Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F141 I1 R1	21	114.5	4481172	94.2%	4221264	126.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,158,703	A→G	T100A (ACA→GCA)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,158,703	0	A	G	94.4%	280.6 / 5.2	90	T100A (ACA→GCA)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (3/2); new base G (41/44); total (44/46)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

ACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCG  >  NC_000913_3_pae_tpiA/4158556‑4158846

aCCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGACAGTCCTTAAAGAGATGTCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCcaca                                                                                                                                               <  4:647045/150‑1 (MQ=255)

 ccAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAg                                                                                                                                              <  3:182949/150‑1 (MQ=255)

   aGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGtt                                                                                                                                            >  4:367735/1‑150 (MQ=255)

    gCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTg                                                                                                                                           >  4:416705/1‑150 (MQ=255)

      cGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGa                                                                                                                                         <  3:100776/150‑1 (MQ=255)

       gTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGAc                                                                                                                                        <  2:109643/150‑1 (MQ=255)

       gTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGAc                                                                                                                                        >  4:639573/1‑150 (MQ=255)

        tAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGAc                                                                                                                                        <  3:667977/149‑1 (MQ=255)

        tAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGAc                                                                                                                                        >  4:667977/1‑149 (MQ=255)

              gttgttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCCGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCt                                                                                                                                 <  1:745195/150‑1 (MQ=255)

                    cACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTAcc                                                                                                                           >  2:1031927/1‑150 (MQ=255)

                         aGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCata                                                                                                                      <  4:445852/150‑1 (MQ=255)

                           gCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCAtatt                                                                                                                    >  2:29515/1‑150 (MQ=255)

                               gAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                <  3:416705/150‑1 (MQ=255)

                                   gctgctGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAt                                                                                                            <  2:7545/150‑1 (MQ=255)

                                   gctgctGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAt                                                                                                            <  2:1107490/150‑1 (MQ=255)

                                     tgctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGAcc                                                                                                                                       <  2:641546/121‑1 (MQ=255)

                                     tgctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGAcc                                                                                                                                       >  1:641546/1‑121 (MQ=255)

                                         ggtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCa                                                                                                      <  4:49235/150‑1 (MQ=255)

                                          gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCat                                                                                                                       <  2:114124/132‑1 (MQ=255)

                                          gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCat                                                                                                                       >  1:114124/1‑132 (MQ=255)

                                             gATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGAGCTCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAg                                                                                                  >  3:640566/1‑150 (MQ=255)

                                                   gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                            <  1:1031927/150‑1 (MQ=255)

                                                   gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                            <  1:29515/150‑1 (MQ=255)

                                                     gcgTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCata                                                                                                                      >  3:50489/1‑122 (MQ=255)

                                                     gcgTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCata                                                                                                                      <  4:50489/122‑1 (MQ=255)

                                                         aCCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAg                                                                                      <  2:1285481/150‑1 (MQ=255)

                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCAtatt                                                                                                                    <  4:202584/118‑1 (MQ=255)

                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCAtatt                                                                                                                    >  3:202584/1‑118 (MQ=255)

                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                    >  1:1307176/1‑150 (MQ=255)

                                                             tgctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTgg                                                                                  >  3:497686/1‑150 (MQ=255)

                                                               ctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACGTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaa                                                                                <  2:954879/150‑1 (MQ=255)

                                                                 gcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAAt                                                                              <  3:639573/150‑1 (MQ=255)

                                                                     gAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                          >  4:365506/1‑150 (MQ=255)

                                                                       ggTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAtgc                                                                        >  3:545508/1‑148 (MQ=255)

                                                                        gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCg                                                                                                                          >  2:531811/1‑99 (MQ=255)

                                                                        gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCg                                                                                                                          <  1:531811/99‑1 (MQ=255)

                                                                        gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                           >  4:644608/1‑130 (MQ=255)

                                                                        gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                           <  3:644608/130‑1 (MQ=255)

                                                                        gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATg                                                                             <  1:650041/144‑1 (MQ=255)

                                                                        gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATg                                                                             >  2:650041/1‑144 (MQ=255)

                                                                          gAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGc                                                                                     <  2:1307030/134‑1 (MQ=255)

                                                                          gAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGc                                                                                     >  1:1307030/1‑134 (MQ=255)

                                                                          gAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCa                                                                     <  2:1307176/150‑1 (MQ=255)

                                                                          gAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCa                                                                     >  2:977327/1‑150 (MQ=255)

                                                                              gTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGaa                                                                 >  1:1104371/1‑150 (MQ=255)

                                                                                  ttAAAGAGATGGCTAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                <  2:985491/99‑1 (MQ=255)

                                                                                  ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                >  1:985491/1‑99 (MQ=255)

                                                                                  ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                                                             >  2:615527/1‑150 (MQ=255)

                                                                                   tAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcaca                                                            <  1:615527/150‑1 (MQ=255)

                                                                                    aaaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAg                                                           <  3:593702/150‑1 (MQ=255)

                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAt                                                                                                            >  1:282641/1‑93 (MQ=255)

                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAt                                                                                                            <  2:282641/93‑1 (MQ=255)

                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGa                                                                                                 >  1:166376/1‑104 (MQ=255)

                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGa                                                                                                 <  2:166376/104‑1 (MQ=255)

                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccacca                                                   >  2:1180299/1‑150 (MQ=255)

                                                                                             gCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGa                                                                                 <  1:825274/119‑1 (MQ=255)

                                                                                             gCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGa                                                                                 >  2:825274/1‑119 (MQ=255)

                                                                                             gCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAg                                                  >  2:404162/1‑150 (MQ=255)

                                                                                             gCAAGCCAGAAGGGCGAGCCGATGTCCGGACCGCTGAACATTGGATTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAg                                                  >  2:269943/1‑150 (MQ=255)

                                                                                              cAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGt                                                 >  2:765463/1‑150 (MQ=255)

                                                                                                aGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTa                                               >  3:599985/1‑150 (MQ=255)

                                                                                                 gCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTAc                                              <  2:87649/150‑1 (MQ=255)

                                                                                                     gcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCata                                                                                                                      <  2:1148216/74‑1 (MQ=255)

                                                                                                     gcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCata                                                                                                                      >  1:1148216/1‑74 (MQ=255)

                                                                                                         ggCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAGAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCaa                                      <  4:640566/150‑1 (MQ=255)

                                                                                                                       cGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCt                                                                        <  2:231429/102‑1 (MQ=255)

                                                                                                                       cGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCt                                                                        >  1:231429/1‑102 (MQ=255)

                                                                                                                        ggACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCaaa                       >  1:191598/1‑150 (MQ=255)

                                                                                                                        ggACCGCAGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCaaa                       >  2:1371897/1‑150 (MQ=255)

                                                                                                                         gACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcaca                                                            >  1:743772/1‑112 (MQ=255)

                                                                                                                         gACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcaca                                                            <  2:743772/112‑1 (MQ=255)

                                                                                                                          aCCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACt                     <  1:269943/150‑1 (MQ=255)

                                                                                                                            cGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCg                   <  2:1104371/150‑1 (MQ=255)

                                                                                                                            cGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCGACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCg                   <  1:404162/150‑1 (MQ=255)

                                                                                                                             gCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGa                  <  2:526763/150‑1 (MQ=255)

                                                                                                                              cTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGAt                 <  4:497686/150‑1 (MQ=255)

                                                                                                                                gCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACTAGACCTTTCCAAAGCTGGAAATGTATCCGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTg               >  3:781179/1‑150 (MQ=255)

                                                                                                                                 cacaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCGAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGAtt                >  1:139359/1‑148 (MQ=255)

                                                                                                                                 cacaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCGAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGAtt                <  2:139359/148‑1 (MQ=255)

                                                                                                                                 cacaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCCGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGc              <  4:781179/150‑1 (MQ=255)

                                                                                                                                  acaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCg             <  2:191598/150‑1 (MQ=255)

                                                                                                                                    aTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGCTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTg           >  3:714525/1‑150 (MQ=255)

                                                                                                                                     ttGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccac                                                     >  4:818162/1‑107 (MQ=255)

                                                                                                                                     ttGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccac                                                     <  3:818162/107‑1 (MQ=255)

                                                                                                                                       ggTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGCTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATc        <  4:714525/150‑1 (MQ=255)

                                                                                                                                         tttGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCt      >  3:8341/1‑150 (MQ=255)

                                                                                                                                         tttGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAg                                                                >  1:913751/1‑92 (MQ=255)

                                                                                                                                         tttGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAg                                                                <  2:913751/92‑1 (MQ=255)

                                                                                                                                          ttGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTc     >  1:819541/1‑150 (MQ=255)

                                                                                                                                             aTTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTcgcg  >  1:396988/1‑150 (MQ=255)

ACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCG  >  NC_000913_3_pae_tpiA/4158556‑4158846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A