Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9101112 F55 I0 R1 156 414.6 11700970 99.6% 11654166 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,862,707 T→C 34.8% A27A (GCT→GCC)  U712_09270 → Uncharacterized protein yncE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,862,7070TC34.8% 318.8 / inf 364A27A (GCT→GCC) U712_09270Uncharacterized protein yncE
Reads supporting (aligned to +/- strand):  ref base T (132/105);  new base C (85/41);  total (218/146)
Fisher's exact test for biased strand distribution p-value = 3.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 367 total aligned reads displayed.

CCATAAACCTAACATCAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCATTTTCGTTGA  >  CP006881/1862558‑1862856
                                                                                                                                                     |                                                                                                                                                     
ccATAAACCTAACATCAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCt                                                                                                                                                       <  2:5716345/150‑1 (MQ=255)
  aTAAACCTAACATCAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTaa                                                                                                                                                     <  2:854156/150‑1 (MQ=255)
   tAAACCTAACATCAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAAt                                                                                                                                                    >  2:704085/1‑150 (MQ=255)
      aCCTAACATCAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATtat                                                                                                                                                 <  2:3038854/150‑1 (MQ=255)
            cATCAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTgg                                                                                                                                           <  2:1924524/150‑1 (MQ=255)
              tCAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCa                                                                                                                                         <  1:3670061/150‑1 (MQ=255)
               cAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCaa                                                                                                                                        >  2:5387916/1‑150 (MQ=255)
                agagTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCaaa                                                                                                                                       <  2:4627602/150‑1 (MQ=255)
                agagTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCaaa                                                                                                                                       >  2:1173296/1‑150 (MQ=255)
                     atataATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGt                                                                                                                                  <  2:1638900/150‑1 (MQ=255)
                     atataATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGt                                                                                                                                  >  1:1287683/1‑150 (MQ=255)
                      tataATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGACTTAGTTTTCAAATACCGAATGAATATGGAAATTTTGTATGCAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTg                                                                                                                                 <  2:3218917/150‑1 (MQ=255)
                       ataATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTgg                                                                                                                                <  1:5504327/150‑1 (MQ=255)
                       ataATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTgg                                                                                                                                <  1:3250307/150‑1 (MQ=255)
                       ataATNATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTgg                                                                                                                                >  1:5829760/1‑150 (MQ=255)
                        taATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTggg                                                                                                                               <  2:537156/150‑1 (MQ=255)
                         aaTTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTgggg                                                                                                                              <  2:4459431/150‑1 (MQ=255)
                           ttATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGaa                                                                                                                            <  2:5760411/150‑1 (MQ=255)
                            tataCTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAAt                                                                                                                           <  2:4529611/150‑1 (MQ=255)
                              taCTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCt                                                                                                                         <  1:96120/150‑1 (MQ=255)
                               aCTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCtt                                                                                                                        <  1:1573330/150‑1 (MQ=255)
                               aCTCGGATTCACAAAAATCGTTTAAGTAAGGCGTAGACATGAATAGCGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGCAGAATACTGTAGCCGGTAGAAATAGCTAATTATAGGTGGCAAGCGAGTGGTGAATCtt                                                                                                                        >  2:5156686/1‑150 (MQ=255)
                                 tCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTAt                                                                                                                      >  1:2542197/1‑150 (MQ=255)
                                   gTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTAttt                                                                                                                    >  1:2965638/1‑150 (MQ=255)
                                       tCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTttgtt                                                                                                                <  2:3374673/150‑1 (MQ=255)
                                        cacaAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTttgttg                                                                                                               >  1:2751952/1‑150 (MQ=255)
                                        cacaAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTttgttg                                                                                                               >  2:4390892/1‑150 (MQ=255)
                                           aaaaaTCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAg                                                                                                            >  2:5436688/1‑150 (MQ=255)
                                           aaaaaTCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCATATTTTGTTGTAg                                                                                                            >  2:2817541/1‑150 (MQ=255)
                                           aaaaaTCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAg                                                                                                            >  1:3684815/1‑150 (MQ=255)
                                             aaaTCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGaa                                                                                                          >  1:1629565/1‑150 (MQ=255)
                                              aaTCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAg                                                                                                         <  2:1077320/150‑1 (MQ=255)
                                              aaTCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAg                                                                                                         >  1:3110606/1‑150 (MQ=255)
                                                  gTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGgaga                                                                                                     >  2:2070180/1‑150 (MQ=255)
                                                      aagtaagGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTc                                                                                                 <  1:2040063/150‑1 (MQ=255)
                                                         taagGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCgat                                                                                              <  2:3873940/150‑1 (MQ=255)
                                                          aagGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCgatg                                                                                             >  2:1248894/1‑150 (MQ=255)
                                                          aagGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCgatg                                                                                             >  2:3787581/1‑150 (MQ=255)
                                                                     aTGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCtt                                                                                  <  1:4560975/150‑1 (MQ=255)
                                                                      tGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCttt                                                                                 <  1:469208/150‑1 (MQ=255)
                                                                       gAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCtttt                                                                                >  1:809767/1‑150 (MQ=255)
                                                                         aTAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTc                                                                              >  2:518587/1‑150 (MQ=255)
                                                                          tAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCa                                                                             <  2:1286881/150‑1 (MQ=255)
                                                                            ggggAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATg                                                                           <  1:3189513/150‑1 (MQ=255)
                                                                                agttagttTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTa                                                                       >  1:4003947/1‑150 (MQ=255)
                                                                                 gttagttTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTAc                                                                      <  2:3451319/150‑1 (MQ=255)
                                                                                      ttttCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGGCTACGAAAt                                                                 >  2:2795732/1‑150 (MQ=255)
                                                                                       tttCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCATATTTTGTTGTAGAAGAAAAGCTCGATGATGAGGGGCTTTTTCATGACTACGAAAtt                                                                >  1:2905477/1‑150 (MQ=255)
                                                                                        ttCAAATACCGAATGAATATGGAAATTTTCTATGTAGAATACTGCAGCCGCTAGAAATAGCTAATTAAAGGTGGCAAACGTGTGGGGAATCTTATTTTGTTGTAGTAGGAGAGCTCGATGATGAGGAGCTTTTTCATGTCTACGAAAttg                                                               >  1:5288045/1‑150 (MQ=255)
                                                                                          cAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAAttgtt                                                             >  2:2931527/1‑150 (MQ=255)
                                                                                          cAAATACCGAATGAATATGGAAATTTCCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAAttgtt                                                             <  1:4889126/150‑1 (MQ=255)
                                                                                             aTACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAg                                                          <  1:5130657/150‑1 (MQ=255)
                                                                                             aTACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAg                                                          >  1:421649/1‑150 (MQ=255)
                                                                                               aCCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAggg                                                        >  2:5843538/1‑150 (MQ=255)
                                                                                                  gaatgaatATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACCAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCCATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGc                                                     >  1:1839529/1‑150 (MQ=255)
                                                                                                   aatgaatATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCa                                                    >  2:1760356/1‑150 (MQ=255)
                                                                                                   aatgaatATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCa                                                    <  1:2943501/150‑1 (MQ=255)
                                                                                                      gaatATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGtt                                                 <  1:2629304/150‑1 (MQ=255)
                                                                                                       aatATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGttt                                                <  2:4080697/150‑1 (MQ=255)
                                                                                                       aatATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGttt                                                >  2:4993738/1‑150 (MQ=255)
                                                                                                       aatATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGttt                                                >  2:4236643/1‑150 (MQ=255)
                                                                                                        atatGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGtttt                                               >  1:4172646/1‑150 (MQ=255)
                                                                                                        atatGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAACTGTTGAGGGGGCAGtttt                                               >  1:1856027/1‑150 (MQ=255)
                                                                                                         tatGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGttttt                                              <  2:5549821/150‑1 (MQ=255)
                                                                                                         tatGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAAGTGTTGAGGGGGCAGttttt                                              >  1:4976573/1‑150 (MQ=255)
                                                                                                            ggAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAg                                           <  1:228687/150‑1 (MQ=255)
                                                                                                               aaTTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGcaa                                        >  2:5656748/1‑150 (MQ=255)
                                                                                                                    tCTATGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAAtt                                   <  1:704085/150‑1 (MQ=255)
                                                                                                                     cTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTa                                  >  2:949804/1‑150 (MQ=255)
                                                                                                                      tATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTaa                                 >  2:5032200/1‑150 (MQ=255)
                                                                                                                      tATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTaa                                 >  2:2148856/1‑150 (MQ=255)
                                                                                                                       aTGGAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGTGTGGGGAGTTTTTGTGCAACAATTaaa                                >  2:161944/1‑150 (MQ=255)
                                                                                                                         ggAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTaaaaa                              >  2:2492985/1‑150 (MQ=255)
                                                                                                                         ggAGAATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTaaaaa                              >  2:2273128/1‑150 (MQ=255)
                                                                                                                          gagaATACTGCAGCCGGAAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAAc                             <  2:2012021/150‑1 (MQ=255)
                                                                                                                           agaATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACg                            >  1:650698/1‑150 (MQ=255)
                                                                                                                            gaATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGGGGGGGCAGTTTTTGAGCAACAATTAAAAACGa                           >  2:3617637/1‑150 (MQ=255)
                                                                                                                            gaATACTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGa                           >  1:4271489/1‑150 (MQ=255)
                                                                                                                             aaTACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGaa                          >  2:113156/1‑150 (MQ=255)
                                                                                                                                acagCAGCGGGTAGAAATAGCTAATTATAGGTGGTAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAAtga                       <  1:5156686/147‑3 (MQ=255)
                                                                                                                                aCTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCa                       <  2:4679667/150‑1 (MQ=255)
                                                                                                                                aCTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCa                       >  2:5245568/1‑150 (MQ=255)
                                                                                                                                 cTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAg                      <  1:5436688/150‑1 (MQ=255)
                                                                                                                                 cTGCAGCCGGTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAg                      >  1:1484166/1‑150 (MQ=255)
                                                                                                                                  tGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGt                     <  1:3386492/150‑1 (MQ=255)
                                                                                                                                     aGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGTGCAACAATTAAAAACGAATCAGTACt                  >  2:3000426/1‑150 (MQ=255)
                                                                                                                                       ccGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACtat                >  1:3912760/1‑150 (MQ=255)
                                                                                                                                       ccGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACtat                >  2:2700795/1‑150 (MQ=255)
                                                                                                                                         ggTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATAc              >  1:1022590/1‑150 (MQ=255)
                                                                                                                                         ggTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATAc              <  1:105662/150‑1 (MQ=255)
                                                                                                                                         ggTAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATAc              >  1:3733527/1‑150 (MQ=255)
                                                                                                                                           tAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCa            <  1:2155434/150‑1 (MQ=255)
                                                                                                                                           tAGAAATAGCCAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCa            >  2:4795065/1‑150 (MQ=255)
                                                                                                                                            aGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCAt           <  2:1508518/150‑1 (MQ=255)
                                                                                                                                              aaaTAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCAttt         <  2:4904626/150‑1 (MQ=255)
                                                                                                                                                 tAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCATTTTCg      >  2:3676102/1‑150 (MQ=255)
                                                                                                                                                  aGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCATTTTCGt     >  1:4640172/1‑150 (MQ=255)
                                                                                                                                                   gCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCATTTTCGtt    >  2:2440507/1‑150 (MQ=255)
                                                                                                                                                   gCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCATTTTCGtt    <  1:5648596/150‑1 (MQ=255)
                                                                                                                                                    cTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCATTTTCGTTg   >  2:257081/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATAAACCTAACATCAGAGTATATAATTATACTCGTATTCACAAAAATCGTTTAAGTAAGGGGTATACATGAATAGGGGAGTTAGTTTTCAAATACCGAATGAATATGGAAATTTTCTATGGAGAATACTGCAGCCGGTAGAAATAGCTAATTATAGGTGGCAAACGAGTGGGGAATCTTATTTTGTTGTAGAAGGAGAGCTCGATGATGAGGAGCTTTTTCATGACTACGAAATTGTTGAGGGGGCAGTTTTTGAGCAACAATTAAAAACGAATCAGTACTATACCATTTTCGTTGA  >  CP006881/1862558‑1862856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A