <  1:4012722/150‑1 (MQ=255)
                                                                                                                                    tACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCttt                   >  2:2673764/1‑150 (MQ=255)
                                                                                                                                     aCTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGCGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGCGTTGAAAGTCTTTc                  <  1:429302/150‑1 (MQ=255)
                                                                                                                                        tataTCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTAt               >  2:2417817/1‑150 (MQ=255)
                                                                                                                                        tataTCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTAt               <  2:5818818/150‑1 (MQ=255)
                                                                                                                                         atatCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATg              >  1:2848101/1‑150 (MQ=255)
                                                                                                                                          tatCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATGt             >  2:2558195/1‑150 (MQ=255)
                                                                                                                                            tCAAGGTACCAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATGtaa           >  1:1957739/1‑150 (MQ=255)
                                                                                                                                              aaGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTGTTTTTTTATTTCAAATAAAGAAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATGtaata         >  1:3205677/1‑150 (MQ=255)
                                                                                                                                              aaGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATGtaata         >  1:5633140/1‑150 (MQ=255)
                                                                                                                                               aGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATGtaataa        <  2:41771/150‑1 (MQ=255)
                                                                                                                                                   aCTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATGTAATAAtttt    >  1:3874970/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTGGGTTGAAAGTCTTTCTATGTAATAATTTTAA  >  CP006881/1653741‑1654039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A