Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F5 I175 R1	328	64.7	2582366	96.2%	2484236	120.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	937,543	A→C	T377P (ACC→CCC)	dmsA →	dimethylsulfoxide reductase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	937,543	0	A	C	88.0%	149.7 / 13.2	50	T377P (ACC→CCC)	dmsA	dimethylsulfoxide reductase subunit A
Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (16/28); total (19/31)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01

CGCCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTG  >  NZ_CP009273/937406‑937678

cgcCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTAc                                                                                                                                        >  2:1257780/1‑139 (MQ=255)

cgcCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTAc                                                                                                                                        <  2:1204563/139‑1 (MQ=255)

         aTGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGc                                                                                                                               <  2:1176465/139‑1 (MQ=255)

              cACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTa                                                                                                                          <  1:1283840/139‑1 (MQ=255)

                   taAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGc                                                                                                                     <  1:1192519/139‑1 (MQ=255)

                   taAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGc                                                                                                                     <  1:1257780/139‑1 (MQ=255)

                     aaGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCa                                                                                                                   <  1:1079506/139‑1 (MQ=255)

                     aaGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCa                                                                                                                   <  1:385507/139‑1 (MQ=255)

                           atatTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATg                                                                                                             >  2:403465/1‑139 (MQ=255)

                                  gggTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCg                                                                                                      >  1:665540/1‑139 (MQ=255)

                                  gggTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCAAAACCGGCGTTTATCAGCCAGGGATGGGGCCCg                                                                                                      >  2:1129614/1‑139 (MQ=255)

                                   ggTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCg                                                                                                      >  2:33404/1‑138 (MQ=255)

                                   ggTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATAAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCg                                                                                                      >  2:1061658/1‑138 (MQ=255)

                                       aaGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCg                                                                                                 >  2:372872/1‑139 (MQ=255)

                                        aGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTAt                                                                                                                         >  1:590797/1‑114 (MQ=255)

                                        aGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTAt                                                                                                                         <  2:590797/114‑1 (MQ=255)

                                            ccAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACg                                                                                            <  2:322515/139‑1 (MQ=255)

                                            ccAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACg                                                                                            <  2:915268/139‑1 (MQ=255)

                                            ccAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACg                                                                                            <  1:722651/139‑1 (MQ=255)

                                             cAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGc                                                                                           >  2:414320/1‑139 (MQ=255)

                                                    cGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGt                                                                                    >  1:988736/1‑139 (MQ=255)

                                                            aaaCGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGc                                                                            <  2:525831/139‑1 (MQ=255)

                                                                  cGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCg                                                                      <  2:201986/139‑1 (MQ=255)

                                                                  cGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCg                                                                      >  2:133886/1‑139 (MQ=255)

                                                                   ggAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGt                                                                     >  2:512027/1‑139 (MQ=255)

                                                                    gAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTg                                                                    <  2:307002/139‑1 (MQ=255)

                                                                          gCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTAtct                                                              <  2:143567/139‑1 (MQ=255)

                                                                              cGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGAt                                                          >  2:496185/1‑139 (MQ=255)

                                                                                   aTCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTgg                                                     <  1:237048/139‑1 (MQ=255)

                                                                                             ttCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCTAAAc                                                                                                                                   <  2:273645/51‑1 (MQ=255)

                                                                                             ttCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAAc                                                                                                                                   >  1:273645/1‑51 (MQ=255)

                                                                                             ttCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAAc                                                                         <  2:297451/109‑1 (MQ=255)

                                                                                             ttCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAAc                                                                         >  1:297451/1‑109 (MQ=255)

                                                                                               ccGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCa                                                                                                                       <  2:782986/61‑1 (MQ=255)

                                                                                               ccGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCa                                                                                                                       >  1:782986/1‑61 (MQ=255)

                                                                                                 ggCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGt                                       <  2:665540/139‑1 (MQ=255)

                                                                                                  gCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGCCCGGt                                       >  2:1144519/1‑138 (MQ=255)

                                                                                                     ggcAAAATCATCAAAGTGGCTCGTGATGTCGGTAGTCCCAAACCGGCGTTTATCAGCCAGTGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCGGGCTATTCTGACCTGTAACg                                   <  2:414139/137‑1 (MQ=255)

                                                                                                      aCAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGt                                  <  1:401868/139‑1 (MQ=255)

                                                                                                       cAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCTGTAACGtt                                 <  2:176681/139‑1 (MQ=255)

                                                                                                       cAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGtt                                 <  2:770493/139‑1 (MQ=255)

                                                                                                       cAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGtt                                 <  1:634580/139‑1 (MQ=255)

                                                                                                                 aaaTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACg                       <  2:222685/139‑1 (MQ=255)

                                                                                                                  aaTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGc                                                                                                                     >  1:1087313/1‑44 (MQ=255)

                                                                                                                  aaTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGc                                                                                                                     <  2:1087313/44‑1 (MQ=255)

                                                                                                                   aTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGa                     <  1:512027/139‑1 (MQ=255)

                                                                                                                   aTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGa                     <  1:861551/139‑1 (MQ=255)

                                                                                                                             gcaaTCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTg                                                                                   >  1:302090/3‑67 (MQ=255)

                                                                                                                             gAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTg                                                                                   <  2:302090/67‑1 (MQ=255)

                                                                                                                               aaTCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAgcggc         <  1:1144519/139‑1 (MQ=255)

                                                                                                                                      agtcCCAAACCGGCGGTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTg  <  2:490995/139‑1 (MQ=255)

CGCCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTG  >  NZ_CP009273/937406‑937678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCAGCCAGTGCGCCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTGAAGGTTCATACAG  >  NZ_CP009273/937395‑937691

GCCAGCCAGTGCGCCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCC                                                                                                                                                    <  SRR3722129.782445/151‑1 (MQ=60)

     acgagattcgccttagtctcgtgggctcggagatgtgtataagagacaGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAAC                                                                                                                                                <  SRR3722129.1067621/102‑1 (MQ=60)

                        CCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGA                                                                                                                            <  SRR3722129.1301637/151‑1 (MQ=60)

                             ATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGG                                                                                                                       <  SRR3722129.1209115/151‑1 (MQ=60)

                             ATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGG                                                                                                                       <  SRR3722129.1275274/151‑1 (MQ=60)

                               AAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCC                                                                                                                     <  SRR3722129.1094627/151‑1 (MQ=60)

                               AAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCC                                                                                                                     <  SRR3722129.391142/151‑1 (MQ=60)

                                  GCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGC                                                                                                                  >  SRR3722129.675397/1‑151 (MQ=60)

                                        ATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATctgtctcttatacac                                                                                                            >  SRR3722129.599531/1‑136 (MQ=60)

                                                    GGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTG                                                                                                >  SRR3722129.1002707/1‑151 (MQ=60)

                                                      GCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAA                                                                                              <  SRR3722129.733243/151‑1 (MQ=60)

                                                                                             AATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCctgtctcttatgcacatctccgagcccacgagactaaggcgaatctcgtatgccgtcttctgcttgaaaaaaaaaa                                                         >  SRR3722129.277531/1‑73 (MQ=60)

                                                                                             AATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCtgtctcttatacacatctc                                                       >  SRR3722129.301706/1‑132 (MQ=60)

                                                                                             AATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACC                                                       <  SRR3722129.240421/151‑1 (MQ=60)

                                                                                               TCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtcttctgcttga                                                      >  SRR3722129.794342/1‑83 (MQ=60)

                                                                                                                GACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACG                                    <  SRR3722129.407702/151‑1 (MQ=60)

                                                                                                                 ACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGG                                   <  SRR3722129.644018/151‑1 (MQ=60)

                                                                                                                  CAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCtgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtcttctgcttgcaaaaaaaaaatcacaccg                                  >  SRR3722129.1102530/1‑67 (MQ=60)

                                                                                                                             AATTGGCTCGTGCAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGGCACGCTAACGGTGAAATCGCAACCCtgtctcttatacacatctccgagcccacgagactaaggcgaatatcgtgtgccgtcttctg                       >  SRR3722129.306410/1‑90 (MQ=60)

                                                                                                                             AATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGG                       <  SRR3722129.519526/151‑1 (MQ=60)

                                                                                                                             AATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGG                       <  SRR3722129.873827/151‑1 (MQ=60)

                                                                                                                                         AAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTGAAGG           <  SRR3722129.1160426/151‑1 (MQ=60)

                                                                                                                                          AATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTGAAGGT          >  SRR3722129.523478/1‑151 (MQ=60)

                                                                                                                                             CGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTGAAGGTTC        >  SRR3722129.833642/1‑150 (MQ=60)

                                                                                                                                                  GTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtcttctgcttga   >  SRR3722129.1071936/1‑86 (MQ=60)

                                                                                                                                                  GTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGctgtctcttatacacatctccgagcccacgagac  >  SRR3722129.1192542/1‑117 (MQ=60)

                                                                                                                                                  GTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTGAAGGTctgtctct  >  SRR3722129.1307942/1‑143 (MQ=60)

                                                                                                                                                  GTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTGAAGGTTCATAC    >  SRR3722129.567179/1‑149 (MQ=60)

GCCAGCCAGTGCGCCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTGAAGGTTCATACAG  >  NZ_CP009273/937395‑937691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`