Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,734,237 A→G 100% W156R (TGG→CGG)  papX ← HTH‑type transcriptional regulator PapX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,734,2370AG99.8% 905.5 / ‑6.5 313W156R (TGG→CGG) papXHTH‑type transcriptional regulator PapX
Reads supporting (aligned to +/- strand):  ref base A (1/1);  major base G (146/160);  minor base T (1/3);  total (149/164)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 313 total aligned reads displayed.

TCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCAGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTAAA  >  NZ_CP022686/1734101‑1734373
                                                                                                                                        |                                                                                                                                        
tCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGAt                                                                                                                                       <  5:1206488/140‑1 (MQ=255)
      ttCTCTTGGCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGTGTCAGATAATCCCGGAtttcttt                                                                                                                                 <  7:697077/140‑1 (MQ=255)
      ttCTCTTGCCTGAAGCTATGCAGCATCCTCATATATTTCGCACTTTCTGAGCTGGCAGCTGTAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGTAtttcttt                                                                                                                                 <  6:2814/140‑1 (MQ=21)
      ttCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGAtttcttt                                                                                                                                 <  1:471411/140‑1 (MQ=255)
      ttCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGAtttct                                                                                                                                   <  3:724650/138‑1 (MQ=255)
      ttCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCAATTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGAtttcttt                                                                                                                                 <  2:1251938/140‑1 (MQ=255)
      ttCTCTTGCCTGAAGCTATACAGCATCCTCAGATATTTCGCACTTTATTAGCTGTCATCAGAAATATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGatttattt                                                                                                                                 <  2:258003/140‑1 (MQ=21)
         tctTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCat                                                                                                                              >  8:443076/1‑139 (MQ=255)
         tctTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAg                                                                                                                              >  8:1217499/1‑140 (MQ=255)
         tctAGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTATATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAg                                                                                                                              >  2:782741/1‑140 (MQ=255)
           ttGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATAAATGACTTCAAACATTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGAt                                                                                                                            >  5:1054881/1‑140 (MQ=255)
             gCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATAc                                                                                                                          <  1:152471/140‑1 (MQ=255)
             gCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATAc                                                                                                                          >  8:1062107/1‑140 (MQ=255)
                tGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACa                                                                                                                         <  3:4318/138‑1 (MQ=255)
                tGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATg                                                                                                                       <  8:826730/140‑1 (MQ=255)
                tGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGGGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATg                                                                                                                       <  8:894123/140‑1 (MQ=255)
                  aaGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCATATAATCCCGGATTTCTTTCAGATACTTg                                                                                                                       <  4:1024982/138‑1 (MQ=255)
                  aaGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATg                                                                                                                       <  4:1196066/138‑1 (MQ=255)
                  aaGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCt                                                                                                                     <  7:1103474/140‑1 (MQ=255)
                  aaGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCt                                                                                                                     <  6:519256/140‑1 (MQ=255)
                  aaGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCt                                                                                                                     <  1:857276/140‑1 (MQ=255)
                  aaGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCt                                                                                                                     <  2:60337/140‑1 (MQ=255)
                  aaGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCt                                                                                                                     <  5:635123/140‑1 (MQ=255)
                  aaGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCAGCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATg                                                                                                                       <  3:221450/138‑1 (MQ=255)
                       ttCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTtgtg                                                                                                                <  8:382701/139‑1 (MQ=255)
                       aTCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTtgtg                                                                                                                >  8:218772/1‑140 (MQ=255)
                        tCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGc                                                                                                               >  5:24555/1‑140 (MQ=255)
                        tCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTATTTATTAATGACTTCAAACAGTTACTGTTCATCATGGGTCACATAATCACGGATTACTTTCAGATACATGATTTGTGc                                                                                                               >  1:581850/1‑140 (MQ=18)
                         ccAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTtgtg                                                                                                                >  3:884371/1‑138 (MQ=255)
                          cAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGc                                                                                                               >  4:287800/1‑138 (MQ=255)
                          cAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCaa                                                                                                             <  6:323309/140‑1 (MQ=255)
                               tcatCACATATTACGCACTTTATGAGCTGTAATCAGAAAAATGTGCCAGTAAATTTTTATTAATTACTTCAAAAAGTTAATTTTCATCATGGGTCAGATAATCCCGGATTTCTTTCATATACATGCTTTGTGCAAGAGTg                                                                                                        <  1:1141310/137‑1 (MQ=14)
                               tCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTg                                                                                                        >  1:132121/1‑140 (MQ=255)
                                    aGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGc                                                                                                     <  4:570088/138‑1 (MQ=255)
                                    aGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCct                                                                                                   <  5:191350/140‑1 (MQ=255)
                                    aGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGAAAGAGTGAGCct                                                                                                   >  8:553140/1‑140 (MQ=255)
                                          ttCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCAGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAAt                                                                                             <  6:179592/140‑1 (MQ=255)
                                          ttCGCACCTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAAt                                                                                             <  1:1226881/140‑1 (MQ=255)
                                           tCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTTACTTTTTATTAATGACTTCAAACAGTTCCTGTGCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATa                                                                                            <  6:1270803/140‑1 (MQ=255)
                                           tCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAAGTTTTTATTAATGACTTCAAACAGTTCCGGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGAAAGAGTGAGCCTCTGaaga                                                                                            >  6:946502/1‑138 (MQ=14)
                                           tCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCt                                                                                                                     <  5:379096/115‑1 (MQ=255)
                                           tCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATa                                                                                            >  7:990638/1‑140 (MQ=255)
                                           tCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATa                                                                                            >  5:8335/1‑140 (MQ=255)
                                           tCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAATTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATa                                                                                            >  6:78280/1‑140 (MQ=255)
                                             gCACTTTATGAGCTGTCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATg                                                                                                                       <  4:467960/111‑1 (MQ=255)
                                             gCACTTTATGAGCTGTCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATg                                                                                                                       >  3:467960/1‑111 (MQ=255)
                                             gCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATa                                                                                            >  4:1305527/1‑138 (MQ=255)
                                             gCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATa                                                                                            >  4:781000/1‑138 (MQ=255)
                                              cACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATaaaa                                                                                         <  1:1014771/140‑1 (MQ=255)
                                              cACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATaaaa                                                                                         >  6:1078897/1‑140 (MQ=255)
                                                cTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATaaaa                                                                                         >  4:1080405/1‑138 (MQ=255)
                                                   tATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACAt                                                                                    >  2:400326/1‑140 (MQ=255)
                                                   tATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACAt                                                                                    <  7:742990/140‑1 (MQ=255)
                                                   tATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACAt                                                                                    >  2:883198/1‑140 (MQ=255)
                                                   tATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTAAGATAATCCCGTATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACAt                                                                                    >  8:639572/1‑140 (MQ=255)
                                                     tGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATAc                                                                                  <  6:864391/140‑1 (MQ=255)
                                                         cTGGCATCAGAAAAATGTGCAAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGTTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTc                                                                              <  1:74543/140‑1 (MQ=255)
                                                           ggCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAg                                                                            >  2:561441/1‑140 (MQ=255)
                                                           ggCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATAAATGCTTTGTGCAAGAGTGAGCATCTGAATAAAAAACATCCCTTCAg                                                                            >  8:308724/1‑140 (MQ=255)
                                                             cATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGAtttcttt                                                                                                                                 <  3:1221795/85‑1 (MQ=255)
                                                             cATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAAc                                                                                      <  4:36310/128‑1 (MQ=255)
                                                             cATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGAt                                                                          >  1:394146/1‑140 (MQ=255)
                                                                     aaaTGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGa                                                                    >  3:1282841/1‑138 (MQ=255)
                                                                          tgCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGAt                                                               >  3:1116666/1‑138 (MQ=25)
                                                                                tAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAg                                                       <  1:220207/140‑1 (MQ=21)
                                                                                  aCTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATTGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAg                                                       <  4:692069/138‑1 (MQ=18)
                                                                                     ttttATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACgg                                                  <  2:69280/140‑1 (MQ=17)
                                                                                       ttattaATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCg                                                <  7:951600/140‑1 (MQ=17)
                                                                                       ttattaATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCg                                                <  5:689684/140‑1 (MQ=17)
                                                                                         attaATGTCTTCAAACAGTTCCTGTTCATCATCGGTCAGATAATCCAGGATTTCTTTCAGATACATTCTTAGTTCAAGAGTGAGCCTCTGAATAACAAACATACCTTCAGATGTCAGAATGATTTTTTTAGCACGGCGAt                                              <  1:778193/140‑1 (MQ=11)
                                                                                           taATGACTTCAACCAGTTACTGTTCATCATGGGTCATATTATCCCGGAGTTCTTTCAGATACATGCTTTGTGCTAGCGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGAt                                              <  3:1045922/138‑1 (MQ=11)
                                                                                              tGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGTATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGAtcctcc                                         <  6:1225146/140‑1 (MQ=17)
                                                                                              tGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGAtcctcc                                         >  7:528472/1‑140 (MQ=17)
                                                                                               gACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTccc                                        <  6:396218/140‑1 (MQ=17)
                                                                                                aCTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCt                                       <  1:242802/140‑1 (MQ=17)
                                                                                                aCTTCAAACAGTTCCTGTTCATCATGGGGCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCt                                       <  2:743481/140‑1 (MQ=17)
                                                                                                  ttCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTg                                                                                                        >  7:205739/1‑73 (MQ=21)
                                                                                                  ttCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGc                                     >  1:90013/1‑140 (MQ=17)
                                                                                                      aaCAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTa                                   >  4:206402/1‑138 (MQ=17)
                                                                                                       aCAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTAt                                  <  3:145020/138‑1 (MQ=17)
                                                                                                       aCAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATcc                                <  2:169339/140‑1 (MQ=17)
                                                                                                       aCAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATcc                                <  5:1185924/140‑1 (MQ=17)
                                                                                                         aGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCATTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGt                              <  7:449324/140‑1 (MQ=17)
                                                                                                           ttCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTcc                            >  2:1210177/1‑140 (MQ=17)
                                                                                                           ttCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTcc                            >  2:1163146/1‑140 (MQ=17)
                                                                                                           ttCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTcc                            >  5:746337/1‑140 (MQ=17)
                                                                                                            tCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATTTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTCATATCCGACCt                           >  7:1021591/1‑140 (MQ=11)
                                                                                                            tCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCt                           >  2:1279443/1‑140 (MQ=17)
                                                                                                              cTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGCCTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCt                           >  3:946909/1‑138 (MQ=17)
                                                                                                                 ttCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGCTCCTCCCTGCTATCCGTCCTTTTTa                      >  1:11011/1‑140 (MQ=17)
                                                                                                                 ttCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCttttaa                      >  5:287271/1‑138 (MQ=17)
                                                                                                                     tcatGGGTCAGATAATCCCGGATTTCTTTCAGATACATTCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTATTAATCACGGCGATCCTCCCTGCTATCAGTCCTTTTTACata                  >  8:166120/1‑140 (MQ=11)
                                                                                                                           gTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGc            >  6:713922/1‑140 (MQ=18)
                                                                                                                           gTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGc            >  1:1202801/1‑140 (MQ=18)
                                                                                                                              aGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCttt         >  1:68964/1‑140 (MQ=21)
                                                                                                                              aGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCATCCCTGCTATCCGTCCTTTTTACATATCCGGCttt         >  8:1160461/1‑140 (MQ=21)
                                                                                                                                aTAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCACTTCTATCCGTCCTTTTTACATATCCGGCttt         >  3:290508/1‑138 (MQ=255)
                                                                                                                                aTAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATAACTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACAGATCCGGCttttt       >  5:973516/1‑140 (MQ=255)
                                                                                                                                    acccGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTaa   <  6:6138/139‑1 (MQ=17)
                                                                                                                                     cccGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTaaa  >  2:1358611/1‑140 (MQ=17)
                                                                                                                                        |                                                                                                                                        
TCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCAGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTAAA  >  NZ_CP022686/1734101‑1734373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACATCTGCAAGAATCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCAGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTAAAAAATCTGTAATGC  >  NZ_CP022686/1734087‑1734386
                                                                                                                                                      |                                                                                                                                                     
CACATCTGCAAGAATCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTAGCACTTTATGAGCTGGCTACAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTAAAACAGATCCTGTTCATCATCGGTCAGATAATCCCG                                                                                                                                                       >  NB501016:372:H5LTGBGXK:2:12304:5200:5530/1‑151 (MQ=60)
   ATCTGCAAGAATCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGAT                                                                                                                                                    <  NB501016:372:H5LTGBGXK:4:12506:18605:17075/151‑1 (MQ=60)
     CTGCAAGAATCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCAGCTGATAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTT                                                                                                                                                  >  NB501016:372:H5LTGBGXK:2:21204:25747:11790/1‑151 (MQ=60)
            AATCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAG                                                                                                                                           >  NB501016:372:H5LTGBGXK:1:21102:16428:8587/1‑151 (MQ=60)
            AATCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAG                                                                                                                                           >  NB501016:372:H5LTGBGXK:2:23311:26573:15005/1‑151 (MQ=60)
            AATCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAG                                                                                                                                           >  NB501016:372:H5LTGBGXK:2:23310:1162:15015/1‑151 (MQ=60)
              TCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCTGACATTTCGCTCTTTATGAGCTGGCATCAGAAACATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGAT                                                                                                                                         <  NB501016:372:H5LTGBGXK:3:22511:13305:19378/151‑1 (MQ=60)
              TCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGAT                                                                                                                                         <  NB501016:372:H5LTGBGXK:3:23509:18656:1820/151‑1 (MQ=60)
              TCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGAT                                                                                                                                         >  NB501016:372:H5LTGBGXK:1:23112:11993:8488/1‑151 (MQ=60)
              TCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATAAATGACTTCAAACATTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGAT                                                                                                                                         >  NB501016:372:H5LTGBGXK:3:22606:2604:17210/1‑151 (MQ=60)
                ATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATAC                                                                                                                                       >  NB501016:372:H5LTGBGXK:1:11112:13149:4305/1‑151 (MQ=60)
                 TTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACA                                                                                                                                      >  NB501016:372:H5LTGBGXK:1:13303:13196:19821/1‑151 (MQ=60)
                   ATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTACCTGTTCATCATGGGTCTGATAATCCCGGATTTCTTTCAGATACATG                                                                                                                                    >  NB501016:372:H5LTGBGXK:4:11501:14632:9900/1‑151 (MQ=60)
                    TTCTCTTGGCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGTGTCAGATAATCCCGGATTTCTTTCAGATACATGC                                                                                                                                   <  NB501016:372:H5LTGBGXK:4:21411:7936:10695/151‑1 (MQ=60)
                    TTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGC                                                                                                                                   <  NB501016:372:H5LTGBGXK:2:21108:8205:2822/151‑1 (MQ=60)
                    TTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGC                                                                                                                                   <  NB501016:372:H5LTGBGXK:1:13102:19383:5473/151‑1 (MQ=60)
                    TTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGC                                                                                                                                   <  NB501016:372:H5LTGBGXK:2:22303:3987:10966/151‑1 (MQ=60)
                    TTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGC                                                                                                                                   <  NB501016:372:H5LTGBGXK:1:12102:12904:1258/151‑1 (MQ=60)
                    TTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGC                                                                                                                                   <  NB501016:372:H5LTGBGXK:3:11501:1768:14997/151‑1 (MQ=60)
                      gataacgtctcgtgggctcggagatgtgtataagagacagCTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTT                                                                                                                                 <  NB501016:372:H5LTGBGXK:2:23203:21512:18801/111‑1 (MQ=60)
                      CTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTT                                                                                                                                 <  NB501016:372:H5LTGBGXK:2:12104:12024:9107/151‑1 (MQ=60)
                      CTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTT                                                                                                                                 <  NB501016:372:H5LTGBGXK:2:13107:23629:6833/151‑1 (MQ=60)
                        CTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTG                                                                                                                               <  NB501016:372:H5LTGBGXK:1:11308:11044:8649/151‑1 (MQ=60)
                          TGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTG                                                                                                                             <  NB501016:372:H5LTGBGXK:3:11605:14824:5166/151‑1 (MQ=60)
                          TGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTG                                                                                                                             >  NB501016:372:H5LTGBGXK:2:21309:6093:14110/1‑151 (MQ=60)
                          TGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTG                                                                                                                             >  NB501016:372:H5LTGBGXK:3:12504:21861:16805/1‑151 (MQ=60)
                           GCCTGAAGCTATCCCGCATCCTCATATATTTCGCACTTTATGATCTTGCATCAGAAAAATGTGCCAGTTACTTTTTATTTATGAATGCAAAAAGTTACTGTTCTTCTTGTGTCAGATATTCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            <  NB501016:372:H5LTGBGXK:4:12601:2768:16641/151‑1 (MQ=60)
                           GCCTGAAGCTATCCAGCATCGTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTAAATCAGTTCCTGATCATCATGGGTCAGATTATCTCGGATTTCTTTCAGATACATGCTTTGGGC                                                                                                                            >  NB501016:372:H5LTGBGXK:2:23203:1493:13369/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCATATACATGCTTTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:1:21206:9711:1089/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            <  NB501016:372:H5LTGBGXK:1:11212:26022:11556/151‑1 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:1:22203:3752:12974/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:3:12409:10100:5486/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:2:23301:17430:5766/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:4:13406:24225:9364/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:3:11405:10758:9185/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:4:21503:12377:6414/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGC                                                                                                                            <  NB501016:372:H5LTGBGXK:3:11407:18067:1359/151‑1 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCATTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:1:22305:24739:10482/1‑151 (MQ=60)
                           GCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTATTTATTAATGACTTCAAACAGTTACTGTTCATCATGGGTCACATAATCACGGATTACTTTCAGATACATGATTTGTGC                                                                                                                            >  NB501016:372:H5LTGBGXK:1:13207:3911:3075/1‑151 (MQ=60)
                              TGAAGCTATCCTGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAG                                                                                                                         <  NB501016:372:H5LTGBGXK:2:11101:19987:14615/151‑1 (MQ=60)
                              TGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAG                                                                                                                         <  NB501016:372:H5LTGBGXK:2:11101:19995:14606/151‑1 (MQ=60)
                                tgtataagagacagCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:3:12603:6798:10048/137‑1 (MQ=60)
                                gtgtataagagacagCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:1:11112:4223:7552/136‑1 (MQ=60)
                                ccGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGTCATCAGAAAAATGTGGCAGTATCTTTTTATTACTGACTTCAATCAGTTCCTGTTCATCTTGGGTCAGATAATCCCGGATTTCTTTCAGATTCATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:3:12403:26056:18920/149‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCATATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:3:21404:1770:6172/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCCTCAGAAAAATGTTCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGTATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:1:12105:25183:14498/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:2:21312:3356:9867/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:4:23410:17846:2029/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:2:13102:11967:12584/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:3:23507:18056:12855/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       >  NB501016:372:H5LTGBGXK:2:22110:18694:3668/1‑151 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       >  NB501016:372:H5LTGBGXK:4:22509:7248:4758/1‑151 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:4:12411:16690:5636/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:3:22411:22940:7178/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:3:22411:15159:16596/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:1:23108:12667:19236/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:3:21604:4759:12019/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:4:22504:2949:16358/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:3:13608:22953:19692/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:1:21302:12660:17606/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:4:11402:8985:18582/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:1:21307:18917:18481/151‑1 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTATTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTAAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       >  NB501016:372:H5LTGBGXK:3:22609:13261:13549/1‑151 (MQ=60)
                                AAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCAGCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:2:11312:15658:1681/151‑1 (MQ=60)
                                AAGCTATCCAGCATACTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAG                                                                                                                       <  NB501016:372:H5LTGBGXK:1:13202:1901:12515/151‑1 (MQ=60)
                                  GCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTG                                                                                                                     >  NB501016:372:H5LTGBGXK:1:12306:11097:10297/1‑151 (MQ=60)
                                  GCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTG                                                                                                                     >  NB501016:372:H5LTGBGXK:1:11209:11447:5219/1‑151 (MQ=60)
                                   CTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCACAGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGA                                                                                                                    >  NB501016:372:H5LTGBGXK:3:21404:4906:12501/1‑151 (MQ=60)
                                     ATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAGGAGTGAGC                                                                                                                  >  NB501016:372:H5LTGBGXK:3:12402:13170:17422/1‑151 (MQ=60)
                                     ATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGC                                                                                                                  >  NB501016:372:H5LTGBGXK:1:22210:22365:10477/1‑151 (MQ=60)
                                      TCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCC                                                                                                                 <  NB501016:372:H5LTGBGXK:2:23202:18035:17034/151‑1 (MQ=60)
                                             TCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAAT                                                                                                          >  NB501016:372:H5LTGBGXK:3:12605:13558:19283/1‑151 (MQ=60)
                                             TCCTAAGATATTTCGCTCTTTATGAGATGTCATCATAAAAATGTGGCAGTAACTTTTTATTAATGACTTCATACAGTTCCTGTTCATCATGGTTCAGATTATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAAT                                                                                                          <  NB501016:372:H5LTGBGXK:4:13609:5683:2344/151‑1 (MQ=43)
                                             TCATCACATATTACGCACTTTATGAGCTGTAATCAGAAAAATGTGCCAGTAAATTTTTATTAATTACTTCAAAAAGTTAATTTTCATCATGGGTCAGATAATCCCGGATTTCTTTCATATACATGCTTTGTGCAAGAGTGAGCCTCTGAAT                                                                                                          <  NB501016:372:H5LTGBGXK:1:22311:24856:1075/151‑1 (MQ=41)
                                              CCTCAGATATTTCGCACTTTATGAGCTGTCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGctgtctcttataca                                                                                                         >  NB501016:372:H5LTGBGXK:2:13102:14128:18502/1‑137 (MQ=60)
                                              CCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATA                                                                                                         >  NB501016:372:H5LTGBGXK:4:22602:26045:5905/1‑151 (MQ=60)
                                              CCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATA                                                                                                         >  NB501016:372:H5LTGBGXK:3:11402:18528:8844/1‑151 (MQ=60)
                                               CTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCACGGATTTCTTTCAGATACATGCTTTGTGCAAGAGAGAGCCTCTGAATAA                                                                                                        >  NB501016:372:H5LTGBGXK:1:23304:17939:1419/1‑151 (MQ=60)
                                               CTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTACAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAA                                                                                                        >  NB501016:372:H5LTGBGXK:2:23204:9918:8317/1‑151 (MQ=60)
                                                 CAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAA                                                                                                      >  NB501016:372:H5LTGBGXK:1:23308:8256:12735/1‑151 (MQ=60)
                                                  AGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAA                                                                                                     <  NB501016:372:H5LTGBGXK:3:11608:26366:8234/151‑1 (MQ=60)
                                                      ATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGCTAACTGCATTGTGCAAGAGTGAGCCTCTTAATAAAAcacct                                                                                                 >  NB501016:372:H5LTGBGXK:4:21504:24311:19143/1‑146 (MQ=60)
                                                      ATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACAT                                                                                                 >  NB501016:372:H5LTGBGXK:4:11609:2241:6448/1‑151 (MQ=60)
                                                        TTCGCACCTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATAC                                                                                               <  NB501016:372:H5LTGBGXK:1:23112:22764:9479/151‑1 (MQ=60)
                                                           GCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTT                                                                                            <  NB501016:372:H5LTGBGXK:4:13505:23059:11260/151‑1 (MQ=60)
                                                            CACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTC                                                                                           <  NB501016:372:H5LTGBGXK:1:22202:18020:19770/151‑1 (MQ=60)
                                                            CACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTC                                                                                           <  NB501016:372:H5LTGBGXK:3:11401:14096:10106/151‑1 (MQ=60)
                                                              CTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAG                                                                                         <  NB501016:372:H5LTGBGXK:4:23503:7301:15741/151‑1 (MQ=60)
                                                              CTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAG                                                                                         >  NB501016:372:H5LTGBGXK:2:11106:1432:17810/1‑151 (MQ=60)
                                                              CTTAATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAC                                                                                         >  NB501016:372:H5LTGBGXK:2:12308:14032:13926/1‑151 (MQ=60)
                                                                TTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTGGTGCAAGAGTGAGCCTCTGAATAAAAAACATACATTCAGAT                                                                                       >  NB501016:372:H5LTGBGXK:1:12301:22580:20371/1‑151 (MQ=60)
                                                                TTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTAAAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATCCCTTCAGAT                                                                                       >  NB501016:372:H5LTGBGXK:1:21303:8121:1385/1‑151 (MQ=60)
                                                                 TATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATG                                                                                      <  NB501016:372:H5LTGBGXK:4:21507:8111:15236/151‑1 (MQ=60)
                                                                      GCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGA                                                                                 >  NB501016:372:H5LTGBGXK:2:23302:13690:1737/1‑151 (MQ=60)
                                                                       CTGGCATCAGAAAAATGTGCCAGTAACTTTTTATAAATGACTTCAAACAGTTCCTGTTAATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGGGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGAC                                                                                <  NB501016:372:H5LTGBGXK:3:23609:26622:15004/151‑1 (MQ=54)
                                                                       CTGGCATCAGAAAAATGTGCAAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGTTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGAC                                                                                <  NB501016:372:H5LTGBGXK:1:11112:24497:3502/151‑1 (MQ=52)
                                                                       CTAGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTGAAACAGTTCCTGTTCATCATGGGTAAGTTAATCCAGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGAC                                                                                <  NB501016:372:H5LTGBGXK:2:21202:3637:2688/151‑1 (MQ=46)
                                                                         GGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTG                                                                              >  NB501016:372:H5LTGBGXK:3:13611:6617:13005/1‑151 (MQ=54)
                                                                           CATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGAT                                                                            >  NB501016:372:H5LTGBGXK:2:22310:18287:18706/1‑151 (MQ=50)
                                                                                 AAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTT                                                                      <  NB501016:372:H5LTGBGXK:1:23108:22586:7008/151‑1 (MQ=38)
                                                                                    AATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGGCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGC                                                                   <  NB501016:372:H5LTGBGXK:4:12611:7639:3971/151‑1 (MQ=31)
                                                                                      TGTGCCAGTAACTTTTTTTTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCAC                                                                 <  NB501016:372:H5LTGBGXK:1:11302:1778:1790/151‑1 (MQ=27)
                                                                                              TAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACCTACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCC                                                         <  NB501016:372:H5LTGBGXK:4:22407:22060:8876/151‑1 (MQ=18)
                                                                                              TAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCC                                                         <  NB501016:372:H5LTGBGXK:1:11311:22052:4460/151‑1 (MQ=19)
                                                                                                ACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTC                                                       <  NB501016:372:H5LTGBGXK:1:11311:5527:8282/151‑1 (MQ=12)
                                                                                                 CTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCC                                                      >  NB501016:372:H5LTGBGXK:4:13506:21993:13808/1‑151 (MQ=10)
                                                                                                   TTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGCTCCTCCCT                                                    >  NB501016:372:H5LTGBGXK:1:13309:15257:2850/1‑151 (MQ=7)
                                                                                                     TTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATctgtctcttatacacatctccgagcccacgagacgttatcgaatcgggggttgggg                                                  >  NB501016:372:H5LTGBGXK:4:11611:2610:15901/1‑95 (MQ=10)
                                                                                                     TTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGC                                                  >  NB501016:372:H5LTGBGXK:1:11202:9773:12306/1‑151 (MQ=7)
                                                                                                     TTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGC                                                  <  NB501016:372:H5LTGBGXK:3:21406:3901:9152/151‑1 (MQ=7)
                                                                                                     TTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGC                                                  <  NB501016:372:H5LTGBGXK:1:21306:15165:18138/151‑1 (MQ=7)
                                                                                                     TTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGC                                                  <  NB501016:372:H5LTGBGXK:4:22507:8707:14370/151‑1 (MQ=7)
                                                                                                       ATTAATGTCTTCAAACAGTTCCTGTTCATCATCGGTCAGATAATCCAGGATTTCTTTCAGATACATTCTTAGTTCAAGAGTGAGCCTCTGAATAACAAACATACCTTCAGATGTCAGAATGATTTTTTTAGCACGGCGATCCTCCCTGCTA                                                <  NB501016:372:H5LTGBGXK:1:21202:21305:8451/151‑1 (MQ=5)
                                                                                                         TAATGACTTCAACCAGTTACTGTTCATCATGGGTCATATTATCCCGGAGTTCTTTCAGATACATGCTTTGTGCTAGCGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATC                                              <  NB501016:372:H5LTGBGXK:2:22211:6371:19709/151‑1 (MQ=6)
                                                                                                         TAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATC                                              <  NB501016:372:H5LTGBGXK:1:13203:24172:18176/151‑1 (MQ=7)
                                                                                                         TAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATC                                              <  NB501016:372:H5LTGBGXK:3:23508:7309:4745/151‑1 (MQ=7)
                                                                                                          AATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCC                                             <  NB501016:372:H5LTGBGXK:2:11212:24039:10715/151‑1 (MQ=7)
                                                                                                          AATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCC                                             <  NB501016:372:H5LTGBGXK:1:23202:12158:9757/151‑1 (MQ=7)
                                                                                                          AATGACATCATCCAGTTCCTGTGCATCATGGGGCAGATAATCCCGGAGATCTTTCAGATACATGCTTTTTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCC                                             <  NB501016:372:H5LTGBGXK:3:11401:9645:16529/151‑1 (MQ=6)
                                                                                                            TGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATATTCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGT                                           <  NB501016:372:H5LTGBGXK:4:13509:9849:5815/151‑1 (MQ=7)
                                                                                                              ACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCC                                         >  NB501016:372:H5LTGBGXK:1:12102:22351:10645/1‑151 (MQ=7)
                                                                                                              ACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCC                                         <  NB501016:372:H5LTGBGXK:1:12102:10133:11695/151‑1 (MQ=7)
                                                                                                              ACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCC                                         >  NB501016:372:H5LTGBGXK:3:21504:8605:18016/1‑151 (MQ=7)
                                                                                                              ACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCC                                         >  NB501016:372:H5LTGBGXK:2:12108:14304:16466/1‑151 (MQ=7)
                                                                                                              ACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCC                                         >  NB501016:372:H5LTGBGXK:4:23403:2830:10673/1‑151 (MQ=7)
                                                                                                              ACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCGCAGAATAAAAAACAAACCTTCAGATGTCAGACTGAATTTTTTAGCACGACGATCATCCCTGCTATCCGTCC                                         >  NB501016:372:H5LTGBGXK:2:11101:9192:1701/1‑151 (MQ=6)
                                                                                                               CTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATTTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTCATATCCGACCT                                        >  NB501016:372:H5LTGBGXK:4:22607:19787:11357/1‑151 (MQ=7)
                                                                                                               CTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGCCTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCT                                        >  NB501016:372:H5LTGBGXK:2:22107:12946:13518/1‑151 (MQ=7)
                                                                                                               CTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGCTTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCT                                        >  NB501016:372:H5LTGBGXK:4:22509:9587:5141/1‑151 (MQ=7)
                                                                                                                TTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTT                                       <  NB501016:372:H5LTGBGXK:1:21206:13281:11843/151‑1 (MQ=7)
                                                                                                                   AAACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTT                                    >  NB501016:372:H5LTGBGXK:1:21101:18352:14181/1‑151 (MQ=7)
                                                                                                                    AACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGCTCCTCCCTGCTATCCGTCCTTTTTA                                   >  NB501016:372:H5LTGBGXK:1:11102:1773:12209/1‑151 (MQ=7)
                                                                                                                    AACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTA                                   >  NB501016:372:H5LTGBGXK:1:22305:26387:19347/1‑151 (MQ=7)
                                                                                                                    AACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTAA                                   >  NB501016:372:H5LTGBGXK:3:12501:4507:5347/1‑151 (MQ=7)
                                                                                                                     ACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTAC                                  <  NB501016:372:H5LTGBGXK:3:23505:9533:15064/151‑1 (MQ=7)
                                                                                                                     ACAGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTAC                                  <  NB501016:372:H5LTGBGXK:2:11212:22381:9678/151‑1 (MQ=7)
                                                                                                                       AGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACAT                                <  NB501016:372:H5LTGBGXK:1:12212:19573:14541/151‑1 (MQ=7)
                                                                                                                       AGTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCATTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACAT                                <  NB501016:372:H5LTGBGXK:4:13405:7499:5892/151‑1 (MQ=7)
                                                                                                                        GTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCGTTTTTACATA                               >  NB501016:372:H5LTGBGXK:2:22104:17664:1792/1‑151 (MQ=7)
                                                                                                                        GTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATA                               >  NB501016:372:H5LTGBGXK:3:23502:16064:8721/1‑151 (MQ=7)
                                                                                                                        GTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATA                               >  NB501016:372:H5LTGBGXK:2:22212:22878:14042/1‑151 (MQ=7)
                                                                                                                        GTTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATA                               >  NB501016:372:H5LTGBGXK:3:11608:16095:6186/1‑151 (MQ=7)
                                                                                                                         TTCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATAT                              >  NB501016:372:H5LTGBGXK:1:12306:7874:19516/1‑151 (MQ=7)
                                                                                                                          TCCTGTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATC                             <  NB501016:372:H5LTGBGXK:1:23102:16882:9324/151‑1 (MQ=7)
                                                                                                                              GTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGC                         >  NB501016:372:H5LTGBGXK:1:23108:14916:15371/1‑151 (MQ=10)
                                                                                                                              GTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGC                         >  NB501016:372:H5LTGBGXK:2:12312:11522:2254/1‑151 (MQ=10)
                                                                                                                              GTTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGC                         >  NB501016:372:H5LTGBGXK:3:21411:15935:16924/1‑151 (MQ=10)
                                                                                                                               TTCATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCT                        <  NB501016:372:H5LTGBGXK:1:23301:9231:13137/151‑1 (MQ=12)
                                                                                                                                 CATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTT                      >  NB501016:372:H5LTGBGXK:1:11111:12277:6495/1‑151 (MQ=19)
                                                                                                                                 CATCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCACTTCTATCCGTCCTTTTTACATATCCGGCTTT                      >  NB501016:372:H5LTGBGXK:2:12111:14641:2281/1‑151 (MQ=18)
                                                                                                                                   TCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTT                    >  NB501016:372:H5LTGBGXK:3:21512:21512:14044/1‑151 (MQ=21)
                                                                                                                                   TCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTT                    >  NB501016:372:H5LTGBGXK:2:13108:11460:8730/1‑151 (MQ=21)
                                                                                                                                   TCATGGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATAACTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACAGATCCGGCTTTTT                    >  NB501016:372:H5LTGBGXK:3:22506:2619:6716/1‑151 (MQ=20)
                                                                                                                                       GGGTCAGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGGTATCCGTCCGTTTTACATATCCGGCTTTTTCTAA                >  NB501016:372:H5LTGBGXK:2:11302:19609:20085/1‑151 (MQ=20)
                                                                                                                                            AGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCctgtctcttatacacatctccgagcccacgagacgttatcga           >  NB501016:372:H5LTGBGXK:1:21202:4302:14580/1‑109 (MQ=9)
                                                                                                                                            AGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTAAATAAT           >  NB501016:372:H5LTGBGXK:4:23507:9126:6852/1‑151 (MQ=21)
                                                                                                                                            AGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTAAAAAAT           >  NB501016:372:H5LTGBGXK:2:13108:15720:12594/1‑151 (MQ=21)
                                                                                                                                            AGATAATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTATGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCAGGATTTTTCTAAAAAAA           >  NB501016:372:H5LTGBGXK:3:12606:24714:3424/1‑151 (MQ=7)
                                                                                                                                                 ATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCtgtctcttatacacatctccgagcccacgagacgttatc      >  NB501016:372:H5LTGBGXK:3:23412:23560:9890/1‑112 (MQ=0)
                                                                                                                                                 ATCCCGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTAAAAAATCTGTA      >  NB501016:372:H5LTGBGXK:2:23107:8160:6315/1‑151 (MQ=10)
                                                                                                                                                     CGGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTAAAAAATCTGTAATGC  <  NB501016:372:H5LTGBGXK:2:21212:13223:3326/151‑1 (MQ=8)
                                                                                                                                                      |                                                                                                                                                     
CACATCTGCAAGAATCATTATTCTCTTGCCTGAAGCTATCCAGCATCCTCAGATATTTCGCACTTTATGAGCTGGCATCAGAAAAATGTGCCAGTAACTTTTTATTAATGACTTCAAACAGTTCCTGTTCATCATGGGTCAGATAATCCCAGATTTCTTTCAGATACATGCTTTGTGCAAGAGTGAGCCTCTGAATAAAAAACATACCTTCAGATGTCAGACTGATTTTTTTAGCACGGCGATCCTCCCTGCTATCCGTCCTTTTTACATATCCGGCTTTTTCTAAAAAATCTGTAATGC  >  NZ_CP022686/1734087‑1734386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑