Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,045,049 T→C V722A (GTC→GCC)  b1978 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,045,0490TC100.0% 756.4 / NA 238V722A (GTC→GCC) b1978hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (119/119);  total (119/119)

Only 100 of 250 total aligned reads displayed.

AGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGTCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCG  >  NC_000913/2044959‑2045138
                                                                                          |                                                                                         
    aCAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATaa                                                                                     >  1:4549270/1‑93 (MQ=255)
    aCAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATaa                                                                                     <  2:1499863/93‑1 (MQ=255)
    aCAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATaa                                                                                     >  1:4333799/1‑93 (MQ=255)
    aCAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATaa                                                                                     >  2:6469836/1‑93 (MQ=255)
      aaaaaCAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAgg                                                                                   <  2:436814/93‑1 (MQ=255)
        aaaCAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCg                                                                                 >  1:6669401/1‑93 (MQ=255)
         aaCAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATCAGGCGa                                                                                >  2:1075355/1‑93 (MQ=255)
         aaCAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGa                                                                                >  2:6955047/1‑93 (MQ=255)
           cAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGAcc                                                                              <  1:3807106/93‑1 (MQ=255)
           cAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGAcc                                                                              <  2:545400/93‑1 (MQ=255)
            aGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCt                                                                             <  1:1948633/93‑1 (MQ=255)
             gCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCta                                                                            >  1:7138670/1‑93 (MQ=255)
               aaCTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCtata                                                                          >  1:5124264/1‑93 (MQ=255)
               aaCTGAATAACGCAGTCAGCATCGAACACGTGAAACCAGGAGTCCCTACAGACTGGCAAGAAACAGCAGCTGGGGCCGATACGCCGACAtata                                                                          >  1:5961633/1‑93 (MQ=255)
                aCTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATAc                                                                         >  2:2780022/1‑93 (MQ=255)
                 cTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATAcc                                                                        <  1:3882593/93‑1 (MQ=255)
                 cTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATAcc                                                                        >  1:4449569/1‑93 (MQ=255)
                  tGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCg                                                                       >  1:2938046/1‑93 (MQ=255)
                  tGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCg                                                                       >  2:653287/1‑93 (MQ=255)
                  tGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCg                                                                       >  2:3531619/1‑93 (MQ=255)
                   gAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGc                                                                      >  2:4264622/1‑93 (MQ=255)
                      tAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCta                                                                   <  2:742747/93‑1 (MQ=255)
                        aCGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCtata                                                                 >  1:7389399/1‑93 (MQ=255)
                        aCGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCtata                                                                 >  1:7783911/1‑93 (MQ=255)
                          gCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATAcc                                                               >  1:1358965/1‑93 (MQ=255)
                              tCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAg                                                           <  2:6721111/93‑1 (MQ=255)
                              tCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAg                                                           >  2:4985208/1‑93 (MQ=255)
                                aGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGc                                                         >  1:7216013/1‑93 (MQ=255)
                                 gCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCa                                                        >  1:5762201/1‑93 (MQ=255)
                                  cATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAg                                                       <  1:4411210/93‑1 (MQ=255)
                                  cATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAg                                                       <  2:1386645/93‑1 (MQ=255)
                                   aTCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGt                                                      >  2:644253/1‑93 (MQ=255)
                                   aTCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGt                                                      >  1:7546334/1‑93 (MQ=255)
                                   aTCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGt                                                      >  1:3225758/1‑93 (MQ=255)
                                    tCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTg                                                     <  1:595617/93‑1 (MQ=255)
                                     cGACAACGTGAAACCAGGAGTCACTACAGACTGAAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCCAAGGCAGTgg                                                    <  1:7016324/93‑1 (MQ=255)
                                      gACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGa                                                   >  1:4107561/1‑93 (MQ=255)
                                       aCAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGAc                                                  >  2:3017840/1‑93 (MQ=255)
                                        cAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACt                                                 <  2:4709591/93‑1 (MQ=255)
                                         aaCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGCTGGCGCCTATAAGGCGACCTATACCGCCTATACCAACGGCAGTGGACtt                                                >  1:6557212/1‑93 (MQ=255)
                                         aaCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACtt                                                <  1:2276011/93‑1 (MQ=255)
                                         aaCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACtt                                                <  1:4207949/93‑1 (MQ=255)
                                          aCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACTGCCTATATCAAAGGAAGTGGACTTa                                               >  2:4076562/1‑93 (MQ=255)
                                          aCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTa                                               >  1:331251/1‑93 (MQ=255)
                                          aCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTa                                               <  2:736956/93‑1 (MQ=255)
                                          aCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTa                                               <  2:5939310/93‑1 (MQ=255)
                                          aCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTa                                               >  2:5884459/1‑93 (MQ=255)
                                            gTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACt                                             <  2:1417865/93‑1 (MQ=255)
                                            gTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACt                                             <  2:3843775/93‑1 (MQ=255)
                                             tGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTg                                            >  2:4864004/1‑93 (MQ=255)
                                             tGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTg                                            <  2:2235882/93‑1 (MQ=255)
                                             tGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTg                                            >  2:7695512/1‑93 (MQ=255)
                                              gAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGc                                           <  1:540594/93‑1 (MQ=255)
                                              gAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGc                                           <  1:6203375/93‑1 (MQ=255)
                                                 aCCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGaa                                        >  1:4668820/1‑93 (MQ=255)
                                                   cAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGc                                      <  2:7157363/93‑1 (MQ=255)
                                                   cAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGc                                      >  1:6638333/1‑93 (MQ=255)
                                                     ggAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTa                                    >  1:5431585/1‑93 (MQ=255)
                                                     ggAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTa                                    <  1:5861647/93‑1 (MQ=255)
                                                     ggAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTa                                    >  1:3189185/1‑93 (MQ=255)
                                                      gAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAt                                   <  2:6126472/93‑1 (MQ=255)
                                                      gAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAt                                   <  1:6394442/93‑1 (MQ=255)
                                                       aGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAtt                                  <  2:1608192/93‑1 (MQ=255)
                                                        gTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTa                                 >  2:5958488/1‑93 (MQ=255)
                                                         tCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTaa                                <  1:2882226/93‑1 (MQ=255)
                                                          cACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAAt                               >  2:3786688/1‑93 (MQ=255)
                                                           aCTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATg                              >  1:2481425/1‑93 (MQ=255)
                                                            cTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGc                             <  2:11469/93‑1 (MQ=255)
                                                            cTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGc                             >  1:3971800/1‑93 (MQ=255)
                                                             tACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCa                            >  1:2625462/1‑93 (MQ=255)
                                                              aCAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCaa                           <  2:3400411/93‑1 (MQ=255)
                                                               cAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCaaa                          <  1:7918384/93‑1 (MQ=255)
                                                                aGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCaaaa                         >  1:1568472/1‑93 (MQ=255)
                                                                 gACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAc                        <  2:5808419/93‑1 (MQ=255)
                                                                 gACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAc                        >  1:1903346/1‑93 (MQ=255)
                                                                 gACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAc                        >  1:1809002/1‑93 (MQ=255)
                                                                 gACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAc                        >  1:1681001/1‑93 (MQ=255)
                                                                  aCTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACt                       <  1:4505337/93‑1 (MQ=255)
                                                                    tGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTgg                     >  2:6384256/1‑93 (MQ=255)
                                                                    tGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTgg                     >  1:5386758/1‑93 (MQ=255)
                                                                    tGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTgg                     >  1:2421849/1‑93 (MQ=255)
                                                                    tGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTgg                     >  1:1101716/1‑93 (MQ=255)
                                                                     ggAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGa                    <  2:2454629/93‑1 (MQ=255)
                                                                      gaaagaacCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGaa                   <  1:4750420/93‑1 (MQ=255)
                                                                      gaaagaaaCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCCAAGGCAGTGGACTTACTGCGAGGCTATTAATCAACAACTGGaa                   >  1:5899945/1‑93 (MQ=255)
                                                                      gaaagaaaCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGaa                   >  1:1295523/1‑93 (MQ=255)
                                                                       aaagaaaCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAAt                  <  2:4654633/93‑1 (MQ=255)
                                                                       aaagaaaCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAAt                  >  2:7665455/1‑93 (MQ=255)
                                                                        aagaaaCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATg                 <  1:6779203/93‑1 (MQ=255)
                                                                         agaaaCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGa                <  2:4041904/93‑1 (MQ=255)
                                                                          gaaaCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGaa               >  2:7209910/1‑93 (MQ=255)
                                                                           aaaCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAg              <  2:558131/93‑1 (MQ=255)
                                                                              ccGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGAtt           <  1:3510996/93‑1 (MQ=255)
                                                                               cGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGAttt          <  1:4061073/93‑1 (MQ=255)
                                                                               cGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGAttt          >  1:1497939/1‑93 (MQ=255)
                                                                               cGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGAttt          <  2:822527/93‑1 (MQ=255)
                                                                                   gATGGCGCCTATAAGGCGTCCTATACCGCATATGCCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCAt      <  2:7232482/93‑1 (MQ=255)
                                                                                   gATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCAt      >  2:852065/1‑93 (MQ=255)
                                                                                    aTGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATa     >  1:1404237/1‑93 (MQ=255)
                                                                                     tGGCGCCTATAAGGCTACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATAc    <  1:6252/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGTCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCG  >  NC_000913/2044959‑2045138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGTCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGG  >  NC_000913/2044958‑2045142
                                                                                           |                                                                                             
AAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGCCAACGTGAAACCAGGAGTCCATACAGACTGGAAACAAACAGCAGATGGCGCCT                                                                                             >  SRR4302543.3245167/1‑94 (MQ=60)
AAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCT                                                                                             <  SRR4302543.6486312/94‑1 (MQ=60)
 AGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTA                                                                                            >  SRR4302543.5477876/1‑94 (MQ=60)
    AACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAA                                                                                         >  SRR4302543.4590702/1‑94 (MQ=60)
    AACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAA                                                                                         >  SRR4302543.4373116/1‑94 (MQ=60)
    AACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAA                                                                                         >  SRR4302543.4373115/1‑94 (MQ=60)
      CAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGG                                                                                       <  SRR4302543.1357575/94‑1 (MQ=60)
       AAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGC                                                                                      >  SRR4302543.1208816/1‑94 (MQ=60)
       AAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGC                                                                                      <  SRR4302543.988446/94‑1 (MQ=60)
        AAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCG                                                                                     >  SRR4302543.453063/1‑94 (MQ=60)
        AAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCG                                                                                     >  SRR4302543.6732372/1‑94 (MQ=60)
          AACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGAC                                                                                   <  SRR4302543.5719240/94‑1 (MQ=60)
           ACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACC                                                                                  <  SRR4302543.4698622/94‑1 (MQ=60)
            CAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCT                                                                                 <  SRR4302543.3840671/94‑1 (MQ=60)
             AGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTA                                                                                >  SRR4302543.7208035/1‑94 (MQ=60)
             AGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTA                                                                                <  SRR4302543.1966115/94‑1 (MQ=60)
              GCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTAT                                                                               <  SRR4302543.3275278/94‑1 (MQ=60)
              GCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTAT                                                                               <  SRR4302543.7400545/94‑1 (MQ=60)
               CAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATA                                                                              >  SRR4302543.5170593/1‑94 (MQ=60)
               CAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATA                                                                              >  SRR4302543.6016217/1‑94 (MQ=60)
                 ACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACC                                                                            <  SRR4302543.3947550/94‑1 (MQ=60)
                 ACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACC                                                                            >  SRR4302543.4489871/1‑94 (MQ=60)
                 ACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACC                                                                            <  SRR4302543.247213/94‑1 (MQ=60)
                 ACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACC                                                                            <  SRR4302543.6243035/94‑1 (MQ=60)
                 ACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACC                                                                            <  SRR4302543.6713333/94‑1 (MQ=60)
                 ACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACC                                                                            <  SRR4302543.7504952/94‑1 (MQ=60)
                  CTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCG                                                                           <  SRR4302543.2443219/94‑1 (MQ=60)
                  CTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCG                                                                           >  SRR4302543.1591016/1‑94 (MQ=60)
                  CTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCG                                                                           >  SRR4302543.2964732/1‑94 (MQ=60)
                  CTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCG                                                                           <  SRR4302543.3916872/94‑1 (MQ=60)
                     AATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCT                                                                        >  SRR4302543.3303814/1‑94 (MQ=60)
                       TAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTAT                                                                      <  SRR4302543.3581951/94‑1 (MQ=60)
                        AACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATA                                                                     >  SRR4302543.7460386/1‑94 (MQ=60)
                        AACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATA                                                                     >  SRR4302543.7857295/1‑94 (MQ=60)
                          CGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACC                                                                   >  SRR4302543.1371132/1‑94 (MQ=60)
                          CGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGACGGCGCCTATAAGGCGACCTATACCGCCTATACA                                                                   >  SRR4302543.1371134/1‑94 (MQ=60)
                           GCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCA                                                                  <  SRR4302543.6865097/94‑1 (MQ=60)
                           GCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCA                                                                  <  SRR4302543.6117469/94‑1 (MQ=60)
                             AGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAA                                                                <  SRR4302543.6287281/94‑1 (MQ=60)
                              GTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAG                                                               <  SRR4302543.1463083/94‑1 (MQ=60)
                              GTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAG                                                               >  SRR4302543.4459922/1‑94 (MQ=60)
                               TCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGG                                                              >  SRR4302543.1521079/1‑94 (MQ=60)
                                CAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGC                                                             >  SRR4302543.7285957/1‑94 (MQ=60)
                                 AGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCA                                                            >  SRR4302543.6739727/1‑94 (MQ=60)
                                 AGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCA                                                            >  SRR4302543.5814691/1‑94 (MQ=60)
                                  acaccgacgACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAG                                                           <  SRR4302543.2078274/85‑1 (MQ=60)
                                  GCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAG                                                           <  SRR4302543.2869686/94‑1 (MQ=60)
                                  GCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAG                                                           <  SRR4302543.5837925/94‑1 (MQ=60)
                                   TATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGT                                                          >  SRR4302543.3254920/1‑94 (MQ=60)
                                   CATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGT                                                          <  SRR4302543.4451193/94‑1 (MQ=60)
                                   CATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGT                                                          >  SRR4302543.7618229/1‑94 (MQ=60)
                                   CATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGT                                                          >  SRR4302543.1583621/1‑94 (MQ=60)
                                    ATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTG                                                         >  SRR4302543.2680147/1‑94 (MQ=60)
                                    ATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTG                                                         <  SRR4302543.4306042/94‑1 (MQ=60)
                                     TCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGG                                                        <  SRR4302543.601081/94‑1 (MQ=60)
                                      CGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGA                                                       >  SRR4302543.177201/1‑94 (MQ=60)
                                      CGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGA                                                       <  SRR4302543.4044293/94‑1 (MQ=60)
                                      CGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGA                                                       >  SRR4302543.4144513/1‑94 (MQ=60)
                                      CGACAACGTGAAACCAGGAGTCACTACAGACTGAAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCCAAGGCAGTGGA                                                       <  SRR4302543.7083365/94‑1 (MQ=60)
                                        ACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACT                                                     >  SRR4302543.4583650/1‑94 (MQ=60)
                                         CAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGCTGGCGCCTATAAGGCGACCTATACCGCCTATACCAACGGCAGTGGACTT                                                    >  SRR4302543.6618912/1‑94 (MQ=60)
                                         CAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCTCCTATAAGGAGACCTATACCGCCTATACCAAAGGCAGTGGACTT                                                    >  SRR4302543.7739276/1‑94 (MQ=60)
                                         CAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTT                                                    <  SRR4302543.7189062/94‑1 (MQ=60)
                                          AACGTGAAACCGGGAGTCACTACAAACTGTAAAGAAACCGCAGATGGCTCCTATAAGGCAACCTATACCGCCTATACCAAAGGCAGTGGACTTA                                                   <  SRR4302543.2296831/94‑1 (MQ=60)
                                          AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTA                                                   >  SRR4302543.334286/1‑94 (MQ=60)
                                          AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTA                                                   <  SRR4302543.4246102/94‑1 (MQ=60)
                                          AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTA                                                   <  SRR4302543.2296830/94‑1 (MQ=60)
                                             GTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTG                                                >  SRR4302543.631929/1‑94 (MQ=60)
                                              TGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGC                                               >  SRR4302543.246392/1‑94 (MQ=60)
                                               GAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCG                                              <  SRR4302543.545594/94‑1 (MQ=60)
                                               GAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCG                                              <  SRR4302543.6261955/94‑1 (MQ=60)
                                               GAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCG                                              >  SRR4302543.7424419/1‑94 (MQ=60)
                                               GAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCG                                              <  SRR4302543.1862431/94‑1 (MQ=60)
                                                 AACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAA                                            >  SRR4302543.4711373/1‑94 (MQ=60)
                                                 AACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAA                                            >  SRR4302543.4711374/1‑94 (MQ=60)
                                                   CCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGC                                          >  SRR4302543.5286160/1‑94 (MQ=60)
                                                   CCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGC                                          >  SRR4302543.3399344/1‑94 (MQ=60)
                                                   CCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGC                                          >  SRR4302543.6700978/1‑94 (MQ=60)
                                                   CCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAAACAGAGGAACTACTGCGAAGC                                          >  SRR4302543.6700977/1‑94 (MQ=60)
                                                    CAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCT                                         <  SRR4302543.2963563/94‑1 (MQ=60)
                                                     AGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTA                                        >  SRR4302543.5480935/1‑94 (MQ=60)
                                                     AGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTA                                        >  SRR4302543.3217944/1‑94 (MQ=60)
                                                     AGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTA                                        >  SRR4302543.5480936/1‑94 (MQ=60)
                                                      GGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAT                                       <  SRR4302543.4238033/94‑1 (MQ=60)
                                                      GGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAT                                       >  SRR4302543.6679171/1‑94 (MQ=60)
                                                      GGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAT                                       <  SRR4302543.5914992/94‑1 (MQ=60)
                                                      GGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAT                                       <  SRR4302543.5958667/94‑1 (MQ=60)
                                                      GGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACCTACTGCGAAGCTAT                                       >  SRR4302543.7090189/1‑94 (MQ=60)
                                                       GAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATT                                      <  SRR4302543.6454635/94‑1 (MQ=60)
                                                       GAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATT                                      <  SRR4302543.6454636/94‑1 (MQ=60)
                                                       GAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATT                                      <  SRR4302543.3014003/94‑1 (MQ=60)
                                                        AGTCACTACAGACTGGAAAGAAACCGCAGATGGTGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTA                                     <  SRR4302543.7717736/94‑1 (MQ=60)
                                                          TCACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAAT                                   <  SRR4302543.2908458/94‑1 (MQ=60)
                                                           CACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATG                                  <  SRR4302543.2541063/94‑1 (MQ=60)
                                                           CACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATG                                  >  SRR4302543.2219776/1‑94 (MQ=60)
                                                           CACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATG                                  >  SRR4302543.2503885/1‑94 (MQ=60)
                                                            ACTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGC                                 >  SRR4302543.4007009/1‑94 (MQ=60)
                                                             CTACAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCA                                >  SRR4302543.2649226/1‑94 (MQ=60)
                                                                CAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAA                             <  SRR4302543.7992763/94‑1 (MQ=60)
                                                                CAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAA                             >  SRR4302543.1582480/1‑94 (MQ=60)
                                                                CAGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAA                             <  SRR4302543.7992764/94‑1 (MQ=60)
                                                                 AGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAC                            <  SRR4302543.5175582/94‑1 (MQ=60)
                                                                 AGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAC                            >  SRR4302543.1920476/1‑94 (MQ=60)
                                                                 AGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAC                            >  SRR4302543.5998953/1‑94 (MQ=60)
                                                                 AGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAC                            >  SRR4302543.1825204/1‑94 (MQ=60)
                                                                 AGACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAAC                            >  SRR4302543.1696085/1‑94 (MQ=60)
                                                                  GACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACT                           <  SRR4302543.1318487/94‑1 (MQ=60)
                                                                   ACTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTG                          <  SRR4302543.4546299/94‑1 (MQ=60)
                                                                    CTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGG                         >  SRR4302543.1111641/1‑94 (MQ=60)
                                                                    CTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGG                         >  SRR4302543.2443713/1‑94 (MQ=60)
                                                                    CTGGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGG                         >  SRR4302543.5435688/1‑94 (MQ=60)
                                                                      GGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCCAAGGCAGTGGACTTACTGCGAGGCTATTAATCAACAACTGGAA                       >  SRR4302543.5953764/1‑94 (MQ=60)
                                                                      GGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAA                       >  SRR4302543.1307050/1‑94 (MQ=60)
                                                                      GGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAA                       <  SRR4302543.6511474/94‑1 (MQ=60)
                                                                      GGAAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAA                       >  SRR4302543.5953529/1‑94 (MQ=60)
                                                                       GACAGAAACCCCAGATGGCGCCTATAAGGCGCCCTAGACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAAT                      <  SRR4302543.4793842/94‑1 (MQ=60)
                                                                       GAAAGAACCCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAAT                      <  SRR4302543.4793843/94‑1 (MQ=60)
                                                                        AAAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATG                     >  SRR4302543.3811732/1‑94 (MQ=60)
                                                                         AAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGA                    <  SRR4302543.6843226/94‑1 (MQ=60)
                                                                         AAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGA                    <  SRR4302543.7273767/94‑1 (MQ=60)
                                                                         AAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGA                    <  SRR4302543.1901844/94‑1 (MQ=60)
                                                                         AAGAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGA                    >  SRR4302543.2987647/1‑94 (MQ=60)
                                                                           GAAGCCGCAGATGGCGCCTATAAGGCGACGTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAG                  <  SRR4302543.3998914/94‑1 (MQ=60)
                                                                           GAAACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAG                  <  SRR4302543.193771/94‑1 (MQ=60)
                                                                             AACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGAT                <  SRR4302543.4810058/94‑1 (MQ=60)
                                                                             AACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGAT                <  SRR4302543.3841232/94‑1 (MQ=60)
                                                                              ACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATT               <  SRR4302543.4572988/94‑1 (MQ=60)
                                                                              ACCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATT               >  SRR4302543.5447100/1‑94 (MQ=60)
                                                                               CCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTT              >  SRR4302543.1511412/1‑94 (MQ=60)
                                                                               CCGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTT              <  SRR4302543.3542366/94‑1 (MQ=60)
                                                                                CGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTG             <  SRR4302543.4097441/94‑1 (MQ=60)
                                                                                CGCAGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTG             <  SRR4302543.4097440/94‑1 (MQ=60)
                                                                                CGCAGATGGCGCCTATAACGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTG             <  SRR4302543.4097442/94‑1 (MQ=60)
                                                                                   AGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCAT          >  SRR4302543.6775836/1‑94 (MQ=60)
                                                                                   AGATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCAT          >  SRR4302543.4450083/1‑94 (MQ=60)
                                                                                    GATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATA         >  SRR4302543.1416914/1‑94 (MQ=60)
                                                                                     ATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATAC        >  SRR4302543.5822506/1‑94 (MQ=60)
                                                                                     ATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATAC        <  SRR4302543.7659473/94‑1 (MQ=60)
                                                                                     ATGGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATAC        <  SRR4302543.4487594/94‑1 (MQ=60)
                                                                                      TGGCGCCTATAAGGCTACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACC       <  SRR4302543.6304/94‑1 (MQ=60)
                                                                                       GGCGCCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCG      >  SRR4302543.1501568/1‑94 (MQ=60)
                                                                                           CCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGG  >  SRR4302543.3875323/1‑94 (MQ=60)
                                                                                           CCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGG  <  SRR4302543.4318065/94‑1 (MQ=60)
                                                                                           CCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGG  >  SRR4302543.4457384/1‑94 (MQ=60)
                                                                                           |                                                                                             
AAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGACAACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGATGGCGTCTATAAGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGG  >  NC_000913/2044958‑2045142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑