Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A27 F124 I1 R1	28	40.1	1601150	89.4%	1431428	142.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	460,772	T→G	S629A (TCG→GCG)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	460,772	0	T	G	100.0%	114.9 / NA	41	S629A (TCG→GCG)	lon	Lon protease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/24); total (17/24)

GCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGA  >  NC_000913/460633‑460910

gCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGGAGAGGCAAACTGACCTGTACCGGTgcgc                                                                                                                                         >  1:596296/1‑143 (MQ=255)

        actagcgTCTCGCTGATAACGAACACCGGGGCGGTCAGGTAGCCGGTCTGGCGTGGTGGGATGTGGGCGCTGACTCGCGAACCATTGAAACCGCTTGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGaa                                                                                                                                 <  2:603746/137‑1 (MQ=255)

              gTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGGGTTCCGGGTAGAGGCAAAGTCACCTATACCGGGGCGCGCGGCGACgtggtg                                                                                                                           >  2:330213/1‑143 (MQ=255)

              gTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTCGCTGACCATTGAAACCGCATGTGTTCCGGGTACAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATg                                                                                                                           >  2:615689/1‑143 (MQ=255)

               tCGCGCGGATAACGAAAACCGTGTCGGTCAGGTACCCGGTCTGGCGTGGACGGAAGTGGGAGGTGACTTGCTGACCATTGAAACCGCGAGTGTTCTGGGTAAAGGCAGACTGACCTATACCGGTGCGCTCGGCGAAGTGATGc                                                                                                                          >  1:129497/1‑143 (MQ=255)

                   gcgGATACCGGAAACCTTGCCGGTCAGGTAACCGGTCTGGCGTGGGCGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAAATGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGa                                                                                                                      <  2:265345/143‑1 (MQ=255)

                     ggATGACGAAAGCCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGt                                                                                                                    <  1:164146/143‑1 (MQ=255)

                                gcccggtCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATGCAggcg                                                                                                         <  2:323318/138‑1 (MQ=255)

                                           cAGGTAACCGGGCCGGCGGGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACggt                                                                                              <  1:597341/143‑1 (MQ=255)

                                                acccGGTCGGGCGAGCACGGAACGGGGCGGTGACTTGCTGACCCTTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGCTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTc                                                                                         <  1:91868/141‑1 (MQ=255)

                                                    ggTCTGGCGTTGACTGGTGCGGGCGGCGACGTGCCGACCATGGAAACCCCATGGGTTCCGGGTAAAAGCCAGCGGCCCTATACGGGTGCGCTGGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgc                                                                                     <  2:401422/143‑1 (MQ=255)

                                                             tGGACGGAAGCGGGCGGTTGCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATCCCGGTGCGCTCGGCGAAGTGATGCCGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGa                                                                            <  1:100278/143‑1 (MQ=255)

                                                                 cGGGAGTGGGTGGTGCCTTGCTTACCATGGGAGCCGGATGGGGTGCGTGTAAAGGCAAACTGCCCTATACCGGTGCGCTCGGCGAAGTGACGCAGGAGGCCATTCAGGCGGCGTTAACGGTGGGTCGTGCGCGGGCGGaaaaa                                                                        <  2:163049/143‑1 (MQ=255)

                                                                  ggAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTCTACCGGTGCGCTCGGCCAAGGGACGCCGGAGGCCGTTCAGGCGGCGTGAACGGTGCTTCGTGCGCGTGCggccatcc                                                                       >  1:150578/1‑137 (MQ=255)

                                                                     ggTGGGCGGTGACTTGCGGACCAGTGGGAGCGCATGCGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTTGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTgg                                                                    <  2:235386/142‑1 (MQ=255)

                                                                     aGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTgg                                                                    <  1:765472/143‑1 (MQ=255)

                                                                     aGTGGGCGGTCACGTGCTGACTACTGAGATGGCAGGGGTGCCGGGTAAATGCAAAGTGACCTATACGGGTGCGCGCGGCGAGGTGATGCAGGAGTCCAGTCAGGTGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTgg                                                                    <  2:786519/143‑1 (MQ=255)

                                                                      gTGGGCGGTGACTTTCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCTAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTggg                                                                   <  1:511748/143‑1 (MQ=255)

                                                                       tgctcGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTgggg                                                                  <  1:137909/139‑1 (MQ=255)

                                                                        gggCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAATCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGACAAACTagggc                                                                 >  2:38949/1‑142 (MQ=255)

                                                                            ggTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGAACaa                                                             >  2:71738/1‑143 (MQ=255)

                                                                                     cTGACCATTGAAACCGCATGTCTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCCGCGTTAACGGTGGTTCGTGCGCGTGCGGAAACGCTGGGGATCAACCCTgcgtt                                                    >  2:285876/1‑139 (MQ=255)

                                                                                      tGACCAGTGAAACCGCATGGGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGGCCATTCAGGCGGCGTTAACGGTGGTGCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGAtttt                                                   <  1:150713/143‑1 (MQ=255)

                                                                                        aCCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTa                                                 <  1:381537/143‑1 (MQ=255)

                                                                                        aCCATTGAAACCGCATGTGTTCCGGGTAAAGGAAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTa                                                 <  1:308509/143‑1 (MQ=255)

                                                                                        aCCATCGGAACCGCATGTGGTCCGGGTAAAGGCAAACTGACCTATACCGGCGCGCTCGGCGAAGGGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTa                                                 <  1:631672/143‑1 (MQ=255)

                                                                                          cATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAGAATGGGGATCAACCCTGAtgtcgccg                                               >  2:656397/1‑136 (MQ=255)

                                                                                           aTTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGTTAAACTGGGGATCAACCCTGATTTTTACGa                                              >  1:146172/1‑143 (MQ=255)

                                                                                           aTTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGa                                              >  1:72278/1‑143 (MQ=255)

                                                                                               aacccGCATGTGTTCCGGGTAAAGGCAAACTGACCTATCCCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGaaaaa                                          <  1:31911/140‑1 (MQ=255)

                                                                                                caCCCCATCTGCTCCGGGTAAAGGCACACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCTTCCGCGTGCGGAAAAACTGGGTATCAACCCTGATTTTTACGAAAAAc                                         <  1:332948/142‑1 (MQ=255)

                                                                                                         tgtTTCGGGTAAAGTTTATCTGTCATATACCGGTGCCAACGGCGAAGGGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcc                                <  1:328628/143‑1 (MQ=255)

                                                                                                           ttCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccac                              >  2:362061/1‑143 (MQ=255)

                                                                                                              cGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtc                           >  2:424718/1‑143 (MQ=255)

                                                                                                              cGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGGCAACAGTGGTTCGTGCGAGCCCCGAAAGACGGGGGATCAACCCTGATTTTTACGAAAAGCGGGACAtccacggc                           >  2:432513/1‑141 (MQ=255)

                                                                                                                  tAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGTGTTTAGGGTGGTCCGAGCAGGTGAGAACATAACGCAGATCAACCCTGATTTCTACGCACAACCCGACAgccacgtccacg                       >  2:565825/1‑143 (MQ=255)

                                                                                                                     aGGCGAACTGTCCTATCCCGGTGCGCCTGGCGAAGTGATGCAGGAAATAATGCTTGCGACATTCAAAGTTTTGCTGGCTAGTGCGGAAAACCTGGGGATCTCCACTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAc                    <  2:565766/143‑1 (MQ=255)

                                                                                                                       gCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGCCCATTCAGGCGGCGTCAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGAGCAACCCTGCTTTTTCCGCAACACGTGACATCCACGCCCACGTACCg                  >  2:113633/1‑143 (MQ=255)

                                                                                                                            cTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAgg             <  1:71738/143‑1 (MQ=255)

                                                                                                                             tGACCTAGTCCGGTGCGCTCCGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGt            <  2:445608/143‑1 (MQ=255)

                                                                                                                                       cGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGGTTTTTACGAAAAACGTGACATCCACGTCCGCGTACCGGAAGGTGCGACGCCgg  >  1:504859/1‑142 (MQ=255)

                                                                                                                                       cGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATCCAGGCGGCGTTAACGGTGGTCCGGGCGCGTGCGGAACAACTGGGGATCAACCCTGATTGCTCCGAACAACGTGACCTCCCCGTCCACGTGCCGGCAGGTGCGACGCCGa  >  2:399215/1‑143 (MQ=255)

GCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGA  >  NC_000913/460633‑460910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A