Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	981,566	C→T	R433C (CGC→TGC)	ycbB →	putative amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	981,566	0	C	T	99.7%	1047.7 / NA	295	R433C (CGC→TGC)	ycbB	putative amidase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (141/153); minor base G (1/0); total (142/153)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

GGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCACGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAA  >  NC_000913/981476‑981655

ggtcgCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGc                                                                                         >  1:7040480/1‑93 (MQ=255)

 gtcgCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCa                                                                                        <  1:5350563/93‑1 (MQ=255)

  tcgCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCaa                                                                                       >  2:876400/1‑93 (MQ=255)

      cccGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGat                                                                                   >  1:1293212/1‑93 (MQ=255)

       ccGATCGCAAAACGCCGATGATGAGCAGTGGCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGata                                                                                  >  1:2691545/1‑93 (MQ=255)

       ccGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGata                                                                                  <  1:4286981/93‑1 (MQ=255)

       ccGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGata                                                                                  >  1:670958/1‑93 (MQ=255)

       ccGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGata                                                                                  >  1:6646426/1‑93 (MQ=255)

        cGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGatat                                                                                 <  2:604284/93‑1 (MQ=255)

         gATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATAtt                                                                                >  2:6543928/1‑93 (MQ=255)

          aTCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGCCATGCAAAGATATTc                                                                               >  1:4538704/1‑93 (MQ=255)

          aTCGCAAAACGCCGATGATGAGCAGGGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTCGCATGCAAAGatatac                                                                               >  2:1575262/1‑91 (MQ=255)

            cGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTCCCTCCAACTCTGGCATGCAAAGATATTCTg                                                                             <  2:6714399/93‑1 (MQ=255)

             gCAAAACGCCGATGATTAGCAGTGCCCTTAACAACGTAGTGGTAAT‑CCCTTCGTGGAACGTACCTCCAACTCTGGCATGCTAAGATAATCTGc                                                                            >  2:7836448/1‑93 (MQ=255)

               aaaaCGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCa                                                                          >  1:41207/1‑93 (MQ=255)

                aaaCGCCGATGATGAGCAGTGCCCTTAACAAGGTAGGGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaa                                                                         <  1:566964/93‑1 (MQ=255)

                aaaCGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaa                                                                         >  2:6265299/1‑93 (MQ=255)

                aaaCGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaa                                                                         >  1:7440692/1‑93 (MQ=255)

                  aCGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaaaa                                                                       >  1:8777346/1‑93 (MQ=255)

                  aCGCCGATGAGGGAGAGTGCCCTTAACAACGTAGTGGTAAACCCCGCCG‑GGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaaaa                                                                       <  1:5755052/93‑1 (MQ=255)

                   cGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAg                                                                      <  2:7952243/93‑1 (MQ=255)

                    gCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGt                                                                     <  1:8212275/93‑1 (MQ=255)

                    gCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGt                                                                     >  1:5202091/1‑93 (MQ=255)

                    gCCGATAATGGGCAGTGCCCTAAACAACGAAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGt                                                                     <  2:7008686/93‑1 (MQ=255)

                      cGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTgc                                                                   >  1:2250415/1‑93 (MQ=255)

                        atgatgAGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTgcgc                                                                 <  2:260704/93‑1 (MQ=255)

                        atgatgAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTgcgc                                                                 <  2:4965911/93‑1 (MQ=255)

                          gatgaGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCaa                                                               >  2:2729172/1‑93 (MQ=255)

                           atgaGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAAc                                                              <  1:5885964/93‑1 (MQ=255)

                             gaGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGa                                                            >  1:537266/1‑93 (MQ=255)

                             gaGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGa                                                            >  1:1859746/1‑93 (MQ=255)

                             gaGCAGTCCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGa                                                            <  2:4015705/93‑1 (MQ=255)

                              atcggTGCCCTTAACAACGTAGTGGAAAG‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGAt                                                           <  1:7595343/89‑1 (MQ=255)

                              aGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGAt                                                           <  1:6844837/93‑1 (MQ=255)

                               gCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATc                                                          <  2:1909708/93‑1 (MQ=255)

                               gCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATc                                                          <  2:7036442/93‑1 (MQ=255)

                                cggTGCCCTTAACATCTTGGTGGAAAC‑CCCGCCGTGGAACGTACCTCCAACCCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATcc                                                         <  2:4779264/91‑1 (MQ=255)

                                 aGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATACg                                                        <  1:8365424/93‑1 (MQ=255)

                                  gTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCgg                                                       <  1:4346193/93‑1 (MQ=255)

                                  gTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCgg                                                       >  1:1680689/1‑93 (MQ=255)

                                   tGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCggg                                                      <  1:4470952/93‑1 (MQ=255)

                                    ccccTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGa                                                     <  2:2143006/92‑1 (MQ=255)

                                     cccTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGat                                                    >  2:2306753/1‑93 (MQ=255)

                                     cccTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGat                                                    <  2:1320430/93‑1 (MQ=255)

                                       cTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGatat                                                  <  2:4318734/93‑1 (MQ=255)

                                        ttAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATAtc                                                 >  1:2367097/1‑93 (MQ=255)

                                        ttAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATAtc                                                 <  2:7358100/93‑1 (MQ=255)

                                         tAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATAtct                                                <  1:3848832/93‑1 (MQ=255)

                                           acaccGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCg                                              <  2:5070571/93‑1 (MQ=255)

                                           acaacGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCg                                              <  1:714984/93‑1 (MQ=255)

                                             agcGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGaa                                            <  2:6843800/91‑1 (MQ=255)

                                             aacTTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGaa                                            <  2:412698/93‑1 (MQ=255)

                                              acGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGaaa                                           >  1:2580781/1‑93 (MQ=255)

                                                gTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGc                                         >  2:1280742/1‑93 (MQ=255)

                                                gTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGc                                         <  1:3234335/93‑1 (MQ=255)

                                                gTAGTGGAAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGc                                         <  1:7551149/93‑1 (MQ=255)

                                                 tAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGcc                                        >  1:2112854/1‑93 (MQ=255)

                                                 tAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGcc                                        <  2:8709635/93‑1 (MQ=255)

                                                  aGTGGTAAA‑CCCGCCGTGGAACGTGCCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCa                                       <  1:7780253/93‑1 (MQ=255)

                                                  aGTGGTAAA‑CCCGCCGTGGAACGTACCTGCAACTCTGGCATGCAAAGATATTCTGCCAAAAGGGCGCAACGATCCGGGATATCTCGAAAGCCa                                       >  1:858627/1‑93 (MQ=255)

                                                  aGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGcaa                                       >  1:2853413/1‑91 (MQ=255)

                                                   gTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCAt                                      >  1:7883100/1‑93 (MQ=255)

                                                     ggTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATgg                                    >  2:7574996/1‑93 (MQ=255)

                                                     ggTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATgg                                    <  2:3637455/93‑1 (MQ=255)

                                                      gTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGc                                   >  1:1071227/1‑93 (MQ=255)

                                                      gTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGc                                   <  2:7102692/93‑1 (MQ=255)

                                                       gaaa‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCt                                  <  1:1822716/92‑1 (MQ=255)

                                                         aa‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCtat                                >  1:5092763/1‑93 (MQ=255)

                                                          a‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCtata                               <  1:6159783/93‑1 (MQ=255)

                                                               gccgTGGAGCGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATATCGAAAGCCATGGCTATACGGt                           <  2:8323803/93‑1 (MQ=255)

                                                               gccgTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGt                           >  2:6167981/1‑93 (MQ=255)

                                                                ccgTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTg                          >  2:4721605/1‑93 (MQ=255)

                                                                 cgTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTTCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGa                         <  1:7371958/93‑1 (MQ=255)

                                                                 cgTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGa                         <  1:978622/93‑1 (MQ=255)

                                                                 cgTGAAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGa                         <  1:119610/93‑1 (MQ=255)

                                                                  gTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAt                        >  1:472543/1‑93 (MQ=255)

                                                                  gTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAt                        >  2:3853380/1‑93 (MQ=255)

                                                                    ggAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgc                      <  2:5420300/93‑1 (MQ=255)

                                                                    ggAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgc                      <  2:3760926/93‑1 (MQ=255)

                                                                     gAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgcg                     >  2:4820368/1‑93 (MQ=255)

                                                                     gAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgcg                     >  2:1240756/1‑93 (MQ=255)

                                                                      aaCGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgcgc                    >  2:240928/1‑93 (MQ=255)

                                                                       aCGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGcgcg                   >  2:2900724/1‑93 (MQ=255)

                                                                       aCGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGcgcg                   >  2:1127566/1‑93 (MQ=255)

                                                                        cGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCgg                  >  2:8498959/1‑93 (MQ=255)

                                                                         gTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGc                 <  2:3063501/93‑1 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                <  2:5833895/93‑1 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                >  1:4058093/1‑93 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                >  2:3124782/1‑93 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                >  2:6996336/1‑93 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                >  1:3059836/1‑93 (MQ=255)

                                                                                 aaCTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcag         <  2:5299148/93‑1 (MQ=255)

                                                                                  aCTCTGGCATGCAAAGATATTCCGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcagc        >  2:7784294/1‑93 (MQ=255)

                                                                                   ctctGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcagca       <  1:6321701/93‑1 (MQ=255)

                                                                                   ctctGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcagca       <  2:4515610/93‑1 (MQ=255)

                                                                                    tctGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcagcag      >  2:5583480/1‑93 (MQ=255)

                                                                                     ctGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCaga     <  1:1468822/93‑1 (MQ=255)

                                                                                     caggCATGCAAAGATATTCTGCCAAAAGTCCGCATCGATCCGGGATATCACGAAAGCCATGGCTATACTGGGATGCGCGGCTGGAACAGCaga     <  2:7686420/91‑1 (MQ=255)

                                                                                      tGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCagag    >  1:65646/1‑93 (MQ=255)

                                                                                       ggCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAgaga   <  2:334319/93‑1 (MQ=255)

GGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCACGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAA  >  NC_000913/981476‑981655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCACGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAGCG  >  NC_000913/981474‑981658

TCGGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATG                                                                                             <  SRR4302541.5300183/94‑1 (MQ=60)

 CGGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGC                                                                                            >  SRR4302541.7115329/1‑94 (MQ=60)

  GGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCA                                                                                           <  SRR4302541.6681529/94‑1 (MQ=60)

   GTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAA                                                                                          <  SRR4302541.5405552/94‑1 (MQ=60)

    TCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAA                                                                                         >  SRR4302541.3025564/1‑94 (MQ=60)

       CCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGAT                                                                                      >  SRR4302541.5895871/1‑94 (MQ=60)

       CCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGAT                                                                                      >  SRR4302541.1305976/1‑94 (MQ=60)

       CCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGAT                                                                                      >  SRR4302541.2631855/1‑94 (MQ=60)

        CCCGATCGCAAAACGCCGATGATGAGCAGTGGCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATA                                                                                     >  SRR4302541.2718281/1‑94 (MQ=60)

        CCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATA                                                                                     >  SRR4302541.6714603/1‑94 (MQ=60)

        CCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATA                                                                                     <  SRR4302541.8748005/94‑1 (MQ=60)

        CCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATA                                                                                     >  SRR4302541.677289/1‑94 (MQ=60)

         CCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATAT                                                                                    <  SRR4302541.213550/94‑1 (MQ=60)

         CCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATAT                                                                                    <  SRR4302541.225811/94‑1 (MQ=60)

         CCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATAT                                                                                    <  SRR4302541.4689346/94‑1 (MQ=60)

         CCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATAT                                                                                    <  SRR4302541.4329809/94‑1 (MQ=60)

          CGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATT                                                                                   >  SRR4302541.7517935/1‑94 (MQ=60)

          CGAGCGCAAAACGCAGATGCTGAGCAGTGCCCTTACCAACGTAGTGGTAAG‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATT                                                                                   <  SRR4302541.5869593/94‑1 (MQ=60)

           GATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTC                                                                                  <  SRR4302541.1851169/94‑1 (MQ=60)

           GATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTC                                                                                  >  SRR4302541.67543/1‑94 (MQ=60)

           GATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGCCATGCAAAGATATTC                                                                                  >  SRR4302541.4584575/1‑94 (MQ=60)

           CATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTC                                                                                  <  SRR4302541.1851167/94‑1 (MQ=60)

            ATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCT                                                                                 <  SRR4302541.5733311/94‑1 (MQ=60)

             TCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTG                                                                                >  SRR4302541.4230733/1‑94 (MQ=60)

              CGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGC                                                                               >  SRR4302541.2362063/1‑94 (MQ=60)

                CAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCA                                                                             >  SRR4302541.41518/1‑94 (MQ=60)

                 AAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAA                                                                            >  SRR4302541.7520207/1‑94 (MQ=60)

                 AAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACATCCAACTCTGGCATGCAAAGATATTCTGCCAA                                                                            >  SRR4302541.447361/1‑94 (MQ=60)

                  AACCGCCGATGATGAGCAGTGCCTTAAACAAGGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAA                                                                           <  SRR4302541.6386533/94‑1 (MQ=60)

                  AAACGCCGATGATGAGCAGTGCCCTTAACAAGGTAGGGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAA                                                                           <  SRR4302541.572292/94‑1 (MQ=60)

                  AAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAA                                                                           >  SRR4302541.6761131/1‑94 (MQ=60)

                   AACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAA                                                                          >  SRR4302541.8871150/1‑94 (MQ=60)

                   AACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAA                                                                          >  SRR4302541.530893/1‑94 (MQ=60)

                    acgccgatgagggagAGTGCCCTTAACAACGTAGTGGTAAACCCCGCCG‑GGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAG                                                                         <  SRR4302541.5813637/79‑1 (MQ=60)

                     CGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGT                                                                        >  SRR4302541.5255665/1‑94 (MQ=60)

                     CGCCGATGATGAGCAGTCCCCTTAACACCGTAGTGTTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGT                                                                        <  SRR4302541.454111/94‑1 (MQ=60)

                      GCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTG                                                                       <  SRR4302541.8301866/94‑1 (MQ=60)

                      CCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTG                                                                       <  SRR4302541.5688772/94‑1 (MQ=60)

                       CCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGC                                                                      >  SRR4302541.2272165/1‑94 (MQ=60)

                        CGATGATGAGGAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCG                                                                     <  SRR4302541.7606304/94‑1 (MQ=60)

                        CGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCG                                                                     <  SRR4302541.5393615/94‑1 (MQ=60)

                        CGATGATGAGCAGTGCCCTAAACAACGTAGGGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCG                                                                     <  SRR4302541.5065057/94‑1 (MQ=60)

                         GATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGC                                                                    >  SRR4302541.3264974/1‑94 (MQ=60)

                          ATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCA                                                                   >  SRR4302541.2520320/1‑94 (MQ=60)

                           TGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAA                                                                  >  SRR4302541.4836107/1‑94 (MQ=60)

                            GATGAGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAAC                                                                 <  SRR4302541.1752974/94‑1 (MQ=60)

                            GATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAAC                                                                 >  SRR4302541.1523475/1‑94 (MQ=60)

                             ATNANCNGNGCCCTTAACAACNTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACG                                                                <  SRR4302541.1146872/94‑1 (MQ=60)

                             ATGAGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACG                                                                <  SRR4302541.5945862/94‑1 (MQ=60)

                             ATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACG                                                                >  SRR4302541.119719/1‑94 (MQ=60)

                             ATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTCGAACGTACCTCCACCTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACG                                                                >  SRR4302541.5419523/1‑94 (MQ=60)

                              TGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGA                                                               >  SRR4302541.1877688/1‑94 (MQ=60)

                              TGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGA                                                               >  SRR4302541.542301/1‑94 (MQ=60)

                                atcgGTGCCCTTAACAACGTAGTGGAAAG‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATC                                                             <  SRR4302541.7676486/90‑1 (MQ=60)

                                AGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATC                                                             <  SRR4302541.6918023/94‑1 (MQ=60)

                                AGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCcagagtgggcaccggtc                                                             >  SRR4302541.5739628/1‑77 (MQ=60)

                                   AGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGG                                                          >  SRR4302541.1697219/1‑94 (MQ=60)

                                   AGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGG                                                          >  SRR4302541.602248/1‑94 (MQ=60)

                                   AGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATACGG                                                          <  SRR4302541.8456129/94‑1 (MQ=60)

                                    GTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGG                                                         <  SRR4302541.5224103/94‑1 (MQ=60)

                                    GTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGG                                                         >  SRR4302541.7666158/1‑94 (MQ=60)

                                    GTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGG                                                         >  SRR4302541.5410497/1‑94 (MQ=60)

                                    GTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGG                                                         <  SRR4302541.4389585/94‑1 (MQ=60)

                                     TGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGA                                                        <  SRR4302541.4100893/94‑1 (MQ=60)

                                     TGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGA                                                        >  SRR4302541.5186810/1‑94 (MQ=60)

                                     TGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGA                                                        <  SRR4302541.4515994/94‑1 (MQ=60)

                                     TGCCCTAAACAACGTGGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGA                                                        <  SRR4302541.8209082/94‑1 (MQ=60)

                                     TCCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGA                                                        <  SRR4302541.8577571/94‑1 (MQ=60)

                                      GCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGAT                                                       <  SRR4302541.2831216/94‑1 (MQ=60)

                                      GCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGAT                                                       <  SRR4302541.3777409/94‑1 (MQ=60)

                                      GCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGAT                                                       >  SRR4302541.1348067/1‑94 (MQ=60)

                                      GCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCCGCCAAAAGTGCGCAACGATCCGGGAT                                                       <  SRR4302541.4284454/94‑1 (MQ=60)

                                        cgtgAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATAT                                                     <  SRR4302541.4512572/90‑1 (MQ=60)

                                         CTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATC                                                    >  SRR4302541.5692349/1‑94 (MQ=60)

                                         CTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATC                                                    >  SRR4302541.2390190/1‑94 (MQ=60)

                                          TTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCT                                                   <  SRR4302541.776469/94‑1 (MQ=60)

                                           TAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTC                                                  >  SRR4302541.2040946/1‑94 (MQ=60)

                                           TAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTC                                                  <  SRR4302541.3887579/94‑1 (MQ=60)

                                             ACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGA                                                <  SRR4302541.721730/94‑1 (MQ=60)

                                              AAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAA                                               <  SRR4302541.397094/94‑1 (MQ=60)

                                               AACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAA                                              >  SRR4302541.2606649/1‑94 (MQ=60)

                                                ACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAG                                             <  SRR4302541.2504912/94‑1 (MQ=60)

                                                ACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAG                                             <  SRR4302541.4331203/94‑1 (MQ=60)

                                                ACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAG                                             <  SRR4302541.4331204/94‑1 (MQ=60)

                                                 CGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGC                                            >  SRR4302541.1228359/1‑94 (MQ=60)

                                                  GTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCC                                           <  SRR4302541.6708332/94‑1 (MQ=60)

                                                  GTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCC                                           >  SRR4302541.2133319/1‑94 (MQ=60)

                                                  GTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCC                                           <  SRR4302541.3266994/94‑1 (MQ=60)

                                                  GTAGTGGAAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCC                                           <  SRR4302541.7631842/94‑1 (MQ=60)

                                                   TAGTGGTAAA‑CCCGCCGTGGAACGTACCTGCAACTCTGGCATGCAAAGATATTCTGCCAAAAGGGCGCAACGATCCGGGATATCTCGAAAGCCA                                          >  SRR4302541.866431/1‑94 (MQ=60)

                                                   TAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCAA                                          >  SRR4302541.2882103/1‑94 (MQ=60)

                                                    AGTGGTAAA‑CCCGCCGTGGAAGGTACCTCTAACTCTGGCATGCAAAGATATTCTGCCAGAAGTGCGCAACGATCCGGGATATCTCGACAGCCAT                                         >  SRR4302541.7969751/1‑94 (MQ=60)

                                                    AGTGGTAAA‑CCCGCCGTGGAACGTGCCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCAT                                         <  SRR4302541.7863020/94‑1 (MQ=60)

                                                    AGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCAT                                         >  SRR4302541.7969752/1‑94 (MQ=60)

                                                     GTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATG                                        <  SRR4302541.742073/94‑1 (MQ=60)

                                                     GTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATG                                        <  SRR4302541.3427941/94‑1 (MQ=60)

                                                      TGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGG                                       >  SRR4302541.7033690/1‑94 (MQ=60)

                                                       GGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGC                                      <  SRR4302541.3056537/94‑1 (MQ=60)

                                                       GGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGC                                      >  SRR4302541.1081195/1‑94 (MQ=60)

                                                       GGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGC                                      <  SRR4302541.1680848/94‑1 (MQ=60)

                                                       GGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGC                                      >  SRR4302541.7042486/1‑94 (MQ=60)

                                                       GGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGC                                      >  SRR4302541.7275095/1‑94 (MQ=60)

                                                         TAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTA                                    <  SRR4302541.6356176/94‑1 (MQ=60)

                                                         GAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTA                                    <  SRR4302541.1840412/94‑1 (MQ=60)

                                                          AAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTAT                                   >  SRR4302541.5145069/1‑94 (MQ=60)

                                                          AAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTAT                                   >  SRR4302541.7678607/1‑94 (MQ=60)

                                                          AAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTAT                                   >  SRR4302541.7678609/1‑94 (MQ=60)

                                                            A‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATAC                                 <  SRR4302541.6222733/94‑1 (MQ=60)

                                                            A‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATACGGGATATCTCGAAAGCCATGGCTATAC                                 <  SRR4302541.3947902/94‑1 (MQ=60)

                                                               CCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGG                               >  SRR4302541.8438911/1‑94 (MQ=60)

                                                                 GCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTG                             >  SRR4302541.3153461/1‑94 (MQ=60)

                                                                  CCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGA                            >  SRR4302541.6352798/1‑94 (MQ=60)

                                                                   CGTTGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAT                           <  SRR4302541.6857058/94‑1 (MQ=60)

                                                                   CGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTTCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAT                           <  SRR4302541.7450747/94‑1 (MQ=60)

                                                                   CGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAT                           <  SRR4302541.987785/94‑1 (MQ=60)

                                                                   CGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAT                           <  SRR4302541.7450748/94‑1 (MQ=60)

                                                                   CGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAT                           >  SRR4302541.477033/1‑94 (MQ=60)

                                                                   CGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAT                           >  SRR4302541.6902983/1‑94 (MQ=60)

                                                                   CGTGAAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAT                           <  SRR4302541.120562/94‑1 (MQ=60)

                                                                    GTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATG                          <  SRR4302541.8525456/94‑1 (MQ=60)

                                                                     TGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGC                         <  SRR4302541.8341933/94‑1 (MQ=60)

                                                                     TGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGC                         <  SRR4302541.4565477/94‑1 (MQ=60)

                                                                       GAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGC                       <  SRR4302541.4760257/94‑1 (MQ=60)

                                                                        AACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCG                      <  SRR4302541.1393880/94‑1 (MQ=60)

                                                                         ACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGG                     <  SRR4302541.2375427/94‑1 (MQ=60)

                                                                         ACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGG                     <  SRR4302541.7931471/94‑1 (MQ=60)

                                                                           GTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCT                   >  SRR4302541.8278312/1‑94 (MQ=60)

                                                                           GTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCT                   >  SRR4302541.4098139/1‑94 (MQ=60)

                                                                           GTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCT                   >  SRR4302541.3090834/1‑94 (MQ=60)

                                                                            TACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTG                  >  SRR4302541.7529558/1‑94 (MQ=60)

                                                                              CCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGA                <  SRR4302541.4139084/94‑1 (MQ=60)

                                                                                 CCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACA             >  SRR4302541.2631885/1‑94 (MQ=60)

                                                                                  CAACTCTGGCATGCAAAGATATTCTGCCAAAAGGGCGCAACGATCCGGGACATCTCGAAAGCCATGGCTATACGGTGATCCGCGGCTGGAACAC            >  SRR4302541.7147632/1‑94 (MQ=60)

                                                                                   AACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGA           >  SRR4302541.1768566/1‑94 (MQ=60)

                                                                                    ACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCA          >  SRR4302541.1107975/1‑94 (MQ=60)

                                                                                    ACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCA          >  SRR4302541.7127639/1‑94 (MQ=60)

                                                                                    ACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCA          <  SRR4302541.2068690/94‑1 (MQ=60)

                                                                                     CTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTCTACGGTGATGCGCGGCTGGAACAGCAG         >  SRR4302541.98067/1‑94 (MQ=60)

                                                                                     CTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAG         <  SRR4302541.6386333/94‑1 (MQ=60)

                                                                                       CTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAG       <  SRR4302541.1483224/94‑1 (MQ=60)

                                                                                       CTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAG       <  SRR4302541.92743/94‑1 (MQ=60)

                                                                                       CTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAG       >  SRR4302541.1274616/1‑94 (MQ=60)

                                                                                       CTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAG       >  SRR4302541.66186/1‑94 (MQ=60)

                                                                                       CTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAG       >  SRR4302541.2875068/1‑94 (MQ=60)

                                                                                        TGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGA      <  SRR4302541.869579/94‑1 (MQ=60)

                                                                                        TGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGA      >  SRR4302541.5975813/1‑94 (MQ=60)

                                                                                          GCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAG    <  SRR4302541.6609377/94‑1 (MQ=60)

                                                                                           CATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAGC   >  SRR4302541.7179092/1‑94 (MQ=60)

                                                                                           CATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAGC   >  SRR4302541.2166161/1‑94 (MQ=60)

                                                                                            ATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAGCG  <  SRR4302541.1149402/94‑1 (MQ=60)

                                                                                            ATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAGCG  <  SRR4302541.2586310/94‑1 (MQ=60)

                                                                                            ATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAGCG  >  SRR4302541.4078607/1‑94 (MQ=60)

                                                                                            ATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAGCG  >  SRR4302541.3681496/1‑94 (MQ=60)

TCGGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCACGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAAGCG  >  NC_000913/981474‑981658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`