Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F56 I0 R1 218 83.1 3292392 95.3% 3137649 126.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,657,920 T→A 19.6% V255D (GTC→GAC)  U712_08520 → Flagellar motor switch protein FliG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,657,9200TA19.6% 143.6 / 31.8 76V255D (GTC→GAC) U712_08520Flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base A (7/8);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTG  >  CP006881/1657788‑1658057
                                                                                                                                    |                                                                                                                                         
tAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTcg                                                                                                                                     <  1:404182/139‑1 (MQ=255)
tAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTcg                                                                                                                                     <  8:199404/139‑1 (MQ=255)
  gACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTcgcg                                                                                                                                   <  8:80272/139‑1 (MQ=255)
     agagGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTTGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATg                                                                                                                                >  5:359705/1‑139 (MQ=255)
       agGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGtt                                                                                                                              <  5:347608/139‑1 (MQ=255)
       agGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGtt                                                                                                                              >  2:381237/1‑139 (MQ=255)
        gggACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGtt                                                                                                                              >  2:214484/1‑138 (MQ=255)
            cAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGaa                                                                                                                         >  2:4384/1‑139 (MQ=255)
              gagaAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAacg                                                                                                                       <  4:360192/139‑1 (MQ=255)
                    aCGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGAtt                                                                                                                 >  6:41699/1‑139 (MQ=255)
                    aCGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGAtt                                                                                                                 >  6:36232/1‑139 (MQ=255)
                           tGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCt                                                                                                          >  6:56268/1‑139 (MQ=255)
                           tGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGACACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCt                                                                                                          >  5:235181/1‑139 (MQ=255)
                            ggATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCATAGAGTAATTCGCGATGTTGAGAACGACGATTTGCTCCt                                                                                                          <  8:245210/138‑1 (MQ=255)
                                tCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCAt                                                                                                     >  4:299463/1‑139 (MQ=255)
                                  aTTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTTATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTa                                                                                                   <  2:395299/139‑1 (MQ=255)
                                  aTTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCAtt                                                                                                    >  6:279564/1‑138 (MQ=255)
                                     ggAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTaaa                                                                                                 <  4:323960/138‑1 (MQ=255)
                                       aaaTTCAGGGTCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGt                                                                                              >  6:217194/1‑139 (MQ=255)
                                            cAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGc                                                                                           >  3:182864/1‑137 (MQ=255)
                                                  cccGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAg                                                                                    >  5:30452/1‑138 (MQ=255)
                                                            cTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGACCGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAg                                                                          >  6:81563/1‑138 (MQ=255)
                                                            cTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAg                                                                          >  6:81560/1‑138 (MQ=255)
                                                               aaGAGATTAAAAAGCGGATGTGTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGaaa                                                                       <  5:282517/138‑1 (MQ=255)
                                                                agagATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAAtt                                                                     <  6:169184/139‑1 (MQ=255)
                                                                agagATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAAtt                                                                     <  1:4384/139‑1 (MQ=255)
                                                                agagATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAAtt                                                                     <  5:217194/139‑1 (MQ=255)
                                                                 gagaTTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATtg                                                                    >  5:50318/1‑139 (MQ=255)
                                                                   gaTTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATtgtg                                                                  <  3:300888/139‑1 (MQ=255)
                                                                    aTTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATACCCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTgtgt                                                                 >  4:76158/1‑139 (MQ=255)
                                                                          aaGCGGATGTTTGTCTTCTAAGATCTTGGCCAAATTGATCAGCGTGCAATTAAGCGAGTCATTCGGGATGTTGAGAAAGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGATcaaca                                                           <  3:42076/139‑1 (MQ=255)
                                                                          aaGCGGATGTTAGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTcaaca                                                           <  4:57899/139‑1 (MQ=255)
                                                                             cGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAata                                                        <  6:30452/139‑1 (MQ=255)
                                                                              ggATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAatat                                                       <  8:226505/139‑1 (MQ=255)
                                                                              ggATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAatat                                                       >  7:17186/1‑139 (MQ=255)
                                                                                   tttGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTca                                                   <  3:174762/138‑1 (MQ=255)
                                                                                   tttGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTca                                                   <  1:31289/138‑1 (MQ=255)
                                                                                     tGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTcaca                                                 <  5:56268/138‑1 (MQ=255)
                                                                                     tGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTcaca                                                 <  5:39551/138‑1 (MQ=255)
                                                                                       tCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCg                                              >  1:80862/1‑139 (MQ=255)
                                                                                         ttCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATT‑AAGGTTGAAAGCGAGGAAGTCAAAGAAATTGTGTTAAACAATATGTCACAGCGTAt                                           >  8:62939/1‑139 (MQ=255)
                                                                                           cGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGt                                             <  1:381237/136‑1 (MQ=255)
                                                                                           cGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTa                                            <  5:75778/137‑1 (MQ=255)
                                                                                                   ttGTCACACTTGATAACCCTGCAATTCAGCGAGTCAGTCGCGATGTTGAGAACGACGATTTGCTCCTTTCAGTAAAGGATGCAAGCGAGGAAGTCAAAGAAATTGTGTTCCACAATATGTCACAGCGTATGGTTGAAAc                                  >  8:256999/1‑139 (MQ=255)
                                                                                                        acacTTGATAACCGTGCAATTCAGAGAGTCATTCGCGAAGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAACAAATTGTGATCAACAATATGTCACAGCGTATGGTTGAAACCTTc                              >  5:20979/1‑138 (MQ=255)
                                                                                                         cacTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCaa                            <  1:214484/139‑1 (MQ=255)
                                                                                                          acTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTcaaca                                                           >  5:337838/1‑107 (MQ=255)
                                                                                                          acTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTcaaca                                                           <  6:337838/107‑1 (MQ=255)
                                                                                                          acTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCa                             >  5:44243/1‑137 (MQ=255)
                                                                                                                  aCCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGaaa                    <  7:104160/138‑1 (MQ=255)
                                                                                                                   ccGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATg                  <  5:279564/139‑1 (MQ=255)
                                                                                                                    cGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTcaa                                                             <  5:321665/95‑1 (MQ=255)
                                                                                                                    cGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTcaa                                                             >  6:321665/1‑95 (MQ=255)
                                                                                                                    cGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTca                                                   <  6:277283/105‑1 (MQ=255)
                                                                                                                    cGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTca                                                   >  5:277283/1‑105 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGaa                                    <  3:142966/118‑1 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGaa                                    >  4:142966/1‑118 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCaa                            <  4:130743/126‑1 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCaa                            >  3:130743/1‑126 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  5:36113/1‑139 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  6:318588/1‑139 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  6:169051/1‑139 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  5:406347/1‑139 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  2:394431/1‑139 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  2:300735/1‑139 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  8:175435/1‑139 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCt                                                                                                          >  1:288125/1‑48 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCt                                                                                                          <  2:288125/48‑1 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAaaga                         <  7:254726/129‑1 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAaaga                         >  8:254726/1‑129 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  8:238040/1‑139 (MQ=255)
                                                                                                                      tGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTATCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg               >  2:346683/1‑139 (MQ=255)
                                                                                                                          aTTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTa           <  6:50318/139‑1 (MQ=255)
                                                                                                                           ttCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTAt          <  6:244650/139‑1 (MQ=255)
                                                                                                                                agagTCATTAGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGcc     <  2:315201/139‑1 (MQ=255)
                                                                                                                                   gTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTg  <  1:394431/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                         
TAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTG  >  CP006881/1657788‑1658057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A