Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9101112 F55 I0 R1 156 414.6 11700970 99.6% 11654166 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,657,920 T→A 5.1% V255D (GTC→GAC)  U712_08520 → Flagellar motor switch protein FliG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,657,9200TA 5.1% 1055.6 / 35.6 311V255D (GTC→GAC) U712_08520Flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base T (181/114);  new base A (11/5);  total (192/119)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-01

Only 100 of 314 total aligned reads displayed.

GAAGTGTTAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACGTTTGAAAG  >  CP006881/1657772‑1658069
                                                                                                                                                    |                                                                                                                                                     
gAAGTGTTAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTc                                                                                                                                                      <  2:1271115/150‑1 (MQ=255)
  aGTGTTAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCAt                                                                                                                                                    <  2:3116167/150‑1 (MQ=255)
   gtgtTAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCAtt                                                                                                                                                   >  1:1092688/1‑150 (MQ=255)
   gtgtTAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCAtt                                                                                                                                                   >  1:638161/1‑150 (MQ=255)
    tgtTAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTc                                                                                                                                                  >  2:5202028/1‑150 (MQ=255)
     gtTAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTcg                                                                                                                                                 >  2:2489881/1‑150 (MQ=255)
      ttAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTcgc                                                                                                                                                <  2:1735144/150‑1 (MQ=255)
          aCGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATg                                                                                                                                            <  2:4930351/150‑1 (MQ=255)
          aCGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATg                                                                                                                                            <  2:4065114/150‑1 (MQ=255)
          aCGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATg                                                                                                                                            <  2:2067874/150‑1 (MQ=255)
               gTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGTTAACCGTGCAATTCAGAGAGTCATTCGCGATGTTgag                                                                                                                                       >  1:1626305/1‑150 (MQ=255)
                tAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTgaga                                                                                                                                      <  2:1666473/150‑1 (MQ=255)
                 aGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGaa                                                                                                                                     >  1:1400209/1‑150 (MQ=255)
                  gACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAac                                                                                                                                    <  2:3995846/150‑1 (MQ=255)
                   aCAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAacg                                                                                                                                   >  1:3616552/1‑150 (MQ=255)
                         ggACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGAtt                                                                                                                             <  2:1963796/150‑1 (MQ=255)
                           aCAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTg                                                                                                                           >  2:3011906/1‑150 (MQ=255)
                                gaagaCGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCt                                                                                                                      >  2:689549/1‑150 (MQ=255)
                                gaagaCGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCt                                                                                                                      <  2:1458539/150‑1 (MQ=255)
                                    aCGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCa                                                                                                                  <  2:5109580/150‑1 (MQ=255)
                                        tCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTaa                                                                                                              >  2:4418857/1‑150 (MQ=255)
                                             gATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGtt                                                                                                         >  1:4587867/1‑150 (MQ=255)
                                              aTTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTg                                                                                                        <  2:5264819/150‑1 (MQ=255)
                                                tCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCa                                                                                                      >  1:331903/1‑150 (MQ=255)
                                                  aTTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAg                                                                                                    >  1:5113166/1‑150 (MQ=255)
                                                    tGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGGCGATTTGCTCCTTTCATTAAAGGTTGCAAGct                                                                                                  >  2:2173204/1‑149 (MQ=255)
                                                      gAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCAAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTACAGGTTGCAAGCGAg                                                                                                >  2:122916/1‑150 (MQ=255)
                                                       aaaTTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAgg                                                                                               <  2:4154745/150‑1 (MQ=255)
                                                       aaaTTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGCCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAgg                                                                                               <  1:3037093/150‑1 (MQ=255)
                                                         aTTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGaa                                                                                             >  2:2898274/1‑150 (MQ=255)
                                                         aTTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGaa                                                                                             >  1:883027/1‑150 (MQ=255)
                                                         aTTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGaa                                                                                             >  1:5538227/1‑150 (MQ=255)
                                                          ttCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAg                                                                                            >  2:3562516/1‑150 (MQ=255)
                                                           tCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGt                                                                                           >  2:913579/1‑150 (MQ=255)
                                                             aGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCa                                                                                         >  2:2316605/1‑150 (MQ=255)
                                                                aTCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAg                                                                                      >  2:1541379/1‑150 (MQ=255)
                                                                     gATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAAtt                                                                                 >  2:1135217/1‑150 (MQ=255)
                                                                         ttGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTgtgt                                                                             >  2:725632/1‑150 (MQ=255)
                                                                         ttGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGCTTGTAAGAGAGGAAGTCAAAGAAATTgtgt                                                                             >  1:3813408/1‑150 (MQ=255)
                                                                          tGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATCTGCTCCTTTCATTAAAGGTTGGAAGCGAGGAAGTCAAAGAAATTGGGtt                                                                            >  1:2837433/1‑150 (MQ=255)
                                                                          tGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGtt                                                                            >  2:3418530/1‑150 (MQ=255)
                                                                                agagATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTcaacaa                                                                      <  2:3850914/150‑1 (MQ=255)
                                                                                 gagaTTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAat                                                                     >  2:2184696/1‑150 (MQ=255)
                                                                                  agaTTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAata                                                                    >  2:827614/1‑150 (MQ=255)
                                                                                   gaTTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAatat                                                                   <  2:3462448/150‑1 (MQ=255)
                                                                                    aTTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATg                                                                  >  2:3979259/1‑150 (MQ=255)
                                                                                      tAAAAAGCGGATGTTTGTTTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCCCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTc                                                                <  1:5427098/150‑1 (MQ=255)
                                                                                      tAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTc                                                                >  1:5511080/1‑150 (MQ=255)
                                                                                      tAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTc                                                                <  2:638161/150‑1 (MQ=255)
                                                                                        aaaaGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTcac                                                              >  1:986861/1‑150 (MQ=255)
                                                                                        aaaaGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTcac                                                              >  1:4069101/1‑150 (MQ=255)
                                                                                         aaaGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTcaca                                                             >  2:3522991/1‑150 (MQ=255)
                                                                                          aaGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAg                                                            >  2:1365908/1‑150 (MQ=255)
                                                                                             cGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGt                                                         >  2:3652134/1‑150 (MQ=255)
                                                                                             cGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGt                                                         >  1:4681263/1‑150 (MQ=255)
                                                                                              ggATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGGGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTa                                                        <  2:3189240/150‑1 (MQ=255)
                                                                                               gATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTAt                                                       >  1:3986722/1‑150 (MQ=255)
                                                                                                   tttGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGtt                                                   >  2:2083140/1‑150 (MQ=255)
                                                                                                     tGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGa                                                 >  1:1246731/1‑150 (MQ=255)
                                                                                                        cTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAAc                                              >  2:2117747/1‑150 (MQ=255)
                                                                                                          tCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCt                                            >  1:4475302/1‑150 (MQ=255)
                                                                                                           cGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCtt                                           <  2:5505826/150‑1 (MQ=255)
                                                                                                           cGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCtt                                           <  2:1987231/150‑1 (MQ=255)
                                                                                                           cGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCtt                                           <  2:331903/150‑1 (MQ=255)
                                                                                                           cGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCtt                                           <  1:2059557/150‑1 (MQ=255)
                                                                                                            gAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTc                                          <  2:429608/150‑1 (MQ=255)
                                                                                                             aaGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCa                                         <  1:5683867/150‑1 (MQ=255)
                                                                                                              aGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCaa                                        <  1:1778785/150‑1 (MQ=255)
                                                                                                                atatTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAaag                                      >  1:4914530/1‑150 (MQ=255)
                                                                                                                 tatTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAaaga                                     <  1:2538095/150‑1 (MQ=255)
                                                                                                                  atTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAaagaa                                    >  2:4905178/1‑150 (MQ=255)
                                                                                                                   ttGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAaagaag                                   <  1:2608780/150‑1 (MQ=255)
                                                                                                                     gTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAgaagaa                                 <  2:4490507/150‑1 (MQ=255)
                                                                                                                      tCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGTGAACTACGATTTGCTCCTTTCATTAAAGGATGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGaaa                                >  1:4801731/1‑150 (MQ=255)
                                                                                                                       cacaCTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAAt                               <  2:4221407/150‑1 (MQ=255)
                                                                                                                       cacaCTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAAt                               >  1:4410741/1‑150 (MQ=255)
                                                                                                                       cacaCTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAAt                               >  1:3513635/1‑150 (MQ=255)
                                                                                                                       cacaCTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAAt                               >  2:2409512/1‑150 (MQ=255)
                                                                                                                         cacTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATgg                             <  1:2465475/150‑1 (MQ=255)
                                                                                                                          acTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGa                            >  1:1575421/1‑150 (MQ=255)
                                                                                                                           cTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAg                           <  2:3692167/150‑1 (MQ=255)
                                                                                                                            ttGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGGACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCCAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGt                          >  2:5810489/1‑150 (MQ=255)
                                                                                                                            tcaaTAACCCTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGATTGCAAGCGAGGAAGACAAAGAAATTCTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGt                          <  2:4801731/147‑1 (MQ=255)
                                                                                                                             tGATAACCGTGCAATTCAGAGAGACATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGtt                         <  2:5554246/150‑1 (MQ=255)
                                                                                                                              gATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGttt                        <  1:3559928/150‑1 (MQ=255)
                                                                                                                                    cGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGc                  >  2:3911468/1‑150 (MQ=255)
                                                                                                                                    cGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGc                  >  2:1777023/1‑150 (MQ=255)
                                                                                                                                        cAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTg              <  1:2263351/150‑1 (MQ=255)
                                                                                                                                         aaTTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGt             <  1:4929897/150‑1 (MQ=255)
                                                                                                                                          aTTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTa            <  2:3767778/150‑1 (MQ=255)
                                                                                                                                          aTTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTa            >  1:1297993/1‑150 (MQ=255)
                                                                                                                                           ttCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTTCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGATATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTAc           <  1:5279308/150‑1 (MQ=255)
                                                                                                                                           ttCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTAc           <  1:1170349/150‑1 (MQ=255)
                                                                                                                                            tCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACg          <  2:5690588/150‑1 (MQ=255)
                                                                                                                                              agagagTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACGtt        >  2:460841/1‑150 (MQ=255)
                                                                                                                                               gagagTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACGttt       >  2:2092186/1‑150 (MQ=255)
                                                                                                                                                agagTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTACAGGTTGCAGGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACGTTTg      >  2:3943425/1‑150 (MQ=255)
                                                                                                                                                agagTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACGTTTg      >  2:430076/1‑150 (MQ=255)
                                                                                                                                                 gagTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACGTTTGa     >  2:4345384/1‑150 (MQ=255)
                                                                                                                                                    tCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACGTTTGAAAg  <  2:1092688/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAAGTGTTAAACGGGGTAGACAGAGGGACAGAGAAGACGATCTTGGATTCATTGGAAATTCAGGATCCCGATCTTGCTGAAGAGATTAAAAAGCGGATGTTTGTCTTCGAAGATATTGTCACACTTGATAACCGTGCAATTCAGAGAGTCATTCGCGATGTTGAGAACGACGATTTGCTCCTTTCATTAAAGGTTGCAAGCGAGGAAGTCAAAGAAATTGTGTTCAACAATATGTCACAGCGTATGGTTGAAACCTTCAAAGAAGAAATGGAGTTTATGGGCCCTGTACGTTTGAAAG  >  CP006881/1657772‑1658069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A