Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
67 |
36.0 |
1614110 |
95.1% |
1535018 |
114.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,248,780 |
C→T |
R280Q (CGG→CAG) |
yeiE ← |
putative DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,248,780 | 0 | C | T | 100.0%
| 129.0
/ NA
| 37 | R280Q (CGG→CAG) | yeiE | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/18); total (19/18) |
GTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGG > NC_000913/2248646‑2248911
|
gTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAg < 2:209123/139‑1 (MQ=255)
aaGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAgcgc > 1:533212/1‑139 (MQ=255)
gcagcCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGt > 1:719320/1‑139 (MQ=255)
gcCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTgg > 2:414609/1‑139 (MQ=255)
tGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc < 2:454186/139‑1 (MQ=255)
ggCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGcc < 2:45744/139‑1 (MQ=255)
aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg < 2:602362/139‑1 (MQ=255)
aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg < 1:726940/139‑1 (MQ=255)
aaGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTACGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGcc > 2:469878/1‑139 (MQ=255)
aGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCca < 2:199673/139‑1 (MQ=255)
aGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCca < 1:414609/139‑1 (MQ=255)
gcgcTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca > 1:385803/1‑139 (MQ=255)
gcgcTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGAGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca > 2:506782/1‑139 (MQ=255)
cTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc < 1:192520/117‑1 (MQ=255)
cTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc > 2:192520/1‑117 (MQ=255)
gCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTa < 2:385803/139‑1 (MQ=255)
cTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg > 1:90206/1‑85 (MQ=255)
cTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg < 2:90206/85‑1 (MQ=255)
ttGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCa < 1:359854/139‑1 (MQ=255)
ttGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCa > 2:623766/1‑139 (MQ=255)
cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAg < 1:194774/64‑1 (MQ=255)
cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAg > 2:194774/1‑64 (MQ=255)
cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAgg < 2:157222/118‑1 (MQ=255)
cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAgg > 1:157222/1‑118 (MQ=255)
ttAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgcct < 1:469878/139‑1 (MQ=255)
cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAt > 2:62460/1‑139 (MQ=255)
aTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGAGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGa < 1:506782/139‑1 (MQ=255)
ttGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATc < 1:62460/139‑1 (MQ=255)
cGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgcgc < 1:128242/89‑1 (MQ=255)
cGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgcgc > 2:128242/1‑89 (MQ=255)
cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAAc < 1:556192/90‑1 (MQ=255)
cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAAc > 2:556192/1‑90 (MQ=255)
cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGAc > 1:377935/1‑139 (MQ=255)
aaaGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAgg > 2:221152/1‑139 (MQ=255)
aaaGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAgg > 2:564571/1‑139 (MQ=255)
aaaGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAgg > 1:128463/1‑139 (MQ=255)
cgcTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg > 2:663047/1‑35 (MQ=255)
|
GTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGG > NC_000913/2248646‑2248911
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
