Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	78	65.3	3016648	94.6%	2853749	111.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,292	G→A	A315T (GCG→ACG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,292	0	G	A	100.0%	239.9 / NA	68	A315T (GCG→ACG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/36); total (32/36)

CGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAG  >  NC_000913/4186157‑4186421

cgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGa                                                                                                                                >  1:225419/1‑139 (MQ=255)

  tcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATc                                                                                                                              >  1:1002675/1‑139 (MQ=255)

   cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCa                                                                                                                             >  2:1072762/1‑139 (MQ=255)

   cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCa                                                                                                                             >  1:246644/1‑139 (MQ=255)

    gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            <  1:1158430/139‑1 (MQ=255)

    gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            <  1:824368/139‑1 (MQ=255)

                  aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATTATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            >  2:1064402/1‑125 (MQ=255)

                  aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            <  1:1064402/125‑1 (MQ=255)

                  aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGc                                                                                                              >  1:549578/1‑139 (MQ=255)

                  aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGc                                                                                                              >  2:1475233/1‑139 (MQ=255)

                   acaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCg                                                                                                             >  1:779730/1‑139 (MQ=255)

                                    ctgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTgg                                                                                            >  1:1283825/1‑139 (MQ=255)

                                     tgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                                                           >  2:273070/1‑139 (MQ=255)

                                      gctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                                                          >  1:478317/1‑139 (MQ=255)

                                      gctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                                                          <  1:403053/139‑1 (MQ=255)

                                         ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc                                                                                       <  2:1283825/139‑1 (MQ=255)

                                          gATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACa                                                                                      <  1:1475233/139‑1 (MQ=255)

                                          gATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACa                                                                                      <  2:779730/139‑1 (MQ=255)

                                              tGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  <  1:755841/139‑1 (MQ=255)

                                              tGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  <  2:1042408/139‑1 (MQ=255)

                                               ggCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATc                                                                                 <  1:1034368/139‑1 (MQ=255)

                                                gCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCa                                                                                >  1:1149993/1‑139 (MQ=255)

                                                 cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                                                           >  1:1313263/1‑127 (MQ=255)

                                                 cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                                                           <  2:1313263/127‑1 (MQ=255)

                                                    cgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc                                                                                       >  2:454183/1‑128 (MQ=255)

                                                    cgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc                                                                                       <  1:454183/128‑1 (MQ=255)

                                                      ccGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAAc                                                                                                                  <  1:519097/99‑1 (MQ=255)

                                                      ccGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAAc                                                                                                                  >  2:519097/1‑99 (MQ=255)

                                                             tcatcGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAggg                                                                   <  2:549578/139‑1 (MQ=255)

                                                              catcGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGt                                                                  >  1:1387012/1‑139 (MQ=255)

                                                                   tacgtaACGAAAAACGTATGCTGGAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGttt                                                             <  2:8609/139‑1 (MQ=255)

                                                                        aaCGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTc                                                        >  2:1103741/1‑139 (MQ=255)

                                                                          cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  <  1:663282/111‑1 (MQ=255)

                                                                          cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  >  2:663282/1‑111 (MQ=255)

                                                                          cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                                                      >  2:1242539/1‑139 (MQ=255)

                                                                           gAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGa                                                     <  2:173986/139‑1 (MQ=255)

                                                                              aaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAAcc                                                  <  1:1103741/139‑1 (MQ=255)

                                                                                    aTGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTTGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCg                                            >  1:1391509/1‑139 (MQ=255)

                                                                                       ctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa                                         <  1:57094/139‑1 (MQ=255)

                                                                                          cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGAt                                                                                                                               <  2:127219/50‑1 (MQ=255)

                                                                                          cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGAt                                                                                                                               >  1:127219/1‑50 (MQ=255)

                                                                                          cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc                                      <  2:1149993/139‑1 (MQ=255)

                                                                                            ggAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgt                                    <  2:917731/139‑1 (MQ=255)

                                                                                               aGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGtt                                 <  1:273070/139‑1 (MQ=255)

                                                                                                  ggTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            >  1:630197/1‑45 (MQ=255)

                                                                                                  ggTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            <  2:630197/45‑1 (MQ=255)

                                                                                                    tAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCt                                              >  2:1276508/1‑121 (MQ=255)

                                                                                                    tAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCt                                              <  1:1276508/121‑1 (MQ=255)

                                                                                                           ccTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGt                     >  2:855271/1‑139 (MQ=255)

                                                                                                           ccTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGt                     >  2:1384882/1‑139 (MQ=255)

                                                                                                           ccTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGt                     >  1:375742/1‑139 (MQ=255)

                                                                                                            ctgctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTc                    <  2:375742/139‑1 (MQ=255)

                                                                                                              gctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGt                  >  1:364762/1‑139 (MQ=255)

                                                                                                               ctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGtt                 <  1:1242539/139‑1 (MQ=255)

                                                                                                                      aCGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAAt          <  1:791747/139‑1 (MQ=255)

                                                                                                                      aCGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAAt          <  2:1387012/139‑1 (MQ=255)

                                                                                                                      aCGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAAt          <  2:225419/139‑1 (MQ=255)

                                                                                                                         gtcgtcGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCAc       >  1:1317700/1‑139 (MQ=255)

                                                                                                                            gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGt                                                                           <  1:1168426/68‑1 (MQ=255)

                                                                                                                            gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGt                                                                           >  2:1168426/1‑68 (MQ=255)

                                                                                                                            gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    <  1:1072762/139‑1 (MQ=255)

                                                                                                                            gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    <  1:1296419/139‑1 (MQ=255)

                                                                                                                            gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    >  2:287400/1‑139 (MQ=255)

                                                                                                                            gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    <  2:246644/139‑1 (MQ=255)

                                                                                                                            gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    <  1:940572/139‑1 (MQ=255)

                                                                                                                            gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    >  1:966798/1‑139 (MQ=255)

                                                                                                                              cgcgGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAg  <  1:61955/139‑1 (MQ=255)

                                                                                                                                    cGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  >  2:1137486/1‑53 (MQ=255)

CGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAG  >  NC_000913/4186157‑4186421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A