Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I74 R1
|
158 |
13.7 |
815430 |
95.7% |
780366 |
86.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,659,576 |
T→C |
F14S (TTC→TCC) |
clcB → |
voltage‑gated ClC‑type chloride channel ClcB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,659,576 | 0 | T | C | 100.0%
| 21.7
/ NA
| 8 | F14S (TTC→TCC) | clcB | voltage‑gated ClC‑type chloride channel ClcB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/3); total (5/3) |
TCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCAT > NZ_CP009273/1659517‑1659643
|
tCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCgg < 1:355764/90‑1 (MQ=255)
aTTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTcgc < 2:3185/90‑1 (MQ=255)
tGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc > 1:345576/1‑61 (MQ=255)
tGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc < 2:345576/61‑1 (MQ=255)
acaTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTc > 1:235646/1‑90 (MQ=255)
acaTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTc > 1:63010/1‑90 (MQ=255)
cTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCa > 2:220/1‑90 (MQ=255)
tATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCAt > 1:264291/1‑90 (MQ=255)
|
TCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCAT > NZ_CP009273/1659517‑1659643
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCAT > NZ_CP009273/1659517‑1659643
|
TCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCG < SRR3722212.360749/100‑1 (MQ=60)
TTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTctgtctcttatacacatct > SRR3722212.350396/1‑81 (MQ=60)
CATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTC > SRR3722212.238527/1‑100 (MQ=60)
CATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTC > SRR3722212.63776/1‑100 (MQ=60)
TCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCAT > SRR3722212.267682/1‑100 (MQ=60)
|
TCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCAT > NZ_CP009273/1659517‑1659643
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
