Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F56 I13 R1
|
121 |
87.9 |
3230772 |
95.5% |
3085387 |
127.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
2,235,729 |
C→T |
E26K (GAA→AAA) |
U712_11225 ← |
Transcriptional regulatory protein resD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,235,729 | 0 | C | T | 94.1%
| 157.5
/ 1.7
| 52 | E26K (GAA→AAA) | U712_11225 | Transcriptional regulatory protein resD |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (24/24); total (27/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01 |
CATCAGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACTATGCATA > CP006881/2235595‑2235860
|
catcaGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGtca > 6:110012/1‑139 (MQ=255)
tcaGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGGTTTGtcat > 1:353773/1‑138 (MQ=255)
tcaGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGtcatc < 5:28032/139‑1 (MQ=255)
gaaaaaTTCAGTCATAATTGGCTTCCAGCCCTTTGGCAATGGCTTAATCACCATTTTCAGCATCATCTATAGCATAATTTTCACGGTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTaa < 8:314510/137‑1 (MQ=255)
aTAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTttcg < 3:185678/139‑1 (MQ=255)
tAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCTACTACTAATATTTTTGTttcgt > 5:234595/1‑139 (MQ=255)
gCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTcgttcgtt < 8:311492/138‑1 (MQ=255)
cTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTg > 7:302852/1‑139 (MQ=255)
aaGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTc > 3:143521/1‑138 (MQ=255)
aaGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTAATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTc > 6:165532/1‑138 (MQ=255)
aGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCa < 8:189922/139‑1 (MQ=255)
ttGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGtttt > 1:39122/1‑139 (MQ=255)
gCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTcc < 2:188836/138‑1 (MQ=255)
tGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTAAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTg > 6:386281/1‑139 (MQ=255)
tGGCTTCATCACCATTTTCACCTTCATCTATAGCAAAATTTTCACGTTCAACATACATTCTTAAAAGGCCGCGAATTCTGGCTTAGTCATCAACAACTAAAATTTATTTATCGTTAGTTTGGTAAATGTTATcccccag > 6:164634/1‑137 (MQ=255)
gCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGATTTCTGGCTTTGTCTTCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCt > 6:4378/1‑139 (MQ=255)
gCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTttcg > 2:71340/1‑111 (MQ=255)
gCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTttcg < 1:71340/111‑1 (MQ=255)
tcaCGATTTTCAGCTTCATCTATAGAATAATTTTCACGTTCAAGATACAATCTTAAAAGGCGGCGAATTATGTCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCtt < 3:367319/139‑1 (MQ=255)
ccATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTg < 2:39122/139‑1 (MQ=255)
ccATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTg < 5:110012/139‑1 (MQ=255)
cATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGt < 3:128893/139‑1 (MQ=255)
ttttCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGtcatc > 5:238729/1‑77 (MQ=255)
ttttCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGtcatc < 6:238729/77‑1 (MQ=255)
cATCTATAGCATAATTTTCACGTTCAAGATACATTCATAAAAGGCGTCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCTTTCGTTTGGTCCATGTTTTCCCCATGCTTTACTTCTCTTACTTTCAatt > 4:206117/1‑138 (MQ=255)
tAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTGCTGTTACTTTCATTTTATCTAATCGt > 2:292456/1‑138 (MQ=255)
tAATTTTCACGTTCAAGATACATTCTTAAAAGCCGGCGATTTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCTTGTTTTCCCGCTGCTTTCCTTCTGTTTCTTTCATTTTATCaataagt > 7:368564/1‑133 (MQ=255)
aaTTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTtcgttcgt < 1:147887/82‑1 (MQ=255)
aaTTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTtcgttcgt > 2:147887/1‑82 (MQ=255)
ttCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGaa < 8:127536/138‑1 (MQ=255)
tCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCACTGCTTTCCTTCTGTTACTTTCATTTTATAAAATCGTGAGAAAg > 4:313021/1‑139 (MQ=255)
cGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGGCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGttt < 8:258468/139‑1 (MQ=255)
cAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTgag > 7:331937/1‑138 (MQ=255)
cAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTgag > 1:224980/1‑138 (MQ=255)
cAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTgag > 3:140886/1‑138 (MQ=255)
ttCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCg > 3:199332/1‑139 (MQ=255)
tCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGc > 2:315397/1‑139 (MQ=255)
cTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGc < 6:234595/138‑1 (MQ=255)
cTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTATATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCt > 3:262382/1‑139 (MQ=255)
cTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCggt > 4:208126/1‑137 (MQ=255)
aaaaGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCAtt < 7:252385/99‑1 (MQ=255)
aaaaGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCAtt > 8:252385/1‑99 (MQ=255)
aaGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCaa < 5:165532/139‑1 (MQ=255)
aGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCaaa < 8:384333/139‑1 (MQ=255)
cggcgAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGa < 3:244017/139‑1 (MQ=255)
cggcgAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGa < 8:331937/139‑1 (MQ=255)
cggcgAATTCTGGCTTTGTCATCAACAACTAATATTTTTGTTTCCATCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGATCTGTTTCTCTTTCaaatg > 5:23112/1‑136 (MQ=255)
gcgAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACt < 1:400395/139‑1 (MQ=255)
gcgAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCATTCTGTTACTTTCATTTTATAAAATAGTGAGAAAGTTTGATAACTGTGTCGCTTTCAAAGGAc > 4:95873/1‑138 (MQ=255)
gcgAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGCTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACt < 2:14183/139‑1 (MQ=255)
ttCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACTATGCa < 4:140886/139‑1 (MQ=255)
gCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGATTATGCATa < 5:386281/136‑1 (MQ=255)
|
CATCAGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACTATGCATA > CP006881/2235595‑2235860
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
