breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA CP006881 30,182 C→T 100% intergenic (+147/‑96) U712_00120 → / → U712_20865 Sigma‑K factor‑processing regulatory protein BofA/16S ribosomal RNA
RA CP006881 52,751 G→T 20.1% intergenic (+199/‑12) U712_00220 → / → U712_00225 Sporulation‑specific protease yabG/Protein veg
RA CP006881 52,896 T→C 10.1% V45A (GTT→GCT)  U712_00225 → Protein veg
RA CP006881 125,619 C→T 6.7% R21C (CGT→TGT)  U712_00560 → DNA‑directed RNA polymerase subunit beta'
RA CP006881 310,878 G→T 6.2% intergenic (+31/+7) U712_01480 → / ← U712_01485 putative zinc transport system permease protein AdcB/Uncharacterized protein yceB
RA CP006881 430,099 C→G 6.8% intergenic (+22/+78) U712_01955 → / ← U712_01960 Phosphatase rapC inhibitor/Hypothetical Protein
RA CP006881 660,041 C→A 8.7% intergenic (+9/‑150) U712_03220 → / → U712_03225 Uncharacterized protein yebC/Uncharacterized protein yebD
RA CP006881 916,340 C→A 8.1% intergenic (+12/+67) U712_21235 → / ← U712_04455 tRNA‑Leu/Sporulation‑control protein spo0M
RA CP006881 924,066:1 +A 100% intergenic (‑46/‑233) U712_04490 ← / → U712_04495 Vegetative catalase/Aliphatic sulfonates import ATP‑binding protein SsuB
RA CP006881 1,345,152 A→G 5.3% P32P (CCA→CCG U712_06865 → Organic hydroperoxide resistance protein ohrB
RA CP006881 1,462,729 G→T 7.1% intergenic (+26/‑198) U712_07520 → / → U712_07525 Molybdopterin synthase sulfur carrier subunit/putative ABC transporter ATP‑binding protein YknU
RA CP006881 1,653,491 C→T 6.0% T112I (ACA→ATA)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,653,713 C→T 5.2% A186V (GCA→GTA)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,653,734 A→G 8.2% E193G (GAG→GGG)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,653,736 C→T 35.9% L194F (CTT→TTT)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,653,788 G→A 16.0% G211D (GGC→GAC)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,653,867 G→A 7.4% M237I (ATG→ATA U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,653,890 T→C 11.0% L245P (CTA→CCA)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY
RA CP006881 1,657,920 T→A 5.1% V255D (GTC→GAC)  U712_08520 → Flagellar motor switch protein FliG
RA CP006881 1,658,610 C→T 7.1% Q149* (CAA→TAA)  U712_08525 → putative flagellar assembly protein fliH
JC CP006881 1,662,121 (AGCTGAAAGCC)2→1 9.1% coding (806‑816/1464 nt) U712_08545 → putative flagellar hook‑length control protein
RA CP006881 1,669,467 C→T 13.0% R204* (CGA→TGA)  U712_08600 → Flagellar biosynthetic protein fliR
RA CP006881 1,671,433 G→C 12.9% D228H (GAT→CAT)  U712_08610 → Flagellar biosynthesis protein flhA
RA CP006881 1,862,707 T→C 34.8% A27A (GCT→GCC U712_09270 → Uncharacterized protein yncE
RA CP006881 2,052,333 C→A 7.4% intergenic (‑288/+69) U712_10170 ← / ← U712_10175 Uncharacterized protein yozO/Uncharacterized protein yozC
RA CP006881 2,054,382 A→C 100% intergenic (+60/‑41) U712_10180 → / → U712_10185 Putative aldehyde dehydrogenase dhaS/Squalene‑‑hopene cyclase
RA CP006881 2,235,729 C→T 5.3% E26K (GAA→AAA)  U712_11225 ← Transcriptional regulatory protein resD
RA CP006881 2,249,253 C→A 5.2% intergenic (‑42/+352) U712_11310 ← / ← U712_11315 Riboflavin biosynthesis protein RibD/Uncharacterized protein ypuD
RA CP006881 2,370,905 C→A 7.4% intergenic (+51/+42) U712_12015 → / ← U712_12020 HTH‑type transcriptional regulator sinR/Spore coat‑associated protein N
RA CP006881 2,801,996 G→A 100% A327T (GCC→ACC)  U712_14440 → UPF0118 membrane protein ytvI
RA CP006881 2,807,680 T→C 7.3% intergenic (‑261/+74) U712_14470 ← / ← U712_14475 Acetyl‑coenzyme A carboxylase carboxyl transferase subunit beta/putative NAD‑dependent malic enzyme 4
RA CP006881 2,807,683 G→C 12.7% intergenic (‑264/+71) U712_14470 ← / ← U712_14475 Acetyl‑coenzyme A carboxylase carboxyl transferase subunit beta/putative NAD‑dependent malic enzyme 4
RA CP006881 2,909,199 C→T 10.8% G49E (GGA→GAA)  U712_14990 ← Biotin synthase
RA CP006881 2,952,816 C→A 6.0% intergenic (+5/+22) U712_15205 → / ← U712_15210 putative ABC transporter permease protein ytlD/Putative 7,8‑dihydro‑8‑oxoguanine‑triphosphatase ytkD
RA CP006881 2,963,937 G→A 35.8% intergenic (‑124/+156) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
RA CP006881 2,963,941 A→G 11.0% intergenic (‑128/+152) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
RA CP006881 2,963,948 G→A 6.8% intergenic (‑135/+145) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
RA CP006881 3,153,598 C→A 7.8% intergenic (‑412/+7) U712_16190 ← / ← U712_16195 Hypothetical Protein/putative xanthine dehydrogenase subunit E
RA CP006881 3,350,137 C→A 7.1% P59P (CCC→CCA U712_17215 → Hypothetical Protein
RA CP006881 3,350,164 G→T 6.8% intergenic (+12/+15) U712_17215 → / ← U712_17220 Hypothetical Protein/Phenolic acid decarboxylase padC
RA CP006881 3,578,466 Δ1 bp 100% intergenic (‑59/‑76) U712_18365 ← / → U712_18370 Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH
RA CP006881 3,579,785 Δ1 bp 100% coding (73/132 nt) U712_18380 → Hypothetical Protein
RA CP006881 3,581,989 A→T 17.7% *258K (TAA→AAA)  U712_18400 ← HTH‑type transcriptional activator mta
RA CP006881 3,653,809 C→A 19.8% intergenic (‑209/‑104) U712_18820 ← / → U712_18825 putative beta‑barrel protein ywiB/Subtilosin‑A
RA CP006881 3,723,022 G→A 100% intergenic (‑136/‑165) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,045 T→C 100% intergenic (‑159/‑142) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,066:1 +A 86.2% intergenic (‑180/‑121) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,067 G→T 100% intergenic (‑181/‑120) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,091 A→T 100% intergenic (‑205/‑96) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,095 T→A 100% intergenic (‑209/‑92) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,097 T→G 100% intergenic (‑211/‑90) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,118 C→T 100% intergenic (‑232/‑69) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,121 T→A 100% intergenic (‑235/‑66) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,130 A→T 100% intergenic (‑244/‑57) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,162 C→T 100% intergenic (‑276/‑25) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,166 C→G 100% intergenic (‑280/‑21) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,183 A→G 100% intergenic (‑297/‑4) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,204 G→A 100% L6L (TTG→TTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,210 A→G 100% K8K (AAA→AAG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,222 C→T 100% Y12Y (TAC→TAT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,231 C→A 100% N15K (AAC→AAA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,236 T→A 100% L17Q (CTA→CAA)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,246 T→C 100% F20F (TTT→TTC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,248 T→C 100% V21A (GTT→GCT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,252 T→A 100% S22S (TCT→TCA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,273 2 bp→TC 100% coding (87‑88/771 nt) U712_19195 → putative transporter ywcJ
RA CP006881 3,723,276 A→G 100% R30R (AGA→CGG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,291 C→T 100% S35S (AGC→AGT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,294 G→A 100% M36I (ATG→ATA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,300 C→T 100% I38I (ATC→ATT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,303 A→T 100% G39G (GGA→GGT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,306 C→T 100% F40F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,336 T→C 100% S50S (AGT→AGC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,344 C→T 100% S53F (TCT→TTT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,351 G→T 100% A55A (GCG→GCT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,366 T→C 100% A60A (GCT→GCC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,387 A→T 100% T67T (ACA→ACT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,406 C→A 100% L74M (CTG→ATG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,438 T→C 100% T84T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,447 T→C 100% T87T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,459 T→C 100% T91T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,474 A→G 100% R96R (CGA→CGG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,483 2 bp→CA 100% coding (297‑298/771 nt) U712_19195 → putative transporter ywcJ
RA CP006881 3,723,492 A→C 100% R102R (CGA→CGC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,501 G→A 100% L105L (CTG→CTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,505 C→T 100% L107L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,534 G→A 100% L116L (TTG→TTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,549 A→G 100% L121L (CTA→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,558 T→C 100% I124I (ATT→ATC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,573 A→G 100% T129T (ACA→ACG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,576 G→A 100% G130G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,589 G→C 100% A135P (GCT→CCT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,616 C→T 100% L144L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,642 C→G 100% P152P (CCC→CCG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,652 C→T 100% L156L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,666 G→A 100% G160G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,717 T→C 100% L177L (CTT→CTC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,729 G→A 100% G181G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,738 C→T 100% L184L (CTC→CTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,771 C→T 100% F195F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,783 C→T 100% F199F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,822 A→G 100% S212S (TCA→TCG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,853 G→C 100% V223M (GTA→CTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,855 A→G 100% V223M (GTA→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,879 T→C 100% I231I (ATT→ATC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,888 A→G 100% T234T (ACA→ACG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,897 C→T 100% N237N (AAC→AAT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,912 G→A 100% M242I (ATG→ATA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,915 C→T 100% V243V (GTC→GTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,933 T→C 100% Y249Y (TAT→TAC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,939 G→A 100% T251T (ACG→ACA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,942 C→G 100% L252L (CTC→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,948 C→T 100% P254P (CCC→CCT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,952 A→C 100% K256Q (AAA→CAA)  U712_19195 → putative transporter ywcJ
RA CP006881 3,724,053 C→T 100% E258E (GAG→GAA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,078 C→T 100% S250N (AGT→AAT)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,092 T→C 100% A245A (GCA→GCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,101 A→C 100% L242L (CTT→CTG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,119 A→G 100% F236F (TTT→TTC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,194 A→G 100% S211S (AGT→AGC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,200 T→C 100% A209A (GCA→GCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,208 T→G 100% R207R (AGA→CGA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,274 C→T 100% E185K (GAA→AAA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,392 A→C 100% L145L (CTT→CTG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,446 G→A 100% Y127Y (TAC→TAT U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,467 T→C 100% K120K (AAA→AAG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,542 A→G 100% S95S (TCT→TCC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,587 C→T 100% G80G (GGG→GGA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,623 A→T 100% I68I (ATT→ATA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,659 A→G 100% F56F (TTT→TTC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,678 T→C 100% H50R (CAC→CGC)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,719 G→A 100% D36D (GAC→GAT U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,749 T→C 100% P26P (CCA→CCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,808 G→A 100% L7L (CTA→TTA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,831 C→T 100% intergenic (‑5/+35) U712_19200 ← / ← U712_19205 SacPA operon antiterminator/Uncharacterized protein ywcI
RA CP006881 3,724,849 A→T 100% intergenic (‑23/+17) U712_19200 ← / ← U712_19205 SacPA operon antiterminator/Uncharacterized protein ywcI
RA CP006881 3,724,902 A→C 100% A89A (GCT→GCG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,724,977 T→C 100% Q64Q (CAA→CAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,724,980 T→C 100% K63K (AAA→AAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,010 C→T 100% E53E (GAG→GAA U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,025 A→G 100% L48L (CTT→CTC U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,031 C→T 100% A46A (GCG→GCA U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,043 C→A 100% A42A (GCG→GCT U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,049 T→C 100% K40K (AAA→AAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,082 A→C 100% A29A (GCT→GCG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,205:1 +T 100% intergenic (‑37/‑492) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,725,237 A→G 100% intergenic (‑69/‑460) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,725,239 2 bp→AA 100% intergenic (‑71/‑457) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,801,973 G→T 7.7% intergenic (‑95/+12) U712_19600 ← / ← U712_19605 Putative polysaccharide deacetylase yxkH/Maltodextrin import ATP‑binding protein MsmX
RA CP006881 4,026,253 C→T 37.3% A117T (GCG→ACG)  U712_20800 ← Nucleoid occlusion protein
RA Exported 2,205 C→T 39.8% intergenic (–/‑773)  / → eryR –//vntifkey=4
RA Exported 2,206 C→T 7.0% intergenic (–/‑772)  / → eryR –//vntifkey=4
RA Exported 2,207 C→T 12.4% intergenic (–/‑771)  / → eryR –//vntifkey=4
JC Exported 2,655 Δ3 bp 100% intergenic (–/‑321)  / → eryR –//vntifkey=4
JC Exported 3,843 Δ5 bp 100% intergenic (+128/‑24) eryR → / → ampR /vntifkey=4//vntifkey=4
RA Exported 5,049 C→T 100% intergenic (+318/‑839) ampR → / → lacZalpha /vntifkey=4//vntifkey=4

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP006881 166420–171323 176275–171335 13–9856 226 [220] [223] 227 U712_21020–[U712_21045] U712_21020,U712_21025,U712_21030,U712_21035,U712_21040,[U712_21045]
* * ÷ CP006881 245128 246158 1031 227 [224] [222] 227 U712_01140–[U712_01145] U712_01140,[U712_01145]
* * ÷ CP006881 1067398 1068670 1273 225 [220] [224] 226 [U712_05245]–U712_05250 [U712_05245],U712_05250
* * ÷ CP006881 1148994 1150759 1766 231 [224] [219] 225 U712_05685–[U712_05690] U712_05685,[U712_05690]
* * ÷ CP006881 1502992 1504699 1708 225 [221] [223] 228 [U712_07735]–U712_07740 [U712_07735],U712_07740
* * ÷ CP006881 1562249 1566697 4449 226 [221] [222] 227 U712_08060 U712_08060
* * ÷ CP006881 2261205 2262113 909 229 [224] [221] 226 U712_11400–[U712_11405] U712_11400,[U712_11405]
* * ÷ CP006881 3725636 3728179 2544 226 [220] [224] 230 U712_19210–[U712_19215] U712_19210,[U712_19215]
* * ÷ CP006881 3757666 3759712 2047 226 [220] [224] 230 U712_19375 U712_19375

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported = 214015411 (0.940)2778 (0.170) 278/278 NT 15.3% intergenic (–/‑838) –/eryR –//vntifkey=4
?Exported 6389 = 15402 (0.940)intergenic (+178/–) lacZalpha/– /vntifkey=4/–