Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,919	C→T	R156H (CGC→CAC)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,919	0	C	T	100.0%	855.4 / NA	242	R156H (CGC→CAC)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (112/130); total (112/130)

Only 100 of 248 total aligned reads displayed.

GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC  >  NC_000913/2259829‑2260008

ggTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGa                                                                                         <  1:2019513/93‑1 (MQ=255)

 gTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAg                                                                                        >  1:4330390/1‑93 (MQ=255)

      aCCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATc                                                                                   >  2:1565303/1‑93 (MQ=255)

      aCCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATc                                                                                   <  2:1920667/93‑1 (MQ=255)

       ccAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATcc                                                                                  <  1:2285097/93‑1 (MQ=255)

        cAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCa                                                                                 <  1:7722714/93‑1 (MQ=255)

          gCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGt                                                                               >  1:5866727/1‑93 (MQ=255)

           ccACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTc                                                                              <  2:5976772/93‑1 (MQ=255)

            cACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCg                                                                             >  2:139817/1‑93 (MQ=255)

             aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            <  2:2375145/93‑1 (MQ=255)

              cGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGt                                                                           >  1:4066948/1‑93 (MQ=255)

                 tGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGaa                                                                        <  1:5212947/93‑1 (MQ=255)

                     gCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCt                                                                    >  1:1003511/1‑93 (MQ=255)

                     gCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCt                                                                    >  2:5630063/1‑93 (MQ=255)

                      cTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCtt                                                                   >  2:3820633/1‑93 (MQ=255)

                       tttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTc                                                                  <  1:1103948/93‑1 (MQ=255)

                       tttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTc                                                                  <  2:6117923/93‑1 (MQ=255)

                       tttCAAACCTGCTACTAACGCTACACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTc                                                                  <  1:3872113/93‑1 (MQ=255)

                        ttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTcc                                                                 <  2:5096831/93‑1 (MQ=255)

                         tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCg                                                                <  2:247842/93‑1 (MQ=255)

                         tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCg                                                                <  2:7065535/93‑1 (MQ=255)

                          cAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCgg                                                               <  1:7313593/93‑1 (MQ=255)

                            aaCCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGc                                                             <  2:3741523/93‑1 (MQ=255)

                            aaCCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGc                                                             >  1:85646/1‑93 (MQ=255)

                             aCCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCt                                                            <  1:2976413/93‑1 (MQ=255)

                               cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  1:2060545/93‑1 (MQ=255)

                               cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  2:7722272/93‑1 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGACCGCTTCCGGGCTGAc                                                         >  1:408522/1‑93 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         <  2:1452776/93‑1 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         >  1:6786875/1‑93 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         >  1:1164610/1‑93 (MQ=255)

                                   tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                      >  2:7649328/1‑93 (MQ=255)

                                   tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                      >  1:2020917/1‑93 (MQ=255)

                                     ctaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTg                                                    <  1:6402652/93‑1 (MQ=255)

                                     ctaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTg                                                    <  1:7897284/93‑1 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   <  1:4166238/93‑1 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  1:1681349/1‑93 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  1:4664583/1‑93 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  1:3240662/1‑93 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGATGACGCCTGa                                                   >  2:4816859/1‑93 (MQ=255)

                                        aCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACg                                                 <  1:2426838/93‑1 (MQ=255)

                                        aCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTAACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACg                                                 <  2:541379/93‑1 (MQ=255)

                                         cggttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACgg                                                <  2:5963366/90‑1 (MQ=255)

                                            ttCACGGCTACTATCAAAGATAATTCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             <  2:2761496/93‑1 (MQ=255)

                                            ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             <  1:1778367/93‑1 (MQ=255)

                                            ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             <  2:7336706/93‑1 (MQ=255)

                                             tCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                                            <  2:7576012/93‑1 (MQ=255)

                                             tCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                                            <  1:3831422/93‑1 (MQ=255)

                                                cGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCtt                                         >  1:1523078/1‑93 (MQ=255)

                                                 ggCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCGTCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTc                                        <  2:1840781/93‑1 (MQ=255)

                                                 ggCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTc                                        <  2:1288205/93‑1 (MQ=255)

                                                    tactaTCAAAGATAATACAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGc                                     >  2:5221123/1‑93 (MQ=255)

                                                       taTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGa                                  >  2:648291/1‑93 (MQ=255)

                                                       taTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGa                                  <  1:1925946/93‑1 (MQ=255)

                                                       taTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGa                                  >  1:6345389/1‑93 (MQ=255)

                                                        aTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGac                                 <  2:3151265/93‑1 (MQ=255)

                                                          cAAAGATAATGCAAGGACCCTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGacac                               <  1:6565152/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              <  2:4363782/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  1:6086802/1‑93 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              <  2:1823878/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  2:3271827/1‑93 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  1:1425115/1‑93 (MQ=255)

                                                            aaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcacaa                             >  1:3182893/1‑92 (MQ=255)

                                                               aTAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAcc                          <  2:7894112/93‑1 (MQ=255)

                                                               aTAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAcc                          >  1:3447656/1‑93 (MQ=255)

                                                                 aaTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCat                        >  2:7219914/1‑93 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                       >  1:940178/1‑93 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                       >  2:5782589/1‑93 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGAGAGCTTCCGCTGACACAGACCata                       >  2:4308161/1‑93 (MQ=255)

                                                                   tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      >  2:6866202/1‑93 (MQ=255)

                                                                   tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      >  2:1030281/1‑93 (MQ=255)

                                                                   tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      <  2:801096/93‑1 (MQ=255)

                                                                    gCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATc                     >  2:6566987/1‑93 (MQ=255)

                                                                    gCAAGCCCACTGCCTCCGCAGGTGAGTCATCCAGTCGGTGATCGCTTCCGGCCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATc                     <  2:3413753/93‑1 (MQ=255)

                                                                       aGGACACTGACTCCGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  <  2:2090910/93‑1 (MQ=255)

                                                                       aGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  >  1:4962011/1‑93 (MQ=255)

                                                                       aGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  >  1:709538/1‑93 (MQ=255)

                                                                        ggACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAAc                 <  1:4774500/93‑1 (MQ=255)

                                                                         gACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACt                >  1:5709945/1‑93 (MQ=255)

                                                                         gACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACt                <  2:4714745/93‑1 (MQ=255)

                                                                          acacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTg               >  1:5264563/1‑93 (MQ=255)

                                                                          acacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTg               >  1:3801440/1‑93 (MQ=255)

                                                                            acTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAc             <  1:7468661/93‑1 (MQ=255)

                                                                             cTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAcc            >  1:3035719/1‑93 (MQ=255)

                                                                             cTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAcc            >  2:2666804/1‑93 (MQ=255)

                                                                             cTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAcc            >  1:7679108/1‑93 (MQ=255)

                                                                              tGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCg           >  1:741316/1‑93 (MQ=255)

                                                                              tGACTACGCAGGTCAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTACGCTGACACAGACCATATCGAACTGACCg           >  1:4540690/1‑93 (MQ=255)

                                                                               gccTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGa          <  2:2248987/91‑1 (MQ=255)

                                                                               gACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGa          >  1:2633622/1‑93 (MQ=255)

                                                                                 cTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGc        >  1:6447250/1‑93 (MQ=255)

                                                                                 cTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGc        >  1:1326958/1‑93 (MQ=255)

                                                                                 cTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGc        >  2:215250/1‑93 (MQ=255)

                                                                                  tACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGcc       <  1:3669783/93‑1 (MQ=255)

                                                                                   gcGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCa      <  2:3039490/92‑1 (MQ=255)

                                                                                   aCGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCa      <  2:6739348/93‑1 (MQ=255)

                                                                                    cGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAg     <  1:5464465/93‑1 (MQ=255)

                                                                                      cAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCt   >  1:533025/1‑93 (MQ=255)

                                                                                       aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  <  2:746691/93‑1 (MQ=255)

                                                                                       aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  >  2:1731652/1‑93 (MQ=255)

GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC  >  NC_000913/2259829‑2260008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAG  >  NC_000913/2259828‑2260012

AGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGA                                                                                             <  SRR4302543.1814605/94‑1 (MQ=60)

 GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAG                                                                                            >  SRR4302543.4369682/1‑94 (MQ=60)

 GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAG                                                                                            <  SRR4302543.2037656/94‑1 (MQ=60)

 AGTTTCACCAGCCACGGTGCCGGTTTCAAACCTGCTGCTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAG                                                                                            <  SRR4302543.2037657/94‑1 (MQ=60)

   TTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTC                                                                                          <  SRR4302543.7392962/94‑1 (MQ=60)

       ACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCC                                                                                      >  SRR4302543.11653/1‑94 (MQ=60)

        CCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCA                                                                                     <  SRR4302543.2305970/94‑1 (MQ=60)

         CAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAG                                                                                    <  SRR4302543.4550113/94‑1 (MQ=60)

         CAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAG                                                                                    <  SRR4302543.7795729/94‑1 (MQ=60)

          AGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGT                                                                                   <  SRR4302543.1179403/94‑1 (MQ=60)

          AGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGT                                                                                   >  SRR4302543.4762437/1‑94 (MQ=60)

          AGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGT                                                                                   >  SRR4302543.5920128/1‑94 (MQ=60)

          AGCCACGGTACCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGT                                                                                   <  SRR4302543.1659264/94‑1 (MQ=60)

           GCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTC                                                                                  <  SRR4302543.6203325/94‑1 (MQ=60)

           GCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTC                                                                                  <  SRR4302543.2000094/94‑1 (MQ=60)

            CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                                 <  SRR4302543.6475470/94‑1 (MQ=60)

            CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                                 <  SRR4302543.1061759/94‑1 (MQ=60)

            CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                                 <  SRR4302543.3901963/94‑1 (MQ=60)

            CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                                 <  SRR4302543.7781160/94‑1 (MQ=60)

              ACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGT                                                                               >  SRR4302543.4103374/1‑94 (MQ=60)

              ACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGT                                                                               <  SRR4302543.4669405/94‑1 (MQ=60)

                GGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGA                                                                             >  SRR4302543.3151281/1‑94 (MQ=60)

                GGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGA                                                                             >  SRR4302543.5426717/1‑94 (MQ=60)

                 GTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAA                                                                            <  SRR4302543.5018301/94‑1 (MQ=60)

                  TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                           >  SRR4302543.4349948/1‑94 (MQ=60)

                  TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                           <  SRR4302543.5259912/94‑1 (MQ=60)

                  TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                           <  SRR4302543.5763764/94‑1 (MQ=60)

                   TCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACG                                                                          <  SRR4302543.5345425/94‑1 (MQ=60)

                   GCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACG                                                                          <  SRR4302543.5345426/94‑1 (MQ=60)

                     CGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCT                                                                        >  SRR4302543.1012383/1‑94 (MQ=60)

                        TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCC                                                                     <  SRR4302543.1113902/94‑1 (MQ=60)

                        TTTCAAACCTGCTACTAACGCTACACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCC                                                                     <  SRR4302543.3906302/94‑1 (MQ=60)

                           CAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGG                                                                  <  SRR4302543.8932/94‑1 (MQ=60)

                           CAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGG                                                                  >  SRR4302543.7497171/1‑94 (MQ=60)

                           CAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGG                                                                  <  SRR4302543.7384076/94‑1 (MQ=60)

                            AAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGC                                                                 >  SRR4302543.86439/1‑94 (MQ=60)

                              ACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTG                                                               <  SRR4302543.3003444/94‑1 (MQ=60)

                              ACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGAAGGTGAGTCATCAAGTCGGTGAACGCTTCCGGGCTG                                                               <  SRR4302543.1390278/94‑1 (MQ=60)

                               CCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGA                                                              >  SRR4302543.4935174/1‑94 (MQ=60)

                                CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGACCGCTTCCGGGCTGAC                                                             >  SRR4302543.412292/1‑94 (MQ=60)

                                CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                             >  SRR4302543.6850973/1‑94 (MQ=60)

                                CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                             >  SRR4302543.412290/1‑94 (MQ=60)

                                CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                             <  SRR4302543.2078978/94‑1 (MQ=60)

                                CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                             >  SRR4302543.1175206/1‑94 (MQ=60)

                                CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATACAGTCGGAGAACGCTTCCGCGCTGAC                                                             >  SRR4302543.3953150/1‑94 (MQ=60)

                                  GCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGC                                                           <  SRR4302543.3108152/94‑1 (MQ=60)

                                   CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCC                                                          <  SRR4302543.7881829/94‑1 (MQ=60)

                                   CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCC                                                          >  SRR4302543.7633818/1‑94 (MQ=60)

                                   CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCC                                                          >  SRR4302543.2039074/1‑94 (MQ=60)

                                    TACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCT                                                         <  SRR4302543.6533170/94‑1 (MQ=60)

                                     ACTAACGCTTCACGTCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTG                                                        <  SRR4302543.53444/94‑1 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       >  SRR4302543.4707088/1‑94 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       >  SRR4302543.4707089/1‑94 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       >  SRR4302543.1696434/1‑94 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       >  SRR4302543.3827060/1‑94 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       >  SRR4302543.3269973/1‑94 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       <  SRR4302543.6462929/94‑1 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       <  SRR4302543.7971531/94‑1 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       >  SRR4302543.6692726/1‑94 (MQ=60)

                                      CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       >  SRR4302543.2599940/1‑94 (MQ=60)

                                       TAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAC                                                      <  SRR4302543.4203961/94‑1 (MQ=60)

                                       TAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAC                                                      <  SRR4302543.5291439/94‑1 (MQ=60)

                                        AACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACG                                                     <  SRR4302543.7558797/94‑1 (MQ=60)

                                         ACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGG                                                    <  SRR4302543.2448739/94‑1 (MQ=60)

                                         ACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCACTCGGTGAACGCTTCCGGGCTGACGCCTGACGG                                                    <  SRR4302543.1761834/94‑1 (MQ=60)

                                          CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGC                                                   >  SRR4302543.5679054/1‑94 (MQ=60)

                                          CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGA                                                   >  SRR4302543.7078010/1‑94 (MQ=60)

                                           GCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTA                                                  <  SRR4302543.3349693/94‑1 (MQ=60)

                                             TTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAG                                                <  SRR4302543.4149907/94‑1 (MQ=60)

                                             TTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAG                                                <  SRR4302543.1794255/94‑1 (MQ=60)

                                              TCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGC                                               <  SRR4302543.3882072/94‑1 (MQ=60)

                                              TCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGC                                               <  SRR4302543.3865218/94‑1 (MQ=60)

                                               CACGGCTACTATCAAAGATAATGCAATGACACTGGCTACGCAGGTGCGGGATCCGGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCT                                              >  SRR4302543.207581/1‑94 (MQ=60)

                                                ACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTT                                             >  SRR4302543.1536748/1‑94 (MQ=60)

                                                ACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTT                                             >  SRR4302543.1536749/1‑94 (MQ=60)

                                                      ACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTG                                       >  SRR4302543.3857817/1‑94 (MQ=60)

                                                       CTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGA                                      >  SRR4302543.6405056/1‑94 (MQ=60)

                                                        TATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAC                                     <  SRR4302543.6066387/94‑1 (MQ=60)

                                                        TATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAC                                     <  SRR4302543.1943296/94‑1 (MQ=60)

                                                         ATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACA                                    >  SRR4302543.3821853/1‑94 (MQ=60)

                                                          TCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACAC                                   >  SRR4302543.643778/1‑94 (MQ=60)

                                                           CAAAGATAATGCAAGGACCCTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                  <  SRR4302543.6626927/94‑1 (MQ=60)

                                                           CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                  <  SRR4302543.6626926/94‑1 (MQ=60)

                                                           CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                  >  SRR4302543.1438014/1‑94 (MQ=60)

                                                           CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                  >  SRR4302543.6144492/1‑94 (MQ=60)

                                                            AAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAA                                 >  SRR4302543.3211594/1‑94 (MQ=60)

                                                              AGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAC                               >  SRR4302543.2909941/1‑94 (MQ=60)

                                                               GATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGCCC                              >  SRR4302543.2789132/1‑94 (MQ=60)

                                                               GATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACC                              <  SRR4302543.1534489/94‑1 (MQ=60)

                                                               GATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACC                              >  SRR4302543.3478528/1‑94 (MQ=60)

                                                                ATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCA                             >  SRR4302543.708358/1‑94 (MQ=60)

                                                                ATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCA                             >  SRR4302543.7446544/1‑94 (MQ=60)

                                                                 TAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCAT                            <  SRR4302543.1871457/94‑1 (MQ=60)

                                                                 TAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCAT                            >  SRR4302543.2291030/1‑94 (MQ=60)

                                                                  AATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATA                           >  SRR4302543.948391/1‑94 (MQ=60)

                                                                   ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                          <  SRR4302543.2427764/94‑1 (MQ=60)

                                                                   ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                          <  SRR4302543.7986399/94‑1 (MQ=60)

                                                                   ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                          <  SRR4302543.3508412/94‑1 (MQ=60)

                                                                   ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                          <  SRR4302543.2427765/94‑1 (MQ=60)

                                                                     GCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCG                        <  SRR4302543.1731547/94‑1 (MQ=60)

                                                                     GCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCG                        <  SRR4302543.3370054/94‑1 (MQ=60)

                                                                       AAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAA                      >  SRR4302543.715984/1‑94 (MQ=60)

                                                                       AAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAA                      >  SRR4302543.5007180/1‑94 (MQ=60)

                                                                       AAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAA                      >  SRR4302543.3011197/1‑94 (MQ=60)

                                                                        AGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAAC                     >  SRR4302543.4113289/1‑94 (MQ=60)

                                                                         GGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACT                    <  SRR4302543.7863617/94‑1 (MQ=60)

                                                                         GGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACT                    <  SRR4302543.4818138/94‑1 (MQ=60)

                                                                         GGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACT                    >  SRR4302543.5762035/1‑94 (MQ=60)

                                                                          GACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTG                   >  SRR4302543.5312059/1‑94 (MQ=60)

                                                                          GACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTG                   >  SRR4302543.3834936/1‑94 (MQ=60)

                                                                           ACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGA                  <  SRR4302543.3213297/94‑1 (MQ=60)

                                                                             ACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACC                >  SRR4302543.20580/1‑94 (MQ=60)

                                                                             ACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACC                >  SRR4302543.7751850/1‑94 (MQ=60)

                                                                             ACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACC                <  SRR4302543.7540188/94‑1 (MQ=60)

                                                                             ACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACC                >  SRR4302543.3063118/1‑94 (MQ=60)

                                                                              CTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCG               >  SRR4302543.5645959/1‑94 (MQ=60)

                                                                              CTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCG               >  SRR4302543.4579393/1‑94 (MQ=60)

                                                                              CTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCG               >  SRR4302543.747933/1‑94 (MQ=60)

                                                                              CTGACTACGCAGGTCAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTACGCTGACACAGACCATATCGAACTGACCG               >  SRR4302543.4582031/1‑94 (MQ=60)

                                                                               TGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGA              >  SRR4302543.2657466/1‑94 (MQ=60)

                                                                                GACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAG             <  SRR4302543.1989763/94‑1 (MQ=60)

                                                                                GACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAG             <  SRR4302543.6429905/94‑1 (MQ=60)

                                                                                GACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAG             >  SRR4302543.5955516/1‑94 (MQ=60)

                                                                                 ACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGC            <  SRR4302543.1191515/94‑1 (MQ=60)

                                                                                 ACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGC            >  SRR4302543.1338738/1‑94 (MQ=60)

                                                                                 ACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGC            >  SRR4302543.6507993/1‑94 (MQ=60)

                                                                                  CTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCC           >  SRR4302543.5065897/1‑94 (MQ=60)

                                                                                   TACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCA          <  SRR4302543.3702003/94‑1 (MQ=60)

                                                                                   TACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCA          >  SRR4302543.7250987/1‑94 (MQ=60)

                                                                                     CGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGC        <  SRR4302543.5513984/94‑1 (MQ=60)

                                                                                      GCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCT       >  SRR4302543.537963/1‑94 (MQ=60)

                                                                                         GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG    <  SRR4302543.4189968/94‑1 (MQ=60)

                                                                                          GTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGA   >  SRR4302543.433954/1‑94 (MQ=60)

                                                                                          GTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGA   >  SRR4302543.750168/1‑94 (MQ=60)

                                                                                           TGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAG  <  SRR4302543.2185917/94‑1 (MQ=60)

                                                                                           TGAGTCATCCAGGCGGTTAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAG  <  SRR4302543.6435638/94‑1 (MQ=60)

                                                                                           GGAGTCATCCAGCCGGTGAACGCTGCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAG  <  SRR4302543.2185918/94‑1 (MQ=60)

AGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAG  >  NC_000913/2259828‑2260012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`