breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 124,241 C→T Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
RA 136,457 (T)8→7 coding (8/867 nt) speE ← spermidine synthase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 460,772 T→G S629A (TCG→GCG)  lon → Lon protease
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,405,158 (A)8→7 intergenic (‑86/+545) nuoA ← / ← lrhA NADH:quinone oxidoreductase subunit A/DNA‑binding transcriptional dual regulator LrhA
RA 2,661,821 C→G C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,634 A→C T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,310,841 C→T E110K (GAA→AAA)  pnp ← polynucleotide phosphorylase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp [rph] [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423748–3424541 3424541 1–794 19 [18] [18] 19 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 315852NA (NA)29 (0.610) 15/272 2.5 NA noncoding (624/1255 nt) IS3 repeat region
?NC_000913 = 315875 NA (NA)noncoding (647/1255 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)3587 (73.530) 276/278 0.0 98.1% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 71 (1.450)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein