Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F56 I0 R1
|
218 |
83.1 |
3292392 |
95.3% |
3137649 |
126.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
2,235,729 |
C→T |
18.8% |
E26K (GAA→AAA) |
U712_11225 ← |
Transcriptional regulatory protein resD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,235,729 | 0 | C | T | 18.8%
| 132.9
/ 20.9
| 59 | E26K (GAA→AAA) | U712_11225 | Transcriptional regulatory protein resD |
| Reads supporting (aligned to +/- strand): ref base C (21/26); new base T (6/5); total (28/31) |
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01 |
CATCAGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACTATGCATAAGAA > CP006881/2235595‑2235864
|
catcaGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGtca > 4:221681/1‑139 (MQ=255)
catcaGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTATTAAAATGAGGCGAATTCTGGCTTCGtc > 4:290597/1‑138 (MQ=255)
tcaGATCAAGCAATATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTGCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGtcatc < 4:367527/139‑1 (MQ=255)
tcaGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGtcatc < 1:298419/139‑1 (MQ=255)
tcaGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGtcatc < 7:28349/139‑1 (MQ=255)
gATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCCCCATTTTCCGCTTCATCTATTGCATTATTTTCACGTTCAAGATACATTCGTAAAAGGCGGCGAATTCTGGGTTCGtcatcacc > 2:245170/1‑137 (MQ=255)
gCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAActacta > 4:94360/1‑138 (MQ=255)
gCAAAATTAAGTCATAATTGGCTACAAGCCCTATGGCAATGGCTTAATCACCAGTATCAGCGTCATCTTTAGCATAATTTTCACGTTCAAGATACATTATAAAAAGGCGGCGAATTCTGGCTTAGTGATCAActacta > 7:178215/1‑138 (MQ=255)
aTTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATAttt > 8:172695/1‑139 (MQ=255)
aTAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTttcg < 4:189575/139‑1 (MQ=255)
aaTTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCCACTACTAATATTTTTGGttcgtt > 3:113619/1‑139 (MQ=255)
ggCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTtcgttcgt < 3:221681/138‑1 (MQ=255)
gCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGTCGGCGATTTCTGGCTTCGTCATAAACTACTAATATTTTTGATTCGTTCGttt > 3:249110/1‑139 (MQ=255)
gCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTcgttcgtt < 1:34130/138‑1 (MQ=255)
gCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTcgttcgtt < 5:303081/138‑1 (MQ=255)
aaGGCCTTTGGCAATGGCTTCATCACCATTATCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAATGGCGGCGAATTCTGGGTTTGTCATCATCTACTAATATTTTTGTTTCGTTCGTTTGGTc < 8:178215/138‑1 (MQ=255)
aaGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTc < 3:163058/138‑1 (MQ=255)
tGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTg > 3:325242/1‑139 (MQ=255)
tGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTg > 5:302561/1‑139 (MQ=255)
tGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTg > 3:65609/1‑139 (MQ=255)
ttttCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGtcatc < 3:22619/77‑1 (MQ=255)
ttttCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGtcatc > 4:22619/1‑77 (MQ=255)
ttttCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGtt > 4:347630/1‑138 (MQ=255)
ttttCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTa > 6:194908/1‑139 (MQ=255)
ttCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCACCTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCAtt > 8:99204/1‑139 (MQ=255)
aTCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGtcatc < 2:35807/66‑1 (MQ=255)
aTCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGtcatc > 1:35807/1‑66 (MQ=255)
cATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATct > 8:407606/1‑138 (MQ=255)
tAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATAAAATCGt > 7:127059/1‑138 (MQ=255)
tAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCa < 4:133552/124‑1 (MQ=255)
tAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCa > 3:133552/1‑124 (MQ=255)
tAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGt > 7:66350/1‑138 (MQ=255)
tAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGt > 4:398128/1‑138 (MQ=255)
tAATTTTCACGTTCAAGATACATTCTT‑AAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTAGTTTCATTTTTTAAAATCGt > 2:370334/1‑137 (MQ=255)
aaTTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGt > 3:149380/1‑137 (MQ=255)
aaTTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGt > 7:148747/1‑137 (MQ=255)
aTTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTgag < 8:42107/139‑1 (MQ=255)
ttCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCTTCATCAACTCCTAATATTTTTGTATCGTTCGTTTGGTCCTTGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGaa < 2:30559/138‑1 (MQ=255)
ttCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGaa < 2:30558/138‑1 (MQ=255)
tCAAGATACATTCATAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTgag < 6:300120/139‑1 (MQ=255)
cAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTgag < 2:235683/138‑1 (MQ=255)
aTTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTc < 3:94360/139‑1 (MQ=255)
cTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGc < 4:65609/138‑1 (MQ=255)
aaaGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCa < 3:398128/139‑1 (MQ=255)
aaaGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATAATTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCATTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTc > 2:37251/1‑138 (MQ=255)
cggcgAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGa < 5:194908/139‑1 (MQ=255)
cggcgAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGa < 3:347630/139‑1 (MQ=255)
cggcgAATTCTGGCTTTGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCGTTCCTTCTGTTTCTTTCATTTTATCAAATCGTGAGAAAGTTTGAGACCTGTGTCGCTTTCAAAGGa > 6:118530/1‑139 (MQ=255)
cggcgAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGa < 1:94870/139‑1 (MQ=255)
cggcgAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGa > 7:81208/1‑139 (MQ=255)
cggcgAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGa < 8:148747/139‑1 (MQ=255)
cggcgAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGa < 8:66350/139‑1 (MQ=255)
ggcgAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTGCGTTCGTTTGGTCGATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGAc < 3:403609/139‑1 (MQ=255)
gcgAATTCTTGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACt < 3:370880/139‑1 (MQ=255)
gcgAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACt > 3:332207/1‑139 (MQ=255)
gCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACTATGCATa < 3:290597/136‑1 (MQ=255)
gCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACTATGCATa < 4:325242/136‑1 (MQ=255)
cTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAATTACTATGCATAAGaa < 4:167682/139‑1 (MQ=255)
cTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACTATGCATAAGaa < 6:302561/139‑1 (MQ=255)
|
CATCAGATCAAGCAAAATTAAGTCATAATTGGCTTCAAGCCCTTTGGCAATGGCTTCATCACCATTTTCAGCTTCATCTATAGCATAATTTTCACGTTCAAGATACATTCTTAAAAGGCGGCGAATTCTGGCTTCGTCATCAACTACTAATATTTTTGTTTCGTTCGTTTGGTCCATGTTTTCCCCCTGCTTTCCTTCTGTTACTTTCATTTTATCAAATCGTGAGAAAGTTTGAGAACTGTGTCGCTTTCAAAGGACTATGCATAAGAA > CP006881/2235595‑2235864
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
