Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,964,115	G→A	S34L (TCA→TTA)	flhB ←	FlhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,964,115	0	G	A	99.5%	768.8 / NA	214	S34L (TCA→TTA)	flhB	FlhB
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (106/107); minor base C (1/0); total (107/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

AGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTGAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTC  >  NC_000913/1964025‑1964204

aGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAg                                                                                         <  1:3961533/93‑1 (MQ=255)

aGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAg                                                                                         <  1:5280031/93‑1 (MQ=255)

aGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAg                                                                                         <  2:457757/93‑1 (MQ=255)

aGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAg                                                                                         <  1:2306811/93‑1 (MQ=255)

 gCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAgg                                                                                        >  1:4885751/1‑93 (MQ=255)

 gCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAgg                                                                                        <  2:138536/93‑1 (MQ=255)

  cccAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCCACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAGATCAGCAGTAAGGt                                                                                       <  2:82212/93‑1 (MQ=255)

      gCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGt                                                                                   >  2:6917040/1‑93 (MQ=255)

        ggAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTc                                                                                 >  2:6191187/1‑93 (MQ=255)

          agagCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCAc                                                                               <  2:5747304/93‑1 (MQ=255)

           aagCATGCCCGACAATCGACGGGCCAGCGACACGCCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACg                                                                              <  2:4904522/92‑1 (MQ=255)

            agCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACgg                                                                             >  2:3784692/1‑93 (MQ=255)

            agCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACgg                                                                             <  2:12467/93‑1 (MQ=255)

            agCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACgg                                                                             <  1:1337263/93‑1 (MQ=255)

               aTGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGaa                                                                          <  1:2692229/93‑1 (MQ=255)

               aTGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGaa                                                                          <  2:1702391/93‑1 (MQ=255)

               aTGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGaa                                                                          <  1:594397/93‑1 (MQ=255)

               aTGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGaa                                                                          <  1:4450060/93‑1 (MQ=255)

                tGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAc                                                                         >  2:2910474/1‑93 (MQ=255)

                tGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAc                                                                         >  1:1160042/1‑93 (MQ=255)

                tGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAc                                                                         >  1:2455244/1‑93 (MQ=255)

                tGCCCGACAATCGACGGGCCAGAGACACACCGCCAAACCAGATAACACTAAAGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGAGAAc                                                                         >  2:1021835/1‑93 (MQ=255)

                ggCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAc                                                                         <  2:5347842/92‑1 (MQ=255)

                  cccGACAATCGACGGGCCAGCGACACGCCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAcgc                                                                       <  1:3031405/93‑1 (MQ=255)

                  cccGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCATTTCACGGGAAcgc                                                                       <  2:1577385/93‑1 (MQ=255)

                   ccGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAcgcg                                                                      >  1:7141702/1‑93 (MQ=255)

                    cGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCgg                                                                     <  2:3353044/93‑1 (MQ=255)

                    cGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCgg                                                                     >  2:790049/1‑93 (MQ=255)

                    cGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGGAAGGTCAGTTCACGGGAACGCgg                                                                     >  2:4331207/1‑93 (MQ=255)

                      aCAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGa                                                                   >  1:776164/1‑93 (MQ=255)

                      aCAACCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGa                                                                   <  2:7139938/93‑1 (MQ=255)

                       cAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGAt                                                                  <  2:4806759/93‑1 (MQ=255)

                       cAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGAt                                                                  >  2:1042423/1‑93 (MQ=255)

                        aaTCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGAtt                                                                 <  1:3610699/93‑1 (MQ=255)

                         aTCGACGGGCCAGCGCCACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGAttt                                                                <  2:3426284/93‑1 (MQ=255)

                          tCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTg                                                               >  1:5348053/1‑93 (MQ=255)

                          tCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTg                                                               >  2:3258752/1‑93 (MQ=255)

                               gggCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCct                                                          >  1:3114235/1‑93 (MQ=255)

                               gggCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCct                                                          >  2:6488982/1‑93 (MQ=255)

                                ggCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCctc                                                         <  1:196623/93‑1 (MQ=255)

                                ggCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCctc                                                         <  2:2764519/93‑1 (MQ=255)

                                 gCCAGCGACACACCGCGAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCctct                                                        <  1:1011752/93‑1 (MQ=255)

                                 gCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCctct                                                        <  1:7645992/93‑1 (MQ=255)

                                   cAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTc                                                      >  1:4571681/1‑93 (MQ=255)

                                   cAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTc                                                      <  2:3037957/93‑1 (MQ=255)

                                     gCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTccc                                                    <  1:5452927/93‑1 (MQ=255)

                                       gACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCcgc                                                  <  1:7686542/93‑1 (MQ=255)

                                       gACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCcgc                                                  >  2:6910302/1‑93 (MQ=255)

                                          acacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCt                                               >  2:7087564/1‑93 (MQ=255)

                                          acacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCt                                               >  1:7086275/1‑93 (MQ=255)

                                          acacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCt                                               >  1:3185825/1‑93 (MQ=255)

                                          acacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCt                                               >  2:4045550/1‑93 (MQ=255)

                                           cacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCtt                                              >  2:5969188/1‑93 (MQ=255)

                                           cacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCtt                                              >  1:286415/1‑93 (MQ=255)

                                            cccGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCttt                                             <  1:1670041/92‑1 (MQ=255)

                                             ccGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCtttt                                            <  2:6127110/93‑1 (MQ=255)

                                             acGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCGCTCTTCCCGCGCtttt                                            >  2:2491085/2‑93 (MQ=255)

                                               gCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTc                                          <  2:2602055/93‑1 (MQ=255)

                                               gCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTc                                          >  1:6589423/1‑93 (MQ=255)

                                                 cAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTa                                        <  1:451054/93‑1 (MQ=255)

                                                   aaCCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGt                                      >  2:4130151/1‑93 (MQ=255)

                                                    aCCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTc                                     <  1:6057334/93‑1 (MQ=255)

                                                     ccAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCg                                    >  2:1112664/1‑93 (MQ=255)

                                                      cAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCgg                                   <  1:2968060/93‑1 (MQ=255)

                                                      cAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCgg                                   <  2:6407141/93‑1 (MQ=255)

                                                      cAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCgg                                   >  1:7238115/1‑93 (MQ=255)

                                                       aGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCggg                                  >  1:3559214/1‑92 (MQ=255)

                                                       aGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGt                                  >  2:4082394/1‑93 (MQ=255)

                                                       aGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGt                                  <  2:2417076/93‑1 (MQ=255)

                                                            acacTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgt                             <  1:4280503/93‑1 (MQ=255)

                                                            acacTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgt                             <  1:7699515/93‑1 (MQ=255)

                                                             cacTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgtg                            >  2:7325080/1‑93 (MQ=255)

                                                              acTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgtgt                           >  1:3253335/1‑93 (MQ=255)

                                                              acTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgtgt                           <  2:6764778/93‑1 (MQ=255)

                                                               cTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGtgtg                          >  2:6833742/1‑93 (MQ=255)

                                                               cTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGtgtg                          <  2:637235/93‑1 (MQ=255)

                                                               cTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGtgtg                          >  1:1457795/1‑93 (MQ=255)

                                                                tAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTgg                         <  2:5487213/93‑1 (MQ=255)

                                                                tAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTgg                         <  1:7456702/93‑1 (MQ=255)

                                                                 aaCGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTggg                        <  1:7303592/93‑1 (MQ=255)

                                                                  aCGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTgggg                       <  1:4684398/93‑1 (MQ=255)

                                                                   cGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGTATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTggggg                      <  2:5321351/93‑1 (MQ=255)

                                                                    gCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGc                     <  2:561991/93‑1 (MQ=255)

                                                                     cccACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCt                    >  2:5252492/1‑93 (MQ=255)

                                                                      ccaccaGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCtt                   >  1:5191477/1‑93 (MQ=255)

                                                                       caccaGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTc                  >  1:4418871/1‑93 (MQ=255)

                                                                        accaGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCTGTGAGGTGTGGGGGCTTCt                 >  1:5699813/1‑93 (MQ=255)

                                                                           aGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGtt              >  1:675445/1‑93 (MQ=255)

                                                                            gCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGttt             >  1:4115649/1‑93 (MQ=255)

                                                                             cAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGtttt            >  2:7640005/1‑93 (MQ=255)

                                                                              aaaaTCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTg           >  1:4955007/1‑93 (MQ=255)

                                                                               aaaTCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTgt          >  2:2385649/1‑93 (MQ=255)

                                                                               aaaTCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTgt          <  2:1911698/93‑1 (MQ=255)

                                                                               aaaTCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTgt          <  1:2656649/93‑1 (MQ=255)

                                                                                  tCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTgtcgt       <  2:7278915/93‑1 (MQ=255)

                                                                                      cagTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGct   <  1:2375394/93‑1 (MQ=255)

                                                                                       agTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGctc  <  1:5521695/93‑1 (MQ=255)

                                                                                       agTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGctc  <  1:559653/93‑1 (MQ=255)

                                                                                       agTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGctc  <  1:5959816/93‑1 (MQ=255)

                                                                                       agTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGctc  <  1:2103543/93‑1 (MQ=255)

AGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTGAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTC  >  NC_000913/1964025‑1964204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTGAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTCGT  >  NC_000913/1964022‑1964208

TGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTA                                                                                               <  SRR4302543.179212/94‑1 (MQ=60)

 GCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAA                                                                                              <  SRR4302543.3940676/94‑1 (MQ=60)

 GCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAA                                                                                              >  SRR4302543.4626839/1‑94 (MQ=60)

 GCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAA                                                                                              <  SRR4302543.7855393/94‑1 (MQ=60)

  CAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAG                                                                                             <  SRR4302543.2605711/94‑1 (MQ=60)

  CAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAG                                                                                             <  SRR4302543.6031020/94‑1 (MQ=60)

  CAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAG                                                                                             <  SRR4302543.6834568/94‑1 (MQ=60)

   AGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGG                                                                                            <  SRR4302543.3996618/94‑1 (MQ=60)

   AGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGG                                                                                            >  SRR4302543.4930285/1‑94 (MQ=60)

   AGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGG                                                                                            <  SRR4302543.2327819/94‑1 (MQ=60)

   AGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGG                                                                                            <  SRR4302543.5327674/94‑1 (MQ=60)

      CCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCA                                                                                         >  SRR4302543.5347168/1‑94 (MQ=60)

       CAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAG                                                                                        >  SRR4302543.3584089/1‑94 (MQ=60)

       CAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAG                                                                                        <  SRR4302543.2253940/94‑1 (MQ=60)

        AGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGT                                                                                       <  SRR4302543.488382/94‑1 (MQ=60)

           GGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCA                                                                                    >  SRR4302543.2771983/1‑94 (MQ=60)

           GGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCA                                                                                    >  SRR4302543.4548996/1‑94 (MQ=60)

              gagcaaggcacaaaagcggCGGGCCAACGATACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTGAGGTCAGTTCACGG                                                                                 <  SRR4302543.2574271/75‑1 (MQ=60)

               AGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCACACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGG                                                                                >  SRR4302543.5557850/1‑94 (MQ=60)

               AGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTCAGGTCAGTTCACGGG                                                                                >  SRR4302543.6709973/1‑94 (MQ=60)

               AGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGG                                                                                <  SRR4302543.1349194/94‑1 (MQ=60)

               AGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGG                                                                                <  SRR4302543.1349192/94‑1 (MQ=60)

                GCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGA                                                                               >  SRR4302543.1232132/1‑94 (MQ=60)

                 CATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAA                                                                              >  SRR4302543.4025559/1‑94 (MQ=60)

                  ATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAC                                                                             >  SRR4302543.1170608/1‑94 (MQ=60)

                  ATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAC                                                                             >  SRR4302543.2477388/1‑94 (MQ=60)

                  ATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAC                                                                             <  SRR4302543.4490363/94‑1 (MQ=60)

                  ATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAC                                                                             <  SRR4302543.2716637/94‑1 (MQ=60)

                  ATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAC                                                                             <  SRR4302543.4490364/94‑1 (MQ=60)

                  ATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAC                                                                             <  SRR4302543.599850/94‑1 (MQ=60)

                   TGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACG                                                                            <  SRR4302543.3075073/94‑1 (MQ=60)

                     CCCGACAATCGACGGGCCAGCGACACGCCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCG                                                                          <  SRR4302543.3058779/94‑1 (MQ=60)

                     CCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCG                                                                          >  SRR4302543.7211100/1‑94 (MQ=60)

                      CCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGG                                                                         >  SRR4302543.2803781/1‑94 (MQ=60)

                       CGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCCCCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGG                                                                        >  SRR4302543.468346/1‑94 (MQ=60)

                        GACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGA                                                                       >  SRR4302543.782984/1‑94 (MQ=60)

                           AATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTT                                                                    <  SRR4302543.3642545/94‑1 (MQ=60)

                            ATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTG                                                                   <  SRR4302543.6010089/94‑1 (MQ=60)

                            ATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTG                                                                   >  SRR4302543.493208/1‑94 (MQ=60)

                            ATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTG                                                                   >  SRR4302543.5396450/1‑94 (MQ=60)

                                 CGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCT                                                              <  SRR4302543.3376012/94‑1 (MQ=60)

                                 CGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCT                                                              <  SRR4302543.6256954/94‑1 (MQ=60)

                                 CGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCT                                                              >  SRR4302543.3142248/1‑94 (MQ=60)

                                   GGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCT                                                            <  SRR4302543.198284/94‑1 (MQ=60)

                                   GGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCT                                                            <  SRR4302543.3618096/94‑1 (MQ=60)

                                    GCCAGCGACACACCGCGAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTT                                                           <  SRR4302543.1020701/94‑1 (MQ=60)

                                    GCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTT                                                           >  SRR4302543.5080792/1‑94 (MQ=60)

                                    GCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTT                                                           <  SRR4302543.7718497/94‑1 (MQ=60)

                                    GCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTT                                                           <  SRR4302543.726360/94‑1 (MQ=60)

                                     CCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTC                                                          >  SRR4302543.800494/1‑94 (MQ=60)

                                     CCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTC                                                          >  SRR4302543.4613253/1‑94 (MQ=60)

                                      CAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCC                                                         <  SRR4302543.2710811/94‑1 (MQ=60)

                                       AGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCC                                                        >  SRR4302543.294291/1‑94 (MQ=60)

                                       AGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGACCCTCTTCCC                                                        >  SRR4302543.6093665/1‑94 (MQ=60)

                                        GCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCG                                                       <  SRR4302543.5502372/94‑1 (MQ=60)

                                         CGACACAGCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGC                                                      >  SRR4302543.7824487/1‑94 (MQ=60)

                                          GACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCG                                                     >  SRR4302543.5239447/1‑94 (MQ=60)

                                          GACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCG                                                     <  SRR4302543.7759333/94‑1 (MQ=60)

                                            CACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCT                                                   >  SRR4302543.7155093/1‑94 (MQ=60)

                                            CACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCT                                                   >  SRR4302543.3214558/1‑94 (MQ=60)

                                            CACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCT                                                   >  SRR4302543.3214559/1‑94 (MQ=60)

                                            CACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCT                                                   >  SRR4302543.561492/1‑94 (MQ=60)

                                             ACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTT                                                  >  SRR4302543.288964/1‑94 (MQ=60)

                                              CACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTT                                                 >  SRR4302543.5931312/1‑94 (MQ=60)

                                               CCCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTT                                                <  SRR4302543.1685009/94‑1 (MQ=60)

                                                 CGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTC                                              >  SRR4302543.6651505/1‑94 (MQ=60)

                                                   CCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTA                                            >  SRR4302543.5697946/1‑94 (MQ=60)

                                                    CAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTct                                           >  SRR4302543.1604121/1‑92 (MQ=60)

                                                    CAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAG                                           <  SRR4302543.455267/94‑1 (MQ=60)

                                                    CAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAG                                           <  SRR4302543.7517105/94‑1 (MQ=60)

                                                    CAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAG                                           <  SRR4302543.7197510/94‑1 (MQ=60)

                                                     AAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGT                                          >  SRR4302543.2078120/1‑94 (MQ=60)

                                                       ACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCG                                        <  SRR4302543.6114678/94‑1 (MQ=60)

                                                        CCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGG                                       >  SRR4302543.7308188/1‑94 (MQ=60)

                                                        CCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGG                                       >  SRR4302543.2995415/1‑94 (MQ=60)

                                                         CAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGT                                      <  SRR4302543.2995012/94‑1 (MQ=60)

                                                         CAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGG                                      >  SRR4302543.3590737/1‑94 (MQ=60)

                                                          AGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTG                                     >  SRR4302543.2671744/1‑94 (MQ=60)

                                                           GATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGA                                    >  SRR4302543.2402786/1‑94 (MQ=60)

                                                            ATAACNCTAANGCCCACCAGCNANATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAG                                   <  SRR4302543.7323890/94‑1 (MQ=60)

                                                             TAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGG                                  <  SRR4302543.2083780/94‑1 (MQ=60)

                                                               ACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTG                                <  SRR4302543.4319405/94‑1 (MQ=60)

                                                               ACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTG                                <  SRR4302543.3176494/94‑1 (MQ=60)

                                                               ACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTG                                <  SRR4302543.7772388/94‑1 (MQ=60)

                                                                CACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGT                               >  SRR4302543.3282749/1‑94 (MQ=60)

                                                                CACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGT                               <  SRR4302543.2386013/94‑1 (MQ=60)

                                                                 ACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTG                              >  SRR4302543.1470949/1‑94 (MQ=60)

                                                                  CTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGG                             <  SRR4302543.4448626/94‑1 (MQ=60)

                                                                  CTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGG                             <  SRR4302543.7564462/94‑1 (MQ=60)

                                                                  CTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGG                             >  SRR4302543.1727451/1‑94 (MQ=60)

                                                                  ATAACGCCCACCAGCAAAATCAGCAGTAAGGTCATTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGG                             <  SRR4302543.3842101/94‑1 (MQ=60)

                                                                   TAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGG                            <  SRR4302543.7528136/94‑1 (MQ=60)

                                                                    AACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGG                           <  SRR4302543.7374030/94‑1 (MQ=60)

                                                                    AACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGG                           <  SRR4302543.1156048/94‑1 (MQ=60)

                                                                     ACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGG                          <  SRR4302543.4727132/94‑1 (MQ=60)

                                                                      CGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGC                         >  SRR4302543.4606307/1‑94 (MQ=60)

                                                                      CGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGC                         >  SRR4302543.6943230/1‑94 (MQ=60)

                                                                       GACCAACAGCAAAGTCAGCAGTATGGTCAGTTCACGGGAACGCGGGAATTGCCCCTCTTCCCGAGCTTATTCTAGTCGGTGAGGTGTGGGGACT                        <  SRR4302543.7094579/94‑1 (MQ=60)

                                                                        CCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTT                       >  SRR4302543.5238290/1‑94 (MQ=60)

                                                                         CCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTC                      >  SRR4302543.4458921/1‑94 (MQ=60)

                                                                         CCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTC                      >  SRR4302543.4458923/1‑94 (MQ=60)

                                                                          CACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCTGTGAGGTGTGGGGGCTTCT                     >  SRR4302543.5751804/1‑94 (MQ=60)

                                                                             CAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTT                  >  SRR4302543.681660/1‑94 (MQ=60)

                                                                              AGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTT                 >  SRR4302543.4152723/1‑94 (MQ=60)

                                                                              AGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTT                 >  SRR4302543.5718449/1‑94 (MQ=60)

                                                                                CAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTG               >  SRR4302543.5000127/1‑94 (MQ=60)

                                                                                 AAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGT              >  SRR4302543.5109025/1‑94 (MQ=60)

                                                                                  AAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTC             <  SRR4302543.2680734/94‑1 (MQ=60)

                                                                                   AATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCG            <  SRR4302543.5496266/94‑1 (MQ=60)

                                                                                    ATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGT           <  SRR4302543.4286871/94‑1 (MQ=60)

                                                                                     TCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTC          <  SRR4302543.7729674/94‑1 (MQ=60)

                                                                                     ACAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTC          <  SRR4302543.7771010/94‑1 (MQ=60)

                                                                                         CAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTC      >  SRR4302543.7731538/1‑94 (MQ=60)

                                                                                         CAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTC      <  SRR4302543.2396915/94‑1 (MQ=60)

                                                                                          AGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCT     >  SRR4302543.4770965/1‑94 (MQ=60)

                                                                                          AGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCT     <  SRR4302543.5571852/94‑1 (MQ=60)

                                                                                          AGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCT     <  SRR4302543.2122365/94‑1 (MQ=60)

                                                                                          AGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCT     <  SRR4302543.564780/94‑1 (MQ=60)

                                                                                          AGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCT     <  SRR4302543.6014373/94‑1 (MQ=60)

                                                                                           GTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTC    >  SRR4302543.1993264/1‑94 (MQ=60)

                                                                                           GTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTC    >  SRR4302543.2767418/1‑94 (MQ=60)

                                                                                            TAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTCG   >  SRR4302543.583929/1‑94 (MQ=60)

                                                                                             AAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTCGT  <  SRR4302543.4282876/94‑1 (MQ=60)

                                                                                             AAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTCGT  >  SRR4302543.5086622/1‑94 (MQ=60)

                                                                                             AAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTCGT  <  SRR4302543.6328252/94‑1 (MQ=60)

                                                                                             AAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTCGC  >  SRR4302543.2673442/1‑94 (MQ=60)

TGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTGAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTCTCGT  >  NC_000913/1964022‑1964208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`