Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,033	(C)_5→6	coding (1149/7152 nt)	b1978 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,028	1	.	C	93.2%	519.5 / 30.1	176	coding (1144/7152 nt)	b1978	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/8); new base C (106/58); total (110/66)
Fisher's exact test for biased strand distribution p-value = 5.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 212 total aligned reads displayed.

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT  >  NC_000913/2043936‑2044115

tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACtat‑a                                                                                            >  2:1331614/1‑93 (MQ=255)

tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACtat‑a                                                                                            >  2:7870205/1‑93 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c                                                                                          <  2:1943135/93‑1 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c                                                                                          <  2:3767986/93‑1 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c                                                                                          <  1:2945117/93‑1 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c                                                                                          >  1:2971691/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑c                                                                                        >  1:1568702/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑c                                                                                        >  2:7139004/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑c                                                                                        <  1:7072797/93‑1 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑c                                                                                     >  2:5895758/1‑93 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑c                                                                                     >  2:7006129/1‑93 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑c                                                                                     >  1:7334194/1‑93 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑c                                                                                     >  2:4272559/1‑93 (MQ=255)

     gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑cc                                                                                    >  2:4091847/1‑93 (MQ=255)

       aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑ccct                                                                                  >  2:467867/1‑91 (MQ=255)

       aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑ccct                                                                                  >  1:5200933/1‑91 (MQ=255)

         aGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑cccttc                                                                                <  2:3406473/93‑5 (MQ=255)

         aGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑cccttc                                                                                <  1:1062838/93‑5 (MQ=255)

           tattatGGCGATGAAGTTGCCCTGTTCGATAAAGACGATCGGCAAAGCAATCCTCATGCCATAACCGCCGGACTTAAATAT‑A‑C‑CCACCTTccc                                                                               >  2:2464175/1‑93 (MQ=255)

            attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCg                                                                              <  2:7922147/93‑1 (MQ=255)

             ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGc                                                                             >  2:6234896/1‑93 (MQ=255)

              tatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCt                                                                            <  1:6972486/93‑1 (MQ=255)

              tatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCt                                                                            <  1:7791856/93‑1 (MQ=255)

               atGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCtg                                                                           >  2:6904584/1‑93 (MQ=255)

                tGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCtga                                                                          >  1:2215535/1‑93 (MQ=255)

                    gATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGAc                                                                      >  1:6500656/1‑93 (MQ=255)

                     aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATCC‑C‑CC‑CCTTCCCGCTGATGAcc                                                                     <  2:141841/93‑1 (MQ=255)

                         aGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCa                                                                 >  2:7307562/1‑93 (MQ=255)

                            ggCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAgcg                                                              <  1:39198/93‑1 (MQ=255)

                             gCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAgcgc                                                             <  1:4573109/93‑1 (MQ=255)

                               ccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCgg                                                           >  1:187833/1‑93 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGa                                                          <  2:2806609/93‑1 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGa                                                          >  1:6607194/1‑93 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGa                                                          >  1:5836723/1‑93 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGa                                                          >  2:4992776/1‑93 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         >  1:1621718/1‑93 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         >  1:6316376/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  2:3557882/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  2:6692699/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  2:493544/1‑93 (MQ=255)

                                     cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAAc                                                     <  2:6570148/93‑1 (MQ=255)

                                     cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAAc                                                     >  2:4811409/1‑93 (MQ=255)

                                      gATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                    >  1:4284406/1‑93 (MQ=255)

                                      gATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                    >  1:7467200/1‑93 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATCC‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                   <  2:4018661/93‑1 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                   >  1:3401772/1‑93 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                   >  1:962045/1‑93 (MQ=255)

                                            gACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggg                                              >  2:1372249/1‑93 (MQ=255)

                                            gACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggg                                              >  1:6044053/1‑93 (MQ=255)

                                             aCGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggga                                             >  1:6080055/1‑92 (MQ=255)

                                              cGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTa                                            <  1:5388123/93‑1 (MQ=255)

                                                aTCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTaaa                                          <  2:5745143/93‑1 (MQ=255)

                                                  cgcgaaaGTAATCCTCATGCCATAACCGCTGGACTTAACTATGC‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACa                                        <  2:299877/89‑1 (MQ=255)

                                                  cGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACa                                        <  2:5600766/93‑1 (MQ=255)

                                                  cGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCACGGTAAACa                                        >  1:4010256/1‑93 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                       >  2:3980051/1‑93 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTGCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                       <  1:7608908/93‑1 (MQ=255)

                                                    gCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAgg                                      <  2:3923613/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    >  1:5242290/1‑93 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    >  2:2412798/1‑93 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    <  2:7516893/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    >  1:3710901/1‑93 (MQ=255)

                                                        aGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGa                                  >  2:1863710/1‑93 (MQ=255)

                                                         gTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaa                                 >  2:581832/1‑93 (MQ=255)

                                                          tAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaa                                <  1:1111387/93‑1 (MQ=255)

                                                          tAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaa                                >  2:60200/1‑93 (MQ=255)

                                                          tAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaa                                >  1:2496134/1‑93 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               <  1:2100207/93‑1 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               >  1:7477029/1‑93 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               <  1:7422832/93‑1 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               <  1:5499190/93‑1 (MQ=255)

                                                            aTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAt                              <  2:6422993/93‑1 (MQ=255)

                                                             tCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATg                             >  1:7358677/1‑93 (MQ=255)

                                                             tCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATg                             >  1:2876582/1‑93 (MQ=255)

                                                               cTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGac                           >  1:4216427/1‑93 (MQ=255)

                                                                 cATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGacac                         <  1:3308310/93‑1 (MQ=255)

                                                                  aTGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAcc                        >  1:3776853/1‑93 (MQ=255)

                                                                   tGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAccc                       >  2:2200889/1‑93 (MQ=255)

                                                                    gCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCg                      >  1:6522561/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  2:1266047/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  2:6477660/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  1:1973957/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTAT‑A‑CACC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  2:7586049/1‑93 (MQ=255)

                                                                       aTAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGttt                   >  1:6422548/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  <  1:1659724/93‑1 (MQ=255)

                                                                        tAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  >  2:1655454/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  <  2:5660707/93‑1 (MQ=255)

                                                                            cGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCg              <  1:4420668/93‑1 (MQ=255)

                                                                            cGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCg              <  1:5642403/93‑1 (MQ=255)

                                                                            cGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCg              <  1:7680506/93‑1 (MQ=255)

                                                                             gCTGGACTTAACTAT‑A‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTc            >  2:2007584/1‑93 (MQ=255)

                                                                              cTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTc            >  1:6726908/1‑93 (MQ=255)

                                                                               tGGACTTAACTATGC‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCg           <  2:6709646/93‑1 (MQ=255)

                                                                                ggACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGa          >  1:5103280/1‑93 (MQ=255)

                                                                                  aCTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtt        <  1:3677365/93‑1 (MQ=255)

                                                                                   cTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAttt       >  1:7283441/1‑93 (MQ=255)

                                                                                   cTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAttt       >  1:1356631/1‑93 (MQ=255)

                                                                                    ttAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtttt      >  1:2276160/1‑93 (MQ=255)

                                                                                     tAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTa     >  1:4118182/1‑93 (MQ=255)

                                                                                       aCTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTacc   >  2:6821286/1‑93 (MQ=255)

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT  >  NC_000913/2043936‑2044115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  NC_000913/2043940‑2044126

TGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCC                                                                                               >  SRR4302543.5664631/1‑94 (MQ=60)

  AACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCCT                                                                                             >  SRR4302543.6072814/1‑94 (MQ=60)

  AACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCCT                                                                                             >  SRR4302543.5247825/1‑94 (MQ=60)

     AGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCC                                                                                           >  SRR4302543.7046355/1‑94 (MQ=60)

     AGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCC                                                                                           <  SRR4302543.1072383/94‑1 (MQ=60)

      GTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCC                                                                                          <  SRR4302543.1445379/94‑1 (MQ=60)

          TATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTG                                                                                      <  SRR4302543.7865292/94‑1 (MQ=60)

          TATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTG                                                                                      <  SRR4302543.7038101/94‑1 (MQ=60)

           ATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGA                                                                                     >  SRR4302543.2235505/1‑94 (MQ=60)

            TGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGAT                                                                                    >  SRR4302543.7264242/1‑94 (MQ=60)

             GGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATG                                                                                   <  SRR4302543.748636/94‑1 (MQ=60)

               CGCTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGAC                                                                                 <  SRR4302543.4447019/94‑1 (MQ=60)

               CGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGAC                                                                                 >  SRR4302543.4259151/1‑94 (MQ=60)

               CGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGAC                                                                                 >  SRR4302543.6561896/1‑94 (MQ=60)

               CGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGAC                                                                                 <  SRR4302543.4447020/94‑1 (MQ=60)

                     AGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAG                                                                           <  SRR4302543.5468312/94‑1 (MQ=60)

                        GGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGC                                                                        >  SRR4302543.3780229/1‑94 (MQ=60)

                        GGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGC                                                                        <  SRR4302543.39560/94‑1 (MQ=60)

                         GCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCG                                                                       <  SRR4302543.4614693/94‑1 (MQ=60)

                          CCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGG                                                                      >  SRR4302543.189429/1‑94 (MQ=60)

                          CCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGG                                                                      >  SRR4302543.2963385/1‑94 (MQ=60)

                           CCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGA                                                                     <  SRR4302543.7405108/94‑1 (MQ=60)

                           CCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGA                                                                     >  SRR4302543.6669467/1‑94 (MQ=60)

                           CCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGA                                                                     >  SRR4302543.5889811/1‑94 (MQ=60)

                            CTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAG                                                                    >  SRR4302543.1636171/1‑94 (MQ=60)

                            CTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAG                                                                    >  SRR4302543.7763120/1‑94 (MQ=60)

                            CTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAG                                                                    >  SRR4302543.6438937/1‑94 (MQ=60)

                            CTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAG                                                                    >  SRR4302543.6375781/1‑94 (MQ=60)

                                TCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAAC                                                                >  SRR4302543.6653927/1‑94 (MQ=60)

                                 CGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACG                                                               >  SRR4302543.4323342/1‑94 (MQ=60)

                                 CGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACG                                                               >  SRR4302543.7538717/1‑94 (MQ=60)

                                  GATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGC                                                              >  SRR4302543.2988953/1‑94 (MQ=60)

                                  GATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGC                                                              >  SRR4302543.970488/1‑94 (MQ=60)

                                  GATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGC                                                              >  SRR4302543.3432242/1‑94 (MQ=60)

                                   ATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCC                                                             >  SRR4302543.1648249/1‑94 (MQ=60)

                                   ATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCA                                                            >  SRR4302543.7700766/1‑94 (MQ=60)

                                    TAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCCACCCCA                                                            >  SRR4302543.2196522/1‑94 (MQ=60)

                                     AAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAG                                                           <  SRR4302543.84750/94‑1 (MQ=60)

                                      aaaaccgTCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGG                                                          <  SRR4302543.1599154/87‑1 (MQ=60)

                                      AAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGG                                                          <  SRR4302543.6563844/94‑1 (MQ=60)

                                       AGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGG                                                         >  SRR4302543.6101264/1‑94 (MQ=60)

                                        GACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGA                                                        >  SRR4302543.6137672/1‑94 (MQ=60)

                                         ACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTA                                                       >  SRR4302543.753225/1‑94 (MQ=60)

                                         ACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTA                                                       <  SRR4302543.2809840/94‑1 (MQ=60)

                                          CGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAA                                                      <  SRR4302543.5437079/94‑1 (MQ=60)

                                             TCGGCAAGGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑TCC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACA                                                   <  SRR4302543.3197127/94‑1 (MQ=60)

                                             TCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACA                                                   <  SRR4302543.6279923/94‑1 (MQ=60)

                                             TCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACA                                                   <  SRR4302543.4100921/94‑1 (MQ=60)

                                             TCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCACGGTAAACA                                                   >  SRR4302543.4046059/1‑94 (MQ=60)

                                               GGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGG                                                 >  SRR4302543.1512434/1‑94 (MQ=60)

                                               GGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTGCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGG                                                 <  SRR4302543.7681147/94‑1 (MQ=60)

                                                 CAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGC                                               >  SRR4302543.5289541/1‑94 (MQ=60)

                                                 CAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGC                                               >  SRR4302543.5388077/1‑94 (MQ=60)

                                                 CAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGC                                               >  SRR4302543.3743532/1‑94 (MQ=60)

                                                    AGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAA                                            >  SRR4302543.4465399/1‑94 (MQ=60)

                                                    AGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAA                                            <  SRR4302543.5219224/94‑1 (MQ=60)

                                                     GTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAA                                           >  SRR4302543.2518779/1‑94 (MQ=60)

                                                     GTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAA                                           >  SRR4302543.7591409/1‑94 (MQ=60)

                                                      TAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAA                                          <  SRR4302543.1121421/94‑1 (MQ=60)

                                                      TAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAA                                          >  SRR4302543.7548610/1‑94 (MQ=60)

                                                      TAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAA                                          <  SRR4302543.5271355/94‑1 (MQ=60)

                                                       AATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAT                                         <  SRR4302543.2118997/94‑1 (MQ=60)

                                                       AATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAT                                         <  SRR4302543.5549081/94‑1 (MQ=60)

                                                       AATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAT                                         <  SRR4302543.7494027/94‑1 (MQ=60)

                                                        ATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATG                                        >  SRR4302543.7429453/1‑94 (MQ=60)

                                                        ATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATG                                        >  SRR4302543.2902762/1‑94 (MQ=60)

                                                        ATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATG                                        <  SRR4302543.2348377/94‑1 (MQ=60)

                                                        ATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATG                                        <  SRR4302543.3694648/94‑1 (MQ=60)

                                                          CCTCATGCCATAACCGCTGGACTTAACTA‑TCC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGAC                                      <  SRR4302543.7123338/94‑1 (MQ=60)

                                                          CCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGAC                                      >  SRR4302543.4254655/1‑94 (MQ=60)

                                                           CTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACA                                     >  SRR4302543.1996106/1‑94 (MQ=60)

                                                           CTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACA                                     >  SRR4302543.7620125/1‑94 (MQ=60)

                                                            TCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAC                                    >  SRR4302543.6944625/1‑94 (MQ=60)

                                                             CATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACC                                   >  SRR4302543.3810107/1‑94 (MQ=60)

                                                             CATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACC                                   <  SRR4302543.6981060/94‑1 (MQ=60)

                                                             CATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACC                                   <  SRR4302543.3338168/94‑1 (MQ=60)

                                                              ATGCCATAACCGCTTGACTTAACTATT‑C‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGAGACCC                                  <  SRR4302543.1730283/94‑1 (MQ=60)

                                                              ATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCC                                  >  SRR4302543.162474/1‑94 (MQ=60)

                                                               TGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCG                                 >  SRR4302543.6583983/1‑94 (MQ=60)

                                                                 CCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTT                               >  SRR4302543.1991654/1‑94 (MQ=60)

                                                                 CCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGCT                               >  SRR4302543.3529991/1‑94 (MQ=60)

                                                                  CATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTT                              >  SRR4302543.4183834/1‑94 (MQ=60)

                                                                  CATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTT                              >  SRR4302543.6483068/1‑94 (MQ=60)

                                                                  CATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTT                              >  SRR4302543.1112033/1‑94 (MQ=60)

                                                                   ATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTT                             <  SRR4302543.7367789/94‑1 (MQ=60)

                                                                   ATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTT                             >  SRR4302543.3377246/1‑94 (MQ=60)

                                                                   ATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTT                             >  SRR4302543.5882378/1‑94 (MQ=60)

                                                                   ATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTT                             >  SRR4302543.6720023/1‑94 (MQ=60)

                                                                    TAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTG                            <  SRR4302543.1674587/94‑1 (MQ=60)

                                                                     AACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGC                           >  SRR4302543.6524536/1‑94 (MQ=60)

                                                                     AACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGC                           >  SRR4302543.4633510/1‑94 (MQ=60)

                                                                        CGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGT                        <  SRR4302543.4460734/94‑1 (MQ=60)

                                                                        CGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGT                        <  SRR4302543.7753258/94‑1 (MQ=60)

                                                                        CGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGT                        <  SRR4302543.5693842/94‑1 (MQ=60)

                                                                         GCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTC                       >  SRR4302543.6790464/1‑94 (MQ=60)

                                                                         GCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTC                       <  SRR4302543.3928953/94‑1 (MQ=60)

                                                                           TGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGA                     >  SRR4302543.5149473/1‑94 (MQ=60)

                                                                           TGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGA                     >  SRR4302543.7548163/1‑94 (MQ=60)

                                                                            GGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAT                    <  SRR4302543.1910968/94‑1 (MQ=60)

                                                                              ACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTT                  >  SRR4302543.1368768/1‑94 (MQ=60)

                                                                              ACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTT                  <  SRR4302543.3709660/94‑1 (MQ=60)

                                                                              ACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTT                  >  SRR4302543.7353771/1‑94 (MQ=60)

                                                                               CTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTT                 >  SRR4302543.2296982/1‑94 (MQ=60)

                                                                               CTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTT                 >  SRR4302543.3729872/1‑94 (MQ=60)

                                                                                TTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTA                >  SRR4302543.4155287/1‑94 (MQ=60)

                                                                                  AACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACC              <  SRR4302543.2225158/94‑1 (MQ=60)

                                                                                  AACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACC              <  SRR4302543.6022512/94‑1 (MQ=60)

                                                                                    aactgtcgccgCCTTCCCGCTGAGGACCCCCAGCGCGGAGCAACGCCCGGGTACACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACC              <  SRR4302543.2225159/83‑1 (MQ=60)

                                                                                    aactattcCCCCCTGCCCGCTGATGACCTTCCGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACC              <  SRR4302543.5561948/86‑1 (MQ=60)

                                                                                    CTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTG            >  SRR4302543.6542527/1‑94 (MQ=60)

                                                                                     TA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGG           <  SRR4302543.6262230/94‑1 (MQ=60)

                                                                                     TA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGG           <  SRR4302543.874690/94‑1 (MQ=60)

                                                                                     TA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGG           <  SRR4302543.494564/94‑1 (MQ=60)

                                                                                         tacCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCA         >  SRR4302543.1205593/4‑94 (MQ=60)

                                                                                          C‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAAC       <  SRR4302543.3461616/94‑1 (MQ=60)

                                                                                            CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGCTTTTACCTGCCAACT      >  SRR4302543.1846150/1‑94 (MQ=60)

                                                                                            CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACC      >  SRR4302543.2672279/1‑94 (MQ=60)

                                                                                            CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACC      >  SRR4302543.6895150/1‑94 (MQ=60)

                                                                                            CCCCCTTCCCGCTGATGACCCTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTCTGCCGTCGATGTTACCTGGCAACC      >  SRR4302543.7075303/1‑94 (MQ=60)

                                                                                             CCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCT     <  SRR4302543.5179048/94‑1 (MQ=60)

                                                                                              CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTG    <  SRR4302543.7890615/94‑1 (MQ=60)

                                                                                              CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTG    >  SRR4302543.2190992/1‑94 (MQ=60)

                                                                                              CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTG    >  SRR4302543.7235409/1‑94 (MQ=60)

                                                                                               CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGG   >  SRR4302543.7831530/1‑94 (MQ=60)

                                                                                               CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGG   <  SRR4302543.6865288/94‑1 (MQ=60)

                                                                                               CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGG   >  SRR4302543.6200912/1‑94 (MQ=60)

                                                                                               CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGG   <  SRR4302543.5449408/94‑1 (MQ=60)

                                                                                                CTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  SRR4302543.771589/1‑94 (MQ=60)

                                                                                                CTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  SRR4302543.7530625/1‑94 (MQ=60)

                                                                                                CTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTAGCAACCTGGC  >  SRR4302543.771590/1‑94 (MQ=60)

                                                                                                CTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAACGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  SRR4302543.7129565/1‑94 (MQ=60)

TGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  NC_000913/2043940‑2044126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`