Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A27 F124 I0 R1
|
44 |
29.6 |
1159012 |
90.6% |
1050064 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
460,772 |
T→G |
100% |
S629A (TCG→GCG) |
lon → |
Lon protease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 460,772 | 0 | T | G | 100.0%
| 45.9
/ NA
| 16 | S629A (TCG→GCG) | lon | Lon protease |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (6/10); total (6/10) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCC > NC_000913/460634‑460908
|
ggTTTCGTCTATGGTCGCGCGGGGAACGGAAAGCGTTTCGGTCAGGTAGCCGGTCTGGCGTGGTCGGATGTGGGCGGTGACTTGCTGACGATTGAAACCGCATGTCTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCt < 2:447880/142‑1 (MQ=255)
tGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACCGACCGATACCGGTGCGCTCGGCGAAGCGa > 2:114617/1‑143 (MQ=255)
cgcgcgGATAACGAAGGCCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCa < 1:251214/143‑1 (MQ=255)
ccGTGGCGGTCAGGGAGCCGGTCTGGCGTGGCCGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTCCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAggcgg < 1:200885/143‑1 (MQ=255)
aGGTAACCGCTCGGGCGTGGACGGGAGTGCGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGCGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACggtg < 1:462397/143‑1 (MQ=255)
gTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCGCGGCGAAGTGACGCAGGAGTCCATTCAGGCGGCGGTAACggtggt > 2:491103/1‑143 (MQ=255)
aaGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTg < 1:310249/143‑1 (MQ=255)
tGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTAGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTgggg > 2:18507/1‑143 (MQ=255)
gCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATc > 1:569432/1‑143 (MQ=255)
gTGACTTGCTGACCATTGACACCGCCGTTCTTCCCGGTAAATCCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTACATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAAc < 1:160393/143‑1 (MQ=255)
tGACACCGCATGTGTGCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGaaa < 1:230332/143‑1 (MQ=255)
aTGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGCGATCAACCCTGATTTTTACGAAAAACGTGACa > 2:521214/1‑143 (MQ=255)
ggCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAcc > 1:575234/1‑143 (MQ=255)
tGACCTATACCGGTGCGCGCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGt < 1:69767/143‑1 (MQ=255)
ctccaCCGGGGCGCGCCGCGACGCGCTGCAGGCGCCCTTCCAGGCGGCGTGAACGGTGGTTCGTGCGCGTGCGGACAAACTGGGGATCAACCCTGATTGTTACGAAAGACGTGACATCCACGTCCACGTACCGGAAGGTGCGa < 2:171274/139‑1 (MQ=255)
aCCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGcc < 2:569432/143‑1 (MQ=255)
|
CGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCC > NC_000913/460634‑460908
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
