Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,046,045	T→C	V339V (GTA→GTG)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,046,045	0	T	C	99.6%	868.1 / ‑5.4	275	V339V (GTA→GTG)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (127/146); total (127/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 286 total aligned reads displayed.

ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCC  >  NC_000913/3045955‑3046134

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        >  1:901771/1‑93 (MQ=255)

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        <  2:3710159/93‑1 (MQ=255)

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        >  2:4236807/1‑93 (MQ=255)

  cGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCt                                                                                       <  1:3658741/93‑1 (MQ=255)

   ggTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTg                                                                                      <  2:1596916/93‑1 (MQ=255)

   ggTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTg                                                                                      >  2:1998996/1‑93 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCtcg                                                                                     >  1:3490744/1‑91 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                     <  1:2113762/93‑1 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                     <  1:4671287/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAGCCGGGCCGGGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  1:2055813/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  1:1114534/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  >  2:2971505/1‑93 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  2:3266970/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  1:4209273/93‑1 (MQ=255)

        gACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAg                                                                                 >  2:68511/1‑93 (MQ=255)

           ggTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTAc                                                                              >  2:3996353/1‑93 (MQ=255)

            gTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACa                                                                             <  1:7785152/93‑1 (MQ=255)

             tGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaa                                                                            <  2:167723/93‑1 (MQ=255)

             tGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaa                                                                            <  2:79440/93‑1 (MQ=255)

             tGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaa                                                                            >  1:1070572/1‑93 (MQ=255)

               aaTGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAAt                                                                          >  2:2994947/1‑93 (MQ=255)

                aTGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATg                                                                         <  2:7129416/93‑1 (MQ=255)

                aTGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATg                                                                         >  1:5452706/1‑93 (MQ=255)

                   cGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTg                                                                      <  1:455532/93‑1 (MQ=255)

                   cGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTg                                                                      <  1:736094/93‑1 (MQ=255)

                    ggTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTgg                                                                     >  1:5766032/1‑93 (MQ=255)

                    ggTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTgg                                                                     >  2:2353684/1‑93 (MQ=255)

                     gTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGa                                                                    <  2:7413291/93‑1 (MQ=255)

                     gTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGa                                                                    >  1:1919113/1‑93 (MQ=255)

                      ttAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAg                                                                   >  1:2529822/1‑93 (MQ=255)

                        aGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGtt                                                                 <  2:2176481/93‑1 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                                >  2:63297/1‑93 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                                <  2:3549344/93‑1 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                                >  1:3697126/1‑93 (MQ=255)

                          cGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCg                                                               <  2:6193177/93‑1 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                              >  1:437321/1‑93 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                              >  1:2306509/1‑93 (MQ=255)

                            aTACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                             <  1:1370358/93‑1 (MQ=255)

                            aTACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                             <  1:2685361/93‑1 (MQ=255)

                               cGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                          <  2:454062/93‑1 (MQ=255)

                                gTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                         >  2:669781/1‑93 (MQ=255)

                                gTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                         >  1:324210/1‑93 (MQ=255)

                                  ttCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCa                                                       <  2:7231676/93‑1 (MQ=255)

                                   tCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                      <  1:2648355/93‑1 (MQ=255)

                                        ccAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcgg                                                 <  1:5313306/93‑1 (MQ=255)

                                         cAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGa                                                <  1:1575175/93‑1 (MQ=255)

                                          aaCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGat                                               <  1:6353936/93‑1 (MQ=255)

                                          aaCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGat                                               <  2:504568/93‑1 (MQ=255)

                                           aCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGata                                              <  2:731406/93‑1 (MQ=255)

                                           aCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGata                                              >  1:3969797/1‑93 (MQ=255)

                                            ccGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGatat                                             <  1:4880232/93‑1 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                           <  2:3868729/93‑1 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                           >  2:911866/1‑93 (MQ=255)

                                               ggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                          <  2:4323484/93‑1 (MQ=255)

                                               ggCAGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                          <  2:2744370/93‑1 (MQ=255)

                                                gCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTg                                         >  1:3365500/1‑93 (MQ=255)

                                                gCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTCCGCTTTCTCACGGCGGATATGTTg                                         >  1:2856109/1‑93 (MQ=255)

                                                 gcGTGATAAACGGCATACAGGCTGGCTATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCTCTTTCTCACGGCGGATATGTTGc                                        <  2:7702095/92‑1 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGGTc                                      >  2:4791439/1‑93 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  1:96510/1‑93 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  2:5645001/1‑93 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  1:5625056/1‑93 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  >  2:1193291/1‑93 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  <  1:1297997/93‑1 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  <  1:6732518/93‑1 (MQ=255)

                                                         aaCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                                >  2:3151671/1‑93 (MQ=255)

                                                         aaCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                                >  2:189339/1‑93 (MQ=255)

                                                         aaCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                                <  2:6385897/93‑1 (MQ=255)

                                                          aCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                               <  2:5675840/93‑1 (MQ=255)

                                                          aCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                               <  2:5860804/93‑1 (MQ=255)

                                                          aCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGAAATGTTGTTTGCGAGTc                               >  2:5115647/1‑93 (MQ=255)

                                                           cGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCt                              <  2:3950869/93‑1 (MQ=255)

                                                             gCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGc                            <  1:3130462/93‑1 (MQ=255)

                                                               aTACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                          <  1:3279214/93‑1 (MQ=255)

                                                               aTACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                          >  1:7711171/1‑93 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                         >  1:6790021/1‑93 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                         <  1:2434109/93‑1 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                         <  1:5915057/93‑1 (MQ=255)

                                                                   aGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGcc                      >  1:7324787/1‑93 (MQ=255)

                                                                    ggCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                     <  1:5568144/93‑1 (MQ=255)

                                                                    ggCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                     >  2:1040922/1‑93 (MQ=255)

                                                                    ggCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                     >  1:6509748/1‑93 (MQ=255)

                                                                     gCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCAt                    >  2:2491820/1‑93 (MQ=255)

                                                                      cTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATg                   <  2:3664722/93‑1 (MQ=255)

                                                                          cGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCa               <  1:814252/93‑1 (MQ=255)

                                                                           gATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAg              >  1:2620205/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             <  1:194933/93‑1 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             >  2:2701436/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             >  1:5079898/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             >  2:5022507/1‑93 (MQ=255)

                                                                             tGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCcgcg            >  2:7007987/1‑93 (MQ=255)

                                                                             tGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcg            >  2:7176708/1‑93 (MQ=255)

                                                                               tttGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGcgcg          <  1:5172523/93‑1 (MQ=255)

                                                                               tttGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGcgcg          >  2:7244529/1‑93 (MQ=255)

                                                                                  gCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTa       >  1:6083535/1‑93 (MQ=255)

                                                                                  gCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTa       <  1:6168492/93‑1 (MQ=255)

                                                                                    cagcagCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGAGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAtt     >  1:2392045/1‑93 (MQ=255)

                                                                                     agcagcACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTTACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTg    >  2:7658005/1‑93 (MQ=255)

                                                                                      gcagcaCCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGc   >  2:25683/1‑93 (MQ=255)

                                                                                      gcagcaCCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGc   >  2:1409832/1‑93 (MQ=255)

ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCC  >  NC_000913/3045955‑3046134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCCAGCT  >  NC_000913/3045952‑3046138

GATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGC                                                                                               >  SRR4302543.6932432/1‑94 (MQ=60)

GATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGC                                                                                               <  SRR4302543.6448901/94‑1 (MQ=60)

 ATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCCGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCA                                                                                              >  SRR4302543.4192791/1‑94 (MQ=60)

 ATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCA                                                                                              >  SRR4302543.2532463/1‑94 (MQ=60)

 ATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCA                                                                                              <  SRR4302543.2123823/94‑1 (MQ=60)

   ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACC                                                                                            >  SRR4302543.909724/1‑94 (MQ=60)

    TCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCT                                                                                           <  SRR4302543.7396011/94‑1 (MQ=60)

    TCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCT                                                                                           <  SRR4302543.3720517/94‑1 (MQ=60)

    TCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCT                                                                                           <  SRR4302543.4074920/94‑1 (MQ=60)

    TCGGTCAGACGGTGAATGCGGTTAGCGATACGTTACAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCT                                                                                           <  SRR4302543.5132240/94‑1 (MQ=60)

     CGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTG                                                                                          <  SRR4302543.5230654/94‑1 (MQ=60)

     CGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTG                                                                                          >  SRR4302543.6056054/1‑94 (MQ=60)

     CGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTG                                                                                          <  SRR4302543.5682971/94‑1 (MQ=60)

     CGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTG                                                                                          <  SRR4302543.3690872/94‑1 (MQ=60)

      GGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTCG                                                                                         >  SRR4302543.3521990/1‑94 (MQ=60)

       GTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGG                                                                                        <  SRR4302543.4713867/94‑1 (MQ=60)

       GTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGG                                                                                        <  SRR4302543.2132681/94‑1 (MQ=60)

         CAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAA                                                                                      >  SRR4302543.4671560/1‑94 (MQ=60)

         CAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAA                                                                                      >  SRR4302543.3340964/1‑94 (MQ=60)

         CAGACGGTGAATGCGGGTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAA                                                                                      <  SRR4302543.5135905/94‑1 (MQ=60)

          AGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAGCCGGGCCGGGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAG                                                                                     <  SRR4302543.2074216/94‑1 (MQ=60)

          AGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAG                                                                                     <  SRR4302543.4247437/94‑1 (MQ=60)

          AGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAG                                                                                     <  SRR4302543.2393917/94‑1 (MQ=60)

          AGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAG                                                                                     <  SRR4302543.1124620/94‑1 (MQ=60)

          AGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAG                                                                                     <  SRR4302543.2220398/94‑1 (MQ=60)

            ACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTA                                                                                   <  SRR4302543.904313/94‑1 (MQ=60)

              GGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACA                                                                                 >  SRR4302543.3417765/1‑94 (MQ=60)

              GGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACA                                                                                 >  SRR4302543.3898382/1‑94 (MQ=60)

              GGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACA                                                                                 >  SRR4302543.4539141/1‑94 (MQ=60)

               GTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAA                                                                                >  SRR4302543.346059/1‑94 (MQ=60)

               GTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAA                                                                                >  SRR4302543.1080196/1‑94 (MQ=60)

               GTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAA                                                                                <  SRR4302543.7858546/94‑1 (MQ=60)

                 GAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAAT                                                                              <  SRR4302543.4404483/94‑1 (MQ=60)

                  AATTCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATG                                                                             >  SRR4302543.1365034/1‑94 (MQ=60)

                  AATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATG                                                                             >  SRR4302543.5502151/1‑94 (MQ=60)

                   ATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGT                                                                            <  SRR4302543.2325842/94‑1 (MQ=60)

                   ATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGT                                                                            <  SRR4302543.2657173/94‑1 (MQ=60)

                   ATGCGGTTAGCGATACGTGTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCACCAGCACCTGGGAAGTACAAATGT                                                                            <  SRR4302543.2657174/94‑1 (MQ=60)

                    TGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTT                                                                           <  SRR4302543.1587185/94‑1 (MQ=60)

                     GCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTG                                                                          <  SRR4302543.1966305/94‑1 (MQ=60)

                     GCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTG                                                                          >  SRR4302543.6563168/1‑94 (MQ=60)

                      CGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGG                                                                         <  SRR4302543.742685/94‑1 (MQ=60)

                      CGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGG                                                                         <  SRR4302543.459774/94‑1 (MQ=60)

                      CGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGG                                                                         >  SRR4302543.5818549/1‑94 (MQ=60)

                      CGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGG                                                                         >  SRR4302543.2746707/1‑94 (MQ=60)

                       GGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGA                                                                        >  SRR4302543.1936415/1‑94 (MQ=60)

                        GTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAG                                                                       <  SRR4302543.1193579/94‑1 (MQ=60)

                        GTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAG                                                                       <  SRR4302543.2634306/94‑1 (MQ=60)

                        GTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAG                                                                       >  SRR4302543.2552840/1‑94 (MQ=60)

                          TAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAATGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGCGTAGTACAAATGATGGAGTT                                                                     >  SRR4302543.3221179/1‑94 (MQ=60)

                          TAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTT                                                                     <  SRR4302543.2132477/94‑1 (MQ=60)

                          TAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTT                                                                     <  SRR4302543.6221572/94‑1 (MQ=60)

                           AGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTC                                                                    >  SRR4302543.3729630/1‑94 (MQ=60)

                            GCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCG                                                                   <  SRR4302543.4615196/94‑1 (MQ=60)

                            GCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGAACCTGGGAAGTACAAATGTTGGAGTTCG                                                                   <  SRR4302543.4948643/94‑1 (MQ=60)

                             CGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGC                                                                  >  SRR4302543.2327515/1‑94 (MQ=60)

                             CGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGC                                                                  >  SRR4302543.883736/1‑94 (MQ=60)

                             CGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGC                                                                  >  SRR4302543.441419/1‑94 (MQ=60)

                             CGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGC                                                                  >  SRR4302543.5588870/1‑94 (MQ=60)

                              GATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCT                                                                 >  SRR4302543.3342818/1‑94 (MQ=60)

                               ATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTT                                                                >  SRR4302543.1297258/1‑94 (MQ=60)

                               ATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTT                                                                <  SRR4302543.2709695/94‑1 (MQ=60)

                               ATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTT                                                                <  SRR4302543.1382660/94‑1 (MQ=60)

                                TACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTT                                                               <  SRR4302543.3874319/94‑1 (MQ=60)

                                 ACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTC                                                              <  SRR4302543.3136330/94‑1 (MQ=60)

                                  CGTTTCAGGCCAACCGGGCCGTGATACACGTCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCT                                                             <  SRR4302543.3285282/94‑1 (MQ=60)

                                  CGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCT                                                             >  SRR4302543.1483921/1‑94 (MQ=60)

                                  CGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCT                                                             >  SRR4302543.2423929/1‑94 (MQ=60)

                                  CGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCT                                                             <  SRR4302543.3285283/94‑1 (MQ=60)

                                  CGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCT                                                             >  SRR4302543.327148/1‑94 (MQ=60)

                                   GTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTC                                                            >  SRR4302543.3535332/1‑94 (MQ=60)

                                   GTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTC                                                            <  SRR4302543.6689856/94‑1 (MQ=60)

                                   GTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTC                                                            >  SRR4302543.2591302/1‑94 (MQ=60)

                                   GTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTC                                                            >  SRR4302543.7812760/1‑94 (MQ=60)

                                     TTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTTGCTTTCTCAC                                                          <  SRR4302543.4309639/94‑1 (MQ=60)

                                     TTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAC                                                          <  SRR4302543.5471990/94‑1 (MQ=60)

                                      TCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACG                                                         <  SRR4302543.2672359/94‑1 (MQ=60)

                                       CAGGCCAACCGGGCCGTGATAGACGGCATACACGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCCCGG                                                        >  SRR4302543.5653234/1‑94 (MQ=60)

                                        AGGCCACCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGC                                                       <  SRR4302543.1857598/94‑1 (MQ=60)

                                        AGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGC                                                       <  SRR4302543.7442469/94‑1 (MQ=60)

                                         GGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCG                                                      <  SRR4302543.185349/94‑1 (MQ=60)

                                           CCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGA                                                    <  SRR4302543.5361308/94‑1 (MQ=60)

                                           CCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGA                                                    >  SRR4302543.6374916/1‑94 (MQ=60)

                                            CAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGAT                                                   >  SRR4302543.801270/1‑94 (MQ=60)

                                            CAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGAT                                                   <  SRR4302543.1589231/94‑1 (MQ=60)

                                             AACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATA                                                  <  SRR4302543.6413682/94‑1 (MQ=60)

                                             AACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATA                                                  >  SRR4302543.4004986/1‑94 (MQ=60)

                                              ACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGCTAT                                                 >  SRR4302543.6131238/1‑94 (MQ=60)

                                              ACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATAT                                                 <  SRR4302543.7509560/94‑1 (MQ=60)

                                               CCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATG                                                <  SRR4302543.4924716/94‑1 (MQ=60)

                                                CGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGT                                               <  SRR4302543.3196230/94‑1 (MQ=60)

                                                  GGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTG                                             >  SRR4302543.3395750/1‑94 (MQ=60)

                                                  GGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTG                                             >  SRR4302543.635005/1‑94 (MQ=60)

                                                  GGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTCCGCTTTCTCACGGCGGATATGTTG                                             >  SRR4302543.2882163/1‑94 (MQ=60)

                                                     CGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTC                                          >  SRR4302543.945613/1‑94 (MQ=60)

                                                     CGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTC                                          >  SRR4302543.97410/1‑94 (MQ=60)

                                                     CGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTC                                          >  SRR4302543.5676316/1‑94 (MQ=60)

                                                     CGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTC                                          >  SRR4302543.1113710/1‑94 (MQ=60)

                                                     CGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTC                                          >  SRR4302543.2790861/1‑94 (MQ=60)

                                                     AGTGATAAACGGCATACAGGCTGGAGATGTTTGCCAGCCGCAACTGGGAAGTCCAAAAATTGGAGCTAGCATTCTCACGGCGGATATGTTGCTC                                          >  SRR4302543.97223/1‑94 (MQ=40)

                                                      GTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCG                                         >  SRR4302543.2695153/1‑94 (MQ=60)

                                                      GTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCG                                         >  SRR4302543.976747/1‑94 (MQ=60)

                                                        GATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCG                                       >  SRR4302543.561351/1‑94 (MQ=60)

                                                          TAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAG                                     <  SRR4302543.1309533/94‑1 (MQ=60)

                                                          TAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAG                                     <  SRR4302543.6796120/94‑1 (MQ=60)

                                                           AAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGT                                    >  SRR4302543.5395261/1‑94 (MQ=60)

                                                             ACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCT                                  <  SRR4302543.820311/94‑1 (MQ=60)

                                                             ACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCGGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCT                                  <  SRR4302543.234493/94‑1 (MQ=60)

                                                              CGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTG                                 <  SRR4302543.4125533/94‑1 (MQ=60)

                                                               GGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGC                                >  SRR4302543.3149116/1‑94 (MQ=60)

                                                                GCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCA                               <  SRR4302543.3158618/94‑1 (MQ=60)

                                                                GCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCA                               >  SRR4302543.2473900/1‑94 (MQ=60)

                                                                 CATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAT                              >  SRR4302543.7784114/1‑94 (MQ=60)

                                                                 CATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAT                              >  SRR4302543.2031907/1‑94 (MQ=60)

                                                                  ATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCTCGAGTCTGCATC                             >  SRR4302543.6854142/1‑94 (MQ=60)

                                                                  ATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATC                             <  SRR4302543.3308819/94‑1 (MQ=60)

                                                                  ATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATC                             >  SRR4302543.6854141/1‑94 (MQ=60)

                                                                  ATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATC                             <  SRR4302543.6602246/94‑1 (MQ=60)

                                                                  ATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATC                             >  SRR4302543.7424314/1‑94 (MQ=60)

                                                                  ATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATC                             >  SRR4302543.4805135/1‑94 (MQ=60)

                                                                   TACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCG                            <  SRR4302543.2456073/94‑1 (MQ=60)

                                                                   TACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCG                            <  SRR4302543.4446563/94‑1 (MQ=60)

                                                                   TACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCG                            <  SRR4302543.5969076/94‑1 (MQ=60)

                                                                     CAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCC                          >  SRR4302543.7395319/1‑94 (MQ=60)

                                                                     CAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCC                          <  SRR4302543.5225714/94‑1 (MQ=60)

                                                                      AGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCA                         >  SRR4302543.1351041/1‑94 (MQ=60)

                                                                      AGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCA                         >  SRR4302543.6571069/1‑94 (MQ=60)

                                                                       GGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCAT                        <  SRR4302543.5618806/94‑1 (MQ=60)

                                                                       GGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCAT                        <  SRR4302543.5618805/94‑1 (MQ=60)

                                                                        GCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATG                       <  SRR4302543.7824576/94‑1 (MQ=60)

                                                                        GCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATG                       <  SRR4302543.6526195/94‑1 (MQ=60)

                                                                           GGCGATGTTTGCCAGCAGCACCTGGGTAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTTGCGAGTCTGCATCGCCATGTGC                    >  SRR4302543.4661618/1‑94 (MQ=60)

                                                                            GCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCA                   <  SRR4302543.1946100/94‑1 (MQ=60)

                                                                             CGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAG                  >  SRR4302543.1999934/1‑94 (MQ=60)

                                                                             CGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAG                  <  SRR4302543.821423/94‑1 (MQ=60)

                                                                             CGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAG                  >  SRR4302543.2643948/1‑94 (MQ=60)

                                                                              GATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGC                 >  SRR4302543.5125975/1‑94 (MQ=60)

                                                                               ATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCG                >  SRR4302543.6814268/1‑94 (MQ=60)

                                                                               ATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCG                <  SRR4302543.196579/94‑1 (MQ=60)

                                                                               ATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCG                >  SRR4302543.5895744/1‑94 (MQ=60)

                                                                                TGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGC               <  SRR4302543.3712691/94‑1 (MQ=60)

                                                                                  TTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGG             <  SRR4302543.5219237/94‑1 (MQ=60)

                                                                                    TGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTA           >  SRR4302543.6141192/1‑94 (MQ=60)

                                                                                    TGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTA           <  SRR4302543.2500445/94‑1 (MQ=60)

                                                                                    TGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTA           >  SRR4302543.6141190/1‑94 (MQ=60)

                                                                                     GCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAT          <  SRR4302543.6226853/94‑1 (MQ=60)

                                                                                      CCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGAGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATT         >  SRR4302543.2413690/1‑94 (MQ=60)

                                                                                       CAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTG        <  SRR4302543.2413766/94‑1 (MQ=60)

                                                                                       CAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTG        <  SRR4302543.6887068/94‑1 (MQ=60)

                                                                                        AGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGC       >  SRR4302543.5906664/1‑94 (MQ=60)

                                                                                          CAGTACCTGTGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCTGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCCA     <  SRR4302543.4924805/94‑1 (MQ=60)

                                                                                           AGCACCTGGGAAGGACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGGGTCTGCATCGCTATGCGCAGCGCGGTATTGCCAC    >  SRR4302543.7221553/1‑94 (MQ=60)

                                                                                             CACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCCAGCT  >  SRR4302543.5437353/1‑94 (MQ=60)

GATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCCAGCT  >  NC_000913/3045952‑3046138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`