Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,672,529 T→C V198A (GTG→GCG)  b1601 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,672,5290TC98.8% 863.9 / ‑1.2 258V198A (GTG→GCG) b1601putative transport protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (119/136);  total (120/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 265 total aligned reads displayed.

TGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAT  >  NC_000913/1672439‑1672618
                                                                                          |                                                                                         
tGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGgcgc                                                                                         >  1:4564414/1‑93 (MQ=255)
 gCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCt                                                                                        >  2:3053770/1‑93 (MQ=255)
   ccGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTTAACGTGCGATTGACAGTGTTTCTCATTATCTTGCGCTGa                                                                                      <  1:3739524/93‑1 (MQ=255)
   ccGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGa                                                                                      <  2:3839938/93‑1 (MQ=255)
    cGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGTGATTGACAGTGTTTCTCATTATCTGGCGCTGaa                                                                                     >  1:8504852/1‑93 (MQ=255)
     ggAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGaaa                                                                                    >  2:7287016/1‑93 (MQ=255)
     ggAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGaaa                                                                                    >  1:145354/1‑93 (MQ=255)
      gAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGaaaa                                                                                   <  2:3267504/93‑1 (MQ=255)
      gAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGaaaa                                                                                   <  1:1030002/93‑1 (MQ=255)
       aaaaTTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAAc                                                                                  >  1:2961156/1‑93 (MQ=255)
       aaaaTTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAAc                                                                                  >  2:4460508/1‑93 (MQ=255)
        aaaTTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACa                                                                                 <  1:8436673/93‑1 (MQ=255)
        aaaTTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACa                                                                                 >  2:634769/1‑93 (MQ=255)
          aTTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGc                                                                               <  1:4298941/93‑1 (MQ=255)
           tttCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGcc                                                                              >  2:4056514/1‑93 (MQ=255)
            ttCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCa                                                                             >  2:7828853/1‑93 (MQ=255)
               aGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAGAACAGCCATCa                                                                          <  2:5058597/93‑1 (MQ=255)
               aGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCa                                                                          <  2:6283164/93‑1 (MQ=255)
                gCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAg                                                                         >  1:6747934/1‑93 (MQ=255)
                gCAAATGATGGCGCCTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAg                                                                         <  2:4941634/93‑1 (MQ=255)
                 cAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAATACAGCCATGAga                                                                        >  2:3305699/1‑92 (MQ=255)
                 cAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGc                                                                        <  1:5129305/93‑1 (MQ=255)
                  aaaTGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGca                                                                       <  1:4149353/93‑1 (MQ=255)
                     tgatgGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGcatca                                                                    <  2:6571003/93‑1 (MQ=255)
                       atgGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCatcatc                                                                  <  1:3856698/93‑1 (MQ=255)
                       atgGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCatcatc                                                                  >  1:3818565/1‑93 (MQ=255)
                       atgGCGCGCCCGGTAGAAGGGATGGCGGCGATTCAACGTGCGATTGACTGAGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCatcatc                                                                  >  2:3023633/1‑93 (MQ=255)
                        tgGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCAtcatca                                                                 <  1:8463045/93‑1 (MQ=255)
                        aggCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCAtcatca                                                                 <  2:70694/92‑1 (MQ=255)
                          gcgcGTCCGGTTGAAGGGATGGCGTCTATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCAcc                                                               <  2:4240162/93‑1 (MQ=255)
                          gcgcGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCAcc                                                               <  2:7170383/93‑1 (MQ=255)
                            gcgTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCgg                                                             >  1:4803690/1‑93 (MQ=255)
                             cgTCCGGTTGAAGGGATGGCGGGGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGc                                                            >  1:1532059/1‑93 (MQ=255)
                              gTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGcc                                                           >  2:3780809/1‑93 (MQ=255)
                               tCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCt                                                          >  1:2176978/1‑93 (MQ=255)
                                   gTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAGCAGCCATCAGCATCATCACCGGCCTGGTc                                                      <  2:1343520/93‑1 (MQ=255)
                                   gTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTc                                                      >  2:1155338/1‑93 (MQ=255)
                                    ttGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCg                                                     <  2:2204517/93‑1 (MQ=255)
                                     tGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGc                                                    <  2:2672558/93‑1 (MQ=255)
                                      gAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGcc                                                   <  2:7034153/93‑1 (MQ=255)
                                      gAAGGGATGGCGGCGAGTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGcc                                                   <  1:753189/93‑1 (MQ=255)
                                        aGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTg                                                 <  1:5941648/93‑1 (MQ=255)
                                         gggATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTgg                                                <  2:1851286/93‑1 (MQ=255)
                                              ggcggcGATTCAACGTGCGATTGACAGTGTTTGTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGAt                                           >  1:5621292/1‑93 (MQ=255)
                                               gcggcgATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATg                                          <  2:4112655/93‑1 (MQ=255)
                                                cggcgATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGc                                         >  1:7171876/1‑93 (MQ=255)
                                                   cgATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcg                                      >  1:8020040/1‑93 (MQ=255)
                                                    gATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcgc                                     >  1:4723195/1‑93 (MQ=255)
                                                     aTTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcgcc                                    >  2:2008138/1‑93 (MQ=255)
                                                     aTTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcgcc                                    >  2:5915741/1‑93 (MQ=255)
                                                     aTTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcgcc                                    >  1:2847584/1‑93 (MQ=255)
                                                     aTTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcgcc                                    >  1:5022925/1‑93 (MQ=255)
                                                         aaCGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCAc                                >  1:5728037/1‑93 (MQ=255)
                                                          aCGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACt                               >  2:3599577/1‑93 (MQ=255)
                                                            gTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCg                             >  2:1496420/1‑93 (MQ=255)
                                                            gTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATAATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCg                             >  2:7984141/1‑93 (MQ=255)
                                                             tGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGa                            <  1:6913107/93‑1 (MQ=255)
                                                              gCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGAt                           <  2:873783/93‑1 (MQ=255)
                                                              gCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGAt                           <  2:5132759/93‑1 (MQ=255)
                                                              gCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGAt                           <  1:8794835/93‑1 (MQ=255)
                                                              gCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGAt                           <  1:7698030/93‑1 (MQ=255)
                                                               cGATTGACAGTGTTTCTCATTATCTGGCGCGGACAACAGCCATCAGCAGCATCACCGGCCTGGTCGTCTGGTCGATGCTCGCCGCACTCGATg                          <  1:931737/93‑1 (MQ=255)
                                                                gATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGt                         <  1:4460508/93‑1 (MQ=255)
                                                                 aTTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGtt                        >  2:1754226/1‑93 (MQ=255)
                                                                 aTTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGtt                        >  2:4920367/1‑93 (MQ=255)
                                                                  ttGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttc                       >  2:668071/1‑93 (MQ=255)
                                                                    gACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgc                     <  1:1002123/93‑1 (MQ=255)
                                                                     aCAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgct                    <  2:6872441/93‑1 (MQ=255)
                                                                     aCAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgct                    >  1:230261/1‑93 (MQ=255)
                                                                     aCAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgct                    >  2:811381/1‑93 (MQ=255)
                                                                      cAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgctt                   <  1:6454487/93‑1 (MQ=255)
                                                                      cAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgctt                   >  1:96910/1‑93 (MQ=255)
                                                                      cAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgctt                   >  2:4794068/1‑93 (MQ=255)
                                                                       aGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttc                  >  1:3359065/1‑93 (MQ=255)
                                                                       aGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttc                  <  1:4705904/93‑1 (MQ=255)
                                                                        gtgtTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttcg                 >  1:7211263/1‑93 (MQ=255)
                                                                        gtgtTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttcg                 >  1:5523350/1‑93 (MQ=255)
                                                                        gtgtTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttcg                 >  2:244869/1‑93 (MQ=255)
                                                                         tgtTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttcgc                <  1:3166561/93‑1 (MQ=255)
                                                                          gtTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTtcgcttcgct               >  2:2424910/1‑93 (MQ=255)
                                                                           tttCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTcgcttcgctt              >  2:827686/1‑93 (MQ=255)
                                                                           tttCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTcgcttcgctt              >  2:3702964/1‑93 (MQ=255)
                                                                             tctcATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCtttt            <  1:638416/93‑1 (MQ=255)
                                                                              ctcATTATCTGGCGCTGAAAACATCCATCAGCATCATCGCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTg           <  2:4136069/93‑1 (MQ=255)
                                                                              ctcATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTg           <  1:7992346/93‑1 (MQ=255)
                                                                              ctcATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTg           <  1:2832497/93‑1 (MQ=255)
                                                                              ctcATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTg           <  2:3968493/93‑1 (MQ=255)
                                                                               tcATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGt          >  2:6180680/1‑93 (MQ=255)
                                                                               tcATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGt          >  1:7331469/1‑93 (MQ=255)
                                                                                cATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTc         >  2:5445575/1‑93 (MQ=255)
                                                                                cATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTc         >  1:1766406/1‑93 (MQ=255)
                                                                                 aTTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCt        >  1:1681352/1‑93 (MQ=255)
                                                                                  ttATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTg       <  1:20564/93‑1 (MQ=255)
                                                                                  ttATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTg       <  2:769790/93‑1 (MQ=255)
                                                                                   tATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTgg      <  1:2604006/93‑1 (MQ=255)
                                                                                   tATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTgg      <  1:1244662/93‑1 (MQ=255)
                                                                                   tATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTgg      >  1:4943929/1‑93 (MQ=255)
                                                                                    aTCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTggg     >  1:7567091/1‑93 (MQ=255)
                                                                                       tGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAt  <  1:6606826/93‑1 (MQ=255)
                                                                                       tGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAt  <  1:463923/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAT  >  NC_000913/1672439‑1672618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCT  >  NC_000913/1672436‑1672622
                                                                                             |                                                                                             
AATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGC                                                                                               >  SRR4302541.3021505/1‑94 (MQ=60)
AATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGC                                                                                               >  SRR4302541.3322347/1‑94 (MQ=60)
AATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGC                                                                                               <  SRR4302541.585873/94‑1 (MQ=60)
 ATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCG                                                                                              <  SRR4302541.8467167/94‑1 (MQ=60)
 ATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCG                                                                                              <  SRR4302541.2553894/94‑1 (MQ=60)
  TTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGC                                                                                             <  SRR4302541.4835281/94‑1 (MQ=60)
  TTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGC                                                                                             >  SRR4302541.4610632/1‑94 (MQ=60)
   TGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCT                                                                                            <  SRR4302541.8766160/94‑1 (MQ=60)
     CCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGA                                                                                          >  SRR4302541.1641192/1‑94 (MQ=60)
      CCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTTAACGTGCGATTGACAGTGTTTCTCATTATCTTGCGCTGAA                                                                                         <  SRR4302541.3777162/94‑1 (MQ=60)
      CCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGTGATTGACAGTGTTTCTCATTATCTGGCGCTGAA                                                                                         >  SRR4302541.8596429/1‑94 (MQ=60)
       CGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAA                                                                                        >  SRR4302541.146465/1‑94 (MQ=60)
         GAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAAC                                                                                      >  SRR4302541.2990947/1‑94 (MQ=60)
         GAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAAC                                                                                      <  SRR4302541.1039511/94‑1 (MQ=60)
          AAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACA                                                                                     >  SRR4302541.7496148/1‑94 (MQ=60)
           AAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAG                                                                                    <  SRR4302541.8527812/94‑1 (MQ=60)
           AAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAC                                                                                    >  SRR4302541.3538540/1‑94 (MQ=60)
            AATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGC                                                                                   <  SRR4302541.4898018/94‑1 (MQ=60)
             ATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAAGAGCC                                                                                  >  SRR4302541.7294664/1‑94 (MQ=60)
             ATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCC                                                                                  <  SRR4302541.936841/94‑1 (MQ=60)
             ATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCC                                                                                  <  SRR4302541.4341872/94‑1 (MQ=60)
              TTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCA                                                                                 <  SRR4302541.5834763/94‑1 (MQ=60)
               TTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCAT                                                                                >  SRR4302541.8000631/1‑94 (MQ=60)
               TTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCAT                                                                                <  SRR4302541.6575350/94‑1 (MQ=60)
                TCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATC                                                                               <  SRR4302541.3671898/94‑1 (MQ=60)
                 CAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTGTCATTATCTGGCGCTGAAAACAGCCATCA                                                                              <  SRR4302541.5652511/94‑1 (MQ=60)
                 CAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCA                                                                              <  SRR4302541.3112105/94‑1 (MQ=60)
                  cacaaattattgcggcTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCTACTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAG                                                                             <  SRR4302541.2909424/78‑1 (MQ=60)
                  AGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAG                                                                             >  SRR4302541.6820066/1‑94 (MQ=60)
                  AGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAG                                                                             <  SRR4302541.6051007/94‑1 (MQ=60)
                  AGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAG                                                                             <  SRR4302541.5532625/94‑1 (MQ=60)
                    CAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCA                                                                           <  SRR4302541.5182036/94‑1 (MQ=60)
                    CAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCA                                                                           >  SRR4302541.1856507/1‑94 (MQ=60)
                     AAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCAT                                                                          <  SRR4302541.7685913/94‑1 (MQ=60)
                     AAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCAT                                                                          <  SRR4302541.4190375/94‑1 (MQ=60)
                     AAATGATGGCGCGTCCAGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCAT                                                                          <  SRR4302541.7868110/94‑1 (MQ=60)
                     AAATGATGGCACGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCAT                                                                          <  SRR4302541.8506393/94‑1 (MQ=60)
                      AATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAtcatt                                                                         >  SRR4302541.1024473/1‑89 (MQ=60)
                       ATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCA                                                                        >  SRR4302541.1629874/1‑94 (MQ=60)
                       ATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCA                                                                        <  SRR4302541.7253820/94‑1 (MQ=60)
                        TGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCAT                                                                       >  SRR4302541.1511960/1‑94 (MQ=60)
                        TGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCAT                                                                       >  SRR4302541.4617328/1‑94 (MQ=60)
                        TGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCAT                                                                       >  SRR4302541.4532407/1‑94 (MQ=60)
                         GATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATC                                                                      >  SRR4302541.3857271/1‑94 (MQ=60)
                         GATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATC                                                                      >  SRR4302541.3857024/1‑94 (MQ=60)
                          ATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCA                                                                     >  SRR4302541.974690/1‑94 (MQ=60)
                          ATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCA                                                                     >  SRR4302541.7475629/1‑94 (MQ=60)
                          ATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCA                                                                     <  SRR4302541.3895499/94‑1 (MQ=60)
                           TGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCAC                                                                    >  SRR4302541.1095167/1‑94 (MQ=60)
                           TGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCAC                                                                    <  SRR4302541.8554333/94‑1 (MQ=60)
                            GGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACC                                                                   <  SRR4302541.6516340/94‑1 (MQ=60)
                              CGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGG                                                                 <  SRR4302541.1380510/94‑1 (MQ=60)
                              CGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGG                                                                 >  SRR4302541.4852686/1‑94 (MQ=60)
                               GCGTCCGGTTGAAGGGATGGCGGGGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGC                                                                >  SRR4302541.1547414/1‑94 (MQ=60)
                               GCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGC                                                                >  SRR4302541.1739776/1‑94 (MQ=60)
                                CGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCC                                                               >  SRR4302541.8107884/1‑94 (MQ=60)
                                 GTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCT                                                              <  SRR4302541.5172610/94‑1 (MQ=60)
                                 GTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCT                                                              >  SRR4302541.2198012/1‑94 (MQ=60)
                                  TCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTG                                                             <  SRR4302541.4686600/94‑1 (MQ=60)
                                   CCGGTTGAAGGGATGGCGGCGATTCAACGGGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGG                                                            <  SRR4302541.7636217/94‑1 (MQ=60)
                                     GGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTC                                                          <  SRR4302541.2539469/94‑1 (MQ=60)
                                      GTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCG                                                         >  SRR4302541.4356827/1‑94 (MQ=60)
                                        TGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCC                                                       >  SRR4302541.4763338/1‑94 (MQ=60)
                                        TGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCC                                                       >  SRR4302541.4763337/1‑94 (MQ=60)
                                         GAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCT                                                      <  SRR4302541.3186598/94‑1 (MQ=60)
                                         GAAGGGATGGCGGCGAGTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCT                                                      <  SRR4302541.760295/94‑1 (MQ=60)
                                          AAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTG                                                     <  SRR4302541.7703043/94‑1 (MQ=60)
                                          AAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTG                                                     <  SRR4302541.2249424/94‑1 (MQ=60)
                                           AGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGG                                                    >  SRR4302541.2768673/1‑94 (MQ=60)
                                           AGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGG                                                    <  SRR4302541.5028765/94‑1 (MQ=60)
                                           AGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGG                                                    <  SRR4302541.6002187/94‑1 (MQ=60)
                                                TGGCGGCGATTCAACGTGCGATTGACAGTGTTTGTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGAT                                               >  SRR4302541.5678813/1‑94 (MQ=60)
                                                TGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCCTTATCTGGCGCTGAAAACAGCCATCAGCATCATCGCCGGCCTGGTCGCCTGGGCGAT                                               <  SRR4302541.891720/94‑1 (MQ=60)
                                                 GGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATG                                              <  SRR4302541.8363936/94‑1 (MQ=60)
                                                  GCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGC                                             >  SRR4302541.547881/1‑94 (MQ=60)
                                                  GCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGC                                             >  SRR4302541.7248340/1‑94 (MQ=60)
                                                    GGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTC                                           >  SRR4302541.8637366/1‑94 (MQ=60)
                                                     GCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCG                                          >  SRR4302541.8108218/1‑94 (MQ=60)
                                                      CGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGC                                         >  SRR4302541.4771369/1‑94 (MQ=60)
                                                       GATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCC                                        >  SRR4302541.5074569/1‑94 (MQ=60)
                                                       GATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCC                                        >  SRR4302541.2876222/1‑94 (MQ=60)
                                                          TCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCA                                     >  SRR4302541.1500536/1‑94 (MQ=60)
                                                          TCAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCA                                     <  SRR4302541.4192658/94‑1 (MQ=60)
                                                           CAACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCAC                                    >  SRR4302541.5786427/1‑94 (MQ=60)
                                                            CACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACT                                   <  SRR4302541.7251923/94‑1 (MQ=60)
                                                            AACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACT                                   <  SRR4302541.657335/94‑1 (MQ=60)
                                                            AACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACT                                   <  SRR4302541.6688560/94‑1 (MQ=60)
                                                            AACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACT                                   <  SRR4302541.4813398/94‑1 (MQ=60)
                                                             ACGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTC                                  <  SRR4302541.4701079/94‑1 (MQ=60)
                                                              CGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCG                                 >  SRR4302541.414479/1‑94 (MQ=60)
                                                              CGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCG                                 >  SRR4302541.5687049/1‑94 (MQ=60)
                                                              CGTGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCG                                 <  SRR4302541.2866434/94‑1 (MQ=60)
                                                                TGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGAT                               <  SRR4302541.6986876/94‑1 (MQ=60)
                                                                TGCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGAT                               >  SRR4302541.3841378/1‑94 (MQ=60)
                                                                 GCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATG                              <  SRR4302541.8888780/94‑1 (MQ=60)
                                                                 GCGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATG                              <  SRR4302541.7779891/94‑1 (MQ=60)
                                                                  CGATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGT                             <  SRR4302541.940540/94‑1 (MQ=60)
                                                                  CGATTGACAGTGTTTCTCATTATCTGGCGCGGACAACAGCCATCAGCAGCATCACCGGCCTGGTCGTCTGGTCGATGCTCGCCGCACTCGATGT                             <  SRR4302541.940541/94‑1 (MQ=60)
                                                                   GATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT                            <  SRR4302541.8528021/94‑1 (MQ=60)
                                                                   GATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT                            <  SRR4302541.4505414/94‑1 (MQ=60)
                                                                   GATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT                            >  SRR4302541.2109376/1‑94 (MQ=60)
                                                                   GATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT                            <  SRR4302541.8528023/94‑1 (MQ=60)
                                                                   GATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT                            >  SRR4302541.1862998/1‑94 (MQ=60)
                                                                   GATTGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT                            >  SRR4302541.5006598/1‑94 (MQ=60)
                                                                      TGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTTGCCTGGGCGATGCTCGCCGCACTCGATGTTCGC                         >  SRR4302541.8578483/1‑94 (MQ=60)
                                                                      TGACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGC                         <  SRR4302541.7133101/94‑1 (MQ=60)
                                                                       GACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCT                        >  SRR4302541.232049/1‑94 (MQ=60)
                                                                       GACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCT                        <  SRR4302541.1011433/94‑1 (MQ=60)
                                                                       GACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCT                        >  SRR4302541.7064907/1‑94 (MQ=60)
                                                                        ACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTT                       >  SRR4302541.97741/1‑94 (MQ=60)
                                                                        ACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTT                       <  SRR4302541.330255/94‑1 (MQ=60)
                                                                        ACAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTT                       >  SRR4302541.6137505/1‑94 (MQ=60)
                                                                         CAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTC                      <  SRR4302541.6520267/94‑1 (MQ=60)
                                                                         CAGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTC                      >  SRR4302541.3392584/1‑94 (MQ=60)
                                                                          AGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCG                     <  SRR4302541.2192447/94‑1 (MQ=60)
                                                                          AGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCG                     <  SRR4302541.4753882/94‑1 (MQ=60)
                                                                          AGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCG                     >  SRR4302541.5579890/1‑94 (MQ=60)
                                                                          AGTGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCG                     >  SRR4302541.7288186/1‑94 (MQ=60)
                                                                            TGTTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCT                   <  SRR4302541.3198538/94‑1 (MQ=60)
                                                                              TTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTT                 >  SRR4302541.6712494/1‑94 (MQ=60)
                                                                              TTTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTT                 >  SRR4302541.4952147/1‑94 (MQ=60)
                                                                               TTCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTT                <  SRR4302541.5661354/94‑1 (MQ=60)
                                                                                TCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTG               <  SRR4302541.4242219/94‑1 (MQ=60)
                                                                                TCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTG               >  SRR4302541.4070185/1‑94 (MQ=60)
                                                                                TCTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTG               <  SRR4302541.644441/94‑1 (MQ=60)
                                                                                 CTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGT              >  SRR4302541.7409748/1‑94 (MQ=60)
                                                                                 CTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGT              <  SRR4302541.2861026/94‑1 (MQ=60)
                                                                                 CTCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGT              <  SRR4302541.8080334/94‑1 (MQ=60)
                                                                                  TCATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTC             >  SRR4302541.1783587/1‑94 (MQ=60)
                                                                                   CATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCT            >  SRR4302541.1697884/1‑94 (MQ=60)
                                                                                   CATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCT            <  SRR4302541.2491522/94‑1 (MQ=60)
                                                                                   CATTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCT            <  SRR4302541.5966984/94‑1 (MQ=60)
                                                                                     TTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGG          >  SRR4302541.3829904/1‑94 (MQ=60)
                                                                                     TTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGG          >  SRR4302541.4994422/1‑94 (MQ=60)
                                                                                     TTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGG          <  SRR4302541.2654868/94‑1 (MQ=60)
                                                                                     TTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGG          <  SRR4302541.20735/94‑1 (MQ=60)
                                                                                     TTATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGG          >  SRR4302541.1093498/1‑94 (MQ=60)
                                                                                      TATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGG         <  SRR4302541.1256992/94‑1 (MQ=60)
                                                                                      TATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGG         <  SRR4302541.2630068/94‑1 (MQ=60)
                                                                                      TATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGG         >  SRR4302541.7647958/1‑94 (MQ=60)
                                                                                      TATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGG         >  SRR4302541.6891410/1‑94 (MQ=60)
                                                                                       ATCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGG        >  SRR4302541.2884714/1‑94 (MQ=60)
                                                                                        TCTGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGA       <  SRR4302541.4607922/94‑1 (MQ=60)
                                                                                          TGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATT     <  SRR4302541.468341/94‑1 (MQ=60)
                                                                                          TGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATT     >  SRR4302541.2265818/1‑94 (MQ=60)
                                                                                          TGGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATT     <  SRR4302541.6674520/94‑1 (MQ=60)
                                                                                           GGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTG    >  SRR4302541.1208917/1‑94 (MQ=60)
                                                                                           GGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTG    >  SRR4302541.5051769/1‑94 (MQ=60)
                                                                                           GGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTG    >  SRR4302541.7081824/1‑94 (MQ=60)
                                                                                           GGCGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTG    >  SRR4302541.4130940/1‑94 (MQ=60)
                                                                                             CGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGac  >  SRR4302541.2666823/1‑92 (MQ=60)
                                                                                             CGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCC  <  SRR4302541.2519573/94‑1 (MQ=60)
                                                                                             CGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCC  >  SRR4302541.4216651/1‑94 (MQ=60)
                                                                                             |                                                                                             
AATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCT  >  NC_000913/1672436‑1672622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑