Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,076,069 T→C intergenic (+51/‑775) yiiD → / → yiiE putative acetyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,076,0690TC99.6% 728.7 / NA 230intergenic (+51/‑775)yiiD/yiiEputative acetyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (78/151);  total (78/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCAG  >  NC_000913/4075979‑4076158
                                                                                          |                                                                                         
aTTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGccc                                                                                         >  2:2959051/1‑93 (MQ=255)
aTTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGccc                                                                                         >  2:4154695/1‑93 (MQ=255)
 tttGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCt                                                                                        <  1:6419095/93‑1 (MQ=255)
 tttGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCt                                                                                        <  2:6410240/93‑1 (MQ=255)
  ttGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCtt                                                                                       <  2:5084669/93‑1 (MQ=255)
  ttGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCtt                                                                                       >  1:7689249/1‑93 (MQ=255)
     gCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGc                                                                                    >  1:1838622/1‑93 (MQ=255)
     gCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGc                                                                                    <  1:469887/93‑1 (MQ=255)
       ccGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTa                                                                                  <  2:3155434/93‑1 (MQ=255)
        cGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTAt                                                                                 >  1:3926340/1‑93 (MQ=255)
         gTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTAtt                                                                                <  2:7839317/93‑1 (MQ=255)
         gTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTAtt                                                                                >  1:7267218/1‑93 (MQ=255)
           aTGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTc                                                                              <  1:7546464/93‑1 (MQ=255)
           aTGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTc                                                                              <  1:4365922/93‑1 (MQ=255)
           aTGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTc                                                                              <  2:478275/93‑1 (MQ=255)
           aTGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTc                                                                              <  2:2736386/93‑1 (MQ=255)
           aTGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTc                                                                              <  1:4084291/93‑1 (MQ=255)
            tGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCg                                                                             >  1:501618/1‑93 (MQ=255)
            tGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCg                                                                             <  1:4816274/93‑1 (MQ=255)
             gAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCgg                                                                            >  1:5724417/1‑93 (MQ=255)
              aaGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCGGGTCATTTATATTAGCCCTTGGCTATTTCGGt                                                                           <  1:1092522/93‑1 (MQ=255)
              aaGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGt                                                                           <  2:1000285/93‑1 (MQ=255)
                gagGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTta                                                                         <  2:4456956/93‑1 (MQ=255)
                gagGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTta                                                                         <  1:396402/93‑1 (MQ=255)
                 agGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTtat                                                                        >  1:2135778/1‑93 (MQ=255)
                 agGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTtat                                                                        >  2:1989735/1‑93 (MQ=255)
                  gggCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTtata                                                                       <  2:1808973/93‑1 (MQ=255)
                  gggCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTtata                                                                       <  2:5471180/93‑1 (MQ=255)
                   ggCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTatat                                                                      <  2:1012267/93‑1 (MQ=255)
                   ggCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTatat                                                                      <  1:2561517/93‑1 (MQ=255)
                    gCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATAtt                                                                     <  1:4464891/93‑1 (MQ=255)
                     cGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTa                                                                    <  2:7647314/93‑1 (MQ=255)
                     cGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTa                                                                    <  2:2123165/93‑1 (MQ=255)
                      gggAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTaa                                                                   <  1:3166878/93‑1 (MQ=255)
                      gggAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTaa                                                                   <  2:3596983/93‑1 (MQ=255)
                       ggAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTaaa                                                                  <  2:4121922/93‑1 (MQ=255)
                        gAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAAt                                                                 <  1:5216116/93‑1 (MQ=255)
                        gAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAAt                                                                 <  1:1156793/93‑1 (MQ=255)
                            gaagaaGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGa                                                             <  1:7571359/93‑1 (MQ=255)
                            gaagaaGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGa                                                             <  2:7462850/93‑1 (MQ=255)
                            gaagaaGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGa                                                             >  2:6337445/1‑93 (MQ=255)
                             aagaagAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGaa                                                            <  1:3728311/93‑1 (MQ=255)
                             aagaagAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGaa                                                            <  1:4208097/93‑1 (MQ=255)
                             aagaagAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGaa                                                            >  2:1022052/1‑93 (MQ=255)
                              agaagaGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAAc                                                           >  1:4228303/1‑93 (MQ=255)
                               gaagaGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAAct                                                          >  1:6500775/1‑93 (MQ=255)
                                aagaGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAActc                                                         <  1:3527057/93‑1 (MQ=255)
                                aagaGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAActc                                                         <  1:7302492/93‑1 (MQ=255)
                                 agagTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAActct                                                        <  1:1356389/93‑1 (MQ=255)
                                  gagTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCta                                                       <  1:7081245/93‑1 (MQ=255)
                                  gagTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCta                                                       <  1:1567551/93‑1 (MQ=255)
                                  gagTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCta                                                       <  2:34765/93‑1 (MQ=255)
                                   agTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCtat                                                      <  1:795221/93‑1 (MQ=255)
                                    gTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTACCCCTTGGCTATTTTGGTTATATTAAATAAGAACTCtata                                                     >  1:6825354/1‑93 (MQ=255)
                                     tAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATaa                                                    <  2:819613/93‑1 (MQ=255)
                                     tAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATaa                                                    >  1:7134844/1‑93 (MQ=255)
                                      aGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATaaa                                                   <  1:6742913/93‑1 (MQ=255)
                                      aGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATaaa                                                   <  1:2756014/93‑1 (MQ=255)
                                      aGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATaaa                                                   <  1:2522437/93‑1 (MQ=255)
                                      aGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATaaa                                                   <  1:5851443/93‑1 (MQ=255)
                                        cTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAtt                                                 <  1:5108152/93‑1 (MQ=255)
                                        cTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAtt                                                 <  2:1850847/93‑1 (MQ=255)
                                          gATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAtttt                                               <  2:6007036/93‑1 (MQ=255)
                                          gATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAtttt                                               >  1:2666258/1‑93 (MQ=255)
                                           aTGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAttttt                                              >  1:4501766/1‑93 (MQ=255)
                                              gagaGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTAtt                                           <  1:1015943/93‑1 (MQ=255)
                                               agagTGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTAttc                                          <  2:6969109/93‑1 (MQ=255)
                                                gagGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTAttct                                         <  1:2172431/93‑1 (MQ=255)
                                                gagGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTAttct                                         <  1:3814464/93‑1 (MQ=255)
                                                    ggCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGt                                     <  1:6001904/93‑1 (MQ=255)
                                                     gCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGtt                                    <  2:2433985/93‑1 (MQ=255)
                                                      cGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTg                                   <  2:1630925/93‑1 (MQ=255)
                                                       ggTGCTACCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTATATAACTTTTTATTCTTCGTTgg                                  <  2:7009895/93‑1 (MQ=255)
                                                         tgctgcCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCt                                >  1:3411066/1‑93 (MQ=255)
                                                           ctgcCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTcc                              <  2:6777299/93‑1 (MQ=255)
                                                           ctgcCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTcc                              <  2:5206433/93‑1 (MQ=255)
                                                               ctctctCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATAc                          <  2:6171643/93‑1 (MQ=255)
                                                                tctctcATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACg                         >  2:3722698/1‑93 (MQ=255)
                                                                tctctcATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACg                         >  2:5575666/1‑93 (MQ=255)
                                                                 ctctcATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgt                        >  2:1419860/1‑93 (MQ=255)
                                                                      atccAGGTCATTTATAGTAGCCCTTTGATATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTTTCtt                   <  1:407845/90‑1 (MQ=255)
                                                                      aTTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCtt                   <  1:6107716/93‑1 (MQ=255)
                                                                      aTTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCtt                   >  1:2948119/1‑93 (MQ=255)
                                                                       ttCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTg                  >  1:4577612/1‑93 (MQ=255)
                                                                        tCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGc                 <  2:22465/93‑1 (MQ=255)
                                                                          aGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTa               <  2:6642150/93‑1 (MQ=255)
                                                                              cATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATc           >  1:3567863/1‑93 (MQ=255)
                                                                              cATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATc           >  2:1497667/1‑93 (MQ=255)
                                                                                tttATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCAc         >  2:2937496/1‑93 (MQ=255)
                                                                                tttATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCAc         <  2:2537577/93‑1 (MQ=255)
                                                                                tttATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCAc         >  1:4395078/1‑93 (MQ=255)
                                                                                   atatTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGt      <  2:5456707/93‑1 (MQ=255)
                                                                                   atatTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGt      <  2:3247012/93‑1 (MQ=255)
                                                                                    tatTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGtt     >  1:7871995/1‑93 (MQ=255)
                                                                                    tatTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGtt     >  1:5512510/1‑93 (MQ=255)
                                                                                     atTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTc    >  1:6837642/1‑93 (MQ=255)
                                                                                      ttAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCa   <  2:6305974/93‑1 (MQ=255)
                                                                                      ttAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCa   <  2:4885574/93‑1 (MQ=255)
                                                                                      ttAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCa   >  2:7236989/1‑93 (MQ=255)
                                                                                       tAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCAg  <  2:2875526/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCAG  >  NC_000913/4075979‑4076158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCAGGAG  >  NC_000913/4075977‑4076161
                                                                                            |                                                                                            
CCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCC                                                                                             <  SRR4302543.2324222/94‑1 (MQ=60)
  ATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCT                                                                                           >  SRR4302543.1033135/1‑94 (MQ=60)
  ATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCT                                                                                           <  SRR4302543.1103950/94‑1 (MQ=60)
   TTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTT                                                                                          <  SRR4302543.6479564/94‑1 (MQ=60)
   TTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTT                                                                                          <  SRR4302543.4255229/94‑1 (MQ=60)
   TTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTT                                                                                          >  SRR4302543.7762058/1‑94 (MQ=60)
    TTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGGGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTG                                                                                         <  SRR4302543.6819265/94‑1 (MQ=60)
    TTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTG                                                                                         <  SRR4302543.5358663/94‑1 (MQ=60)
     TGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGG                                                                                        <  SRR4302543.5525265/94‑1 (MQ=60)
      GGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGC                                                                                       >  SRR4302543.1855158/1‑94 (MQ=60)
       GCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCT                                                                                      >  SRR4302543.2805993/1‑94 (MQ=60)
       GCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCT                                                                                      <  SRR4302543.474251/94‑1 (MQ=60)
         CCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTAT                                                                                    <  SRR4302543.433170/94‑1 (MQ=60)
         CCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTAT                                                                                    >  SRR4302543.3960997/1‑94 (MQ=60)
          CGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATT                                                                                   >  SRR4302543.7337429/1‑94 (MQ=60)
            TATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTC                                                                                 >  SRR4302543.7057392/1‑94 (MQ=60)
            TATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTC                                                                                 <  SRR4302543.4928665/94‑1 (MQ=60)
             ATGAAGAGGGCGGGAACGAAGAAGATTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCG                                                                                <  SRR4302543.1723045/94‑1 (MQ=60)
             ATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTTCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCG                                                                                <  SRR4302543.4405471/94‑1 (MQ=60)
             ATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTCTTTCG                                                                                >  SRR4302543.506271/1‑94 (MQ=60)
             ATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCG                                                                                <  SRR4302543.4120907/94‑1 (MQ=60)
             ATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCG                                                                                <  SRR4302543.3548271/94‑1 (MQ=60)
             ATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCG                                                                                <  SRR4302543.7618361/94‑1 (MQ=60)
             ATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCG                                                                                >  SRR4302543.506269/1‑94 (MQ=60)
             ATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCG                                                                                <  SRR4302543.4405470/94‑1 (MQ=60)
              TGAAGAGGGCGGGAACGAAGACGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGG                                                                               <  SRR4302543.7906572/94‑1 (MQ=60)
              TGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGG                                                                               >  SRR4302543.5776652/1‑94 (MQ=60)
              TGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGG                                                                               <  SRR4302543.4680226/94‑1 (MQ=60)
              TGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGG                                                                               <  SRR4302543.4860248/94‑1 (MQ=60)
               GAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGT                                                                              >  SRR4302543.820142/1‑94 (MQ=60)
               GAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGT                                                                              <  SRR4302543.7233306/94‑1 (MQ=60)
               GAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGACATTTATATTAGCCCTTCGCTATTTCGGT                                                                              >  SRR4302543.3155121/1‑94 (MQ=60)
                AAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCGGGTCATTTATATTAGCCCTTGGCTATTTCGGTT                                                                             <  SRR4302543.1102346/94‑1 (MQ=60)
                AAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTT                                                                             >  SRR4302543.7389598/1‑94 (MQ=60)
                 AGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTA                                                                            >  SRR4302543.602135/1‑94 (MQ=60)
                  GAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTAT                                                                           <  SRR4302543.7216652/94‑1 (MQ=60)
                  GAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTAT                                                                           <  SRR4302543.400064/94‑1 (MQ=60)
                  GAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTAT                                                                           >  SRR4302543.2154889/1‑94 (MQ=60)
                  GAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTAT                                                                           >  SRR4302543.4930109/1‑94 (MQ=60)
                  GAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTAT                                                                           <  SRR4302543.1517114/94‑1 (MQ=60)
                    GGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATAT                                                                         <  SRR4302543.4207198/94‑1 (MQ=60)
                     GGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATT                                                                        <  SRR4302543.656384/94‑1 (MQ=60)
                     GGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATT                                                                        <  SRR4302543.2584924/94‑1 (MQ=60)
                      GCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTA                                                                       <  SRR4302543.4505359/94‑1 (MQ=60)
                      GCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTA                                                                       <  SRR4302543.2688974/94‑1 (MQ=60)
                        GGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAA                                                                     <  SRR4302543.6308747/94‑1 (MQ=60)
                        GGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAA                                                                     <  SRR4302543.3195394/94‑1 (MQ=60)
                         GGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAAT                                                                    <  SRR4302543.4011662/94‑1 (MQ=60)
                          GAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATA                                                                   <  SRR4302543.5263106/94‑1 (MQ=60)
                          GAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATA                                                                   <  SRR4302543.5263105/94‑1 (MQ=60)
                          GAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATA                                                                   <  SRR4302543.1167324/94‑1 (MQ=60)
                            ACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAG                                                                 <  SRR4302543.6327372/94‑1 (MQ=60)
                            ACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAG                                                                 <  SRR4302543.6151372/94‑1 (MQ=60)
                            ACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAG                                                                 <  SRR4302543.7588738/94‑1 (MQ=60)
                             CGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGA                                                                <  SRR4302543.2415794/94‑1 (MQ=60)
                             CGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGA                                                                <  SRR4302543.5142240/94‑1 (MQ=60)
                             CGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGA                                                                >  SRR4302543.4299978/1‑94 (MQ=60)
                              GAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAA                                                               >  SRR4302543.5603492/1‑94 (MQ=60)
                              GAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAA                                                               <  SRR4302543.7643385/94‑1 (MQ=60)
                               AAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAAC                                                              <  SRR4302543.3761099/94‑1 (MQ=60)
                               AAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAAC                                                              <  SRR4302543.4119171/94‑1 (MQ=60)
                               AAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAAC                                                              >  SRR4302543.1876650/1‑94 (MQ=60)
                               AAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAAC                                                              >  SRR4302543.4266659/1‑94 (MQ=60)
                               AAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAAC                                                              >  SRR4302543.2188589/1‑94 (MQ=60)
                               AAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAAC                                                              <  SRR4302543.4246250/94‑1 (MQ=60)
                                AGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACT                                                             <  SRR4302543.3927582/94‑1 (MQ=60)
                                AGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACT                                                             >  SRR4302543.6562017/1‑94 (MQ=60)
                                AGAAGAGCAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATCAAGGTCATTTATATTAGCCCTTGCCTATTTCGGTTctctttataaagaact                                                             >  SRR4302543.6562016/1‑78 (MQ=60)
                                  AAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCT                                                           <  SRR4302543.3558495/94‑1 (MQ=60)
                                  AAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCT                                                           <  SRR4302543.7372925/94‑1 (MQ=60)
                                   AGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTA                                                          <  SRR4302543.1368521/94‑1 (MQ=60)
                                    GAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTAT                                                         <  SRR4302543.5855519/94‑1 (MQ=60)
                                    GAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTAT                                                         <  SRR4302543.7150013/94‑1 (MQ=60)
                                    GAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTAT                                                         <  SRR4302543.1581551/94‑1 (MQ=60)
                                    GAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTAT                                                         <  SRR4302543.7150014/94‑1 (MQ=60)
                                     AGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATA                                                        <  SRR4302543.802207/94‑1 (MQ=60)
                                     AGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATA                                                        >  SRR4302543.6889782/1‑94 (MQ=60)
                                     AGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTACCCCTTGGCTATTTTGGTTATATTAAATAAGAACTCTATA                                                        >  SRR4302543.6889781/1‑94 (MQ=60)
                                     AGTAGCTGATGGAGAAGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATA                                                        <  SRR4302543.4445360/94‑1 (MQ=60)
                                      GTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAA                                                       <  SRR4302543.4639915/94‑1 (MQ=60)
                                      GTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAA                                                       >  SRR4302543.7204179/1‑94 (MQ=60)
                                      GTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAA                                                       <  SRR4302543.6741620/94‑1 (MQ=60)
                                       TAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAA                                                      <  SRR4302543.4813593/94‑1 (MQ=60)
                                       TAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAA                                                      >  SRR4302543.3998010/1‑94 (MQ=60)
                                        AGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAT                                                     <  SRR4302543.6806588/94‑1 (MQ=60)
                                        AGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAT                                                     <  SRR4302543.2545371/94‑1 (MQ=60)
                                        AGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAT                                                     <  SRR4302543.2781086/94‑1 (MQ=60)
                                        AGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAT                                                     <  SRR4302543.5904688/94‑1 (MQ=60)
                                         GCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATT                                                    <  SRR4302543.3372130/94‑1 (MQ=60)
                                         GCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATT                                                    <  SRR4302543.7636878/94‑1 (MQ=60)
                                          CTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTT                                                   >  SRR4302543.783094/1‑94 (MQ=60)
                                          CTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTT                                                   <  SRR4302543.5154373/94‑1 (MQ=60)
                                           TGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTT                                                  >  SRR4302543.2690434/1‑94 (MQ=60)
                                           TGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTT                                                  >  SRR4302543.2690435/1‑94 (MQ=60)
                                            GATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTT                                                 >  SRR4302543.4542693/1‑94 (MQ=60)
                                            GATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTT                                                 >  SRR4302543.4542692/1‑94 (MQ=60)
                                             ATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTA                                                >  SRR4302543.1169081/1‑94 (MQ=60)
                                              TGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTAT                                               >  SRR4302543.2378382/1‑94 (MQ=60)
                                              TGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTAT                                               >  SRR4302543.4545182/1‑94 (MQ=60)
                                                GAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTC                                             <  SRR4302543.2554818/94‑1 (MQ=60)
                                                GAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTC                                             <  SRR4302543.1024927/94‑1 (MQ=60)
                                                 AGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCT                                            <  SRR4302543.689689/94‑1 (MQ=60)
                                                  GAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTT                                           <  SRR4302543.3848090/94‑1 (MQ=60)
                                                  GAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTT                                           <  SRR4302543.3848089/94‑1 (MQ=60)
                                                  GAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTT                                           <  SRR4302543.2191893/94‑1 (MQ=60)
                                                    GGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCG                                         <  SRR4302543.4476598/94‑1 (MQ=60)
                                                      GGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTT                                       <  SRR4302543.6057077/94‑1 (MQ=60)
                                                         GGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGC                                    <  SRR4302543.3042956/94‑1 (MQ=60)
                                                         GGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGC                                    >  SRR4302543.1257567/1‑94 (MQ=60)
                                                          GTGCTGCCTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCT                                   >  SRR4302543.3441604/1‑94 (MQ=60)
                                                                 CTCTCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACG                            >  SRR4302543.3006784/1‑94 (MQ=60)
                                                                    TCTCATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTCTAAATTTTTATTCTTCGTTGGCTCCATACGTGT                         >  SRR4302543.7682162/1‑94 (MQ=60)
                                                                       CATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTT                      >  SRR4302543.2974885/1‑94 (MQ=60)
                                                                        ATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTG                     <  SRR4302543.411606/94‑1 (MQ=60)
                                                                        ATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTG                     <  SRR4302543.6165580/94‑1 (MQ=60)
                                                                        ATTCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTG                     >  SRR4302543.4619223/1‑94 (MQ=60)
                                                                        ATCCAGGTCATTTATAGTAGCCCTTTGATATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTTTCTTG                     <  SRR4302543.411605/94‑1 (MQ=60)
                                                                          TCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCT                   >  SRR4302543.3532625/1‑94 (MQ=60)
                                                                          TCAGGTCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTT‑CTA                  >  SRR4302543.3686444/1‑94 (MQ=60)
                                                                               TCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATC              >  SRR4302543.3599417/1‑94 (MQ=60)
                                                                               TCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATC              <  SRR4302543.1779784/94‑1 (MQ=60)
                                                                               TCATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATC              >  SRR4302543.1001472/1‑94 (MQ=60)
                                                                                 ATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCAC            >  SRR4302543.4434927/1‑94 (MQ=60)
                                                                                 ATTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCAC            <  SRR4302543.3998075/94‑1 (MQ=60)
                                                                                  TTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACT           <  SRR4302543.384904/94‑1 (MQ=60)
                                                                                  TTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACT           <  SRR4302543.7372720/94‑1 (MQ=60)
                                                                                  TTTATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACT           <  SRR4302543.7977214/94‑1 (MQ=60)
                                                                                     ATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTT        >  SRR4302543.7946109/1‑94 (MQ=60)
                                                                                     ATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTT        >  SRR4302543.3594290/1‑94 (MQ=60)
                                                                                     ATATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGCCTCCATACGTGTCTTGCTACATCACTGTT        >  SRR4302543.5562567/1‑94 (MQ=60)
                                                                                      TATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTC       >  SRR4302543.2679836/1‑94 (MQ=60)
                                                                                      TATTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTC       >  SRR4302543.6902183/1‑94 (MQ=60)
                                                                                        TTAGCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCAG     <  SRR4302543.5887684/94‑1 (MQ=60)
                                                                                           GCCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCAGGAG  <  SRR4302543.1532518/94‑1 (MQ=60)
                                                                                            |                                                                                            
CCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCACTGTTCAGGAG  >  NC_000913/4075977‑4076161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑