Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F5 I175 R1	328	64.7	2582366	96.2%	2484236	120.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	797,386	T→C	I15T (ATC→ACC)	ybhI →	anion permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	797,386	0	T	C	100.0%	149.3 / NA	42	I15T (ATC→ACC)	ybhI	anion permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/21); total (21/21)

AAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGT  >  NZ_CP009273/797251‑797514

aaGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGAcccc                                                                                                                               <  2:1284603/139‑1 (MQ=255)

aaGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGAcccc                                                                                                                               <  2:764334/139‑1 (MQ=255)

  gTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCAt                                                                                                                             <  1:94715/139‑1 (MQ=255)

      aTCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGta                                                                                                                          <  2:604762/138‑1 (MQ=255)

          tCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTattatt                                                                                                                     >  1:616100/1‑139 (MQ=255)

          tCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTattatt                                                                                                                     >  1:288623/1‑139 (MQ=255)

                     aTTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGcc                                                                                                          <  1:1018961/139‑1 (MQ=255)

                         gTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGc                                                                                                       <  1:687568/138‑1 (MQ=255)

                             tCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCgg                                                                                                  <  1:1233774/139‑1 (MQ=255)

                                      ggACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTaa                                                                                         <  1:1043146/139‑1 (MQ=255)

                                       gACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAg                                                                                        >  1:781183/1‑139 (MQ=255)

                                       gACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAg                                                                                        >  1:1023336/1‑139 (MQ=255)

                                           atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa                                                                                    >  2:449726/1‑139 (MQ=255)

                                           atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa                                                                                    <  2:781183/139‑1 (MQ=255)

                                           atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa                                                                                    >  1:1212964/1‑139 (MQ=255)

                                            tcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAAc                                                                                   >  1:709379/1‑139 (MQ=255)

                                                        ttCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGc                                                                       >  1:177901/1‑139 (MQ=255)

                                                             tATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCttt                                                                    <  1:747089/137‑1 (MQ=255)

                                                                  gTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGtattat                                                                                                                      <  1:955738/82‑1 (MQ=255)

                                                                  gTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGtattat                                                                                                                      >  2:955738/1‑82 (MQ=255)

                                                                     aTACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGttt                                                                                                                >  2:291524/1‑85 (MQ=255)

                                                                     aTACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGttt                                                                                                                <  1:291524/85‑1 (MQ=255)

                                                                     aTACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTa                                                          >  2:1277817/1‑139 (MQ=255)

                                                                           aaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggc                                                    >  1:646983/1‑139 (MQ=255)

                                                                             agaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGAcccc                                                                                                                               >  1:378262/1‑62 (MQ=255)

                                                                             agaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGAcccc                                                                                                                               <  2:378262/62‑1 (MQ=255)

                                                                              gaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcggc                                                 <  2:709379/139‑1 (MQ=255)

                                                                              gaaCAGGATTTTAAACGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcggc                                                 <  1:318928/139‑1 (MQ=255)

                                                                                aCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcggc                                                 >  1:847590/1‑137 (MQ=255)

                                                                                aCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcggc                                                 <  2:847590/137‑1 (MQ=255)

                                                                                aCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCa                                               >  2:451282/1‑139 (MQ=255)

                                                                                          aaaTGAATAAGAAATCGTTATGGAAGCTAAGTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGc                                      <  2:905467/138‑1 (MQ=255)

                                                                                           aaTGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTg                                    <  2:898367/139‑1 (MQ=255)

                                                                                              gAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGt                                  >  2:697101/1‑138 (MQ=255)

                                                                                                         cGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCtttt                                                                   <  1:464154/94‑1 (MQ=255)

                                                                                                         cGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCtttt                                                                   >  2:464154/1‑94 (MQ=255)

                                                                                                           ttATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTcc                    >  1:773804/1‑139 (MQ=255)

                                                                                                           ttATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTcc                    >  2:400283/1‑139 (MQ=255)

                                                                                                              tGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCg                  >  2:1152137/1‑138 (MQ=255)

                                                                                                                gAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGa                <  2:1212964/138‑1 (MQ=255)

                                                                                                                gAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGa                <  2:1032005/138‑1 (MQ=255)

                                                                                                                 aaGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAAc              <  2:1023336/139‑1 (MQ=255)

                                                                                                                    cTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTg           <  2:254393/139‑1 (MQ=255)

                                                                                                                           tGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcg                                                   >  2:1253560/1‑92 (MQ=255)

                                                                                                                           tGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcg                                                   <  1:1253560/92‑1 (MQ=255)

                                                                                                                             atatTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGt  >  1:517163/1‑139 (MQ=255)

AAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGT  >  NZ_CP009273/797251‑797514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGTTAATTGCCGTTGCTGCCTCA  >  NZ_CP009273/797245‑797534

ggttttttttttttcaagcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTA                                                                                                                                              <  SRR3722129.922585/70‑1 (MQ=60)

CCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTAT                                                                                                                                             <  SRR3722129.997656/151‑1 (MQ=60)

CCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTAT                                                                                                                                             <  SRR3722129.993291/151‑1 (MQ=60)

     GAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTG                                                                                                                                        >  SRR3722129.625264/1‑151 (MQ=60)

     GAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTG                                                                                                                                        >  SRR3722129.292768/1‑151 (MQ=60)

       AGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGT                                                                                                                                      <  SRR3722129.95999/151‑1 (MQ=60)

              gagattcgccttagtctcgtgggctcggagatgtgtataagagacagCTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGC                                                                                                                               <  SRR3722129.969238/104‑1 (MQ=60)

                    attcgccttagtctcgtgggctcggagatgtgtataagagacagCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTC                                                                                                                         <  SRR3722129.295704/107‑1 (MQ=60)

                          AATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAG                                                                                                                   <  SRR3722129.1033340/151‑1 (MQ=60)

                              CGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGAT                                                                                                                <  SRR3722129.697700/150‑1 (MQ=60)

                                  GTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGC                                                                                                           >  SRR3722129.1037763/1‑151 (MQ=60)

                                  GTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGC                                                                                                           >  SRR3722129.792520/1‑151 (MQ=60)

                                  GTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGC                                                                                                           <  SRR3722129.1250915/151‑1 (MQ=60)

                                      GGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAAC                                                                                                       >  SRR3722129.1229868/1‑151 (MQ=60)

                                       GATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACT                                                                                                      >  SRR3722129.719811/1‑151 (MQ=60)

                                           AGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCG                                                                                                  <  SRR3722129.1057797/151‑1 (MQ=60)

                                                   CAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCT                                                                                          >  SRR3722129.180342/1‑151 (MQ=60)

                                                                 agtctcgtgggctcggagatgtgtataagagacagGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACC                                                                            <  SRR3722129.470904/116‑1 (MQ=60)

                                                                  GTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTAC                                                                             <  SRR3722129.758003/149‑1 (MQ=60)

                                                                      GAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCG                                                                       >  SRR3722129.656628/1‑151 (MQ=60)

                                                                        GTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtcttctgcttg                                                                      >  SRR3722129.383789/1‑84 (MQ=60)

                                                                           ATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCAT                                                                  >  SRR3722129.859714/1‑151 (MQ=60)

                                                                                  tagtctcgtgggctcggagatgtgtataagagacaGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGG                                                            <  SRR3722129.1270998/115‑1 (MQ=60)

                                                                                   AGAACAGGATTTTAAACGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGC                                                          <  SRR3722129.323481/151‑1 (MQ=60)

                                                                                                      ATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCC                                       >  SRR3722129.785032/1‑151 (MQ=60)

                                                                                                                        AGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGTT                     >  SRR3722129.524720/1‑151 (MQ=60)

                                                                                                                                  GATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGTTAATTGCCGTT           >  SRR3722129.577586/1‑151 (MQ=60)

                                                                                                                                            ACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGTTAATTGCCGTTGCTGCCTCA  >  SRR3722129.746380/1‑150 (MQ=60)

CCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGTTAATTGCCGTTGCTGCCTCA  >  NZ_CP009273/797245‑797534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`