Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F36 I0 R1 107 8366.7 6299831 98.3% 6192733 135.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,797,481 T→G 18.3% L130V (TTA→GTA) ‡ CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,797,4810TG18.3% 140.1 / 13.8 92L130V (TTA→GTA) ‡CIW80_RS19065LacI family DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (21/53);  new base G (15/3);  total (36/56)
Fisher's exact test for biased strand distribution p-value = 2.83e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGCA  >  NZ_CP022686/3797348‑3797615
                                                                                                                                      |                                                                                                                                        
cTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAATTGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAAACAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGCCGGC‑gttatgtt                                                                                                                                    >  5:581912/1‑136 (MQ=255)
cTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGCCGCTTCTTCAAGGCAACCCGGCGGC‑gtgagtta                                                                                                                                    >  8:179940/1‑140 (MQ=255)
cTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑gtttgttt                                                                                                                                    >  8:469989/1‑139 (MQ=255)
cTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑gttagttt                                                                                                                                    >  5:757638/1‑139 (MQ=255)
cTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTAAAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGCCGGC‑gttagatt                                                                                                                                    >  7:180138/1‑137 (MQ=255)
  cccGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGACACCCGGCGGC‑ggtatttt                                                                                                                                    >  3:510594/1‑137 (MQ=255)
       tttATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTTGTTGGGTGTCATGAGTAAAATCCGCTCAAAGAAGCTGAAATTGTGATGACGCCTCGACAAGGCCGCCCGGCGGC‑GTGTGTttttttg                                                                                                                               >  4:497257/1‑137 (MQ=255)
         tATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGATTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTgg                                                                                                                             <  1:723555/138‑1 (MQ=255)
         tATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGtt                                                                                                                           <  8:565268/140‑1 (MQ=255)
          aTGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTATTTATTTTTTGttg                                                                                                                          >  6:66377/1‑139 (MQ=255)
            gCAGTCGTTGCAGCAGGTGCTTAATAAAAATGATTTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCACCCCGGCGGC‑GTGTGTGAATTTTgtgtg                                                                                                                          >  1:292222/1‑134 (MQ=255)
            gCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTGTTATTTTTGGttg                                                                                                                          >  2:471786/1‑137 (MQ=255)
             cAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTtct                                                                                                                         >  4:166966/1‑138 (MQ=255)
             cAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTGGttgt                                                                                                                         >  2:148110/1‑136 (MQ=255)
               gTCGTTGCAGAAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGCCGCTTCTTCAAGGCACCACGGCGGC‑GGTAGTTATTTTTTGTtatcctt                                                                                                                     >  7:622536/1‑137 (MQ=255)
                tCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTATTTATTTTTTGTTCTCAGtt                                                                                                                    >  8:655251/1‑140 (MQ=255)
                 cGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTg                                                                                                                   <  5:367115/140‑1 (MQ=255)
                 cGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGGTTTTTTTGGTGGTCAGGTg                                                                                                                   >  7:448022/1‑140 (MQ=255)
                  gTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCACCCCGGCGGC‑GGTTGTTTTTTTTGGTTGTCAGtt                                                                                                                    >  3:532227/1‑138 (MQ=255)
                   ttGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTg                                                                                                                   <  2:788093/138‑1 (MQ=255)
                   ttGCAGCAGGTGATTAATAAAAATGACTTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGATCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGc                                                                                                                 <  5:74509/140‑1 (MQ=255)
                     gcagcaGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGTTGTCATGATTATAATCAGCTCAAAGAAGCTGAAATTCTGAAGACGCTTCTTCCAGGCAACCAGGCGGG‑GTTACATATTTTTTGTTCACACTTGGcag                                                                                                               >  7:522602/1‑138 (MQ=255)
                         cagGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGAt                                                                                                             <  1:200949/138‑1 (MQ=255)
                         cagGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTGGTTGTCAGTTGGCTGATaa                                                                                                           >  8:329209/1‑140 (MQ=255)
                           ggTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATaaaa                                                                                                         <  6:131840/140‑1 (MQ=255)
                            gTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTGGGTCTCACGTGGCTGCTAAAAc                                                                                                        >  6:769130/1‑140 (MQ=255)
                             ggCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATaaaa                                                                                                         <  1:378690/137‑1 (MQ=255)
                                 taataaAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCa                                                                                                     <  2:36131/138‑1 (MQ=255)
                                   ataaAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTATTTTTGGGTCTCAGTTTGCTGATAAAACTcccc                                                                                                   >  2:621724/1‑138 (MQ=255)
                                    aaaaaaTGAATTCCTGCTACTGCTTTGGTGTCAAGAGTATACTCCGCTAAAAGAAGCTTAAATTCTGATGACTCTTCTTCAAGGCAAACCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCa                                                                                                  <  4:704925/137‑1 (MQ=255)
                                         aTGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATATTTTGGTGTCAGTTGGCGGTTAAAACGCCCCAGAGAc                                                                                             >  1:414207/1‑138 (MQ=255)
                                         aTGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTGGTTCTCAGTTGGGTGTTAAAACTCACCAGATAc                                                                                             >  3:721509/1‑138 (MQ=255)
                                              ttCCAGCTAGTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCgag                                                                                        <  1:476834/138‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATACTCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCgag                                                                                        <  3:151098/138‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCgag                                                                                        <  1:16669/138‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCgag                                                                                        <  3:775057/138‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCgag                                                                                        <  4:43367/138‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGaa                                                                                      <  5:560877/140‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGaa                                                                                      <  8:674861/140‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGaa                                                                                      <  6:350616/140‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGTCATGAGGATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCgag                                                                                        <  3:2447/138‑1 (MQ=255)
                                              ttCCAGCTACTGCTTGGGTGGCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGaa                                                                                      <  8:251556/140‑1 (MQ=255)
                                              ttCCAGCAACTGCTTGGGTGTCATTAGTATAATCATCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGGTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGaa                                                                                      <  7:322861/140‑1 (MQ=255)
                                              atCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTTAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCgag                                                                                        <  4:65537/137‑1 (MQ=255)
                                                  aGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACa                                                                                  <  8:107999/140‑1 (MQ=255)
                                                  aGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACa                                                                                  <  8:435910/140‑1 (MQ=255)
                                                    ctccTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACa                                                                                  <  3:523289/135‑1 (MQ=255)
                                                          cTTGGGTGTCTTGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACGAATAtacc                                                                          <  8:721675/140‑1 (MQ=255)
                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGTCTGATAAAACTCACCAGATACTCGAGAAAACTAATAtacc                                                                          <  8:718110/140‑1 (MQ=255)
                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAAtata                                                                            <  4:127498/138‑1 (MQ=255)
                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCGTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATAtacc                                                                          <  7:8990/140‑1 (MQ=255)
                                                            tGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGGTCTCATGTGGCTGCTAAAACTCACCAGATACGGGCGCAAACAAATAtaccga                                                                        >  6:351934/1‑138 (MQ=255)
                                                                    aTGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTTTTTTTTGGTCTCACTTTGCTGATTAAACTAACCAGAAAAACGAGAAAACAAAAATAAC‑TA‑CCCTTa                                                                >  1:547161/1‑138 (MQ=255)
                                                                    aTGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTTTTTTTTTTGGTTATCAGTTTGCTGGTAAAACACACCCGAGAAAGGAGAAAACAAAAATAAC‑AA‑CCGTTatc                                                              >  6:732658/1‑138 (MQ=255)
                                                                      gAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGGTTTTTTTGGTTGTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAAc                                                              >  4:31193/1‑138 (MQ=255)
                                                                         tataATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGCGGTTTGTT‑TTTTTTGTTGTGAGGTTGGAGATTAAACACACCCCATACGAGACAAAACAAAAAAACCAAA‑CCGTTAAGGtgt                                                          >  7:645523/1‑138 (MQ=255)
                                                                           taATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGt                                                         <  1:560538/138‑1 (MQ=255)
                                                                             aTCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCg                                                       <  4:100018/138‑1 (MQ=255)
                                                                             aTCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTGTTTTTTTTTGTTGTCAGTTGGGGGATTAAACTCACCAGATACTCGTGAAAACAAATATACC‑TA‑CCGGTTACGTTGTCg                                                       >  4:108230/1‑138 (MQ=255)
                                                                             aTCAGCTCAAAGAAGCTGAAATTCTGATGACGCTGCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTATTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCg                                                       <  1:338147/138‑1 (MQ=255)
                                                                              tCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTTGTTCTCAGTTGGCTGATAAAACAATCCAGATACACGAGAAAACAAATATAAC‑TA‑CCGGTAACGTTGTCgggt                                                    >  5:720746/1‑138 (MQ=255)
                                                                              tCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCCCCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTTTAGGtt                                                    >  8:365435/1‑140 (MQ=255)
                                                                               gaggtCAAAGAAGCTTAAATTGTTATGAAGCTTCTTCAAGGCAACCCGGGGGC‑GTTAGTTTTTTTTTGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGATAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTc                                                   <  8:679110/136‑1 (MQ=255)
                                                                               cAGCTCAACGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTc                                                   <  6:153942/140‑1 (MQ=255)
                                                                               cAGCTCAAAGAAGCTGAAATTCTGATGACGCTGCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTc                                                   <  8:679109/140‑1 (MQ=255)
                                                                                aGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTTTTTTTGGTTATAAGTTGGCTGATAAAACTAACCATATAATCGTTCAAAAAAATATAAA‑TA‑CCGTTATAGTTGTGGGtt                                                    >  4:83827/1‑138 (MQ=255)
                                                                                aGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTGTTATTTTTGGGTGTCAGGTGGCTGTTAAAACTAACCCGATACTGGAGAAAACAAATATAAC‑GA‑CCGTTAACGTTGTAGGtt                                                    >  4:72340/1‑138 (MQ=255)
                                                                                 gCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTTTTTTTGGGTGTGAGTTGGGTGATTAAACAGACCAGATACTCGAGTAAAAAAAATTAAC‑TA‑CCGTTAACGGTTTGGGTTtcc                                                 >  6:709426/1‑140 (MQ=255)
                                                                                   tCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTTTTTTTGGTTGTCAGTTGGCTGATAAAACTCACCAGATACTCGCGAAAACAAATATAAC‑TA‑CCGGTAACGTTGTCGGTTccc                                                 >  3:435438/1‑138 (MQ=255)
                                                                                   tCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTATTTATTTTTGGTTAGCAGTTGGGTGATAAAACTGACCAGATACTCGAGAAAAAAAATATACC‑TA‑CCGGTAAAGTTGTCgggtgcc                                                 >  3:463282/1‑133 (MQ=255)
                                                                                   tCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGt                                                                  >  2:300596/1‑121 (MQ=255)
                                                                                   tCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGt                                                                  <  1:300596/121‑1 (MQ=255)
                                                                                    cAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTATCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACCTTGTGCGTTaccc                                                >  2:43718/1‑138 (MQ=255)
                                                                                    cAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGGTGTCAGTTGGCTGGTTAAACTCCCCAGATAAGCGAGAAAACAAATATACC‑TA‑ACGTTTACGTTGTCGGTTgccc                                                >  2:673771/1‑138 (MQ=255)
                                                                                    cAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGGTGTTTTTTTTGGGTGTGAGTGGGGGGCTATAACTACCCCCCAAATCGAGCAAACAAAAAAAAC‑AA‑CCGTTAGCCTAGTGGGTTgccc                                                >  4:527057/1‑138 (MQ=255)
                                                                                       agaagCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTGTTTTTTTTTGGTGTGAGTTTGGGGGTTAAACTCCCCCCCTAAGCGCGCAAACAAAAATACC‑AACCCGTTAA‑GTTGTGGGGTGCCCgtt                                             >  1:317814/1‑138 (MQ=255)
                                                                                             tGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTcc                                       <  4:329547/138‑1 (MQ=255)
                                                                                             tGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCgg                                     <  6:649886/140‑1 (MQ=255)
                                                                                               aaaTTCTGATGAGGCTTCTTCAAGGCAACCCGGCGGA‑GTTAGTTATATTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTAGAGAAAACAAATCTACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCgg                                     <  4:491120/138‑1 (MQ=255)
                                                                                                                 ttcAAGGCAACCCGGCGGC‑GGTTGTTATTTTTGGTTGTCAGTTGGGGGATAAAACGCACCAGATACAAGAGAAAAAAAAAATACC‑AA‑CCCCTAAACTTGTGGGTGCCCCGTTAACTCCGGCTCAAATTACAATCGaaaaa                 >  6:663222/1‑138 (MQ=255)
                                                                                                                           cccGGCGGC‑GTTAGTTATATTTGGTTCTAAGTTGGCTTATAAAACACACCAGATACTCGAGAAACAAAAAATAAC‑TA‑ACGGTAACGTAGTCGGTTACCCATTCAGTCCCGATCAAAATACCATCGAAACAGCAttttttt       >  8:602882/1‑139 (MQ=255)
                                                                                                                           cccGGCGGC‑GGTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCAttttt         <  3:187786/138‑1 (MQ=255)
                                                                                                                               ggggc‑tTTAGTTATTTTTTGTTCTGAGTTGGCTGATATAACTCTCCAGTTAATCGAGATAACAAATATTCC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGTCTGAAATTACCATCGAAACAGCATTTTTTGaa     <  3:694647/136‑1 (MQ=255)
                                                                                                                               gcggc‑gTTTGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTATCGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCTAAACAGCATTTTTTGAAGc   <  5:169208/140‑1 (MQ=255)
                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGaa     <  2:317814/138‑1 (MQ=255)
                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGaa     <  1:403569/138‑1 (MQ=255)
                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGaa     <  2:462020/138‑1 (MQ=255)
                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc   <  5:663222/140‑1 (MQ=255)
                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc   <  8:622536/140‑1 (MQ=255)
                                                                                                                               gcggc‑gTTAGTTATTTTTGGGTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGATCCCCTTTCAGTGCGGCTCAAATTACCATCGAAACAGCATTTTTTGaa     <  3:430433/138‑1 (MQ=255)
                                                                                                                               gcggc‑gTTAGTTATATTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAATACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc   <  5:626583/140‑1 (MQ=255)
                                                                                                                               gcggc‑gTGAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc   <  7:50651/140‑1 (MQ=255)
                                                                                                                               gcggc‑gTGAGTTATTTTTGGTTCTCAGTTGGATGATAAAAATCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc   <  7:456233/140‑1 (MQ=255)
                                                                                                                               gcggc‑gGTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACATCATTTTTTGAAGc   <  7:343245/140‑1 (MQ=255)
                                                                                                                               gcggc‑gGTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGaa     <  3:69015/138‑1 (MQ=255)
                                                                                                                               gcctc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAAATCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCCTCGAAACAGCATTTTTTGaa     <  2:783693/134‑1 (MQ=255)
                                                                                                                                cggc‑gTTTGTTATTTTTCGATCTCAGTTGGCTTATAAAACTCACCAGATACTCGAGAAATCTAATATACC‑TA‑CCGTTATCGTTGTCGGTTCCCCTTTCTGTCCGGCTCATATTACCATCGAAACAGCATTTTTTGAAg    <  1:385090/138‑1 (MQ=255)
                                                                                                                                cggc‑gTTAGTTATGTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGCa  <  7:283901/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACTCACCAGATACTCGAGAAAACAAATATACC‑TA‑CCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGCA  >  NZ_CP022686/3797348‑3797615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A