Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F54 I0 R2 227 88.8 3424874 95.5% 3270754 127.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,103,575 A→C 33.1% K245N (AAA→AAC)  U712_05435 → ATP‑dependent helicase/nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,103,5750AC33.1% 44.0 / 8.3 47K245N (AAA→AAC) U712_05435ATP‑dependent helicase/nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (27/4);  new base C (2/12);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 4.53e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCA  >  CP006881/1103443‑1103708
                                                                                                                                    |                                                                                                                                     
tGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAAAGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCg                                                                                                                                 >  1:295310/1‑139 (MQ=255)
       ttGTATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCGTGAGCTGACGAAAGCGCCGGGCGGcc                                                                                                                          >  2:113676/1‑139 (MQ=255)
          tATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGcc                                                                                                                          >  3:383676/1‑136 (MQ=255)
               tgTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGcgccg                                                                                                                  >  7:388872/1‑139 (MQ=255)
                      gAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGcg                                                                                                            >  1:373867/1‑137 (MQ=255)
                              cgcCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGGGGACGAtttt                                                                                                   >  5:357562/1‑139 (MQ=255)
                                 cATCGAGGAGCTGCCGTTTTATAAATAATTCAAATAAAATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGCCGAAAGCGCCGGGCCGCCCCGCGCCCCCGGGTGGCAAtttt                                                                                                   >  4:32740/1‑136 (MQ=255)
                                    cGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTAtt                                                                                                >  4:376002/1‑136 (MQ=255)
                                       ggagGTGCGTTTTTATAAATATGTCAAATAAGATATTGCATTGGTGCTTACCGGGGCGAAGTAAAAGCTCTTGCGCGCGGTTGTGCTGACTAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTgatgat                                                                                          <  2:258831/139‑1 (MQ=255)
                                           cTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGCTGGCAAATTTGTTGATGGTCttt                                                                                      >  1:381522/1‑138 (MQ=255)
                                           cTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGCTGACAATTTTATTGATGATCTTg                                                                                      >  5:200882/1‑139 (MQ=255)
                                             gCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCt                                                                                    >  5:429419/1‑139 (MQ=255)
                                             gCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGCTGACAATTTTCTTGATGATCTTGCt                                                                                    >  7:203911/1‑139 (MQ=255)
                                                  tttATAAATATGTCAAAGAATATTTTGAAATGGTGCTTACCGGGGCGAAGGAAAAGCTCTGGCGCGCGCTGGCGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGa                                                                                <  5:132072/138‑1 (MQ=255)
                                                           atGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACt                                                                      >  6:251875/1‑139 (MQ=255)
                                                                       atatTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGGCAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCag                                                            >  8:354115/1‑136 (MQ=255)
                                                                       atatTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGCTGACAATTTTCTTGATGATCTTGCTCAGATTTATGAACTGATTCAGCaa                                                            >  7:385900/1‑136 (MQ=255)
                                                                           tGCAAGGGTGCTTACCGGGGCGATGGAAACGCTCTGGCGCGCGCTGGCGGTGACGAACGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGa                                                       <  7:196721/138‑1 (MQ=255)
                                                                              aaTGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGGGCGGGCAATTTTATTGATGATCTAGCTCAGATTGATGAACTGATTCAGCATCAggtc                                                      >  4:150608/1‑134 (MQ=255)
                                                                                tGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCCCCGCCTGCTGAAAATTTTATTTATGATATTTCAAAGATTGATGAACTGAATAAGCAACAGGAccaat                                                  >  7:167786/1‑134 (MQ=255)
                                                                                  ttGCTTAAGGGGGCGACGCAACAGCGCTTGCGCGCGCTTGCGGTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCa                                               <  1:24907/138‑1 (MQ=255)
                                                                                  gTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGAttt                                                 >  8:330677/1‑137 (MQ=255)
                                                                                     tttACCGGGGCGACGTAAAGGCTGGTGCGGGGGCTTGTGCTGACGAACGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg                                            <  2:341024/138‑1 (MQ=255)
                                                                                     cttccgggggCCAAGCAAACGGTCGTGGGGGCGATTGTGCTGACGAAGGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg                                            <  7:285721/133‑1 (MQ=255)
                                                                                     cTTCACGGGGCTAAGGAAAAGCTCTGGCGCGCGCGTGAGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg                                            <  7:233217/139‑1 (MQ=255)
                                                                                     cTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGt                                             >  8:237772/1‑138 (MQ=255)
                                                                                     cTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTg                                            <  6:429419/139‑1 (MQ=255)
                                                                                     cTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATATTGCTCAGATTGATGAACTGATTAAGCAACAGGACGATTTGAGTg                                            >  5:383629/1‑139 (MQ=255)
                                                                                        acgggggCGAAGGAAAAGAGCTTGCGCGCGTTTGAGCTGACTAACGGGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa                                          <  6:383629/135‑1 (MQ=255)
                                                                                        accGGGGCGAAGGAAAAGCTCTGGCGCGCGCTTCCGCTTACGAACGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGt                                             <  4:92902/133‑1 (MQ=255)
                                                                                        accGGGGCCAAGTAAACGCTCTTGCGCGCGGTTGCGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGt                                             <  3:376002/133‑1 (MQ=255)
                                                                                        aaCGGGGCGACGGACAAGCGCTTGCGCGCGCTGGAGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa                                          <  7:208481/138‑1 (MQ=255)
                                                                                        aaCGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCAGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGaa                                          <  7:290988/138‑1 (MQ=255)
                                                                                         ccGGGGCGAAGGAAAAGCGCTGGCGCGCGCTTGAGCTGACGAACGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACt                                        <  8:388872/138‑1 (MQ=255)
                                                                                             ggCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAACGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCCGGCCGATTTCCGGGAACtata                                     >  5:383171/1‑138 (MQ=255)
                                                                                             ggCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGCCAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACtata                                     >  7:290721/1‑138 (MQ=255)
                                                                                               cGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGCTGACAAATTTAATGATGATCTTGCTGAGATTTATGAACTGATTCAGCATCAGGGCGATTTGAGTGAACtatata                                   >  7:378209/1‑138 (MQ=255)
                                                                                                gAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGGGGTGGGAATTTTCTTGATGTTCTTGCTCAGATTGTTGAACTGGTTCAGGCTGAGGGCGATTTCCGTGAAcgagat                                    >  4:144985/1‑131 (MQ=255)
                                                                                                     aaaaGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATAAGGACGATTTCAGTGAACGATATAAGCg                               >  3:92902/1‑136 (MQ=255)
                                                                                                             ttgggggcggTTGAGGGGACTAACGGGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCccgccg                    <  6:357562/133‑1 (MQ=255)
                                                                                                             ttGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCccgccg                    >  8:102654/1‑139 (MQ=255)
                                                                                                              tGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGa                                                       >  6:100210/1‑103 (MQ=255)
                                                                                                              tGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGa                                                       <  5:100210/103‑1 (MQ=255)
                                                                                                               gcgcgcgcGTGCGCGGACGACCGCGCGGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGtc                  <  1:113676/139‑1 (MQ=255)
                                                                                                                    cgcTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCAGAGAATTTTCTTGATGATCTTGCTCACTTTGAAGAACTGATTCAGCATCAGGACGAATTCAGTGAACTATATAAGCGGGTGCCCGCCGtcgggta             >  1:267450/1‑134 (MQ=255)
                                                                                                                               aCGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCa  >  2:290146/1‑139 (MQ=255)
                                                                                                                                cGAAGGCGCGGGGCGGCCGGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGc    <  4:383676/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
TGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCGAGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCGCTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACTGATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCA  >  CP006881/1103443‑1103708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A