Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,531,428	C→T	intergenic (‑76/‑1155)	yjhQ ← / → yjhR	hypothetical protein/putative frameshift suppressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,531,428	0	C	T	99.8%	1460.7 / ‑6.7	415	intergenic (‑76/‑1155)	yjhQ/yjhR	hypothetical protein/putative frameshift suppressor
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (208/205); total (210/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 425 total aligned reads displayed.

GTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGCCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAG  >  NC_000913/4531338‑4531517

gtggtgTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCa                                                                                         <  1:1977123/93‑1 (MQ=255)

 tggtgTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCAt                                                                                        <  2:70207/93‑1 (MQ=255)

 gggtgTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCAt                                                                                        <  1:3831950/92‑1 (MQ=255)

  ggtgTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCAtt                                                                                       <  1:5815668/93‑1 (MQ=255)

  ggtgTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCAtt                                                                                       >  2:2203452/1‑93 (MQ=255)

   gtgtACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTg                                                                                      >  1:1498190/1‑93 (MQ=255)

   gtgtACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTg                                                                                      >  2:2801905/1‑93 (MQ=255)

    tgtACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGa                                                                                     <  2:2513244/93‑1 (MQ=255)

    tgtACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGa                                                                                     <  2:5889582/93‑1 (MQ=255)

    ggtACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGa                                                                                     <  2:5979140/92‑1 (MQ=255)

      tACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACg                                                                                   >  1:4339304/1‑93 (MQ=255)

       aCTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACgg                                                                                  >  2:1234537/1‑93 (MQ=255)

        cTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGa                                                                                 >  2:3248856/1‑93 (MQ=255)

        cTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGa                                                                                 >  2:1806833/1‑93 (MQ=255)

        cTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGa                                                                                 >  2:4609827/1‑93 (MQ=255)

        cTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGa                                                                                 <  1:7596731/93‑1 (MQ=255)

         tGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGAc                                                                                >  1:5133880/1‑93 (MQ=255)

         tGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGAc                                                                                <  2:487853/93‑1 (MQ=255)

          gTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACt                                                                               <  2:3077512/93‑1 (MQ=255)

           tCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACtt                                                                              >  2:6878414/1‑93 (MQ=255)

           tCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACtt                                                                              >  2:6110246/1‑93 (MQ=255)

            cATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTg                                                                             <  2:4153766/93‑1 (MQ=255)

            cATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGGGGGGTTGATTGGATAGGGGTGGTCCGTGACGGGCTTg                                                                             >  1:6335508/1‑93 (MQ=255)

              tAGAAACTCTCCTTAGCGATTATCGCGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGAt                                                                           >  1:4126168/1‑93 (MQ=255)

              tAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGAt                                                                           <  1:561654/93‑1 (MQ=255)

               aGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATa                                                                          <  2:2591962/93‑1 (MQ=255)

               aGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATa                                                                          >  1:4823858/1‑93 (MQ=255)

                gAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATaa                                                                         >  1:2947298/1‑93 (MQ=255)

                  aaCTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGt                                                                       <  1:2202910/93‑1 (MQ=255)

                  aaCTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGt                                                                       <  2:1857034/93‑1 (MQ=255)

                   aCTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGtt                                                                      >  1:6414633/1‑93 (MQ=255)

                    ctctCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGttt                                                                     >  2:7497609/1‑93 (MQ=255)

                     tctcCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGtttt                                                                    >  2:794449/1‑93 (MQ=255)

                         cTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTgg                                                                >  1:6605265/1‑93 (MQ=255)

                         cTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTgg                                                                >  2:2959534/1‑93 (MQ=255)

                          ttAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGt                                                               >  1:6290165/1‑93 (MQ=255)

                          ttAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGt                                                               >  1:3992406/1‑93 (MQ=255)

                          ttAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGt                                                               >  1:7294060/1‑93 (MQ=255)

                             gCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGtttt                                                            <  1:5246380/93‑1 (MQ=255)

                             gCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGtttt                                                            <  2:5931226/93‑1 (MQ=255)

                             gCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGtttt                                                            <  2:7372307/93‑1 (MQ=255)

                               gATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTTACGGGCTTGATAAGTTTTTTGGTTTTGa                                                          >  2:1452596/1‑93 (MQ=255)

                               gATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGa                                                          >  1:5416801/1‑93 (MQ=255)

                               gATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGa                                                          <  1:5031445/93‑1 (MQ=255)

                                 ttATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGt                                                        <  2:5233819/93‑1 (MQ=255)

                                  tATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGtt                                                       <  2:1704677/93‑1 (MQ=255)

                                   aTCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTTATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGttt                                                      >  1:2676228/1‑93 (MQ=255)

                                   aTCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGttt                                                      >  1:3697052/1‑93 (MQ=255)

                                    tCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGa                                                          <  1:3579684/88‑1 (MQ=255)

                                     cGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGttttt                                                    <  2:4823857/93‑1 (MQ=255)

                                     cGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGttttt                                                    <  2:1327024/93‑1 (MQ=255)

                                      gAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGtttttt                                                   <  2:7819279/93‑1 (MQ=255)

                                      gAGCTGAGCGCGAACTTTATTCAGCTCGGCGGTGTTGATTCGATAGGGCTGGCCATTGACGGACTTGATAAATTTTTTGGTTTTGAGtttttt                                                   >  2:3686960/1‑93 (MQ=255)

                                        gCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGa                                                 >  2:5427873/1‑93 (MQ=255)

                                          tGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAg                                               <  1:1735506/93‑1 (MQ=255)

                                            aGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGAc                                             <  1:1183161/93‑1 (MQ=255)

                                            aGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGAc                                             <  2:6248906/93‑1 (MQ=255)

                                             gcgcGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGCAGatg                                            >  1:2839265/1‑91 (MQ=255)

                                              cgcgAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACgg                                           >  2:6766800/1‑93 (MQ=255)

                                              cgcgAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACgg                                           <  1:7914296/93‑1 (MQ=255)

                                                 gAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGa                                        <  2:290177/93‑1 (MQ=255)

                                                 gAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGa                                        <  2:7605170/93‑1 (MQ=255)

                                                  aaCTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAg                                       <  2:5599418/93‑1 (MQ=255)

                                                   aCTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGc                                      >  1:3192957/1‑93 (MQ=255)

                                                   aCTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGc                                      <  2:7726052/93‑1 (MQ=255)

                                                     tttATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGt                                    <  2:7650605/93‑1 (MQ=255)

                                                     tttATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGt                                    <  1:981699/93‑1 (MQ=255)

                                                      ttATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTg                                   >  1:5274069/1‑93 (MQ=255)

                                                        aTTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCa                                 <  2:2663697/93‑1 (MQ=255)

                                                            aGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCg                             <  2:1965904/93‑1 (MQ=255)

                                                            aGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCg                             >  2:6563875/1‑93 (MQ=255)

                                                             gCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCgg                            >  2:2150512/1‑93 (MQ=255)

                                                              cTCGGTGGTTGTGATTCGATAGGGCTGGTCATTGACGGACTAGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGt                           <  2:6437803/93‑1 (MQ=255)

                                                              cTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGt                           >  1:2627023/1‑93 (MQ=255)

                                                              cTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTGTTTTGAAGACGGCGAGCGTGCAGTCGGt                           >  1:1850396/1‑93 (MQ=255)

                                                               tCGGTGGTGTTGATTTGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTc                          <  1:6730167/93‑1 (MQ=255)

                                                                cGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCa                         <  2:7179170/93‑1 (MQ=255)

                                                                  gtggtgTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGc                       >  1:2400869/1‑93 (MQ=255)

                                                                    ggtgTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCaa                     <  1:837349/93‑1 (MQ=255)

                                                                     gtgtTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAAc                    >  2:5149466/1‑93 (MQ=255)

                                                                     gtgtTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAAc                    <  2:437711/93‑1 (MQ=255)

                                                                      tgtTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACa                   >  2:5076555/1‑93 (MQ=255)

                                                                       gtTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAg                  <  1:1349217/93‑1 (MQ=255)

                                                                        ttGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGc                 >  1:7773206/1‑93 (MQ=255)

                                                                          gATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGccc               <  1:7144559/93‑1 (MQ=255)

                                                                           aTTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCt              <  1:1021678/93‑1 (MQ=255)

                                                                            ttCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCtt             >  1:3754921/1‑93 (MQ=255)

                                                                            ttCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCtt             <  2:3296568/93‑1 (MQ=255)

                                                                            ttCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCtt             <  1:6034449/93‑1 (MQ=255)

                                                                            ttCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCtt             <  2:7915627/93‑1 (MQ=255)

                                                                             tCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTc            >  1:5964195/1‑93 (MQ=255)

                                                                              cGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTcg           >  2:3470771/1‑93 (MQ=255)

                                                                               taTAGGGCTGGTCATTTACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTcgc          <  1:2342112/92‑1 (MQ=255)

                                                                               gATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTcgc          <  1:4637967/93‑1 (MQ=255)

                                                                                aTAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTcgcg         >  2:7277547/1‑93 (MQ=255)

                                                                                  aGGGCTTGTCATTGACTGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGc       <  2:2103978/93‑1 (MQ=255)

                                                                                   gggCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCt      >  2:5707060/1‑93 (MQ=255)

                                                                                     gCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGt    >  1:3335636/1‑93 (MQ=255)

                                                                                       tGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAg  <  1:4034307/93‑1 (MQ=255)

                                                                                       tGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAg  >  1:4607540/1‑93 (MQ=255)

GTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGCCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAG  >  NC_000913/4531338‑4531517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 210 total aligned reads displayed.

GAAGTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGCCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGCAT  >  NC_000913/4531335‑4531520

GAAGTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGT                                                                                              <  SRR4302543.1642083/94‑1 (MQ=60)

GAAGTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGT                                                                                              >  SRR4302543.5551840/1‑94 (MQ=60)

 AAGTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTC                                                                                             >  SRR4302543.5576675/1‑94 (MQ=60)

  AGTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCA                                                                                            >  SRR4302543.3776668/1‑94 (MQ=60)

  AGTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCA                                                                                            >  SRR4302543.3924418/1‑94 (MQ=60)

  AGTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGACCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCA                                                                                            <  SRR4302543.797894/94‑1 (MQ=60)

   GTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCAT                                                                                           <  SRR4302543.1994849/94‑1 (MQ=60)

   GTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCAT                                                                                           >  SRR4302543.7799090/1‑94 (MQ=60)

    GGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATT                                                                                          <  SRR4302543.3865756/94‑1 (MQ=60)

     GGTGTGTTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGCTGGTGTTGATTCGATAGGGCTGGTCATTG                                                                                         <  SRR4302543.4046887/94‑1 (MQ=60)

     GGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTG                                                                                         <  SRR4302543.5868553/94‑1 (MQ=60)

     GGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTG                                                                                         >  SRR4302543.4505836/1‑94 (MQ=60)

     GGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTG                                                                                         >  SRR4302543.1511667/1‑94 (MQ=60)

     GGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTG                                                                                         >  SRR4302543.1567794/1‑94 (MQ=60)

     GGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTG                                                                                         <  SRR4302543.2924513/94‑1 (MQ=60)

     GGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTG                                                                                         <  SRR4302543.4046886/94‑1 (MQ=60)

      GTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGA                                                                                        <  SRR4302543.5794766/94‑1 (MQ=60)

      GTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGA                                                                                        <  SRR4302543.2676857/94‑1 (MQ=60)

       TGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGAC                                                                                       >  SRR4302543.5310139/1‑94 (MQ=60)

       TGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGAC                                                                                       >  SRR4302543.2014026/1‑94 (MQ=60)

       TGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGAC                                                                                       <  SRR4302543.1634752/94‑1 (MQ=60)

        GTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACG                                                                                      >  SRR4302543.4378666/1‑94 (MQ=60)

        GTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACG                                                                                      >  SRR4302543.6790914/1‑94 (MQ=60)

        GTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACG                                                                                      >  SRR4302543.5878904/1‑94 (MQ=60)

         TACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGG                                                                                     <  SRR4302543.6178448/94‑1 (MQ=60)

         TACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGG                                                                                     >  SRR4302543.4515284/1‑94 (MQ=60)

         TACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGG                                                                                     <  SRR4302543.155119/94‑1 (MQ=60)

         TACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGG                                                                                     <  SRR4302543.6375638/94‑1 (MQ=60)

         TACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGA‑TCGATAGGGCTGGGCATTGAaggg                                                                                    >  SRR4302543.1452805/1‑90 (MQ=60)

          ACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGA                                                                                    >  SRR4302543.5195575/1‑94 (MQ=60)

           CTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGAC                                                                                   <  SRR4302543.6255833/94‑1 (MQ=60)

           CTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGAC                                                                                   <  SRR4302543.3969276/94‑1 (MQ=60)

           CTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGAC                                                                                   >  SRR4302543.3095486/1‑94 (MQ=60)

           CTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGAC                                                                                   >  SRR4302543.3385806/1‑94 (MQ=60)

           CTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGAC                                                                                   <  SRR4302543.7668891/94‑1 (MQ=60)

            TGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTACTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACT                                                                                  <  SRR4302543.7869231/94‑1 (MQ=60)

             GTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTT                                                                                 <  SRR4302543.760379/94‑1 (MQ=60)

             GTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTT                                                                                 >  SRR4302543.3651147/1‑94 (MQ=60)

             GTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTT                                                                                 <  SRR4302543.1279124/94‑1 (MQ=60)

             GTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTT                                                                                 >  SRR4302543.87225/1‑94 (MQ=60)

              TCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTG                                                                                <  SRR4302543.5150804/94‑1 (MQ=60)

              TCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTG                                                                                >  SRR4302543.81310/1‑94 (MQ=60)

               CATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGA                                                                               >  SRR4302543.5613895/1‑94 (MQ=60)

                ATAGAACCTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGAT                                                                              <  SRR4302543.3764226/94‑1 (MQ=60)

                ATAGAAACTCTCCTTAGCGATTATCGCGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGAT                                                                              >  SRR4302543.4163375/1‑94 (MQ=60)

                ATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGAT                                                                              >  SRR4302543.3847382/1‑94 (MQ=60)

                ATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGAT                                                                              <  SRR4302543.6738526/94‑1 (MQ=60)

                 TAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATA                                                                             <  SRR4302543.566801/94‑1 (MQ=60)

                 TAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATA                                                                             >  SRR4302543.4867884/1‑94 (MQ=60)

                  AGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAA                                                                            >  SRR4302543.409886/1‑94 (MQ=60)

                  AGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAA                                                                            <  SRR4302543.5354088/94‑1 (MQ=60)

                  AGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAA                                                                            >  SRR4302543.4416462/1‑94 (MQ=60)

                  AGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAA                                                                            >  SRR4302543.2974059/1‑94 (MQ=60)

                  AAAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAA                                                                            <  SRR4302543.3034167/94‑1 (MQ=60)

                   GAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAG                                                                           <  SRR4302543.2312585/94‑1 (MQ=60)

                   GAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAG                                                                           <  SRR4302543.5446490/94‑1 (MQ=60)

                    AAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGT                                                                          >  SRR4302543.1828304/1‑94 (MQ=60)

                    AAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGT                                                                          >  SRR4302543.2637515/1‑94 (MQ=60)

                     AACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTT                                                                         <  SRR4302543.857029/94‑1 (MQ=60)

                     AACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTT                                                                         >  SRR4302543.6475055/1‑94 (MQ=60)

                     AACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTT                                                                         <  SRR4302543.2222735/94‑1 (MQ=60)

                        TCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTT                                                                      <  SRR4302543.5509261/94‑1 (MQ=60)

                        TCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTT                                                                      >  SRR4302543.1534493/1‑94 (MQ=60)

                         CTCCTTAGCGATTATCGAGCTGAGCGCGAACTTGATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTT                                                                     <  SRR4302543.7700193/94‑1 (MQ=60)

                          TCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTG                                                                    >  SRR4302543.3533080/1‑94 (MQ=60)

                          TCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTG                                                                    <  SRR4302543.3597545/94‑1 (MQ=60)

                           CCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGG                                                                   >  SRR4302543.6667518/1‑94 (MQ=60)

                           CCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGG                                                                   >  SRR4302543.6523967/1‑94 (MQ=60)

                           CCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGG                                                                   <  SRR4302543.1980285/94‑1 (MQ=60)

                           CCTTAGCGAGTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGG                                                                   <  SRR4302543.5682374/94‑1 (MQ=60)

                            CTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGT                                                                  >  SRR4302543.6349396/1‑94 (MQ=60)

                            CTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGT                                                                  >  SRR4302543.4027929/1‑94 (MQ=60)

                            CTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGT                                                                  >  SRR4302543.6296548/1‑94 (MQ=60)

                            CTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGT                                                                  >  SRR4302543.467630/1‑94 (MQ=60)

                            CTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGT                                                                  <  SRR4302543.3295129/94‑1 (MQ=60)

                            CTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGT                                                                  >  SRR4302543.7364448/1‑94 (MQ=60)

                             TTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTT                                                                 <  SRR4302543.2373041/94‑1 (MQ=60)

                                GCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTG                                                              <  SRR4302543.4660606/94‑1 (MQ=60)

                                GCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTG                                                              >  SRR4302543.3469317/1‑94 (MQ=60)

                                GCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTG                                                              <  SRR4302543.5293675/94‑1 (MQ=60)

                                GCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTG                                                              <  SRR4302543.4660604/94‑1 (MQ=60)

                                 CGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGA                                                             >  SRR4302543.6951350/1‑94 (MQ=60)

                                 CGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGA                                                             >  SRR4302543.2300017/1‑94 (MQ=60)

                                 CGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGA                                                             >  SRR4302543.5215048/1‑94 (MQ=60)

                                 CGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGA                                                             >  SRR4302543.5466075/1‑94 (MQ=60)

                                  ctcTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAG                                                            <  SRR4302543.3611308/91‑1 (MQ=60)

                                  GATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAG                                                            <  SRR4302543.5077216/94‑1 (MQ=60)

                                   ATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGT                                                           >  SRR4302543.1411435/1‑94 (MQ=60)

                                   ATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGT                                                           >  SRR4302543.5904658/1‑94 (MQ=60)

                                   ATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGT                                                           >  SRR4302543.8269/1‑94 (MQ=60)

                                    TTATCGAGCTGAGCGCGCACTATATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTT                                                          <  SRR4302543.5427881/94‑1 (MQ=60)

                                    TTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTT                                                          >  SRR4302543.3308631/1‑94 (MQ=60)

                                     TATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTTATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTT                                                         >  SRR4302543.2700492/1‑94 (MQ=60)

                                     TATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTT                                                         >  SRR4302543.3729555/1‑94 (MQ=60)

                                     TATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTT                                                         >  SRR4302543.2427397/1‑94 (MQ=60)

                                     TATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTT                                                         >  SRR4302543.3729556/1‑94 (MQ=60)

                                     TATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTT                                                         >  SRR4302543.6356955/1‑94 (MQ=60)

                                     TATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTT                                                         <  SRR4302543.5078582/94‑1 (MQ=60)

                                       TCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTT                                                       <  SRR4302543.6410822/94‑1 (MQ=60)

                                       TCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTT                                                       <  SRR4302543.6410827/94‑1 (MQ=60)

                                        CGAGCTGAGCGCGATCTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTT                                                      <  SRR4302543.7243914/94‑1 (MQ=60)

                                        CGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTT                                                      <  SRR4302543.2271615/94‑1 (MQ=60)

                                         GAGGTGAGGGGGAACTTTGTTCGGCCCGGTTGTGTTAATTCGATAGGGCTGGTCATTGACGAACTTGATAAGTTTTTTGGTTTTGAGTTTTTTG                                                     <  SRR4302543.4297250/94‑1 (MQ=50)

                                         GAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTG                                                     <  SRR4302543.4297249/94‑1 (MQ=60)

                                          AGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGA                                                    <  SRR4302543.2809394/94‑1 (MQ=60)

                                          AGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGA                                                    >  SRR4302543.3801004/1‑94 (MQ=60)

                                            CTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAG                                                  <  SRR4302543.3868630/94‑1 (MQ=60)

                                            CTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGAATTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAG                                                  >  SRR4302543.7685491/1‑94 (MQ=60)

                                             TGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGA                                                 >  SRR4302543.5196361/1‑94 (MQ=60)

                                             TGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGA                                                 >  SRR4302543.756375/1‑94 (MQ=60)

                                             TGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGA                                                 <  SRR4302543.1750976/94‑1 (MQ=60)

                                              GAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGAC                                                <  SRR4302543.5042518/94‑1 (MQ=60)

                                               AGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGCAGATG                                               >  SRR4302543.2865222/1‑94 (MQ=60)

                                               AGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACG                                               <  SRR4302543.1193849/94‑1 (MQ=60)

                                                 CGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGC                                             >  SRR4302543.4055608/1‑94 (MQ=60)

                                                 CGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGC                                             <  SRR4302543.7988644/94‑1 (MQ=60)

                                                 CGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGC                                             >  SRR4302543.6111348/1‑94 (MQ=60)

                                                  GCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCG                                            <  SRR4302543.2677096/94‑1 (MQ=60)

                                                  GCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCG                                            >  SRR4302543.6693654/1‑94 (MQ=60)

                                                   CGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGA                                           >  SRR4302543.6980176/1‑94 (MQ=60)

                                                    GAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAG                                          >  SRR4302543.2194161/1‑94 (MQ=60)

                                                     AACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGC                                         >  SRR4302543.3221743/1‑94 (MQ=60)

                                                      ACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCG                                        >  SRR4302543.6859291/1‑94 (MQ=60)

                                                      ACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCG                                        >  SRR4302543.3668593/1‑94 (MQ=60)

                                                      ACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCG                                        >  SRR4302543.4134182/1‑94 (MQ=60)

                                                      ACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCG                                        <  SRR4302543.5508843/94‑1 (MQ=60)

                                                       CTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGT                                       >  SRR4302543.81704/1‑94 (MQ=60)

                                                       CTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGT                                       >  SRR4302543.7289303/1‑94 (MQ=60)

                                                        TTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTG                                      >  SRR4302543.5321654/1‑94 (MQ=60)

                                                         TTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGTTTTTTAGTTTTTTTAAGACGGCGAGCGTGC                                     >  SRR4302543.6069102/1‑94 (MQ=60)

                                                          TATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCA                                    >  SRR4302543.4955341/1‑94 (MQ=60)

                                                          TATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCA                                    >  SRR4302543.4780511/1‑94 (MQ=60)

                                                           ATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAG                                   >  SRR4302543.2669278/1‑94 (MQ=60)

                                                            TTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGT                                  >  SRR4302543.1046831/1‑94 (MQ=60)

                                                              CAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCG                                >  SRR4302543.4351143/1‑94 (MQ=60)

                                                              CAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCG                                >  SRR4302543.7227846/1‑94 (MQ=60)

                                                              CAGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGACG                                >  SRR4302543.472189/1‑94 (MQ=60)

                                                               AGCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGG                               <  SRR4302543.7839433/94‑1 (MQ=60)

                                                                GCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGT                              >  SRR4302543.2650796/1‑94 (MQ=60)

                                                                GCTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTGTTTTGAAGACGGCGAGCGTGCAGTCGGT                              >  SRR4302543.1867040/1‑94 (MQ=60)

                                                                 CTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTC                             >  SRR4302543.6003512/1‑94 (MQ=60)

                                                                 CTCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTA                             >  SRR4302543.1948/1‑94 (MQ=60)

                                                                  TCGGTGGTGTTGATTTGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCA                            <  SRR4302543.6793745/94‑1 (MQ=60)

                                                                  TCGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCA                            >  SRR4302543.2811475/1‑94 (MQ=60)

                                                                   CGGTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAC                           >  SRR4302543.3734918/1‑94 (MQ=60)

                                                                    GGTGGTGTTGATTCGATAGGGCTGGTCATTGGCGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGC                          >  SRR4302543.2963574/1‑94 (MQ=60)

                                                                     GTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCA                         >  SRR4302543.2622072/1‑94 (MQ=60)

                                                                     GTGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCA                         <  SRR4302543.6029423/94‑1 (MQ=60)

                                                                      TGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAA                        <  SRR4302543.1790659/94‑1 (MQ=60)

                                                                      TGGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAA                        >  SRR4302543.2916260/1‑94 (MQ=60)

                                                                       GGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAATTTTTTTGGTTTTGAGTTTTTTGTAGACGTCGAGCGTGCAGTCGGTCAGCACC                       >  SRR4302543.5298095/1‑94 (MQ=60)

                                                                       GGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAAC                       >  SRR4302543.2768815/1‑94 (MQ=60)

                                                                       GGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAAC                       <  SRR4302543.2714145/94‑1 (MQ=60)

                                                                       GGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAAC                       >  SRR4302543.5298096/1‑94 (MQ=60)

                                                                       GGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAAC                       >  SRR4302543.140643/1‑94 (MQ=60)

                                                                       GGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAAC                       >  SRR4302543.6499807/1‑94 (MQ=60)

                                                                       GGTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAAC                       <  SRR4302543.844737/94‑1 (MQ=60)

                                                                        GTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACA                      <  SRR4302543.5159991/94‑1 (MQ=60)

                                                                        GTGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACA                      <  SRR4302543.5159992/94‑1 (MQ=60)

                                                                         TGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAG                     <  SRR4302543.6366669/94‑1 (MQ=60)

                                                                         TGTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAG                     >  SRR4302543.947194/1‑94 (MQ=60)

                                                                          GTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGC                    <  SRR4302543.1361285/94‑1 (MQ=60)

                                                                          GTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGC                    >  SRR4302543.7846524/1‑94 (MQ=60)

                                                                          GTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGC                    <  SRR4302543.1227983/94‑1 (MQ=60)

                                                                          GTTGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGC                    >  SRR4302543.1188303/1‑94 (MQ=60)

                                                                            TGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCC                  >  SRR4302543.1166613/1‑94 (MQ=60)

                                                                            TGATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCC                  <  SRR4302543.6719483/94‑1 (MQ=60)

                                                                             GATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCT                 >  SRR4302543.5663074/1‑94 (MQ=60)

                                                                             GATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCT                 <  SRR4302543.2286967/94‑1 (MQ=60)

                                                                             GATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCT                 <  SRR4302543.7213988/94‑1 (MQ=60)

                                                                              ATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGGAGTCGGTCAGCAACAGCCCTT                >  SRR4302543.7847658/1‑94 (MQ=60)

                                                                              ATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTT                >  SRR4302543.3787956/1‑94 (MQ=60)

                                                                              ATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTT                <  SRR4302543.1030717/94‑1 (MQ=60)

                                                                              ATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTT                >  SRR4302543.4922546/1‑94 (MQ=60)

                                                                              ATTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTT                >  SRR4302543.3902079/1‑94 (MQ=60)

                                                                               TTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTC               >  SRR4302543.6018808/1‑94 (MQ=60)

                                                                               TTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTC               <  SRR4302543.2369109/94‑1 (MQ=60)

                                                                               TTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTC               <  SRR4302543.4293349/94‑1 (MQ=60)

                                                                               TTCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTC               <  SRR4302543.6091168/94‑1 (MQ=60)

                                                                                TCGATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCG              <  SRR4302543.4114146/94‑1 (MQ=60)

                                                                                  TATAGGGCTGGTCATTTACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCG            <  SRR4302543.2363359/94‑1 (MQ=60)

                                                                                  GATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCG            <  SRR4302543.4680148/94‑1 (MQ=60)

                                                                                  GATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCG            <  SRR4302543.2363360/94‑1 (MQ=60)

                                                                                  GATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCG            <  SRR4302543.1353823/94‑1 (MQ=60)

                                                                                  GATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCG            >  SRR4302543.7917492/1‑94 (MQ=60)

                                                                                  AATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCG            <  SRR4302543.6549184/94‑1 (MQ=60)

                                                                                   ATAGGGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGG           >  SRR4302543.1964752/1‑94 (MQ=60)

                                                                                       GGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGT       >  SRR4302543.3365696/1‑94 (MQ=60)

                                                                                       GGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGT       <  SRR4302543.4837796/94‑1 (MQ=60)

                                                                                       GGCTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGT       <  SRR4302543.2073613/94‑1 (MQ=60)

                                                                                         CTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAG     <  SRR4302543.608410/94‑1 (MQ=60)

                                                                                         CTGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAG     >  SRR4302543.4649390/1‑94 (MQ=60)

                                                                                          TGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGGCCTTCGCGGCTGTAGC    >  SRR4302543.2266377/1‑94 (MQ=60)

                                                                                          TGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGC    >  SRR4302543.6250205/1‑94 (MQ=60)

                                                                                          TGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGC    <  SRR4302543.4070389/94‑1 (MQ=60)

                                                                                          TGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGC    >  SRR4302543.2266379/1‑94 (MQ=60)

                                                                                          TGGTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGC    <  SRR4302543.3312388/94‑1 (MQ=60)

                                                                                            GTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGCAT  >  SRR4302543.2676851/1‑94 (MQ=60)

                                                                                            GTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGCAT  <  SRR4302543.4867640/94‑1 (MQ=60)

                                                                                            GTCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGCAT  <  SRR4302543.6671869/94‑1 (MQ=60)

GAAGTGGTGTACTGTCATAGAAACTCTCCTTAGCGATTATCGAGCTGAGCGCGAACTTTATTCAGCTCGGTGGTGTTGATTCGATAGGGCTGGCCATTGACGGACTTGATAAGTTTTTTGGTTTTGAGTTTTTTGAAGACGGCGAGCGTGCAGTCGGTCAGCAACAGCCCTTCGCGGCTGTAGCAT  >  NC_000913/4531335‑4531520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`