Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	81	28.9	1316934	95.8%	1261622	113.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,292	G→A	A315T (GCG→ACG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,292	0	G	A	100.0%	135.6 / NA	40	A315T (GCG→ACG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/20); total (20/20)

GCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGT  >  NC_000913/4186161‑4186423

gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              >  2:38568/1‑139 (MQ=255)

gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              <  2:210389/139‑1 (MQ=255)

gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              >  1:418706/1‑139 (MQ=255)

 cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGGCGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAcc                                                                                                                             >  1:414568/1‑139 (MQ=255)

 cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAcc                                                                                                                             >  1:82908/1‑139 (MQ=255)

 cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              >  2:95307/1‑138 (MQ=255)

 cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              <  1:95307/138‑1 (MQ=255)

       tAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCt                                                                                                                       <  2:414568/139‑1 (MQ=255)

                caacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGt                                                                                                              <  2:161521/139‑1 (MQ=255)

                     gTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCctct                                                                                                         >  1:103177/1‑139 (MQ=255)

                                     ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAGTCTTTGGCCGAc                                                                                         <  2:28799/139‑1 (MQ=255)

                                        tCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATg                                                                                      >  1:610260/1‑139 (MQ=255)

                                          tGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    <  2:103177/139‑1 (MQ=255)

                                            gCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCa                                                                                  >  1:43599/1‑139 (MQ=255)

                                             cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaa                                                                                 >  1:561197/1‑139 (MQ=255)

                                             cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaa                                                                                 >  2:181534/1‑139 (MQ=255)

                                                                 cgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTcc                                                             >  1:588603/1‑139 (MQ=255)

                                                                      cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                                                        >  2:498578/1‑139 (MQ=255)

                                                                       gAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGa                                                       <  1:260832/139‑1 (MQ=255)

                                                                        aaaaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGaa                                                      <  2:610260/139‑1 (MQ=255)

                                                                                   ctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa                                           >  2:72587/1‑139 (MQ=255)

                                                                                      cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc                                        >  1:563859/1‑139 (MQ=255)

                                                                                                   aCGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTAAAATCTTTCGCCGACATGATCAAAGGTAAACAGGGTCGTTTACGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTc                           >  1:166957/1‑139 (MQ=255)

                                                                                                      cccTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCgg                        <  2:418706/139‑1 (MQ=255)

                                                                                                        ctgctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTc                      <  2:563859/139‑1 (MQ=255)

                                                                                                          gctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGt                    >  2:107840/1‑139 (MQ=255)

                                                                                                          gctgGATAACGGTCGTCGCGGCCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGt                    <  1:200713/139‑1 (MQ=255)

                                                                                                            tgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTc                  <  2:43599/139‑1 (MQ=255)

                                                                                                                tAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTa              >  1:25121/1‑139 (MQ=255)

                                                                                                                     gtcgtcGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTg                                                  <  2:560034/98‑1 (MQ=255)

                                                                                                                     gtcgtcGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTg                                                  >  1:560034/1‑98 (MQ=255)

                                                                                                                       cgtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCg       <  1:181534/139‑1 (MQ=255)

                                                                                                                        gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    >  2:500520/1‑61 (MQ=255)

                                                                                                                        gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    <  1:500520/61‑1 (MQ=255)

                                                                                                                        gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCgg                        <  1:89822/121‑1 (MQ=255)

                                                                                                                        gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCgg                        >  2:89822/1‑121 (MQ=255)

                                                                                                                        gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt      <  2:561197/139‑1 (MQ=255)

                                                                                                                        gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt      <  2:25121/139‑1 (MQ=255)

                                                                                                                        gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt      <  2:588603/139‑1 (MQ=255)

                                                                                                                            cgGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGt  <  2:166957/139‑1 (MQ=255)

GCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGT  >  NC_000913/4186161‑4186423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A