Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 80,603 (T)5→6 coding (262/1401 nt) leuC ← 3‑isopropylmalate isomerase (dehydratase) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091380,5981.T99.4% 1122.2 / ‑2.2 315coding (267/1401 nt)leuC3‑isopropylmalate isomerase (dehydratase) subunit
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (165/147);  total (166/149)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

GCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑TTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATG  >  NC_000913/80507‑80685
                                                                                            |                                                                                       
 cAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tt                                                                                       <  2:7765456/93‑1 (MQ=255)
 cAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tt                                                                                       <  1:5291177/93‑1 (MQ=255)
 cAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tt                                                                                       <  1:632892/93‑1 (MQ=255)
  aaGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑ttt                                                                                      <  1:1198846/93‑1 (MQ=255)
   aGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tttt                                                                                     >  2:7614436/1‑93 (MQ=255)
   aGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tttt                                                                                     <  1:2195111/93‑1 (MQ=255)
     gTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tttttt                                                                                   <  1:5546695/93‑2 (MQ=255)
     gTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tttttt                                                                                   <  1:2687060/93‑2 (MQ=255)
      tGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑ttttttg                                                                                  <  2:1651826/93‑3 (MQ=255)
      ggACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑ttttttg                                                                                  <  1:353784/92‑3 (MQ=255)
        aCGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑ttttttgat                                                                                <  2:6240696/93‑5 (MQ=255)
          gCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCa                                                                               <  2:7810583/93‑1 (MQ=255)
          gCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCa                                                                               <  1:3614843/93‑1 (MQ=255)
          ccccTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCa                                                                               <  2:1066637/92‑1 (MQ=255)
           cccTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAg                                                                              >  1:4735159/1‑93 (MQ=255)
            ccTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGt                                                                             <  1:3178479/93‑1 (MQ=255)
            ccTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGt                                                                             >  2:3843264/1‑93 (MQ=255)
             cTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGtt                                                                            >  1:2938927/1‑93 (MQ=255)
             cTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGtt                                                                            <  2:5181288/93‑1 (MQ=255)
             cTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGtt                                                                            >  2:7653787/1‑93 (MQ=255)
              tGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAACTTCTTTGCAGTTTTTTGATCAGTTc                                                                           >  2:1769847/1‑93 (MQ=255)
              tGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTc                                                                           >  1:7502216/1‑93 (MQ=255)
              tGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTc                                                                           >  1:6494243/1‑93 (MQ=255)
                ttCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCt                                                                         >  2:6935518/1‑93 (MQ=255)
                  ccGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGc                                                                       <  2:2917273/93‑1 (MQ=255)
                   cGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCa                                                                      >  2:6365607/1‑93 (MQ=255)
                    ggCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCAt                                                                     >  1:2763887/1‑93 (MQ=255)
                      ccccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCt                                                                   <  1:220615/93‑1 (MQ=255)
                      ccccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCCTTGCAGTTTTTTGATCATTTCCTGCATCt                                                                   >  1:801383/1‑93 (MQ=255)
                       cccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTg                                                                  >  2:7332242/1‑93 (MQ=255)
                       cccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGATTTTTGATCAGTTCCTGCATCTg                                                                  >  2:5654654/1‑93 (MQ=255)
                        ccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTgg                                                                 >  1:186276/1‑93 (MQ=255)
                         cATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGa                                                                >  2:5592094/1‑93 (MQ=255)
                         cATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGa                                                                >  2:495831/1‑93 (MQ=255)
                          aTTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGAt                                                               <  2:2020284/93‑1 (MQ=255)
                          aTTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGAt                                                               >  1:3060625/1‑93 (MQ=255)
                             aCGTGGACGATCCCCTGATACGGCTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATAcg                                                            <  1:246899/93‑1 (MQ=255)
                              cGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATAcgc                                                           >  1:1685714/1‑93 (MQ=255)
                                  gACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCa                                                       <  2:1264951/93‑1 (MQ=255)
                                   gcGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCAt                                                      <  1:1026455/92‑1 (MQ=255)
                                   aCGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCAt                                                      <  2:2073740/93‑1 (MQ=255)
                                      aTCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTc                                                   >  1:4036877/1‑93 (MQ=255)
                                      aTCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTc                                                   >  1:6927710/1‑93 (MQ=255)
                                      aTCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTc                                                   >  1:5029078/1‑93 (MQ=255)
                                       tCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCa                                                  >  2:7242417/1‑93 (MQ=255)
                                          ccTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCg                                               <  2:5503532/93‑1 (MQ=255)
                                          ccTGATACGGGCGATTCAGGTCATACAGTTCGACGCCAACTTCTTTGCAGTTTTTTGATCAGCTCCTGCATCTGGATACGCCCCATTTCACCg                                               >  2:656092/1‑93 (MQ=255)
                                           cTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGc                                              >  2:2778660/1‑93 (MQ=255)
                                           cTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGc                                              >  2:2472695/1‑93 (MQ=255)
                                           cTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGc                                              <  1:7091919/93‑1 (MQ=255)
                                           cTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGc                                              <  1:2305701/93‑1 (MQ=255)
                                           cTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGc                                              <  1:4057679/93‑1 (MQ=255)
                                             gATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAg                                            <  1:5787742/93‑1 (MQ=255)
                                              aTACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAgg                                           >  2:1191759/1‑93 (MQ=255)
                                              aTACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAgg                                           >  1:160835/1‑93 (MQ=255)
                                                aCGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCa                                         <  2:5766615/93‑1 (MQ=255)
                                                aCGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCa                                         <  2:2543715/93‑1 (MQ=255)
                                                aCGGGTGATTCAGGTCACACAGTTCGACGCCAAATTCTTTGCAG‑TTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCAt                                        <  1:2059474/93‑1 (MQ=255)
                                                 cGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCAt                                        <  1:4344456/93‑1 (MQ=255)
                                                  gggTGATTCAGGTCATACCGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCAtt                                       >  1:1791030/1‑93 (MQ=255)
                                                   ggTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTa                                      <  2:96011/93‑1 (MQ=255)
                                                    gTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTaa                                     <  2:3459971/93‑1 (MQ=255)
                                                    gTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTaa                                     <  1:7297852/93‑1 (MQ=255)
                                                     tGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAAt                                    <  1:1281558/93‑1 (MQ=255)
                                                       aTTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGt                                  <  2:2241873/93‑1 (MQ=255)
                                                       aTTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGt                                  >  2:3974991/1‑93 (MQ=255)
                                                          cAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCtt                               <  1:6742171/93‑1 (MQ=255)
                                                          cAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCtt                               <  2:4235583/93‑1 (MQ=255)
                                                             gTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTgg                            >  1:5844866/1‑93 (MQ=255)
                                                               cATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTc                          <  1:2876211/93‑1 (MQ=255)
                                                               cATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTc                          <  2:6715470/93‑1 (MQ=255)
                                                                aTACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCGTTTCACCGCAGGCATTAATGTCTTTGGTCt                         >  1:1392645/1‑93 (MQ=255)
                                                                 tACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTg                        >  2:5898473/1‑93 (MQ=255)
                                                                  aCAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTgg                       <  1:6089040/93‑1 (MQ=255)
                                                                  aCAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTgg                       <  2:4428416/93‑1 (MQ=255)
                                                                   cAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTggg                      <  1:3757022/93‑1 (MQ=255)
                                                                     gtgcGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTa                    <  1:4199950/90‑1 (MQ=255)
                                                                      ttCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTag                   >  1:7773005/1‑93 (MQ=255)
                                                                      ttCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTag                   <  2:6474299/93‑1 (MQ=255)
                                                                        cGACACCAAATTCTTTGCAGTTTTTTGATCAGTTCATGCATCGGGATAGGCGCCATTTCACCGCATGCATTAATGTCTTTGGTCTGGGTagag                 <  2:3694608/93‑1 (MQ=255)
                                                                         gACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAgaga                <  1:6972102/93‑1 (MQ=255)
                                                                          aCGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAgagat               >  1:7711957/1‑92 (MQ=255)
                                                                          aCGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAc               >  2:5481301/1‑93 (MQ=255)
                                                                            gCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGt             <  2:5127126/93‑1 (MQ=255)
                                                                             ccAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGtt            <  2:3249469/93‑1 (MQ=255)
                                                                              cAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtg           <  1:2854872/93‑1 (MQ=255)
                                                                              cAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtg           >  1:5434944/1‑93 (MQ=255)
                                                                               aaaTTCTTTGCAGTTTTTTGATCGGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtgt          >  2:1531323/1‑93 (MQ=255)
                                                                                aaTTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtgtg         >  2:852495/1‑93 (MQ=255)
                                                                                 aTTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGa        >  2:7414363/1‑93 (MQ=255)
                                                                                  ttCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGAt       >  2:2861929/1‑93 (MQ=255)
                                                                                   tCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATc      <  2:2327079/93‑1 (MQ=255)
                                                                                     tttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGATGTTGTGATCCa    <  2:4901250/93‑1 (MQ=255)
                                                                                     tttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCa    <  2:6078192/93‑1 (MQ=255)
                                                                                     tttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCa    >  1:3502271/1‑93 (MQ=255)
                                                                                     tttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCa    >  1:4225153/1‑93 (MQ=255)
                                                                                     tttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCa    >  1:4949625/1‑93 (MQ=255)
                                                                                     tttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCa    >  2:1863555/1‑93 (MQ=255)
                                                                                      ttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAt   <  1:4699985/93‑1 (MQ=255)
                                                                                      ttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAt   <  1:4372544/93‑1 (MQ=255)
                                                                                            |                                                                                       
GCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑TTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATG  >  NC_000913/80507‑80685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGT  >  NC_000913/80510‑80696
                                                                                               |                                                                                             
AGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTT                                                                                               <  SRR4302543.2214836/94‑1 (MQ=60)
  GTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTtg                                                                                             <  SRR4302543.2711422/94‑3 (MQ=60)
  GTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTtg                                                                                             <  SRR4302543.340640/94‑3 (MQ=60)
  GTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTtg                                                                                             <  SRR4302543.5597132/94‑3 (MQ=60)
   TGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTtga                                                                                            <  SRR4302543.2217018/94‑4 (MQ=60)
   TGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTtga                                                                                            >  SRR4302543.5720489/1‑91 (MQ=60)
   GGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTtga                                                                                            <  SRR4302543.357051/94‑4 (MQ=60)
     GCGCCCTGGTCCGGCCCCATTTCGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATC                                                                                           <  SRR4302543.2443839/94‑1 (MQ=60)
      CGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCA                                                                                          <  SRR4302543.2375726/94‑1 (MQ=60)
       GCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAG                                                                                         >  SRR4302543.7052268/1‑94 (MQ=60)
       GCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAG                                                                                         >  SRR4302543.3127535/1‑94 (MQ=60)
       GCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAG                                                                                         >  SRR4302543.4778433/1‑94 (MQ=60)
       GCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAG                                                                                         >  SRR4302543.4778209/1‑94 (MQ=60)
       GCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAG                                                                                         >  SRR4302543.3546996/1‑94 (MQ=60)
       GCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAG                                                                                         <  SRR4302543.3646714/94‑1 (MQ=60)
        CCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGT                                                                                        >  SRR4302543.4965032/1‑94 (MQ=60)
        CCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGT                                                                                        >  SRR4302543.4681508/1‑94 (MQ=60)
        CCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGT                                                                                        <  SRR4302543.1949914/94‑1 (MQ=60)
         CCTGTTCCGGCCCCATTACGTGGACGATCCCCTGCTACGGGTGATTCAGGTCATACAGTTGGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTT                                                                                       >  SRR4302543.4075625/1‑94 (MQ=60)
         CCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTT                                                                                       >  SRR4302543.2965619/1‑94 (MQ=60)
         CCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTT                                                                                       >  SRR4302543.2965620/1‑94 (MQ=60)
         CCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTT                                                                                       >  SRR4302543.2723177/1‑94 (MQ=60)
         CCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTT                                                                                       <  SRR4302543.3207129/94‑1 (MQ=60)
         CCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTT                                                                                       <  SRR4302543.7661384/94‑1 (MQ=60)
         CCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTT                                                                                       >  SRR4302543.4075623/1‑94 (MQ=60)
         CCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTT                                                                                       <  SRR4302543.1697751/94‑1 (MQ=60)
          CTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTC                                                                                      >  SRR4302543.7573945/1‑94 (MQ=60)
          CTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTC                                                                                      >  SRR4302543.2894270/1‑94 (MQ=60)
          CTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTC                                                                                      >  SRR4302543.6555426/1‑94 (MQ=60)
           TGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCC                                                                                     <  SRR4302543.3039727/94‑1 (MQ=60)
           TGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCC                                                                                     >  SRR4302543.6673045/1‑94 (MQ=60)
           CGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCC                                                                                     <  SRR4302543.5399678/94‑1 (MQ=60)
             TTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTG                                                                                   >  SRR4302543.7086602/1‑94 (MQ=60)
              TCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGC                                                                                  >  SRR4302543.4409053/1‑94 (MQ=60)
               CCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCA                                                                                 <  SRR4302543.5523198/94‑1 (MQ=60)
               CCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCA                                                                                 >  SRR4302543.7796251/1‑94 (MQ=60)
               CCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCA                                                                                 >  SRR4302543.2223136/1‑94 (MQ=60)
               CCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCA                                                                                 >  SRR4302543.2223366/1‑94 (MQ=60)
                CGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCAT                                                                                >  SRR4302543.2789041/1‑94 (MQ=60)
                 GGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATC                                                                               >  SRR4302543.1204680/1‑94 (MQ=60)
                  GCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCT                                                                              <  SRR4302543.365846/94‑1 (MQ=60)
                  GCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCCTTGCAGTT‑TTTTGATCATTTCCTGCATCT                                                                              >  SRR4302543.808433/1‑94 (MQ=60)
                   CCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTG                                                                             <  SRR4302543.222517/94‑1 (MQ=60)
                   CCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTG                                                                             >  SRR4302543.3148539/1‑94 (MQ=60)
                   CCCCATTACGTGGACGATCCCCTGATACGAGTGATTCAGGTCATACAGTTCGACGCCTAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTG                                                                             <  SRR4302543.4273156/94‑1 (MQ=60)
                    CCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGG                                                                            <  SRR4302543.2793074/94‑1 (MQ=60)
                    CCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGG                                                                            >  SRR4302543.425374/1‑94 (MQ=60)
                    CCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGG                                                                            >  SRR4302543.187858/1‑94 (MQ=60)
                     CCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGA                                                                           >  SRR4302543.126854/1‑94 (MQ=60)
                      CATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGAT                                                                          >  SRR4302543.3088160/1‑94 (MQ=60)
                      CATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGAT                                                                          <  SRR4302543.3079873/94‑1 (MQ=60)
                       ATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATA                                                                         >  SRR4302543.1382552/1‑94 (MQ=60)
                       ATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATA                                                                         >  SRR4302543.4133761/1‑94 (MQ=60)
                        TTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATAC                                                                        <  SRR4302543.7353661/94‑1 (MQ=60)
                         TACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACG                                                                       <  SRR4302543.2489931/94‑1 (MQ=60)
                         TACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACG                                                                       >  SRR4302543.7011319/1‑94 (MQ=60)
                          ACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGC                                                                      >  SRR4302543.1700854/1‑94 (MQ=60)
                          ACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGC                                                                      <  SRR4302543.4177650/94‑1 (MQ=60)
                          ACGTGGACGATCCCCTGATACGGGAGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑TGTTTTGATCAGTTCCTGCATCTGGATACGC                                                                      >  SRR4302543.314153/1‑94 (MQ=60)
                          ACGTGGACGATCCCCTGATACGGCTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGC                                                                      <  SRR4302543.249041/94‑1 (MQ=60)
                           CGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCC                                                                     >  SRR4302543.2712347/1‑94 (MQ=60)
                            GTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGC                                                                    >  SRR4302543.747576/1‑94 (MQ=60)
                            GTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGC                                                                    >  SRR4302543.3583963/1‑94 (MQ=60)
                              GGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCA                                                                  <  SRR4302543.2791636/94‑1 (MQ=60)
                              GGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCA                                                                  >  SRR4302543.4209276/1‑94 (MQ=60)
                                GCGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATT                                                                <  SRR4302543.1035548/94‑1 (MQ=60)
                                ACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATT                                                                <  SRR4302543.5257403/94‑1 (MQ=60)
                                 CGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTT                                                               >  SRR4302543.2723158/1‑94 (MQ=60)
                                  GATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTC                                                              >  SRR4302543.4072996/1‑94 (MQ=60)
                                  GATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTC                                                              >  SRR4302543.6992861/1‑94 (MQ=60)
                                  GATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTC                                                              <  SRR4302543.1710968/94‑1 (MQ=60)
                                  GATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTC                                                              >  SRR4302543.5074841/1‑94 (MQ=60)
                                     CCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACC                                                           <  SRR4302543.6360518/94‑1 (MQ=60)
                                     CCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACC                                                           <  SRR4302543.7337831/94‑1 (MQ=60)
                                      CCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCG                                                          <  SRR4302543.5555013/94‑1 (MQ=60)
                                      CCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCG                                                          >  SRR4302543.2294364/1‑94 (MQ=60)
                                       CCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGC                                                         >  SRR4302543.5264443/1‑94 (MQ=60)
                                       CCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGC                                                         >  SRR4302543.4565683/1‑94 (MQ=60)
                                        CTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCA                                                        <  SRR4302543.5069104/94‑1 (MQ=60)
                                        CTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCA                                                        <  SRR4302543.7160796/94‑1 (MQ=60)
                                        CTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCA                                                        <  SRR4302543.2326703/94‑1 (MQ=60)
                                        CTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCA                                                        <  SRR4302543.4094007/94‑1 (MQ=60)
                                         TGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTGCTGCATCTGGATACGCGCCATTTCACCGCAG                                                       >  SRR4302543.7579071/1‑94 (MQ=60)
                                         TGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAG                                                       <  SRR4302543.994859/94‑1 (MQ=60)
                                         TGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAG                                                       <  SRR4302543.40241/94‑1 (MQ=60)
                                         TGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAG                                                       >  SRR4302543.2284568/1‑94 (MQ=60)
                                          GATACGTGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCACG                                                      <  SRR4302543.2419605/94‑1 (MQ=60)
                                          GATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGG                                                      <  SRR4302543.4765304/94‑1 (MQ=60)
                                          GATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGG                                                      <  SRR4302543.5840405/94‑1 (MQ=60)
                                          GATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGG                                                      >  SRR4302543.162218/1‑94 (MQ=60)
                                           ATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGC                                                     >  SRR4302543.5147975/1‑94 (MQ=60)
                                           ATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGC                                                     <  SRR4302543.7880422/94‑1 (MQ=60)
                                             ACGGGTGATTCAGGTCACACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATT                                                  <  SRR4302543.2077900/94‑1 (MQ=60)
                                              CGGGTGATTCAGGTCATACCGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATT                                                  >  SRR4302543.1807061/1‑94 (MQ=60)
                                              CGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATT                                                  >  SRR4302543.125198/1‑94 (MQ=60)
                                              CGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATT                                                  <  SRR4302543.4383853/94‑1 (MQ=60)
                                              CGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATT                                                  <  SRR4302543.7430574/94‑1 (MQ=60)
                                               GGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTA                                                 >  SRR4302543.808779/1‑94 (MQ=60)
                                               GGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTA                                                 >  SRR4302543.2435608/1‑94 (MQ=60)
                                                 GTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAAT                                               <  SRR4302543.7368256/94‑1 (MQ=60)
                                                 GTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAAT                                               <  SRR4302543.1840029/94‑1 (MQ=60)
                                                  TGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATG                                              <  SRR4302543.1292986/94‑1 (MQ=60)
                                                  TGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATG                                              <  SRR4302543.3377241/94‑1 (MQ=60)
                                                   TATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGT                                             <  SRR4302543.4258609/94‑1 (MQ=60)
                                                   GATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGT                                             <  SRR4302543.6566934/94‑1 (MQ=60)
                                                    ATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTC                                            >  SRR4302543.6144848/1‑94 (MQ=60)
                                                    ATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTC                                            >  SRR4302543.6144846/1‑94 (MQ=60)
                                                    ATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTC                                            >  SRR4302543.4292015/1‑94 (MQ=60)
                                                     TTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCT                                           >  SRR4302543.4825501/1‑94 (MQ=60)
                                                       CAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTT                                         <  SRR4302543.6805838/94‑1 (MQ=60)
                                                       CAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTT                                         <  SRR4302543.1982526/94‑1 (MQ=60)
                                                        AGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTG                                        <  SRR4302543.6165004/94‑1 (MQ=60)
                                                        AGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTG                                        >  SRR4302543.1811077/1‑94 (MQ=60)
                                                        AGGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTG                                        <  SRR4302543.7024303/94‑1 (MQ=60)
                                                         GGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGG                                       >  SRR4302543.379940/1‑94 (MQ=60)
                                                         GGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGG                                       >  SRR4302543.5729824/1‑94 (MQ=60)
                                                         GGTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGG                                       >  SRR4302543.5898045/1‑94 (MQ=60)
                                                          GTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGT                                      >  SRR4302543.6552951/1‑94 (MQ=60)
                                                          GTCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGT                                      <  SRR4302543.1534579/94‑1 (MQ=60)
                                                           TCATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTC                                     <  SRR4302543.5437842/94‑1 (MQ=60)
                                                            CATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCGTTTCACCGCAGGCATTAATGTCTTTGGTCT                                    >  SRR4302543.1405184/1‑94 (MQ=60)
                                                            CATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCT                                    <  SRR4302543.2902387/94‑1 (MQ=60)
                                                            CATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCT                                    >  SRR4302543.1625763/1‑94 (MQ=60)
                                                            CATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCT                                    <  SRR4302543.1227880/94‑1 (MQ=60)
                                                            CATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCT                                    <  SRR4302543.6569449/94‑1 (MQ=60)
                                                             ATACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTG                                   >  SRR4302543.7306555/1‑94 (MQ=60)
                                                               ACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGG                                 <  SRR4302543.5893210/94‑1 (MQ=60)
                                                               ACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGG                                 <  SRR4302543.6951307/94‑1 (MQ=60)
                                                               ACAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGG                                 <  SRR4302543.6146750/94‑1 (MQ=60)
                                                                CAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGT                                >  SRR4302543.5895500/1‑94 (MQ=60)
                                                                CAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGT                                >  SRR4302543.2530313/1‑94 (MQ=60)
                                                                CAGTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGT                                <  SRR4302543.3790080/94‑1 (MQ=60)
                                                                  GTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAG                              <  SRR4302543.6517039/94‑1 (MQ=60)
                                                                  GTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAG                              >  SRR4302543.1313435/1‑94 (MQ=60)
                                                                  GTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAG                              <  SRR4302543.1472802/94‑1 (MQ=60)
                                                                  GTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAG                              >  SRR4302543.3605/1‑94 (MQ=60)
                                                                  GTTCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAG                              >  SRR4302543.7846323/1‑94 (MQ=60)
                                                                  GTGCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAG                              <  SRR4302543.4238028/94‑1 (MQ=60)
                                                                    TCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAG                            <  SRR4302543.1762754/94‑1 (MQ=60)
                                                                    TCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAG                            <  SRR4302543.5051397/94‑1 (MQ=60)
                                                                    GCGACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAG                            <  SRR4302543.2958081/94‑1 (MQ=60)
                                                                      GACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAT                          >  SRR4302543.7784905/1‑94 (MQ=60)
                                                                      GACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAC                          >  SRR4302543.7785060/1‑94 (MQ=60)
                                                                      GACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAC                          >  SRR4302543.5880549/1‑94 (MQ=60)
                                                                      GACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAC                          <  SRR4302543.7037713/94‑1 (MQ=60)
                                                                      GACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAC                          <  SRR4302543.7037710/94‑1 (MQ=60)
                                                                      GACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAC                          >  SRR4302543.4968679/1‑94 (MQ=60)
                                                                       ACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACG                         >  SRR4302543.6980171/1‑94 (MQ=60)
                                                                       ACGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACG                         >  SRR4302543.2153546/1‑94 (MQ=60)
                                                                        CGCCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGT                        >  SRR4302543.7916033/1‑94 (MQ=60)
                                                                          CCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTG                      >  SRR4302543.307201/1‑94 (MQ=60)
                                                                          CCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTG                      >  SRR4302543.2067458/1‑94 (MQ=60)
                                                                          CCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTG                      >  SRR4302543.5484314/1‑94 (MQ=60)
                                                                          CCAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTG                      >  SRR4302543.55839/1‑94 (MQ=60)
                                                                           CAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGT                     >  SRR4302543.3644596/1‑94 (MQ=60)
                                                                           CAAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGT                     <  SRR4302543.2880917/94‑1 (MQ=60)
                                                                            AAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTG                    >  SRR4302543.4626380/1‑94 (MQ=60)
                                                                            AAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTG                    >  SRR4302543.2893371/1‑94 (MQ=60)
                                                                            AAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTG                    <  SRR4302543.4284817/94‑1 (MQ=60)
                                                                            AAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTG                    <  SRR4302543.1733954/94‑1 (MQ=60)
                                                                            AAATTCTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTG                    >  SRR4302543.2192916/1‑94 (MQ=60)
                                                                                 CTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCA               >  SRR4302543.3789751/1‑94 (MQ=60)
                                                                                 CTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCA               >  SRR4302543.3964675/1‑94 (MQ=60)
                                                                                 CTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCA               >  SRR4302543.3533591/1‑94 (MQ=60)
                                                                                 CTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCA               >  SRR4302543.4263467/1‑94 (MQ=60)
                                                                                 CTTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCA               >  SRR4302543.4994693/1‑94 (MQ=60)
                                                                                  TTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAT              <  SRR4302543.3258628/94‑1 (MQ=60)
                                                                                  TTTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAT              >  SRR4302543.775144/1‑94 (MQ=60)
                                                                                   TTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATG             <  SRR4302543.4742908/94‑1 (MQ=60)
                                                                                   TTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATG             <  SRR4302543.4412143/94‑1 (MQ=60)
                                                                                   TTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATG             >  SRR4302543.6372799/1‑94 (MQ=60)
                                                                                   TTGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATG             <  SRR4302543.4412144/94‑1 (MQ=60)
                                                                                    TGCAGTT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGG            <  SRR4302543.1568116/94‑1 (MQ=60)
                                                                                      CAGTT‑TTTTGATCAGTCCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTA          <  SRR4302543.6919464/94‑1 (MQ=60)
                                                                                       agtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAG         >  SRR4302543.6742902/4‑94 (MQ=60)
                                                                                        gtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGC        >  SRR4302543.1536329/3‑94 (MQ=60)
                                                                                        gtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGC        >  SRR4302543.7837507/3‑94 (MQ=60)
                                                                                        gtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGC        >  SRR4302543.3708352/3‑94 (MQ=60)
                                                                                        gtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGC        >  SRR4302543.6609730/3‑94 (MQ=60)
                                                                                        gtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGC        >  SRR4302543.4607518/3‑94 (MQ=60)
                                                                                        gtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGC        >  SRR4302543.5797107/3‑94 (MQ=60)
                                                                                        gtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGC        <  SRR4302543.5015023/92‑1 (MQ=60)
                                                                                        gtT‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGAATTAATGTCTTTGGTatggggagaggttttgataccaatgagcgc        >  SRR4302543.6636167/3‑64 (MQ=60)
                                                                                        T‑T‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCG       >  SRR4302543.916239/1‑94 (MQ=60)
                                                                                        T‑T‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCG       <  SRR4302543.7171228/94‑1 (MQ=60)
                                                                                          T‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGA      <  SRR4302543.3467557/94‑1 (MQ=60)
                                                                                          T‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGA      >  SRR4302543.7632632/1‑94 (MQ=60)
                                                                                             TTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAG    <  SRR4302543.3276669/94‑1 (MQ=60)
                                                                                             TTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAG    <  SRR4302543.7058291/94‑1 (MQ=60)
                                                                                             TTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAG    <  SRR4302543.176650/94‑1 (MQ=60)
                                                                                              TTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGG   <  SRR4302543.4342963/94‑1 (MQ=60)
                                                                                              TTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGG   <  SRR4302543.4418099/94‑1 (MQ=60)
                                                                                              TTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGG   <  SRR4302543.3356025/94‑1 (MQ=60)
                                                                                               TGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGT  <  SRR4302543.5242765/94‑1 (MQ=60)
                                                                                               TGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGT  >  SRR4302543.128204/1‑94 (MQ=60)
                                                                                               TGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGT  >  SRR4302543.5224909/1‑94 (MQ=60)
                                                                                               TGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGT  <  SRR4302543.2013096/94‑1 (MQ=60)
                                                                                               |                                                                                             
AGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑T‑TTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGT  >  NC_000913/80510‑80696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑