Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 63 64.8 2934156 93.7% 2749304 111.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,248,780 C→T R280Q (CGG→CAG)  yeiE ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,248,7800CT100.0% 206.0 / NA 58R280Q (CGG→CAG) yeiEputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/26);  total (32/26)

TAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGC  >  NC_000913/2248647‑2248912
                                                                                                                                     |                                                                                                                                     
tAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAgc                                                                                                                                  >  1:1187571/1‑139 (MQ=255)
tAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAg                                                                                                                                   >  1:908909/1‑138 (MQ=255)
tAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAg                                                                                                                                   <  2:908909/138‑1 (MQ=255)
 aaaGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAgcg                                                                                                                                 >  2:1165453/1‑139 (MQ=255)
  aaGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAgcgc                                                                                                                                >  1:1274107/1‑139 (MQ=255)
  aaGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAgcgc                                                                                                                                >  1:1381880/1‑139 (MQ=255)
   agcagcCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGcgcg                                                                                                                               >  1:231500/1‑139 (MQ=255)
      agcCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTg                                                                                                                            >  1:553852/1‑139 (MQ=255)
        ccAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGa                                                                                                                          >  2:1389566/1‑139 (MQ=255)
                ttttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGtt                                                                                                                  >  1:586509/1‑139 (MQ=255)
                  ttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGtttt                                                                                                                >  2:617954/1‑139 (MQ=255)
                  ttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTCTAGCGCGTTGGAAAGGTGtttt                                                                                                                >  2:83052/1‑139 (MQ=255)
                  ttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCACCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGtttt                                                                                                                <  2:263510/139‑1 (MQ=255)
                       gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                           <  2:20631/139‑1 (MQ=255)
                       gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTCTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                           <  1:83052/139‑1 (MQ=255)
                        cGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCga                                                                                                          <  2:731906/139‑1 (MQ=255)
                               atgcTGAAGGCGCTGCGTCACGAAAACGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGta                                                                                                   <  2:1177815/139‑1 (MQ=255)
                                      aGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCca                                                                                            >  1:1087092/1‑139 (MQ=255)
                                       ggCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcac                                                                                           >  1:1348525/1‑139 (MQ=255)
                                           cTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACg                                                                                       <  2:249778/139‑1 (MQ=255)
                                           cTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACg                                                                                       <  2:311648/139‑1 (MQ=255)
                                                  aCGAAATCGACACAGCACCAGCATG‑TCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATc                                                                               >  2:191903/1‑139 (MQ=255)
                                                       aTCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgc                                                                           <  1:629040/139‑1 (MQ=255)
                                                       aTCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgc                                                                           <  2:1087092/139‑1 (MQ=255)
                                                        tCGACACAGCACCAGCATG‑TCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgcgc                                                                         <  1:191903/139‑1 (MQ=255)
                                                         cGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgcgc                                                                         >  1:218067/1‑139 (MQ=255)
                                                                 cACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑gg                                                                >  2:403783/1‑139 (MQ=255)
                                                                    cAGCATGTTCTTGTACAGTAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGAc                                                             <  1:986715/139‑1 (MQ=255)
                                                                           ttCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCg                                                           >  1:589413/1‑134 (MQ=255)
                                                                           ttCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCg                                                           <  2:589413/134‑1 (MQ=255)
                                                                            tCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACtt                                                     <  2:553852/139‑1 (MQ=255)
                                                                                 tACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACtt                                                >  2:1114543/1‑139 (MQ=255)
                                                                                   cAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCAATTaa                                              >  2:20419/1‑139 (MQ=255)
                                                                                     gCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAAtg                                            >  1:61687/1‑139 (MQ=255)
                                                                                     gCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAAtg                                            >  1:1314378/1‑139 (MQ=255)
                                                                                      cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                        <  1:658225/79‑1 (MQ=255)
                                                                                      cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                        >  2:658225/1‑79 (MQ=255)
                                                                                      cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGAc                                                             <  2:1111096/121‑1 (MQ=255)
                                                                                      cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGAc                                                             >  1:1111096/1‑121 (MQ=255)
                                                                                      cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAAtgt                                           >  1:753049/1‑139 (MQ=255)
                                                                                      cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAAtgt                                           >  2:273546/1‑139 (MQ=255)
                                                                                      cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAAtgt                                           >  2:633826/1‑139 (MQ=255)
                                                                                          ttAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGtgcct                                       >  2:558520/1‑139 (MQ=255)
                                                                                            aaCGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGtgcctgc                                     <  2:1381880/139‑1 (MQ=255)
                                                                                                   cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAt                              >  1:1105007/1‑139 (MQ=255)
                                                                                                   cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAt                              <  2:267544/139‑1 (MQ=255)
                                                                                                    acaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAtt                             <  1:403783/139‑1 (MQ=255)
                                                                                                    acaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAtt                             <  1:273546/139‑1 (MQ=255)
                                                                                                     caTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAtt                             <  2:1187571/139‑1 (MQ=255)
                                                                                                                tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTaa                                              >  1:790514/1‑110 (MQ=255)
                                                                                                                tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTaa                                              <  2:790514/110‑1 (MQ=255)
                                                                                                                tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATca                 <  2:1274107/139‑1 (MQ=255)
                                                                                                                tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATca                 >  2:963106/1‑139 (MQ=255)
                                                                                                                         tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTcgcg        <  2:586509/139‑1 (MQ=255)
                                                                                                                           cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCa                                                   >  2:387635/1‑94 (MQ=255)
                                                                                                                           cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCa                                                   <  1:387635/94‑1 (MQ=255)
                                                                                                                           cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGAc      >  2:73389/1‑139 (MQ=255)
                                                                                                                              aaaGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATC‑TCAATCACACGTCGCGACAGGc  <  1:1389566/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGA‑GGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGC  >  NC_000913/2248647‑2248912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A