Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F2 I0 R1 181 7914.3 8336376 98.4% 8202993 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,954,955 T→G 18.3% Q81P (CAG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,954,9550TG18.3% 273.3 / 9.7 156Q81P (CAG→CCG) ttdRDNA‑binding transcriptional activator TtdR
Reads supporting (aligned to +/- strand):  ref base T (46/75);  new base G (30/2);  total (79/77)
Fisher's exact test for biased strand distribution p-value = 4.44e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

CACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGC  >  NZ_CP022686/1954816‑1955094
                                                                                                                                           |                                                                                                                                            
         cTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTggg                                                                                                                                    <  6:142908/140‑1 (MQ=255)
           cAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTg                                                                                                                                  <  4:552115/140‑1 (MQ=255)
           cAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTg                                                                                                                                  <  5:872529/140‑1 (MQ=255)
           cAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTg                                                                                                                                  <  2:155910/140‑1 (MQ=255)
             ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGGGTGACGTCATCGACCAGGGGCTG‑ATATTTGGTGAg                                                                                                                                >  4:451162/1‑140 (MQ=255)
             ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTGTTGATTTGCGTGACCTCATAGACCAGTCGCTG‑ATATTGGGTGAg                                                                                                                                >  7:721828/1‑140 (MQ=255)
              gATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGGGAgg                                                                                                                               >  2:651907/1‑140 (MQ=255)
               aTAATTGCGCATCAGGTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGa                                                                                                                              <  6:888355/140‑1 (MQ=255)
                   ttGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGCTTTGCGTTGCCTCATAGACCAGTCGACG‑ATATTTGGTGCGGAtttg                                                                                                                          >  4:986123/1‑139 (MQ=255)
                   ttGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCACCGGGCGCGTTTTTATTTTCGTGACGTCATCCACCAGTAGCTGAATATGGGGT‑AGGAttta                                                                                                                          >  5:233968/1‑139 (MQ=255)
                   ttGCGCATCAGATCGGTAATAGCAGGCGCAATATGGCTGCGCCCAACACCAAAACTGAAGCCAATGAGGATCATCCCCTCCGGGCCCGGTGTGAGTTTCGGGACCGCTTCGTCCAGTCGGGG‑TGAGTGGGGGAGGAtgtg                                                                                                                          >  6:962309/1‑137 (MQ=255)
                      cgcATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTATTTTCGGGACGTCCACGCCCAGGCGCGG‑ATATTGGGGGTGGTTTTCCaa                                                                                                                       >  6:157003/1‑139 (MQ=255)
                      cgcATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGACGTCATCGACCAGTCGCGG‑ATATTGGGTGAGGATTTACac                                                                                                                       >  1:231504/1‑139 (MQ=255)
                        cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGGGTGACGTCATCGACCAGTCGCGG‑TTATTGGGTGATGTTTTGCcgcg                                                                                                                     >  5:807566/1‑140 (MQ=255)
                         aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAgcgc                                                                                                                    <  1:223568/140‑1 (MQ=255)
                         aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGGGAGGATTTCCAgcgc                                                                                                                    <  5:584651/140‑1 (MQ=255)
                           cAGTTCGGTAATAGCTGGCGCAATATTGAGGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATACCCTCCGGGCGCGTTTTGATTTGCGTGTCGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCAt                                                                                                                  <  6:603633/140‑1 (MQ=255)
                              ttCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCTTCCCCTCCGGGCGCGGTTTTATTTGCGTGCAGTCAACCACCAGTAGCTG‑TTATTGGGTGCGGATTTCCTGCGCATggt                                                                                                               >  2:1003375/1‑138 (MQ=255)
                              ttCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGtt                                                                                                               <  3:891664/140‑1 (MQ=255)
                                cGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGACGTCATCGACCAGTCGCGG‑ATATTTGGTGAGGATTTACAGCGCaaggtgg                                                                                                             >  4:761978/1‑134 (MQ=255)
                                   tAATAGCTGGCGCAATATGGCTGCGCCCAAATCCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCGG‑TTATTGTGTGTGGTTTTACATCGGATGTTAGTaa                                                                                                          >  4:247028/1‑140 (MQ=255)
                                     aTAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCCTCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTaaca                                                                                                        <  6:730457/140‑1 (MQ=255)
                                        gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGTTTTAGGATTTCCAGCGCATGTTCGTatgatag                                                                                                     <  6:902868/140‑7 (MQ=255)
                                        gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACCTCATCGACCAGTCGCTG‑ATATTTGGTGAGGATTTACAGCGCAAGTTCGTtaaaacc                                                                                                     >  2:31700/1‑139 (MQ=255)
                                        gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGTTTTGCGGGACGTCATAGACCAGTCGCGG‑ATATTGGGGGTGGATTTCCAGCGCATGTTCGTaacaacc                                                                                                     >  1:858092/1‑139 (MQ=255)
                                           ggCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGGGTGACGTCATCGACCAGTCGGGG‑ATATTGGGTGAGGATTTGCAGCGCATGTTGGTAACAACGCTg                                                                                                  >  5:453171/1‑140 (MQ=255)
                                           ggCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGGGTGACGTCATCGGCCAGTCGCGG‑ATATTTGGTGAGGGTTTGCAGCGCCTGTTGGTTACAACGCTg                                                                                                  >  5:614464/1‑140 (MQ=255)
                                           ggCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGGTTGAATTGAGTGCCGGCATCGCCCCGTCGCGG‑AGTTTGGGGGGGGGTTTGCAGCCGATGGTCGGtacaacccgg                                                                                                  >  1:103738/1‑136 (MQ=255)
                                             cgcAATATGGCTGCGCCCAAAACCTAACCTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                                                                <  1:679122/140‑1 (MQ=255)
                                             cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                                                                <  7:54352/140‑1 (MQ=255)
                                             cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                                                                <  3:1003180/140‑1 (MQ=255)
                                             cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                                                                <  7:982895/140‑1 (MQ=255)
                                             cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                                                                <  8:229271/140‑1 (MQ=255)
                                             cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                                                                >  3:542441/1‑140 (MQ=255)
                                             cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGGCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                                                                <  1:672531/140‑1 (MQ=255)
                                             cgcAATATGGCGGCGCCCAAAACCAAAACTGCAACCACTGCGGAGCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑AGATTGGGTGAGGATTTCCCGCGCAGGTGCGTAACAACGCTGGc                                                                                                <  8:324766/140‑1 (MQ=255)
                                                  tatGTCTGCGCCCAAAACCTAAACTGCACCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTGCGTAACAACGCTGGCCACtt                                                                                           <  8:426491/140‑1 (MQ=255)
                                                      gCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTtcag                                                                                       >  8:685557/1‑140 (MQ=255)
                                                       cTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGGGGGACGTCATCGGCCAGGCGCGG‑ATATTTGGTGTGGCTTTGCCACGCATGTTGGTAACAACACGGGCCACATtgagt                                                                                      >  3:960100/1‑140 (MQ=255)
                                                       cTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTtcagt                                                                                      <  8:443572/140‑1 (MQ=255)
                                                          cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTTTTTGCGGGGCGTCATCGCCCCCTCGCTG‑ATATTGTGTGATGATTTCCCGCCGATTGTAGTAACAACACAGGCCCCTTtcaggcag                                                                                   >  6:722326/1‑140 (MQ=255)
                                                          cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTATTTGCGGTACCTCATCCACCAGTCCCAG‑ATATTTGGTGACGGTTTACCGCCCAAGTTCCTAACAACCCGGGCCCCATtaagacag                                                                                   >  2:848604/1‑140 (MQ=255)
                                                          cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGTTTTGGGGGTCGTCATCGCCCCGGCGCGG‑ATATTGGGTGTGGGTTTCCATCGCATGGTGGTTAAACCGGGGGGCACGTtcggtcag                                                                                   >  7:974758/1‑140 (MQ=255)
                                                          cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGCCCTCCTCGACCAGTCGCGG‑ATAATTGGGGAGGATTTCCAGGGCATGTTCGTAACAACGCGTGACACTTtcagtccg                                                                                   >  4:767900/1‑138 (MQ=255)
                                                          cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGGTG‑ATATTGGGTGAGGATTTACAGCGCATGTTCGTAACAACGCTGGCCACTTtcagtcag                                                                                   >  4:913666/1‑140 (MQ=255)
                                                          cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGCCGTCATAGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCCGCGCATGTTAGTAACAACGCTGGCCACGTtcagtcag                                                                                   >  2:374572/1‑140 (MQ=255)
                                                             ccaaaaccaaaaCTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAgcgc                                                                                <  1:493390/140‑1 (MQ=255)
                                                             ccaaaaccaaaaCAGCAACCAATGCGGATCATCCCCTCCGGGCGCGTGTTGATTTGCGTGACGTCATCGACCAGGCGCTG‑ATATTGGGTGCGGAGATCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAgcgc                                                                                <  2:608489/140‑1 (MQ=255)
                                                              caaaaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGcc                                                                               <  4:270325/140‑1 (MQ=255)
                                                                        acagCACCCAATCCGCATCATCCCCTCCGGGCGCGTTTTGATTTGCGAGACGTCATCGACCAGTCGCTG‑ATCTTGGGGGAGGATTTCCAGCGCATGTTCGTAACAACGCGGGCCACTTTCAGTCAGCGCCACGCCGCgag                                                                     <  7:624208/137‑1 (MQ=255)
                                                                           gCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                                                                  <  1:764322/140‑1 (MQ=255)
                                                                            cAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ata                                                                                                                                         >  4:590758/1‑68 (MQ=255)
                                                                             acccAATGCGGATCCTCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                                                                <  6:1014372/138‑1 (MQ=255)
                                                                             aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                                                                <  4:21087/140‑1 (MQ=255)
                                                                             aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                                                                <  6:871351/140‑1 (MQ=255)
                                                                             aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                                                                <  2:858092/140‑1 (MQ=255)
                                                                             aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                                                                <  8:299559/140‑1 (MQ=255)
                                                                             aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                                                                <  3:506905/140‑1 (MQ=255)
                                                                                cAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGagcggagcgg                                                             <  2:272609/140‑1 (MQ=255)
                                                                                cAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTGCGTAACAACGCTGGCCTCTTTCAGTCCGCGCCACGCCGCGagcggagcgg                                                             <  4:178793/140‑1 (MQ=255)
                                                                                cAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGGCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGagcggagcgg                                                             <  6:597498/140‑1 (MQ=255)
                                                                                     cGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcag                                                        <  4:547627/140‑1 (MQ=255)
                                                                                     cGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcag                                                        <  3:347428/140‑1 (MQ=255)
                                                                                     cGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACAAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCATTCAGCGCCACGCCGCGAGCGGAGCGGTTcag                                                        <  2:797427/140‑1 (MQ=255)
                                                                                       gATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcagca                                                      <  8:144474/140‑1 (MQ=255)
                                                                                       gATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcagca                                                      <  6:423883/140‑1 (MQ=255)
                                                                                       gATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcagca                                                      <  3:561849/140‑1 (MQ=255)
                                                                                             ccccTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCGG‑AGATTGGGTGTGGATTTCCCGCGCAATTTCGTAACAACGCTGGCCACTTTCATTCAGCGCCACGCCGCGAGCGGGGCGGTTCAGGAGAgttg                                                >  2:138125/1‑140 (MQ=255)
                                                                                                cTCCGGGCGCGTTTTGATTTGCGTTACGTCATCGACAACTCGCTG‑ATATTGGGTGAGGTTTTCCAGCGGAAGTTCGTTACAAAGAATGCCAAAATAAGTAACCGGCACCCCGCCAGCGGAGCGGTTAATCTGAgttgttt                                             >  2:531381/1‑139 (MQ=255)
                                                                                                 tCCGGGCGCGTTTTGATTTGCGTGACGTCATCGAGCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCTCTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGc                                            <  1:379345/140‑1 (MQ=255)
                                                                                                     ggCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGc                                        <  7:161416/140‑1 (MQ=255)
                                                                                                          gTTTTTATTTGCGTGACGTCATCGACCCGTAGCTG‑ATTTTGGGTTAGGTTTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTACTCGCCACGCCGCGCGGGGAGCGGTTCAGCAGAGTGGTTGACGGCGtttt                                   >  2:1055674/1‑140 (MQ=255)
                                                                                                          gTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTAtt                                   <  5:37479/140‑1 (MQ=255)
                                                                                                           gtttGATTTGCGTGACGTCATAGACCAGTCGCTG‑ATTTTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACGTTCAGTCAGCGCCACGCCGCGAGCGGCGCGGGTACTCAGAGTTGTTGGCAGCGTAttt                                  >  4:113047/2‑140 (MQ=255)
                                                                                                              tGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCa                               <  3:134382/140‑1 (MQ=255)
                                                                                                              tGATTTGCGTGACGGCATCGACCAGTCGCTG‑ATATGGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCa                               <  6:422098/140‑1 (MQ=255)
                                                                                                                aTTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCTGCGCCACGCCGCGAGCGGTGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAg                             <  1:370726/140‑1 (MQ=255)
                                                                                                                aTTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                                                                  <  7:72300/103‑1 (MQ=255)
                                                                                                                   tGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCCCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTAAGCAGAGTTGTTTCCAGCGTATTTTCAAGGAt                          >  7:67226/1‑140 (MQ=255)
                                                                                                                    gCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGAtt                         >  1:782524/1‑140 (MQ=255)
                                                                                                                    gCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGAtt                         >  1:1070227/1‑140 (MQ=255)
                                                                                                                    gCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGAtt                         >  2:884746/1‑140 (MQ=255)
                                                                                                                    gCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGAtt                         >  3:97968/1‑140 (MQ=255)
                                                                                                                    gCGTGACGGCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGAtt                         <  5:722326/140‑1 (MQ=255)
                                                                                                                     cGTGACGTCAGCGACCAGTCGCTG‑ATATTGGGTGAGGTTTTCCTGCGCATGGTCGTAACAACCCAGGCCACTTTCAGTCAGCGCCACGCCCCGAGCGGAGCGCTACAGCAGAGAACTAGCCAGCCTATTATCactgaatt                        >  2:963027/1‑133 (MQ=255)
                                                                                                                          cGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATc                   >  3:790492/1‑140 (MQ=255)
                                                                                                                             cATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGc                <  3:50281/140‑1 (MQ=255)
                                                                                                                             cATCGACCAGTCGCTG‑ATATTGGCTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGc                <  5:452490/140‑1 (MQ=255)
                                                                                                                               tCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCtt              >  3:1019724/1‑140 (MQ=255)
                                                                                                                                  aCCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGTCGCGGTTCCTCAGAGTTGTTGCACGCGTAATTTAAAGGATTTGTATCCGCGTGGt           >  6:705073/1‑140 (MQ=255)
                                                                                                                                  aCCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGt           <  3:767900/140‑1 (MQ=255)
                                                                                                                                  aCCAGTCGCGG‑ATATTGGGTGAGGATTTCCAGCGCAGGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGt           <  3:763052/140‑1 (MQ=255)
                                                                                                                                     cggcGCTG‑ATAATTGGTGGGGTATTCCAGCGCATGGTCGTAACAACGCTGGCCACTTTCAGTCCGCGCCACGCCGCGATGGGAGCGGTTCATCAGATTTGTTGCCAGCGGATTTTCAAGGATTTGGATCCGCTTGGTGAc        <  6:605559/137‑1 (MQ=255)
                                                                                                                                     aGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGAc        <  2:421364/140‑1 (MQ=255)
                                                                                                                                      gTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGCCa       >  7:771278/1‑140 (MQ=255)
                                                                                                                                      gTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACa       >  8:133911/1‑140 (MQ=255)
                                                                                                                                       tCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTTACaa      >  6:781172/1‑140 (MQ=255)
                                                                                                                                       tCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGCCaa      >  2:843052/1‑140 (MQ=255)
                                                                                                                                       tCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACaa      >  3:604624/1‑140 (MQ=255)
                                                                                                                                           tG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGc  <  3:1060951/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTG‑ATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGC  >  NZ_CP022686/1954816‑1955094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A