Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F56 I0 R1
|
218 |
83.1 |
3292392 |
95.3% |
3137649 |
126.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
1,103,575 |
A→C |
26.2% |
K245N (AAA→AAC) |
U712_05435 → |
ATP‑dependent helicase/nuclease subunit A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 1,103,575 | 0 | A | C | 26.2%
| 80.1
/ 9.1
| 62 | K245N (AAA→AAC) | U712_05435 | ATP‑dependent helicase/nuclease subunit A |
| Reads supporting (aligned to +/- strand): ref base A (30/14); new base C (2/14); total (32/30) |
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGTATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCAA > CP006881/1103450‑1103709
|
ttGTATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGcc > 5:345116/1‑139 (MQ=255)
ttGTATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTATTGCGCGCGCTTGAGCTGACGAACGCGCCGGGCGGcc > 1:171656/1‑139 (MQ=255)
tgTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCCGcgccg > 4:34813/1‑139 (MQ=255)
tATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCcgcg > 7:329021/1‑139 (MQ=255)
tCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTg > 5:152136/1‑139 (MQ=255)
cAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCcgcgggg > 1:269395/1‑136 (MQ=255)
aaGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGAc > 1:19588/1‑138 (MQ=255)
agCGCCATCGAGGAGCTGCCTTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCCGGCGGCCCGGCGCCCCGTGCGTACAAtt > 1:36118/1‑139 (MQ=255)
gcgcCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTTCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGCGCTGACGACAGCGCCGGGCGGCCCGGCCCCGCGGGGGGGCAAttt > 7:156989/1‑139 (MQ=255)
gcgcCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCGGACAAttt > 1:220890/1‑139 (MQ=255)
cgcCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCGGCCCAtttt > 3:327960/1‑139 (MQ=255)
cATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCtt > 8:338416/1‑139 (MQ=255)
cATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCGGACAATTTTCtt > 4:237404/1‑139 (MQ=255)
cATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGGGGCCCATTTTCtg > 1:349798/1‑138 (MQ=255)
ggatCTTCCGTTTTATCATTTTGTTTATCAATATATTGATTTGGTGCTAAACGGGGCGATGGAACAGAGATTGGGCGGGGTTGAGCTGACGACCGGGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTgatgat < 2:234316/139‑1 (MQ=255)
tagCTGGGGTTTTATTAATTTGTCAATTAAGTTTTTGAATTGGTGATTTTGGGGTCTATGTAAAAGCTCTTGGGCTTGCTTGTGGTTAGGAACGCGCGGGGGGGCCCGGCGCCGCGTGCTGACAATTTTCTTgatgat < 6:345116/137‑1 (MQ=255)
gcgCTGCCGTTTTATCAATATGTAAAATATGTTATTGCAAGGGTGCTTACGGGGGCGAAGGAAATGCTCTGGCGCGCGCTTGCGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATc < 4:274970/137‑1 (MQ=255)
gCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTAATTATGATCTTgga > 2:85666/1‑137 (MQ=255)
cGTTTTATCAATATGACAAATAAGATATTGCAATGGGGCTTCACGGGCCGACGTAAACGCTCTGGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCa < 5:128194/139‑1 (MQ=255)
tttATAAATATGACAACGAATTTATTGCATTGGTGCTTACCGGGGCTAAGCAAAAGCTCTGGCGCGCGCTTGAGCTGACGAACGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGa < 6:141115/138‑1 (MQ=255)
atatTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGGGCGGACAATTTTCTTGATGATCTTGCTCAGATGGATGAACTGATTCAGCAt > 5:233748/1‑137 (MQ=255)
gTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGAttt > 5:93934/1‑137 (MQ=255)
gTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGAttt > 7:190301/1‑137 (MQ=255)
gTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGAttt > 3:76968/1‑137 (MQ=255)
gtataCGGGCCTAAGGAAAGTCTGCTGCGCGCGCTTGAGCGTCCGAATGCGCCGGGCGGCCCGGCGCCGCGTGCTGTCAATTTTCTTGATGATCTTGCTCAGATTGATGAACTTATTCAGCATCAGGACGATTTCAGTg < 5:213197/135‑1 (MQ=255)
cgtaccGGGGCGATGGAAACGCTCTTGCGCGCGCTTGCGCGCACTAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg < 7:375214/134‑1 (MQ=255)
cTTACCGGGGCGACGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg < 6:128221/139‑1 (MQ=255)
cTTAACGGGGCGAAGTAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg < 5:151010/139‑1 (MQ=255)
cTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGTCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGt > 4:309527/1‑138 (MQ=255)
cTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGGTGACTATTTTCTTGATGATCTTGGTCAGATTGATTAACTGATTCAGCATCAGGACGATTTAAGt > 8:73067/1‑138 (MQ=255)
cTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGCCAATTTTATTGATGATCTTTGTCAGATTGAAGAACTGATTAAGCATCAGGACGATTTCAGt > 5:129379/1‑138 (MQ=255)
cTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTATTGATGATCTTGCTCAGATTGATGAACTGATTCAGCCTCACGAAGATTTAAGt > 5:376537/1‑138 (MQ=255)
cTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCCCGTGCTGCCAAATTTATTGTTTTTCATGGTCACAAGGATGAACGGATTCAGCAGAAGGACCATTAAAGTg > 5:252824/1‑139 (MQ=255)
attACCGGGCCGAAGCAAATCCTCTTGCGCGCGCTTTAGCCGACGAAAGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg < 6:411589/138‑1 (MQ=255)
ccgggggCCACGCAAATGGTCGTGCGGGCGCTGGTGCGGACTACCGGGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 1:141790/135‑1 (MQ=255)
caCGGGGCTACGGAAACGCTCTTGCGCGCGCGTGAGCGGACGAAAGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 2:19588/137‑1 (MQ=255)
acgggggCTAAGGAAACGCTCTTGCGCGGGCTTGAGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 4:327960/135‑1 (MQ=255)
acgggggCGATCGAAACGCTCGTGGGCGCGCGTGAGCTGACGACCGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAGTTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 3:330218/135‑1 (MQ=255)
accGGGCCCAAGCAAACGCTCTGCCGCGCGCTGGAGCTCACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 3:27056/136‑1 (MQ=255)
aaCGGGGCGATGGAAACGGGGTGGGGCGGGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 8:190301/138‑1 (MQ=255)
aaCGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGTCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 3:309527/138‑1 (MQ=255)
aaCGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 8:329021/138‑1 (MQ=255)
aaCGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 7:338416/138‑1 (MQ=255)
aaCGGGGCCAGGCAAAAGCGCTTGCGGGCGCTTGAGCGGACGAACGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa < 6:233748/138‑1 (MQ=255)
gggCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTTCTGACAATTTTATTTAAGATATTTCTCACATTGTAGAAATTATTAAGCATAAGGACCATTTGAGTGAAcaaaa > 4:398664/1‑135 (MQ=255)
gggCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACtata > 7:48742/1‑139 (MQ=255)
ggCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGGTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACAGATTCAGCATCAGGACGATTTAAGTGAACtata > 5:340929/1‑138 (MQ=255)
ggCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCCTGCTGACAAATTTCTTGATTATCTTGCTCAGATTGATGAACTGAATCAGCATCAGGACGATTTCAGTGAACtata > 7:388767/1‑138 (MQ=255)
cGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGCGGACAATTTTCTTGATGATCTTGCTCAGTTTGATGTACGTATTCAGCATCAGGACGATTTCAGTGAAAgatat > 2:193805/1‑137 (MQ=255)
aaGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGAGGATTTCAGTGAACTATATAAGCGGGTGc > 2:111184/1‑139 (MQ=255)
aaGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg > 1:234462/1‑121 (MQ=255)
aaGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg < 2:234462/121‑1 (MQ=255)
tGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGt < 6:152136/139‑1 (MQ=255)
tGCGCGCGCTGGCGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGt < 4:76968/139‑1 (MQ=255)
ggggcgcggGTGCGCGGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGt < 8:156989/136‑1 (MQ=255)
ggcgcgcgcTTGAGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGt < 2:220890/138‑1 (MQ=255)
cgcccccgcTTGAGCTGACTAACGCGCGGGGGGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGt < 6:188042/133‑1 (MQ=255)
ccgcgcgcTCGCGCGGACGACCGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGtc < 1:148030/138‑1 (MQ=255)
cgcgcgtTTGCCCTGCCGACGGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGtc < 3:146226/138‑1 (MQ=255)
gcTTGCTCTGACGAACGCTCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCtttt < 6:402627/139‑1 (MQ=255)
aCGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGcc > 7:45820/1‑138 (MQ=255)
cGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCaa < 8:84776/139‑1 (MQ=255)
|
TTGTATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCAA > CP006881/1103450‑1103709
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
