Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A21 F97 I0 R1
|
51 |
42.6 |
1617048 |
90.7% |
1466662 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
454,891 |
G→T |
100% |
intergenic (+102/‑242) |
bolA → / → tig |
DNA‑binding transcriptional dual regulator BolA/trigger factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 454,891 | 0 | G | T | 100.0%
| 85.6
/ NA
| 32 | intergenic (+102/‑242) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/18); total (14/18) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAC > NC_000913/454752‑455028
|
ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTccc > 2:364909/1‑143 (MQ=255)
tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGACCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGAc < 1:769813/143‑1 (MQ=255)
gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGAGTTTATGAAAAACGGCCTGCGGGCCGTTCTGCTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATCCCGACggcc > 1:122110/1‑140 (MQ=255)
gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTGTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAACTTGc < 1:681715/143‑1 (MQ=255)
aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCggg < 1:251816/143‑1 (MQ=255)
cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt < 1:364909/143‑1 (MQ=255)
tAAGAAGGCATTTGCAACTGTCGGCGCTTTTCCGGTTTGTTGCTAAAGATTTTATGAGAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATGTTGCGCTTTTCTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa < 2:23537/143‑1 (MQ=255)
tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAGACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa < 2:209043/143‑1 (MQ=255)
cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc < 1:554896/143‑1 (MQ=255)
tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGa > 1:140034/1‑143 (MQ=255)
tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTCTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAGTTGCCCGGGAATTGTCAAAAAATACGCGa > 2:264232/1‑143 (MQ=255)
aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcg > 2:355552/1‑143 (MQ=255)
gcgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCACAAAATAATGTTGAAGAGTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa > 1:182847/1‑143 (MQ=255)
gcTTTTCCGTTATGTTGCTAAAGCTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTGTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc < 2:626703/143‑1 (MQ=255)
tttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt < 1:686999/143‑1 (MQ=255)
ttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCACTAACCGTtc > 2:143990/1‑143 (MQ=255)
cttATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa < 1:785882/141‑1 (MQ=255)
tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCCTAAAAg > 1:567467/1‑143 (MQ=255)
aaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTATCGACTCATAAAAGTg > 2:329884/1‑143 (MQ=255)
aaaCGGCGGGCGGGCCGTTTTGTTGTGTCGGGATTTTGCGCTTTTTTCCCAGCATGCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt < 1:277243/143‑1 (MQ=255)
cGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc < 1:527910/143‑1 (MQ=255)
cGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc < 1:715942/143‑1 (MQ=255)
cGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGGAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATCAAAGTGATGCCGCCATAATGCCGCGTCCt > 2:756512/1‑143 (MQ=255)
tttgtttggtCTGGTTTTTGCGCTTTTTTTCGAGCATCCCGACCAAAATTGGCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGATTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAAt < 2:318227/143‑1 (MQ=255)
gttttgtCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt > 1:787003/1‑143 (MQ=255)
gttttgtCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt > 2:460577/1‑143 (MQ=255)
tgtCTGGATTTTGCGCTTCTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCg < 1:78270/143‑1 (MQ=255)
tgtCTGGATGTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAACGCGCAATACCCGTTCTCTTCTCTTATAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCg < 1:462517/143‑1 (MQ=255)
tGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCCCTATAATGCTGCGTCCTATTTGAATGCTTTCGGgat > 1:546583/1‑143 (MQ=255)
ggATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTTGGgttg > 1:755509/1‑143 (MQ=255)
ttGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt < 1:165606/143‑1 (MQ=255)
gcgcTTTTTTCCCAGCATTCAGACGAAACTTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTgg < 2:47873/143‑1 (MQ=255)
ttttttCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAc < 1:62703/143‑1 (MQ=255)
|
CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAC > NC_000913/454752‑455028
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
