Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F73 I0 R1 132 75.9 2634066 97.4% 2565580 143.6

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC  >  NC_000913/2173230‑2173505
                                                                                                                                     |                                                                                                                                              
ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGC                                                                                                                             >  NB501016:230:HFVJLAFXY:3:11606:10236:1281/1‑151 (MQ=60)
   cgtggcgatggcggTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAC                                                                                                                          >  NB501016:230:HFVJLAFXY:1:11103:6441:9178/15‑151 (MQ=60)
             GCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCA                                                                                                                 >  NB501016:230:HFVJLAFXY:2:21204:5063:6237/1‑150 (MQ=60)
               CATCGCCACGAACAAGCCGAAGGTGCCAACATCTCCAAACGGCAGCACCTGAATACCCGGCACACAAACAGCAATTAAAATGGTGAGTGCGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGG                                                                                                              <  NB501016:230:HFVJLAFXY:3:21507:16592:2661/151‑1 (MQ=60)
                  CGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACGTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTTGGATAAAAATCTGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCAGGATCAAGGCC                                                                                                            <  NB501016:230:HFVJLAFXY:2:21207:13777:8738/150‑1 (MQ=60)
                    CCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAT                                                                                                         <  NB501016:230:HFVJLAFXY:2:11303:15310:18996/151‑1 (MQ=60)
                      ACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCA                                                                                                       <  NB501016:230:HFVJLAFXY:2:11105:18988:13861/151‑1 (MQ=60)
                                            GATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTT                                                                                 >  NB501016:230:HFVJLAFXY:4:11403:4135:11237/1‑151 (MQ=60)
                                              TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC                                                                                >  NB501016:230:HFVJLAFXY:2:21202:21630:7329/1‑150 (MQ=60)
                                                GCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCC                                                                             <  NB501016:230:HFVJLAFXY:3:11611:17847:18118/151‑1 (MQ=60)
                                                  CAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTG                                                                           <  NB501016:230:HFVJLAFXY:1:11108:4454:3057/151‑1 (MQ=60)
                                                            CACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTA                                                                 <  NB501016:230:HFVJLAFXY:1:11306:17433:14240/151‑1 (MQ=60)
                                                            CACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCC                                                                                                            >  NB501016:230:HFVJLAFXY:3:21507:4348:1771/1‑108 (MQ=60)
                                                                TGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCC                                                                                                            >  NB501016:230:HFVJLAFXY:4:11607:10998:15650/1‑104 (MQ=60)
                                                                TGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCACAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACG                                                               >  NB501016:230:HFVJLAFXY:2:21108:13939:14958/1‑149 (MQ=60)
                                                                 GATTACCCGGCACACAACCCGCAATTAAAATGGTGAGTGGGATAAAAATCCGGCGTGCCGATACCA‑‑CCGCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCC                                                                             <  NB501016:230:HFVJLAFXY:4:21610:1072:2013/134‑1 (MQ=60)
                                                                 GATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGC                                                            >  NB501016:230:HFVJLAFXY:2:11108:16705:2564/1‑151 (MQ=60)
                                                                  ATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCC                                                           >  NB501016:230:HFVJLAFXY:3:21408:4816:18239/1‑151 (MQ=60)
                                                                             CACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAT                                                >  NB501016:230:HFVJLAFXY:1:11301:4499:6356/1‑151 (MQ=60)
                                                                               CAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGG                                              >  NB501016:230:HFVJLAFXY:2:11103:16571:10093/1‑151 (MQ=60)
                                                                                  ACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCG                                           <  NB501016:230:HFVJLAFXY:2:11307:19261:16254/151‑1 (MQ=60)
                                                                                   CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGT                                          <  NB501016:230:HFVJLAFXY:1:11112:6960:2630/151‑1 (MQ=60)
                                                                                      CAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTA                                        >  NB501016:230:HFVJLAFXY:3:11403:25328:13902/1‑150 (MQ=60)
                                                                                              ATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCA                               <  NB501016:230:HFVJLAFXY:1:21305:25207:11753/151‑1 (MQ=60)
                                                                                                        GGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTGT                     >  NB501016:230:HFVJLAFXY:4:21603:18368:10295/1‑151 (MQ=60)
                                                                                                         GATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTT                     >  NB501016:230:HFVJLAFXY:2:11304:25675:16460/1‑150 (MQ=60)
                                                                                                                ATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTACACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCC             >  NB501016:230:HFVJLAFXY:4:21405:23586:17363/1‑151 (MQ=60)
                                                                                                                  CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACTCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGT           <  NB501016:230:HFVJLAFXY:1:21202:16141:3156/151‑1 (MQ=60)
                                                                                                                     GCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGC        >  NB501016:230:HFVJLAFXY:2:11108:13249:14089/1‑151 (MQ=60)
                                                                                                                          CCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATT     >  NB501016:230:HFVJLAFXY:4:21509:19465:13058/1‑149 (MQ=60)
                                                                                                                           CGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC  >  NB501016:230:HFVJLAFXY:4:11505:8961:12673/1‑151 (MQ=60)
                                                                                                                           CGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC  <  NB501016:230:HFVJLAFXY:4:21612:2937:14154/151‑1 (MQ=60)
                                                                                                                            GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC  >  NB501016:230:HFVJLAFXY:4:11404:3811:18350/1‑150 (MQ=60)
                                                                                                                                     |                                                                                                                                              
ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC  >  NC_000913/2173230‑2173505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑