Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,507	T→C	intergenic (‑182/‑107)	htrL ← / → rfaD	HtrL/ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,507	0	T	C	99.7%	922.4 / ‑4.9	290	intergenic (‑182/‑107)	htrL/rfaD	HtrL/ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (245/44); total (246/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

Only 100 of 292 total aligned reads displayed.

AATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATA  >  NC_000913/3791417‑3791596

aaTATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCAccc                                                                                         >  2:1012962/1‑93 (MQ=255)

 atatTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCt                                                                                        >  1:7072728/1‑93 (MQ=255)

  tatTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCtt                                                                                       >  1:4973894/1‑93 (MQ=255)

  tatTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCtt                                                                                       >  2:1914313/1‑93 (MQ=255)

    ttGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGc                                                                                     >  1:4403638/1‑93 (MQ=255)

       aTACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCAcc                                                                                  >  2:7581244/1‑93 (MQ=255)

         aCTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACctc                                                                                >  2:7167927/1‑93 (MQ=255)

          cTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACctct                                                                               >  2:5275905/1‑93 (MQ=255)

           tAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTa                                                                              >  2:4733136/1‑93 (MQ=255)

           tAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTa                                                                              >  1:7741870/1‑93 (MQ=255)

           tAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTa                                                                              >  1:5298738/1‑93 (MQ=255)

           tAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTa                                                                              >  2:7075657/1‑93 (MQ=255)

            aaaGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTAt                                                                             >  2:5857559/1‑93 (MQ=255)

                  cTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAg                                                                       >  2:2931505/1‑93 (MQ=255)

                   tATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAgg                                                                      >  1:6885769/1‑93 (MQ=255)

                   tATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAgg                                                                      >  2:5950984/1‑93 (MQ=255)

                   tATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAgg                                                                      >  1:4076549/1‑93 (MQ=255)

                   tATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAgg                                                                      >  2:6600914/1‑93 (MQ=255)

                    aTTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAggg                                                                     >  2:1178266/1‑93 (MQ=255)

                    aTTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAggg                                                                     >  1:1351805/1‑93 (MQ=255)

                     ttCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGc                                                                    >  2:4899952/1‑93 (MQ=255)

                       cacaTGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTg                                                                  >  1:6909170/1‑93 (MQ=255)

                           tGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAgg                                                              >  2:4853760/1‑93 (MQ=255)

                           tGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAgg                                                              <  2:5764053/93‑1 (MQ=255)

                           tGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAgg                                                              >  2:7253635/1‑93 (MQ=255)

                              aaaaCCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAt                                                           >  1:6302099/1‑93 (MQ=255)

                              aaaaCCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAt                                                           >  2:3795495/1‑93 (MQ=255)

                              aaaaCCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAt                                                           >  2:407441/1‑93 (MQ=255)

                               aaaCCAACATCCGCCATGGAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAtt                                                          >  2:7742835/1‑93 (MQ=255)

                               aaaCCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAtt                                                          <  1:1929006/93‑1 (MQ=255)

                                aaCCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTc                                                         >  1:2882966/1‑93 (MQ=255)

                                 aCCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCg                                                        >  2:1833824/1‑93 (MQ=255)

                                    aaCATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAt                                                     >  2:4504449/1‑93 (MQ=255)

                                     aCATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAtg                                                    >  2:419436/1‑93 (MQ=255)

                                     aCATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAtg                                                    >  1:5430121/1‑93 (MQ=255)

                                      cATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAtgt                                                   >  1:2812927/1‑93 (MQ=255)

                                       aTCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAtgtg                                                  >  1:6663811/1‑93 (MQ=255)

                                       aTCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAtgtg                                                  >  2:5986287/1‑93 (MQ=255)

                                       aTCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAtgtg                                                  >  1:1544672/1‑93 (MQ=255)

                                        tCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGa                                                 >  1:5077859/1‑93 (MQ=255)

                                        tCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGa                                                 >  1:5924306/1‑93 (MQ=255)

                                        tCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGa                                                 >  2:206496/1‑93 (MQ=255)

                                          cGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATg                                               >  1:7424482/1‑93 (MQ=255)

                                          cGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATg                                               >  2:6449705/1‑93 (MQ=255)

                                            ccATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGt                                             >  1:4730237/1‑93 (MQ=255)

                                            ccATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGt                                             >  1:2144744/1‑93 (MQ=255)

                                              aTGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTAt                                           >  2:2311202/1‑93 (MQ=255)

                                               tGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATg                                          >  1:6092211/1‑93 (MQ=255)

                                                gAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATgt                                         >  1:1798339/1‑92 (MQ=255)

                                                gAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGa                                         >  1:2693565/1‑93 (MQ=255)

                                                  aGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGAtt                                       >  2:150178/1‑93 (MQ=255)

                                                  aGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGAtt                                       >  1:311580/1‑93 (MQ=255)

                                                   ggACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTa                                      <  1:576291/93‑1 (MQ=255)

                                                    gACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTaa                                     >  2:1381496/1‑92 (MQ=255)

                                                     aCTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACa                                    <  2:1356157/93‑1 (MQ=255)

                                                     aCTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACa                                    <  2:5632444/93‑1 (MQ=255)

                                                       tAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGa                                  >  1:2943626/1‑93 (MQ=255)

                                                       tAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGa                                  >  2:2585875/1‑93 (MQ=255)

                                                       tAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGa                                  >  2:3192284/1‑93 (MQ=255)

                                                          cTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACAt                               >  1:926158/1‑93 (MQ=255)

                                                           tAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACAtt                              >  1:3814200/1‑93 (MQ=255)

                                                            aaaaCCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTc                             >  1:2329203/1‑93 (MQ=255)

                                                              aaCCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCgt                           >  2:3551051/1‑93 (MQ=255)

                                                               aCCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCgtg                          >  2:1303427/1‑93 (MQ=255)

                                                               aCCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCgtg                          >  2:554360/1‑93 (MQ=255)

                                                                 ccAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTc                        >  1:373817/1‑93 (MQ=255)

                                                                  cAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCt                       >  2:6233792/1‑93 (MQ=255)

                                                                  cAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCt                       >  2:6836481/1‑93 (MQ=255)

                                                                  cAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCt                       >  2:4412253/1‑93 (MQ=255)

                                                                   aaaCTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTg                      >  2:3262814/1‑93 (MQ=255)

                                                                   aaaCTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTg                      >  2:2235552/1‑93 (MQ=255)

                                                                    aaCTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTga                     >  1:1428792/1‑93 (MQ=255)

                                                                     aCTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTgag                    >  2:3055181/1‑93 (MQ=255)

                                                                      cTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTgaga                   >  1:5046099/1‑93 (MQ=255)

                                                                      cTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTgaga                   <  1:4153584/93‑1 (MQ=255)

                                                                      cTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTgaga                   <  1:6465753/93‑1 (MQ=255)

                                                                       tAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAt                  >  1:6748981/1‑93 (MQ=255)

                                                                       tAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAt                  >  2:7662547/1‑93 (MQ=255)

                                                                       tAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAt                  <  2:6522610/93‑1 (MQ=255)

                                                                       tAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAt                  >  2:2835949/1‑93 (MQ=255)

                                                                       tAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAt                  >  2:6911869/1‑93 (MQ=255)

                                                                        aGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAtt                 >  1:3464612/1‑93 (MQ=255)

                                                                         gTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTg                >  1:7581521/1‑93 (MQ=255)

                                                                         gTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTg                >  2:7425001/1‑93 (MQ=255)

                                                                          tttGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGt               >  2:5153984/1‑93 (MQ=255)

                                                                           ttgttgCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGtc              >  2:1644061/1‑93 (MQ=255)

                                                                            tgttgCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGtct             >  1:3009435/1‑93 (MQ=255)

                                                                             gttgCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGtctc            >  2:2978766/1‑93 (MQ=255)

                                                                             gttgCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGtctc            <  1:7146010/93‑1 (MQ=255)

                                                                              ttgCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTctct           <  2:5289141/93‑1 (MQ=255)

                                                                              ttgCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTctct           >  2:2704028/1‑93 (MQ=255)

                                                                              ttgCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTctct           >  2:1792894/1‑93 (MQ=255)

                                                                                gCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGa         <  1:2220648/93‑1 (MQ=255)

                                                                                gCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGa         >  1:3231919/1‑93 (MQ=255)

                                                                                  aaTTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACt       >  1:5695597/1‑93 (MQ=255)

                                                                                   aTTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTc      >  2:5672205/1‑93 (MQ=255)

                                                                                    ttAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTcc     >  1:1230799/1‑93 (MQ=255)

                                                                                    ttAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTcc     <  2:1203546/93‑1 (MQ=255)

                                                                                       gCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATa  <  1:1012962/93‑1 (MQ=255)

                                                                                       gCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATa  >  2:7868289/1‑93 (MQ=255)

AATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATA  >  NC_000913/3791417‑3791596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTC  >  NC_000913/3791414‑3791600

CGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCAC                                                                                               >  SRR4302543.4567196/1‑94 (MQ=60)

   AATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCT                                                                                            >  SRR4302543.7141398/1‑94 (MQ=60)

   AATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCT                                                                                            >  SRR4302543.3542839/1‑94 (MQ=60)

   AATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCT                                                                                            >  SRR4302543.3542840/1‑94 (MQ=60)

    ATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTT                                                                                           >  SRR4302543.5019167/1‑94 (MQ=60)

    ATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTT                                                                                           >  SRR4302543.810170/1‑94 (MQ=60)

     TATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTG                                                                                          >  SRR4302543.7592929/1‑94 (MQ=60)

     TATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTG                                                                                          >  SRR4302543.1884147/1‑94 (MQ=60)

      ATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGC                                                                                         >  SRR4302543.5090937/1‑94 (MQ=60)

      ATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGC                                                                                         >  SRR4302543.2826731/1‑94 (MQ=60)

      ATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGC                                                                                         >  SRR4302543.4443558/1‑94 (MQ=60)

      ATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGC                                                                                         >  SRR4302543.6145614/1‑94 (MQ=60)

        TGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCAC                                                                                       >  SRR4302543.5396647/1‑94 (MQ=60)

         GATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACC                                                                                      >  SRR4302543.3369387/1‑94 (MQ=60)

          ATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCT                                                                                     >  SRR4302543.1548078/1‑94 (MQ=60)

          ATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCT                                                                                     >  SRR4302543.2122212/1‑94 (MQ=60)

          ATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCT                                                                                     >  SRR4302543.1820198/1‑94 (MQ=60)

             CTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTA                                                                                  >  SRR4302543.7168479/1‑94 (MQ=60)

             CTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTA                                                                                  >  SRR4302543.5048945/1‑94 (MQ=60)

             CTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTA                                                                                  >  SRR4302543.5346569/1‑94 (MQ=60)

             CTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTA                                                                                  >  SRR4302543.7815000/1‑94 (MQ=60)

              TAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTAT                                                                                 >  SRR4302543.3772286/1‑94 (MQ=60)

               AAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATG                                                                                >  SRR4302543.7854670/1‑94 (MQ=60)

                AAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGT                                                                               >  SRR4302543.1029542/1‑94 (MQ=60)

                 AGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTA                                                                              >  SRR4302543.4562274/1‑94 (MQ=60)

                 AGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTA                                                                              >  SRR4302543.1594241/1‑94 (MQ=60)

                 AGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTA                                                                              >  SRR4302543.3420685/1‑94 (MQ=60)

                   CACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAA                                                                            >  SRR4302543.3242615/1‑94 (MQ=60)

                     CTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGG                                                                          >  SRR4302543.6822142/1‑94 (MQ=60)

                     CTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGG                                                                          <  SRR4302543.5259068/94‑1 (MQ=60)

                     CTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGG                                                                          >  SRR4302543.4113092/1‑94 (MQ=60)

                     CTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGG                                                                          >  SRR4302543.2874847/1‑94 (MQ=60)

                     CTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGG                                                                          >  SRR4302543.5122172/1‑94 (MQ=60)

                     CTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGG                                                                          >  SRR4302543.6950539/1‑94 (MQ=60)

                     CTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGG                                                                          >  SRR4302543.579142/1‑94 (MQ=60)

                     CTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGCTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGG                                                                          >  SRR4302543.7525394/1‑94 (MQ=60)

                      TATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGG                                                                         >  SRR4302543.1363898/1‑94 (MQ=60)

                      TATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGG                                                                         <  SRR4302543.1760042/94‑1 (MQ=60)

                       ATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGC                                                                        >  SRR4302543.1302001/1‑94 (MQ=60)

                       ATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGC                                                                        >  SRR4302543.3055105/1‑94 (MQ=60)

                        TTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCT                                                                       >  SRR4302543.6414293/1‑94 (MQ=60)

                         TCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTG                                                                      >  SRR4302543.6974105/1‑94 (MQ=60)

                            CATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGCTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAG                                                                   >  SRR4302543.2309048/1‑94 (MQ=60)

                             TTGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGG                                                                  >  SRR4302543.6992224/1‑94 (MQ=60)

                               GCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGA                                                                >  SRR4302543.5370181/1‑94 (MQ=60)

                                CAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAT                                                               >  SRR4302543.7695326/1‑94 (MQ=60)

                                CAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAT                                                               <  SRR4302543.6117990/94‑1 (MQ=60)

                                CAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAT                                                               >  SRR4302543.6361397/1‑94 (MQ=60)

                                CAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAT                                                               >  SRR4302543.588786/1‑94 (MQ=60)

                                CAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGAT                                                               >  SRR4302543.2192309/1‑94 (MQ=60)

                                 AAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATT                                                              >  SRR4302543.6188987/1‑94 (MQ=60)

                                  AAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTC                                                             >  SRR4302543.2909201/1‑94 (MQ=60)

                                  AAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTC                                                             <  SRR4302543.1946371/94‑1 (MQ=60)

                                     CCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGA                                                          >  SRR4302543.1372291/1‑94 (MQ=60)

                                     CCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGA                                                          >  SRR4302543.2339767/1‑94 (MQ=60)

                                      CAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAT                                                         >  SRR4302543.7054210/1‑94 (MQ=60)

                                      CAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAT                                                         <  SRR4302543.5972234/94‑1 (MQ=60)

                                       AACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATG                                                        >  SRR4302543.5479458/1‑94 (MQ=60)

                                        ACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGT                                                       >  SRR4302543.2838692/1‑94 (MQ=60)

                                         CATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTG                                                      >  SRR4302543.1558503/1‑94 (MQ=60)

                                         CATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTG                                                      >  SRR4302543.6726727/1‑94 (MQ=60)

                                          ATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGA                                                     >  SRR4302543.5978442/1‑94 (MQ=60)

                                          ATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGA                                                     >  SRR4302543.6964467/1‑94 (MQ=60)

                                          ATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGA                                                     >  SRR4302543.5123929/1‑94 (MQ=60)

                                            CCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATG                                                   >  SRR4302543.7495687/1‑94 (MQ=60)

                                             CGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGAGGG                                                  >  SRR4302543.4156331/1‑94 (MQ=60)

                                              GCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGT                                                 >  SRR4302543.3890652/1‑94 (MQ=60)

                                              GCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGT                                                 >  SRR4302543.2163947/1‑94 (MQ=60)

                                              GCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGT                                                 >  SRR4302543.6786103/1‑94 (MQ=60)

                                              GCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGT                                                 >  SRR4302543.4773449/1‑94 (MQ=60)

                                               CCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTT                                                >  SRR4302543.5566711/1‑94 (MQ=60)

                                               CCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTA                                                >  SRR4302543.5566712/1‑94 (MQ=60)

                                                 ATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATG                                              >  SRR4302543.6149950/1‑94 (MQ=60)

                                                 ATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATG                                              <  SRR4302543.5284440/94‑1 (MQ=60)

                                                 ATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATG                                              >  SRR4302543.987096/1‑94 (MQ=60)

                                                 ATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATG                                              <  SRR4302543.1332311/94‑1 (MQ=60)

                                                  TGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGT                                             >  SRR4302543.1814443/1‑94 (MQ=60)

                                                  TGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGA                                             >  SRR4302543.4832557/1‑94 (MQ=60)

                                                  TGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGA                                             >  SRR4302543.716333/1‑94 (MQ=60)

                                                  TGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGA                                             >  SRR4302543.2717987/1‑94 (MQ=60)

                                                    AAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATT                                           >  SRR4302543.314390/1‑94 (MQ=60)

                                                      GGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTAC                                         <  SRR4302543.581559/94‑1 (MQ=60)

                                                       GACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACA                                        >  SRR4302543.4821323/1‑94 (MQ=60)

                                                         CTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGA                                      >  SRR4302543.2970354/1‑94 (MQ=60)

                                                         CTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGA                                      >  SRR4302543.969517/1‑94 (MQ=60)

                                                          TAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGAC                                     >  SRR4302543.437297/1‑94 (MQ=60)

                                                            GCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACAT                                   >  SRR4302543.4139392/1‑94 (MQ=60)

                                                            GCTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACAT                                   >  SRR4302543.934256/1‑94 (MQ=60)

                                                             CTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATT                                  >  SRR4302543.3847824/1‑94 (MQ=60)

                                                             CTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATT                                  >  SRR4302543.7692195/1‑94 (MQ=60)

                                                             CTAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATT                                  >  SRR4302543.7470332/1‑94 (MQ=60)

                                                              TAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTC                                 <  SRR4302543.388047/94‑1 (MQ=60)

                                                              TAAAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTC                                 >  SRR4302543.2350354/1‑94 (MQ=60)

                                                                AAACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGT                               >  SRR4302543.2019977/1‑94 (MQ=60)

                                                                 AACCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTG                              <  SRR4302543.2859918/94‑1 (MQ=60)

                                                                   CCCAAGCTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTC                            >  SRR4302543.2801729/1‑94 (MQ=60)

                                                                   CCCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTC                            >  SRR4302543.377257/1‑94 (MQ=60)

                                                                    CCAAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCT                           <  SRR4302543.676421/94‑1 (MQ=60)

                                                                      AAACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGA                         >  SRR4302543.1441717/1‑94 (MQ=60)

                                                                       AACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGTATGTGATGGAATGATTACAGACATTCGTTTTTGAG                        >  SRR4302543.6696471/1‑94 (MQ=60)

                                                                       AACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAG                        >  SRR4302543.5788170/1‑94 (MQ=60)

                                                                       AACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAG                        >  SRR4302543.6404137/1‑94 (MQ=60)

                                                                       AACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAG                        >  SRR4302543.6696472/1‑94 (MQ=60)

                                                                        ACTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGA                       >  SRR4302543.5091983/1‑94 (MQ=60)

                                                                         CTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAT                      <  SRR4302543.4191139/94‑1 (MQ=60)

                                                                         CTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAT                      <  SRR4302543.6526672/94‑1 (MQ=60)

                                                                         CTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAT                      <  SRR4302543.2605369/94‑1 (MQ=60)

                                                                         CTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAT                      >  SRR4302543.6812715/1‑94 (MQ=60)

                                                                         CTAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAT                      >  SRR4302543.6825218/1‑94 (MQ=60)

                                                                          TAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATT                     >  SRR4302543.3495645/1‑94 (MQ=60)

                                                                          TAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATT                     >  SRR4302543.2381544/1‑94 (MQ=60)

                                                                          TAGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATT                     <  SRR4302543.4617565/94‑1 (MQ=60)

                                                                           AGTTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTG                    >  SRR4302543.7653592/1‑94 (MQ=60)

                                                                             TTTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTC                  >  SRR4302543.2195529/1‑94 (MQ=60)

                                                                              TTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCT                 >  SRR4302543.2966955/1‑94 (MQ=60)

                                                                              TTGTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCT                 >  SRR4302543.3036691/1‑94 (MQ=60)

                                                                                GTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCT               <  SRR4302543.3826139/94‑1 (MQ=60)

                                                                                GTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCT               <  SRR4302543.7452757/94‑1 (MQ=60)

                                                                                GTTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCT               <  SRR4302543.7215463/94‑1 (MQ=60)

                                                                                 TTGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTG              <  SRR4302543.3107082/94‑1 (MQ=60)

                                                                                  TGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGA             >  SRR4302543.3261149/1‑94 (MQ=60)

                                                                                  TGCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGA             >  SRR4302543.3261150/1‑94 (MQ=60)

                                                                                   GCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGAC            <  SRR4302543.2240684/94‑1 (MQ=60)

                                                                                   GCAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGAC            <  SRR4302543.1436738/94‑1 (MQ=60)

                                                                                    CAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACT           >  SRR4302543.2992715/1‑94 (MQ=60)

                                                                                    CAATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACT           >  SRR4302543.5747559/1‑94 (MQ=60)

                                                                                     AATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTC          >  SRR4302543.4269834/1‑94 (MQ=60)

                                                                                      ATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCC         >  SRR4302543.3026787/1‑94 (MQ=60)

                                                                                      ATTAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCC         >  SRR4302543.1241859/1‑94 (MQ=60)

                                                                                        TAGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCAT       <  SRR4302543.6271357/94‑1 (MQ=60)

                                                                                         AGCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATA      >  SRR4302543.1545700/1‑94 (MQ=60)

                                                                                          GCACCCTTGCACCTCTATGTAAAGGGCTGAAGGTATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAA     >  SRR4302543.7273553/1‑94 (MQ=60)

                                                                                          GCACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAA     <  SRR4302543.1021925/94‑1 (MQ=60)

                                                                                           CACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAAT    <  SRR4302543.5262838/94‑1 (MQ=60)

                                                                                            ACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATT   >  SRR4302543.6925528/1‑94 (MQ=60)

                                                                                            ACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATT   >  SRR4302543.6925527/1‑94 (MQ=60)

                                                                                            ACCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGCATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATT   <  SRR4302543.5420595/94‑1 (MQ=60)

                                                                                            ACCCTTGCAACTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATT   >  SRR4302543.4977246/1‑94 (MQ=60)

                                                                                             CCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGTTATGATTACAGACATTCTTGTCTTTGGTTGTCTCTTACTCCATAggtc  >  SRR4302543.3847440/1‑90 (MQ=60)

                                                                                             CCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTC  <  SRR4302543.7585134/94‑1 (MQ=60)

                                                                                             CCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTC  <  SRR4302543.7984995/94‑1 (MQ=60)

                                                                                             CCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTC  >  SRR4302543.3096570/1‑94 (MQ=60)

CGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTC  >  NC_000913/3791414‑3791600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`