Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F61 I1 R1	18	150.6	5884618	93.9%	5525656	127.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,158,703	A→G	T100A (ACA→GCA)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,158,703	0	A	G	97.7%	451.7 / ‑0.9	131	T100A (ACA→GCA)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (65/63); total (66/65)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 135 total aligned reads displayed.

ACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTG  >  NC_000913_3_pae_tpiA/4158556‑4158849

   aGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCACGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGtt                                                                                                                                               <  4:48218/150‑1 (MQ=255)

     ccGTAAAGTGTTGTTCACCCAGGCGGGAATGTTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTgg                                                                                                                                             >  2:59415/1‑150 (MQ=255)

      cGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGa                                                                                                                                            >  3:311179/1‑150 (MQ=255)

      cGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGa                                                                                                                                            >  2:365355/1‑150 (MQ=255)

         aaaGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCg                                                                                                                                         >  3:505379/1‑150 (MQ=255)

           aGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTa                                                                                                                                       >  2:1806413/1‑150 (MQ=255)

             tgttgtTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTAcc                                                                                                                                     <  4:658710/150‑1 (MQ=255)

               ttgttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTg                                                                                                                                   >  2:58479/1‑150 (MQ=255)

               ttgttCACCCAGGCGGGAATGCGGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGCAAACGTCCTTAAAGAGATGGGAAGCCACCAGGGCGAGACGATGTCCGGACCGCGGCACCTTGGGTTGATTCCCGCGGTTGGACCGTCCCTg                                                                                                                                   >  2:1391605/1‑150 (MQ=255)

                 gttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAAGACTTGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCt                                                                                                                                 >  1:495402/1‑150 (MQ=255)

                 gttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGCGAGGTGAACGTCCTTAAAGAGCTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGcg                                                                                                                                 >  4:365411/1‑149 (MQ=255)

                      cccAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGc                                                                                                                            >  1:648280/1‑150 (MQ=255)

                         aGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCata                                                                                                                         <  4:311179/150‑1 (MQ=255)

                         aGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCata                                                                                                                         >  4:246800/1‑150 (MQ=255)

                                aaTGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATccc                                                                                                                  >  2:1513804/1‑150 (MQ=255)

                                 aTGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCt                                                                                                                 >  1:167805/1‑150 (MQ=255)

                                   gctgctGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAt                                                                                                               <  2:310675/150‑1 (MQ=255)

                                            ggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCa                                                                                                      >  1:721475/1‑150 (MQ=255)

                                             gATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAg                                                                                                     >  4:825076/1‑150 (MQ=255)

                                               tCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCcgca                                                                                                                                                  <  3:213633/103‑1 (MQ=255)

                                               tCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCcgca                                                                                                                                                  >  4:213633/1‑103 (MQ=255)

                                               tCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctgc                                                                                                          >  4:83705/1‑143 (MQ=255)

                                               tCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctgc                                                                                                          <  3:83705/143‑1 (MQ=255)

                                                 aGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCt                                                                                                 <  2:232722/150‑1 (MQ=255)

                                                     gcgTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTcc                                                                                             >  2:1409125/1‑150 (MQ=255)

                                                       gTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCaa                                                                                           <  1:490759/150‑1 (MQ=255)

                                                         aCCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTa                                                                                                                                >  1:745660/1‑111 (MQ=255)

                                                          ccGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGc                                                                                        >  1:1415199/1‑150 (MQ=255)

                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCt                                                                                                                                    <  1:1739823/105‑1 (MQ=255)

                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCt                                                                                                                                    >  2:1739823/1‑105 (MQ=255)

                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                   >  1:232667/1‑122 (MQ=255)

                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                   <  2:232667/122‑1 (MQ=255)

                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                       <  3:825076/150‑1 (MQ=255)

                                                                   gTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaaa                                                                                  <  2:256819/147‑1 (MQ=255)

                                                                   gTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaaa                                                                                  >  1:256819/1‑147 (MQ=255)

                                                                       ggTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGAc                                                                                                                                           <  2:1174518/86‑1 (MQ=255)

                                                                       ggTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGAc                                                                                                                                           >  1:1174518/1‑86 (MQ=255)

                                                                       ggTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCt                                                                           <  3:660930/150‑1 (MQ=255)

                                                                        gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTaacgt                                                                          >  2:374273/1‑146 (MQ=255)

                                                                        gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTg                                                                          >  3:1021750/1‑150 (MQ=255)

                                                                           aaaGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                       <  1:1806413/150‑1 (MQ=255)

                                                                           aaaGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                       <  1:1409125/150‑1 (MQ=255)

                                                                              gTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGaa                                                                    <  1:1513804/150‑1 (MQ=255)

                                                                               tCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAg                                                                   <  2:1415199/150‑1 (MQ=255)

                                                                               tCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTAACCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAg                                                                   <  1:59415/150‑1 (MQ=255)

                                                                               tCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAg                                                                   <  2:721475/150‑1 (MQ=255)

                                                                                 cTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGa                                                                                                    >  2:1615291/1‑115 (MQ=255)

                                                                                 cTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGa                                                                                                    <  1:1615291/115‑1 (MQ=255)

                                                                                  ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                                                                <  2:1301662/150‑1 (MQ=255)

                                                                                    aaaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAg                                                              >  1:1671543/1‑150 (MQ=255)

                                                                                     aaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAcc                                                                                                       >  4:809804/1‑108 (MQ=255)

                                                                                     aaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAcc                                                                                                       <  3:809804/108‑1 (MQ=255)

                                                                                     aaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGa                                                             <  2:495402/150‑1 (MQ=255)

                                                                                      agagATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAc                                                            >  1:1044801/1‑150 (MQ=255)

                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTgg                                                                                     >  2:1805041/1‑119 (MQ=255)

                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccacca                                                      >  2:102960/1‑150 (MQ=255)

                                                                                             gCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAg                                                     >  2:1536855/1‑150 (MQ=255)

                                                                                              cAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                               >  1:162088/1‑107 (MQ=255)

                                                                                              cAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                               <  2:162088/107‑1 (MQ=255)

                                                                                                  ccAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCa                                                                                                                           <  4:1082750/75‑1 (MQ=255)

                                                                                                  ccAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCa                                                                                                                           >  3:1082750/1‑75 (MQ=255)

                                                                                                   cagcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                       <  3:678197/126‑1 (MQ=255)

                                                                                                   cagcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                       >  4:678197/1‑126 (MQ=255)

                                                                                                    agcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTgg                                              >  1:954684/1‑150 (MQ=255)

                                                                                                     gcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGgc                                             >  1:1365751/1‑150 (MQ=255)

                                                                                                      cagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCt                                                                           <  3:152149/119‑1 (MQ=255)

                                                                                                          gCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                               <  1:301449/95‑1 (MQ=255)

                                                                                                            gagaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTg                                      <  4:255598/150‑1 (MQ=255)

                                                                                                            gagaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTg                                      <  4:1021750/150‑1 (MQ=255)

                                                                                                               aCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGAc                                   >  3:504778/1‑150 (MQ=255)

                                                                                                                  aTGTCCGGACCGCTGCACATTGGTTTGATTCCcgca                                                                                                                                                  <  2:402103/36‑1 (MQ=255)

                                                                                                                      ccGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                              >  4:132920/1‑84 (MQ=255)

                                                                                                                      ccGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                              <  3:132920/84‑1 (MQ=255)

                                                                                                                       cGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGc                                                                                                                            <  2:33267/53‑1 (MQ=255)

                                                                                                                            cGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCg                      >  1:1549345/1‑150 (MQ=255)

                                                                                                                             gCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtg                                                                                                              <  1:228826/61‑1 (MQ=255)

                                                                                                                              cTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTg                                      <  3:528127/132‑1 (MQ=255)

                                                                                                                              cTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTg                                      >  4:528127/1‑132 (MQ=255)

                                                                                                                              cTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGAt                    >  2:671571/1‑150 (MQ=255)

                                                                                                                               tGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGAtt                   <  3:60991/150‑1 (MQ=255)

                                                                                                                               tGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGAtt                   <  4:504778/150‑1 (MQ=255)

                                                                                                                                gCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                   >  3:403240/1‑53 (MQ=255)

                                                                                                                                gCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTg                  >  3:878921/1‑150 (MQ=255)

                                                                                                                                 cacaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGc                 <  4:392238/150‑1 (MQ=255)

                                                                                                                                  acaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTAACAGATTGTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCg                <  1:479884/150‑1 (MQ=255)

                                                                                                                                   caTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGt               <  3:175950/150‑1 (MQ=255)

                                                                                                                                    aTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCGACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTg              <  2:1246642/150‑1 (MQ=255)

                                                                                                                                    aTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTg              >  1:958683/1‑150 (MQ=255)

                                                                                                                                    aTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTg              >  2:1658404/1‑150 (MQ=255)

                                                                                                                                     ttGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                                    <  4:935406/127‑1 (MQ=255)

                                                                                                                                        gTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                               >  3:84381/1‑65 (MQ=255)

                                                                                                                                        gTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                               <  4:84381/65‑1 (MQ=255)

                                                                                                                                         tttGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                       >  2:600755/1‑88 (MQ=255)

                                                                                                                                         tttGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                       <  1:600755/88‑1 (MQ=255)

                                                                                                                                         tttGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCt         >  3:415120/1‑150 (MQ=255)

                                                                                                                                            gATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTcgc      <  1:1508471/150‑1 (MQ=255)

                                                                                                                                             aTTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAc                                                            <  1:1073775/95‑1 (MQ=255)

                                                                                                                                             aTTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTcgcg     >  1:754285/1‑150 (MQ=255)

                                                                                                                                             aTTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTc        >  4:44879/1‑147 (MQ=255)

                                                                                                                                                cccGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCGGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTg  <  1:1479960/150‑1 (MQ=255)

ACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTG  >  NC_000913_3_pae_tpiA/4158556‑4158849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A