Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A27 F74 I1 R1
|
23 |
45.7 |
1836232 |
90.6% |
1663626 |
141.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
460,772 |
T→G |
S629A (TCG→GCG) |
lon → |
Lon protease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 460,772 | 0 | T | G | 96.7%
| 77.2
/ NA
| 30 | S629A (TCG→GCG) | lon | Lon protease |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (16/13); minor base A (1/0); total (17/13) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGA > NC_000913/460633‑460904
|
gCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTAAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACAGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACAGGTACGc > 2:507895/1‑143 (MQ=255)
tCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTACAGGCAAGCTGACCTATACCGGTGGGCTCGGCGAAGtgctgc > 2:134699/1‑143 (MQ=255)
aggATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAg > 2:609997/2‑143 (MQ=255)
tAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCa < 1:395818/143‑1 (MQ=255)
cGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAg > 2:627276/1‑133 (MQ=255)
cGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAg < 1:627276/133‑1 (MQ=255)
gAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACCGACCTATACCGGTGCGCTCGGCGAAGTGAGGCAGGAGTCCATTCa > 2:786818/1‑143 (MQ=255)
aaCCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGGGAAGTGATGCAGGAGTCCATTGAggc > 2:230073/1‑143 (MQ=255)
cgggtCGGTCAGGTGCGCGGTCTGGCGTGGGCGGAAGTGGGCGGTGACTTGCTGACCATTGAATCCGCAGGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAggcggc < 2:167915/140‑1 (MQ=255)
gTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGCTGCAGGAGTCCATTCAGGCGGCGTTAACggtggt > 2:810290/1‑143 (MQ=255)
tGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCACTGGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCAGCCGGGCGGCGCGAACGGTGGTTCGTGCGCGGGCGGa > 2:748067/1‑143 (MQ=255)
gACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGGCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGaaa > 1:169736/1‑143 (MQ=255)
tggaGTGGGCGGTGACTTGCTGAGCGTGTGGACCGGGGGGGTCGCGGTTAAATGCAAACTGTCCTATACCGGTGCGCCCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGAGTTAACGGTGGTTCGTGTGCGTGCGGAAAAAc < 2:150167/140‑1 (MQ=255)
aaGTGGGCGGTGACTTGCTTACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTg < 1:134699/143‑1 (MQ=255)
gTGACTTGCTGACCATTGAACCCGCCTGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCCCGGCGACGTGATGCACGAGTCCATTCAGGCGGCGTTCACGGTGGTCCGTGCGCGCGCGCGAAAACCGCGGATCGAc > 2:60609/1‑143 (MQ=255)
tGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCAATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAGAGACTGGGGGTCAAcc > 1:817923/1‑143 (MQ=255)
ttGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAGAACTGGGAAGGCACCCTGa > 2:249797/1‑143 (MQ=255)
cATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACg > 1:599357/1‑143 (MQ=255)
cATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTCCGTGCGCGTGCGGCACAACTGGGGCTGAACCCTGATTTGTACg > 1:358220/1‑143 (MQ=255)
aaaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCCGAGCGCTCGGCGGCGTGATGCAGGACACCATTCAGGCCGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGCGTCAACCCGGATTTTTAAGaacca > 2:458905/1‑140 (MQ=255)
ccGCAGGGGTTGCGGGTAAAGGCAAACCGACCGACACCGGTGCGGGCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGATAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 2:336079/143‑1 (MQ=255)
gggTAAATGCAACCTGACCTATACCGGTGCGCGCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtcc < 1:846467/143‑1 (MQ=255)
ggTAAAGGCAAACTGACCTCTCCCGGTGCGCTCGGCCAAGTGCTGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtcca < 1:796759/143‑1 (MQ=255)
aGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAc < 2:357798/143‑1 (MQ=255)
gCAAACTGACCTATCCCGGTGCGCTCGGCGAAGTGATGCAGGAGAACGTTCCCTCCCCGTGAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCg < 1:208113/143‑1 (MQ=255)
aaaCTGATCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGA‑TTTTACGAAAAACGTGACATCCACGTCCACGTACCGGaa < 1:291690/143‑1 (MQ=255)
aCTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGGCCATTCAGGCGGCGCTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGAGCAACCCTGATTTTTACGAAGAACGTGACATCCACGAGCGCGTACCGGagg > 1:588897/1‑141 (MQ=255)
ccgACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAgg < 1:786818/141‑1 (MQ=255)
gACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTg < 1:507895/143‑1 (MQ=255)
cTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAGCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGCCAAACGTGACATCCACGTCGACGTACCGCGAGGTGCGa > 1:813187/1‑143 (MQ=255)
|
GCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGA > NC_000913/460633‑460904
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
