Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F5 I97 R1	275	44.0	1733530	96.5%	1672856	122.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	453,695	G→A	A272T (GCG→ACG)	clpX →	ATP‑dependent protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	453,695	0	G	A	100.0%	117.5 / NA	32	A272T (GCG→ACG)	clpX	ATP‑dependent protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/11); total (21/11)

GGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAAT  >  NZ_CP009273/453561‑453826

ggCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGGCTGGGTAAAAGGATTTCCCACCGGGTAGAAAACGGCGCCGGCATTGGTTTTTGcacg                                                                                                                                   >  1:85143/1‑137 (MQ=255)

                      aTTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCCGGCTGGGTAAAAAGATTTTCCACCGCGGAAAAACCGGCCCCGGAAATGGTTTTGGCACGACGGGAAAAACGGAGTCCGACa                                                                                                             >  1:221621/1‑137 (MQ=255)

                         cTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAaagc                                                                                                         >  1:81859/1‑138 (MQ=255)

                                   gATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCCTTGGTTTTTGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGc                                                                                               >  1:21526/1‑138 (MQ=255)

                                          ctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCCTTGGGTTTGGCACGACGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAgctgct                                                                                       >  1:130479/1‑139 (MQ=255)

                                              gATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg                                                                                   <  2:781829/139‑1 (MQ=255)

                                                  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGATTTTCCACCGTGTAGAAAACGGCTCCCGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGGGCTGCTGGGGCAgg                                                                               >  2:448309/1‑139 (MQ=255)

                                                  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCCCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAAGCCGACAAAGCAAGCGAAGGCGGGCTGCTGGGGCAgg                                                                               >  1:431272/1‑139 (MQ=255)

                                                  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAATGATTTCCCACCGGGTAGAAAACGGCTCCGGCGTTGGTTTTGGCACGGCGGTAAAAGCGAAGTCCGACAAAACAAGCGAAGGCGAGCTGCTGGCGCAgg                                                                               >  2:741672/1‑139 (MQ=255)

                                                  cTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGGTAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGGACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAgg                                                                               >  2:218771/1‑139 (MQ=255)

                                                   tGTTTATTTGTGGCGGTGCGGTTGCCGGTCTGGATAAAGTGATTTTCCACCGTGTAGAAACCGGCTCCGGCATTGGGTTTGGCACGACGGGAAAAGCGAAGGCCGACAAAGCAAGCGAAGGGGAGCTGCTGGCGCAAGt                                                                              >  2:402268/1‑139 (MQ=255)

                                                   tGTTTATTTGTGGCGGTGCGGTTGCCGGTCTGGATAAAGTGATTTTCCACCGTGTAGAAAACGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGt                                                                              >  2:291828/1‑139 (MQ=255)

                                                             tgGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGa                                                                     <  2:248029/138‑1 (MQ=255)

                                                               gCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAg                                                                   <  2:794058/138‑1 (MQ=255)

                                                                                cTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTc                                                 >  2:593720/1‑139 (MQ=255)

                                                                                   gATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCt                                                <  1:291828/137‑1 (MQ=255)

                                                                                   gATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTa                                              <  1:218547/139‑1 (MQ=255)

                                                                                        aGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt                                         >  2:179911/1‑139 (MQ=255)

                                                                                             tttCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGCCGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGtt                                    >  2:62618/1‑139 (MQ=255)

                                                                                             tttCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGtt                                    >  1:75742/1‑139 (MQ=255)

                                                                                                    ccGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAgctg                                                                                         <  1:297262/79‑1 (MQ=255)

                                                                                                    ccGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAgctg                                                                                         >  2:297262/1‑79 (MQ=255)

                                                                                                      gtgtAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                            >  1:731513/1‑106 (MQ=255)

                                                                                                      gtgtAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                            <  2:731513/106‑1 (MQ=255)

                                                                                                      gtgtAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGgtcg                           >  2:136628/1‑139 (MQ=255)

                                                                                                         tAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCt                        >  1:131507/1‑139 (MQ=255)

                                                                                                                cGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTAt                                 <  2:29668/123‑1 (MQ=255)

                                                                                                                cGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTAt                                 >  1:29668/1‑123 (MQ=255)

                                                                                                                 ggCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTg                >  1:694301/1‑139 (MQ=255)

                                                                                                                   cTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                            <  2:445834/93‑1 (MQ=255)

                                                                                                                   cTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                            >  1:445834/1‑93 (MQ=255)

                                                                                                                        gCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc         <  1:218771/139‑1 (MQ=255)

                                                                                                                               ttttGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtgaat  <  2:75742/139‑1 (MQ=255)

GGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAAT  >  NZ_CP009273/453561‑453826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCG  >  NZ_CP009273/453545‑453836

gagattcgccttagtctcgtgggctcggagatgtgtataagagacagGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCA                                                                                                                                               <  SRR3722120.269524/104‑1 (MQ=60)

     ACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGGCTGGGTAAAAGGATTTCCCACCGGGTAGAAAACGGCGCCGGCATTGGTTTTTGCACGA                                                                                                                                            >  SRR3722120.86237/1‑149 (MQ=60)

         CAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAA                                                                                                                                      <  SRR3722120.723956/151‑1 (MQ=60)

                   GTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTC                                                                                                                            <  SRR3722120.27068/151‑1 (MQ=60)

                           CCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCCGGCTGGGTAAAAAGATTTTCCACCGCGGAAAAACCGGCCCCGGAAATGGTTTTGGCACGACGGGAAAAACGGAGTCCGACAA                                                                                                                      >  SRR3722120.224722/1‑149 (MQ=60)

                              CAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCA                                                                                                                  >  SRR3722120.82918/1‑150 (MQ=60)

                                        TCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCCTTGGTTTTTGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCG                                                                                                        >  SRR3722120.21768/1‑150 (MQ=60)

                                               GGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCCTTGGGTTTGGCACGACGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTG                                                                                                >  SRR3722120.132240/1‑151 (MQ=60)

                                                       CCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCCCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAAGCCGACAAAGCAAGCGAAGGCGGGCTGCTGGGGCAGGG                                                                                        >  SRR3722120.437599/1‑151 (MQ=60)

                                                        acgagattcgccttagtctcgtgggctcggagatgtgtataagagacagGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGT                                                                                        <  SRR3722120.301512/101‑1 (MQ=60)

                                                                                                  GGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTT                                             >  SRR3722120.76734/1‑151 (MQ=60)

                                                                                                  GGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTT                                             <  SRR3722120.221608/151‑1 (MQ=60)

                                                                                                  GGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGT                                               <  SRR3722120.296014/149‑1 (MQ=60)

                                                                                                           GATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCctgtctcttatacacatctccg                                     >  SRR3722120.741417/1‑128 (MQ=60)

                                                                                                              TTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTG                                 >  SRR3722120.133280/1‑151 (MQ=60)

                                                                                                                     CGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCtgtct                          >  SRR3722120.30009/1‑146 (MQ=60)

                                                                                                                      GTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGT                         >  SRR3722120.703781/1‑151 (MQ=60)

                                                                                                                        GTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCctgtctcttatacacatctccgagcccacgagact                        >  SRR3722120.452343/1‑115 (MQ=60)

                                                                                                                                       GGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAAC        <  SRR3722120.221834/151‑1 (MQ=60)

                                                                                                                                             GGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtcttctgcttg   >  SRR3722120.535647/1‑84 (MQ=60)

                                                                                                                                             GGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtcttctg  >  SRR3722120.445284/1‑89 (MQ=60)

GTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCG  >  NZ_CP009273/453545‑453836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`