Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 177 96.4 2178490 87.4% 1904000 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,828,506 T→G 25.0% V257G (GTG→GGG)  srlD → sorbitol‑6‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,828,5060TG25.0% 113.9 / 5.4 54V257G (GTG→GGG) srlDsorbitol‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (3/35);  new base G (14/2);  total (17/37)
Fisher's exact test for biased strand distribution p-value = 2.17e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGG  >  NC_000913/2828269‑2828738
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
cGGTGCATTGCTTGATGCCCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGGTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg                                                                                                                                                                                                                                              <  1:1088462/239‑1 (MQ=255)
cGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg                                                                                                                                                                                                                                              <  1:302746/239‑1 (MQ=255)
 caggCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg                                                                                                                                                                                                                                              <  1:795764/235‑1 (MQ=255)
   tGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑Tg                                                                                                                                                                                                                                          >  1:697396/1‑239 (MQ=255)
   gtccttCCCTGGTGCCCGGTAACCTTCTTAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑Tg                                                                                                                                                                                                                                          <  2:341942/235‑1 (MQ=255)
        tcctcggtccTCGTTAACCTGCTGAATTCGCCGATGTTCCCGTCTCTGTTGCCAAAATACGCGACCAAGCGGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCATTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTg                                                                                                                                                                                                                                     <  2:510913/230‑1 (MQ=255)
        tccctggttgccGGTAACCGTGTGAAATCGCCGATTTTCCAGTCACTGTTCCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTg                                                                                                                                                                                                                                     <  2:104982/228‑1 (MQ=255)
               tGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGCCAGTCGGTCAATGTCCCCGGCGGGCCGGGGG‑TGTTcccggtcttt                                                                                                                                                                                                                              >  2:495493/1‑230 (MQ=255)
               tGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑AGTTTTgtttcaaa                                                                                                                                                                                                                              >  1:183503/1‑232 (MQ=255)
                gCTCGGTAACCCGCTGAAATCGCCGAGTTTCCATGCACCGTTGCCACAATACGCGACCAAGCGTGGGATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGGCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAg                                                                                                                                                                                                                             <  2:860409/239‑1 (MQ=255)
                     gttcccTGCTTAATTCCCCGTTTTTCCCGTCCCTTTTGCCAAAATACGCGCACAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGgag                                                                                                                                                                                                                        <  2:960795/235‑1 (MQ=255)
                       aaCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGAACAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGgaga                                                                                                                                                                                                                       <  1:760324/238‑1 (MQ=255)
                        aCCTGCTGAAATCGCCTGTGTTCCAGTCACTGTTGCCACAATCCGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATc                                                                                                                                                                                                                     <  1:158911/239‑1 (MQ=255)
                              tgAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGG‑TGTTCTGATCACACGCGGAGATACCTTaa                                                                                                                                                                                                               >  1:974916/1‑239 (MQ=255)
                                  aTCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGTTTAACAGCGGGGATCCATTAAggg                                                                                                                                                                                                            >  1:624529/1‑237 (MQ=255)
                                  aTCGCCGATGTTCCAGTCACTGTGACCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGAt                                                                                                                                                                                                           <  1:658352/239‑1 (MQ=255)
                                       cGATGTTCCAGTCACTTGTGACACAATACGCGACCAAGCTGAGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGGCAAAGTACCGCTCAAACGCGGCTGCGATTAACAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                      <  1:1048275/239‑1 (MQ=255)
                                       cGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                      <  1:8225/239‑1 (MQ=255)
                                         aTGTTACAGTCCCTGTTGCCCCAATACGGGACCAAGCTGGGTAGCAAACCGGAGCAAGTCCATCAGGATTACTACGACAAAGTCCCGCTCAACAGGGGCTGCGATTAGCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTg                                                                                                                                                                                                    <  2:355651/239‑1 (MQ=255)
                                          ttttccctttaCTTTTCCCACAATTCGCCACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                                                                                   <  2:715235/229‑1 (MQ=255)
                                           gTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTCCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                                                                                   <  1:591252/238‑1 (MQ=255)
                                           gTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                                                                                   <  1:768403/238‑1 (MQ=255)
                                             tcccggtccTGTTGCCCCAATACGGGACCAAGCTGGGTTCCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTACCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGAc                                                                                                                                                                                                <  2:753790/231‑1 (MQ=255)
                                                  tCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATTAACAGCGGGGGTCCCTTAAGGATTTCCCTGAGACtataa                                                                                                                                                                                           >  1:694925/1‑238 (MQ=255)
                                                    aCTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGG‑TGTTTTTGTTACCAGCGGAGATCCATTAATGATCTCCGTGCGACtcacaaa                                                                                                                                                                                         >  1:1031134/1‑233 (MQ=255)
                                                      tGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTg                                                                                                                                                                                                                                     <  1:665977/193‑1 (MQ=255)
                                                      tGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGCCAAGGGAGAGTTTTGTTCAACCACGGCATCCCATAAAGGATCCCCGCgagatataaaaa                                                                                                                                                                                         >  2:473243/1‑230 (MQ=255)
                                                       gTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTTTGGTCAACCACGGCGAACCCTTAAGGATATCCCTgggagcatacgata                                                                                                                                                                                       >  1:753790/1‑228 (MQ=255)
                                                                    ccgACCAAGGTTGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACg                                                                                                                                                                         <  2:183503/238‑1 (MQ=255)
                                                                         cAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcat                                                                                                                                                                    <  1:379413/239‑1 (MQ=255)
                                                                              tGGGTTTAAACCCGGATTAAGTCGAGCCGTATTAACTCGACAAAGTCCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg                                                                                                                                                                 <  2:1031900/237‑1 (MQ=255)
                                                                              tGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg                                                                                                                                                                 <  1:252183/237‑1 (MQ=255)
                                                                               tggTATTAAACCGGATCAAGTCGAGCAGTATTGCAGCGAAAAAGTCCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAgg                                                                                                                                                                <  2:974916/236‑1 (MQ=255)
                                                                                            gATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGGTCTGATCAAAAGCGGAGATCCATTAAGGATCTCCGGGGGACTATAGAATGCCTGATGCGCCACCCCCATCCGGCCTACAGGGCTTcc                                                                                                                                                  >  1:342189/1‑238 (MQ=255)
                                                                                                  gTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGA‑TGTTTTTGTCAACAGAGGAGATACATTAAGGATCTCCGCGAGACCATAAAATACCCGATGCGCCACGCCCATCAGGCCAACAGGACCTCccccccac                                                                                                                                           >  2:687640/1‑235 (MQ=255)
                                                                                                       gCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGG‑TGTTCTGATCAACAGCGGAGAGCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTACCTTa                                                                                                                                      >  1:305637/1‑239 (MQ=255)
                                                                                                          gTATTACATCGACAAAGGCCCGCACAAACGGGGGTGCGATTAGAAAGGTGGGCGGAATAGGGTGGTGTTCTACCCCAGTCCAAAGGGGTCGTACTGCCCGGGAAAGTCGATCAATGTCCCGGGCGGTCAGGTGA‑TGTTCTGAAAAACAGCGGAGATCCATTAAGGATCTCCGGGAGCCTATAGAATGCCTGATGCGCTACGCTCATCGGGCATACAGGACTTCCGCCACTACATTAAgg                                                                                                                                   <  2:342189/239‑1 (MQ=255)
                                                                                                           tATTACATCGACAAAGTCCCGCTAAAACGGGGCTGCGATTAACAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCCCCGGACAGTCGATCAATGTCACCGGGGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCa                                                                                                                                              <  1:249435/227‑1 (MQ=255)
                                                                                                                             ccGCTCAACCGCGGCTGCTATTATCAAGATGTGCTGAATCTGCTGCTGTTCTACGCCAGTCTAAAGGCGTCGTCCTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATAAACAGGGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCTTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCa                                                                                                                <  1:304565/239‑1 (MQ=255)
                                                                                                                                            cgcatttATCAAGGTGTGCTGAATTGGCTGCTGTTCAACGCCAGTCCTAAGGCGTCGTACGGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATAAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGc                                                                                                  <  1:687640/233‑1 (MQ=255)
                                                                                                                                                gtttcaagtaTGGGCTGATTGTGCTGTTGTTCTCCGCCAGCCCAAAGGCGTCCTACGGCCGCGGACGGTCCAACATGCGCACCGGCGGCAGGGTGA‑TGTTCTGATCACCAGGGGAGACCCTTAAAGGAGCTCGGGGAGACTAAAGAATGCCTGTGGCGCTCCGCTCATCCGGCATACAGGCTTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGtt                                                                                              <  2:697396/229‑1 (MQ=255)
                                                                                                                                                aTTATCAAGATGTGCTGAATATGCTGCTGTTCTCCGCCAGCCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCCCTCCGCTCATCAGGCATCCAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGtt                                                                                              <  1:604932/238‑1 (MQ=255)
                                                                                                                                                    tCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTg                                                                                                                                                                                                    <  1:803263/132‑1 (MQ=255)
                                                                                                                                                                 aaTTTGCTGCTGTTCTACCCCAGCCCTAAGGCGTCGTACGGCACCGGACAGTCGATCAATGCCCCCGGCGGTCAGGTAA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCaa                                                                           <  1:329255/239‑1 (MQ=255)
                                                                                                                                                                  attttgtgttgTTCTACGCCAGTCTGAGGGGGTCGGCCTGCGCCGGACAGTCGATAAATGTCACCGGGGGTCAGGTGA‑TGTTCGGATCAACAGGGGAGATCCATTAAGGATCTCCGGGAGACTATAGAATGCCTGATGCGCTACGTGCATCGGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAAc                                                                          <  2:412961/230‑1 (MQ=255)
                                                                                                                                                                                             aGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAACAGCGGGGATCCATTAAGGATCTCCGTGAGACTAAAGAATGCCTGAAGCGCCACGCCCATCCAGCCTAACAGACTTCCCCCACCACCTTAATGAAAAGATATGGGATCCCCACTCATCACCCCCCCCGCTATCGCCCGGTGGG‑GGCAACCGGCCCTAAGGCGCG‑GGGCA‑AAtt                                                >  2:365922/1‑236 (MQ=255)
                                                                                                                                                                                                          cACCGGACAGTCGATAAATGTCACGGGCGGTAGGGTGA‑TGTGCTGATAACCGGCGGAGATCAATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGc                               <  1:365922/239‑1 (MQ=255)
                                                                                                                                                                                                           acgggACAGTGGATCAATGCCCCGGGCGGTCAGGGGA‑TGTTCTGACCAACGGCGGAGATCCATTAAGGATCGCGGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCGGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGc                               <  1:1002788/235‑1 (MQ=255)
                                                                                                                                                                                                               gACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCCTACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGCCGCCGTTATCGCCCTGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTTTCGTTTTAACCGTGCCTTc                           >  1:331446/1‑238 (MQ=255)
                                                                                                                                                                                                               gACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAACAGGGGGGATCCATTAAGGATCTCCGTGGGACTATAGAATACCTGATTCGCCACGCCCATCAGGCATACAGGACTTCCGCCACTACACTAAGGAAAAGTTATGGTATCCCCACTCATCACCGCCCCCGCTATCTCCCGGTTGT‑CGCCACTGG‑CCCTTGGCGGC‑CGGGA‑AAATTTTCGTTTTAACCATgtcctcc                          >  2:1064919/1‑233 (MQ=255)
                                                                                                                                                                                                                     cgacaaaTGGCCCCGGGGGTCGGGTGA‑TGTTCTGATCAAAGGGGGAGATCCATTAAGGCTCTCCGTGAGCCTATAGAATGCCTGATGCGCTACGTTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGCCTTCGacaca                     <  1:871601/233‑1 (MQ=255)
                                                                                                                                                                                                                       aTCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGGTCAACAGCGGAGATCCATTAAGGATCTCCGTGGGACTATAAAATGCCCGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTACATTAAGGAAAAGGTATGGGATCCGCACTCATCACCCTCGCCGTTATCTCCTGGTGTG‑GGAGACCAG‑CCTCAGGGGGC‑GGGCACAATTTTTCTTTTAAACCG‑GCCTTCGccacccaa                  >  2:940853/1‑235 (MQ=255)
                                                                                                                                                                                                                         cAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAACAGCGGAGATCCATTAAAGGGCTCCCGGAGACCATAGAATGCCTGATGCGCTACCCCCATCAGGCATACAGGACTTCCCCCACTCCATTAAGGGAAAGTTATGGTATCCGCACTCATCAACGGCCCCGTTATTGCCTGG‑GTGTTGCAACTGG‑CCCTAGGCGGCGTGGCA‑AATTT‑TCTTTTTTACCCGGCCCTCCccacaccca                 >  2:871601/1‑235 (MQ=255)
                                                                                                                                                                                                                                        tCAGGGGA‑TGTTCGGACCAACGGCGGGGATCCATTAAGGATCCCCGGGAGACTAAAGAAGGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCggg  <  1:940853/238‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
CGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGG  >  NC_000913/2828269‑2828738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A