Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	77	35.1	1569012	96.6%	1515665	112.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,248,780	C→T	R280Q (CGG→CAG)	yeiE ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,248,780	0	C	T	100.0%	150.7 / NA	43	R280Q (CGG→CAG)	yeiE	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/21); total (22/21)

AGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGG  >  NC_000913/2248653‑2248911

agcCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTg                                                                                                                          >  2:516257/1‑139 (MQ=255)

  ccAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGa                                                                                                                        >  2:710165/1‑139 (MQ=255)

  ccAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGa                                                                                                                        >  1:566772/1‑139 (MQ=255)

  ccAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGa                                                                                                                        >  2:563418/1‑139 (MQ=255)

    aaTCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGaaa                                                                                                                      >  1:45302/1‑139 (MQ=255)

              cTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTg                                                                                                            <  2:45302/139‑1 (MQ=255)

               tGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                           <  1:516257/139‑1 (MQ=255)

                    aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      <  2:331553/139‑1 (MQ=255)

                    aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      >  1:271600/1‑139 (MQ=255)

                    aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      >  1:410700/1‑139 (MQ=255)

                    aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAAATTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      <  2:566772/139‑1 (MQ=255)

                          tgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGtat                                                                                                <  1:103310/139‑1 (MQ=255)

                          tgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGtat                                                                                                <  1:710165/139‑1 (MQ=255)

                             tGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACg                                                                                             <  1:492674/139‑1 (MQ=255)

                                                  tCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACg                                                                                     <  1:182015/126‑1 (MQ=255)

                                                  tCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACg                                                                                     >  2:182015/1‑126 (MQ=255)

                                                                        ttGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAgg                                                               >  1:145527/1‑126 (MQ=255)

                                                                        ttGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAgg                                                               <  2:145527/126‑1 (MQ=255)

                                                                          gTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCg                                                          >  1:511684/1‑129 (MQ=255)

                                                                          gTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCg                                                          <  2:511684/129‑1 (MQ=255)

                                                                                cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                         >  2:92604/1‑76 (MQ=255)

                                                                                cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                         <  1:92604/76‑1 (MQ=255)

                                                                                cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTa                                                                                   >  2:603687/1‑98 (MQ=255)

                                                                                cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTa                                                                                   <  1:603687/98‑1 (MQ=255)

                                                                                cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAAtgt                                          >  1:702017/1‑139 (MQ=255)

                                                                                 aaCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGcc                                                                                           >  2:3496/1‑89 (MQ=255)

                                                                                 aaCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGcc                                                                                           <  1:3496/89‑1 (MQ=255)

                                                                                  aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca                                                                                        >  2:173367/1‑91 (MQ=255)

                                                                                  aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca                                                                                        <  1:173367/91‑1 (MQ=255)

                                                                                    ttAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca                                                                                        >  2:274740/1‑89 (MQ=255)

                                                                                    ttAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca                                                                                        <  1:274740/89‑1 (MQ=255)

                                                                                             cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAt                             <  2:150038/139‑1 (MQ=255)

                                                                                             cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAt                             >  2:528714/1‑139 (MQ=255)

                                                                                               caTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTg                           <  1:118086/139‑1 (MQ=255)

                                                                                                  ttGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATc                        <  1:323517/139‑1 (MQ=255)

                                                                                                           cGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCACAACTTCACTTAATGtgcctgcc                                   >  2:211722/1‑119 (MQ=255)

                                                                                                           cGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCACAACTTCACTTAATGtgcctgcc                                   <  1:211722/119‑1 (MQ=255)

                                                                                                                   tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTa                                                                                   <  2:146448/63‑1 (MQ=255)

                                                                                                                   tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTa                                                                                   >  1:146448/1‑63 (MQ=255)

                                                                                                                   tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTa                                              <  2:638813/100‑1 (MQ=255)

                                                                                                                   tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTa                                              >  1:638813/1‑100 (MQ=255)

                                                                                                                   tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTcgcg       >  2:400274/1‑139 (MQ=255)

                                                                                                                        aaaGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAgg  >  2:373936/1‑139 (MQ=255)

AGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGG  >  NC_000913/2248653‑2248911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A