Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F5 I176 R1	180	42.9	1710034	96.5%	1650182	119.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	798,635	T→C	G431G (GGT→GGC)	ybhI →	anion permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	798,635	0	T	C	100.0%	153.8 / NA	45	G431G (GGT→GGC)	ybhI	anion permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/22); total (23/22)

CTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAA  >  NZ_CP009273/798500‑798766

cTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCg                                                                                                                                  <  2:277842/139‑1 (MQ=255)

   tatCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCCGt                                                                                                                                <  1:629608/138‑1 (MQ=255)

        ttGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCtt                                                                                                                          >  1:512481/1‑139 (MQ=255)

         tGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGCCATCttt                                                                                                                         >  2:575381/1‑139 (MQ=255)

           cTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                <  2:43396/130‑1 (MQ=255)

           cTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                >  1:43396/1‑130 (MQ=255)

                  aCCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCAGCTTTGGCGTGGGt                                                                                                                >  2:182622/1‑139 (MQ=255)

                         tttGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACg                                                                                                         <  2:12274/139‑1 (MQ=255)

                            gCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGata                                                                                                      >  2:848713/1‑139 (MQ=255)

                                                cGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGtggttggt                                                                                   <  2:512481/138‑1 (MQ=255)

                                                    gcACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTg                                                                              <  1:848713/139‑1 (MQ=255)

                                                    gcACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTg                                                                              <  1:150952/139‑1 (MQ=255)

                                                          ttAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCg                                                                                                                     <  1:570138/94‑1 (MQ=255)

                                                          ttAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCg                                                                                                                     >  2:570138/1‑94 (MQ=255)

                                                             aTGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTg                                                                      <  1:72127/138‑1 (MQ=255)

                                                             aTGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGa                                                                     <  1:610342/139‑1 (MQ=255)

                                                                  aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCCTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCCTCTTTGGGGGGGGGTATTACGATATTAAATCCTGGTGGTTGGTCGGTGCGGGACTGCAGata                                                                >  2:72127/1‑139 (MQ=255)

                                                                  aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                >  1:5389/1‑75 (MQ=255)

                                                                  aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                <  2:5389/75‑1 (MQ=255)

                                                                  aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                >  1:57208/1‑75 (MQ=255)

                                                                  aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                <  2:57208/75‑1 (MQ=255)

                                                                  aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGata                                                                >  2:126492/1‑139 (MQ=255)

                                                                       cgcTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAAc                                                           >  2:436128/1‑139 (MQ=255)

                                                                              aCTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTg                                                    <  2:443684/139‑1 (MQ=255)

                                                                                 gttgttCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCg                                                                                                                     <  2:561295/71‑1 (MQ=255)

                                                                                 gttgttCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCg                                                                                                                     >  1:561295/1‑71 (MQ=255)

                                                                                  ttgttCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGGGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTg                                                 >  1:697099/1‑138 (MQ=255)

                                                                                                   tGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCgtg                               <  1:148557/139‑1 (MQ=255)

                                                                                                   tGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCgtg                               <  1:558354/139‑1 (MQ=255)

                                                                                                          aTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCt                                      >  1:135665/1‑125 (MQ=255)

                                                                                                          aTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCt                                      <  2:135665/125‑1 (MQ=255)

                                                                                                          aTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCgtg                               <  2:300632/132‑1 (MQ=255)

                                                                                                          aTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCgtg                               >  1:300632/1‑132 (MQ=255)

                                                                                                                      tATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCAccc                                       >  2:212956/1‑112 (MQ=255)

                                                                                                                      tATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCAccc                                       <  1:212956/112‑1 (MQ=255)

                                                                                                                      tATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGa            >  1:208742/1‑139 (MQ=255)

                                                                                                                              cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTg                                                                              >  2:438609/1‑65 (MQ=255)

                                                                                                                              cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTg                                                                              <  1:438609/65‑1 (MQ=255)

                                                                                                                              cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTcc                                                      <  1:196372/89‑1 (MQ=255)

                                                                                                                              cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTcc                                                      >  2:196372/1‑89 (MQ=255)

                                                                                                                              cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTg     >  2:140266/1‑138 (MQ=255)

                                                                                                                              cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTg     >  1:28528/1‑138 (MQ=255)

                                                                                                                                cggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGa                                                                     >  1:715261/1‑72 (MQ=255)

                                                                                                                                cggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGa                                                                     <  2:715261/72‑1 (MQ=255)

                                                                                                                                cggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGGGGTGGAATATGCTGATCGGCTGGaa  >  1:447655/1‑139 (MQ=255)

CTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAA  >  NZ_CP009273/798500‑798766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTTCCGGTAGTGCCTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAAC  >  NZ_CP009273/798486‑798767

GCTTCCGGTAGTGCCTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCC                                                                                                                                     <  SRR3722130.107273/151‑1 (MQ=60)

           TGCCTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTT                                                                                                                          >  SRR3722130.520356/1‑151 (MQ=60)

            gtgtataagagacagATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTT                                                                                                                          <  SRR3722130.557760/135‑1 (MQ=60)

              CTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGC                                                                                                                       >  SRR3722130.43990/1‑151 (MQ=60)

                ATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCCGTCATCTTTGGCG                                                                                                                      <  SRR3722130.639035/150‑1 (MQ=60)

                          agtctcgtgggctcggagatgtgtataagagacagCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAAC                                                                                                           <  SRR3722130.578757/116‑1 (MQ=60)

                                                                 agcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACG                                                                    <  SRR3722130.445496/87‑1 (MQ=60)

                                                                 CGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACG                                                                    <  SRR3722130.860971/151‑1 (MQ=60)

                                                                 CGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACG                                                                    <  SRR3722130.153191/151‑1 (MQ=60)

                                                                     CCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgcc                                                                >  SRR3722130.57994/1‑97 (MQ=60)

                                                                     CCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGctgtctcttatacacatctccgagcccacgagactaaggcgaatatcgtatgcc                                                                >  SRR3722130.5464/1‑97 (MQ=60)

                                                                          AATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACC                                                           <  SRR3722130.619484/151‑1 (MQ=60)

                                                                          AATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAAC                                                            <  SRR3722130.73151/150‑1 (MQ=60)

                                                                                    GCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtct                                                 >  SRR3722130.569787/1‑93 (MQ=60)

                                                                                     CGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGGGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGC                                                 >  SRR3722130.707411/1‑150 (MQ=60)

                                                                                         gccttagtctcgtgggctcggagatgtgtataagagacagCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATC                                            <  SRR3722130.199357/111‑1 (MQ=60)

                                                                                                        atgtgtataagagacagTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGG                             <  SRR3722130.216177/134‑1 (MQ=60)

                                                                                                             CCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTctgt                        >  SRR3722130.137678/1‑147 (MQ=60)

                                                                                                             CCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTG                        >  SRR3722130.305293/1‑151 (MQ=60)

                                                                                                                ATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAA                     <  SRR3722130.566805/151‑1 (MQ=60)

                                                                                                                ATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAA                     <  SRR3722130.150755/151‑1 (MQ=60)

                                                                                                                         TGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGAT            >  SRR3722130.211904/1‑151 (MQ=60)

                                                                                                                                 CACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGG     >  SRR3722130.28901/1‑150 (MQ=60)

                                                                                                                                   CTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCctgtctcttatacacatctccgagcccacgagactaaggcgaatatcgtatgccgtc  >  SRR3722130.725823/1‑94 (MQ=60)

                                                                                                                                   CTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGGGGTGGAATATGCTGATCGGCTGGAAC  >  SRR3722130.454663/1‑151 (MQ=60)

GCTTCCGGTAGTGCCTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAAC  >  NZ_CP009273/798486‑798767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`