Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F86 I0 R1	593	297.4	9515568	94.4%	8982696	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	320,920	G→A	93.5%	S52S (AGC→AGT)	ykgH ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	320,920	0	G	A	93.5%	460.3 / 16.9	139	S52S (AGC→AGT)	ykgH	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (3/6); new base A (59/71); total (62/77)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

Only 100 of 139 total aligned reads displayed.

TTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTG  >  CP009273/320775‑321066

 ttCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTa                                                                                                                                               <  1:4092659/150‑1 (MQ=255)

  tctcTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTaa                                                                                                                                              <  1:1513344/150‑1 (MQ=255)

   ctctGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAATCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTaaa                                                                                                                                             <  2:209/150‑1 (MQ=255)

    tctGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAAt                                                                                                                                            >  2:3686627/1‑150 (MQ=255)

    tctGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCAGTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAAt                                                                                                                                            >  2:3686599/1‑150 (MQ=255)

      tGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCa                                                                                                                                          <  2:637197/150‑1 (MQ=255)

      tGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCa                                                                                                                                          >  1:3576777/1‑150 (MQ=255)

      tGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCa                                                                                                                                          <  1:2752498/150‑1 (MQ=255)

       gTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCAc                                                                                                                                         <  1:645663/150‑1 (MQ=255)

          cTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACttt                                                                                                                                      <  1:639891/150‑1 (MQ=255)

            tGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTta                                                                                                                                    >  2:2572473/1‑150 (MQ=255)

            tGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTta                                                                                                                                    >  2:3051376/1‑150 (MQ=255)

                aTCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATAtt                                                                                                                                >  2:1402741/1‑150 (MQ=255)

                aTCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATAtt                                                                                                                                >  1:1659019/1‑150 (MQ=255)

                aTCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATAtt                                                                                                                                >  2:1061039/1‑150 (MQ=255)

                aTCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATAtt                                                                                                                                >  2:1061053/1‑150 (MQ=255)

                aTCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATAtt                                                                                                                                >  2:1061062/1‑150 (MQ=255)

                 tCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTg                                                                                                                               >  2:3788449/1‑150 (MQ=255)

                 tCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTg                                                                                                                               <  2:1739595/150‑1 (MQ=255)

                  cTGGGCTGTTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGt                                                                                                                              <  1:4385680/150‑1 (MQ=255)

                   tGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTc                                                                                                                             <  1:1371753/150‑1 (MQ=255)

                      gCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCttt                                                                                                                          <  2:1898164/150‑1 (MQ=255)

                        tGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTAt                                                                                                                        <  2:4476557/150‑1 (MQ=255)

                          gtgtGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTg                                                                                                                      <  2:305109/150‑1 (MQ=255)

                                aaaaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAc                                                                                                                <  1:3216417/150‑1 (MQ=255)

                                aaaaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAc                                                                                                                <  1:3215722/150‑1 (MQ=255)

                                   aaTTTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACATGCAAAATTATTATTTCTATCAAAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTATTTATTGATATACAGa                                                                                                             <  2:2602436/150‑1 (MQ=255)

                                   aaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGa                                                                                                             >  2:191846/1‑150 (MQ=255)

                                   aaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGa                                                                                                             >  1:2837701/1‑150 (MQ=255)

                                   aaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGa                                                                                                             >  1:1566445/1‑150 (MQ=255)

                                     tGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATACTGTAAATCACTTTTATATTTTCTTTATTTATATACAGaaa                                                                                                           >  1:1323804/1‑150 (MQ=255)

                                     tGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGaaa                                                                                                           >  1:2741132/1‑150 (MQ=255)

                                     tGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGaaa                                                                                                           >  1:372954/1‑150 (MQ=255)

                                     tGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGaaa                                                                                                           >  1:2364050/1‑150 (MQ=255)

                                     tGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGaaa                                                                                                           <  2:4176955/150‑1 (MQ=255)

                                       tagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaaca                                                                                                         >  2:1753824/1‑150 (MQ=255)

                                                  tGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATa                                                                                              <  2:4741056/150‑1 (MQ=255)

                                                   gAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTTTTATTGATATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAg                                                                                             <  2:63342/150‑1 (MQ=255)

                                                   gAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAg                                                                                             >  2:3906536/1‑150 (MQ=255)

                                                      tAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATCCCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCca                                                                                          <  2:2767092/150‑1 (MQ=255)

                                                          aTCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcataca                                                                                      >  2:1711763/1‑150 (MQ=255)

                                                          aTCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcataca                                                                                      <  2:2824900/150‑1 (MQ=255)

                                                          aTCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcataca                                                                                      >  2:985309/1‑150 (MQ=255)

                                                            cTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGTTATACAGAAACAACAGCACCATAGCcatacata                                                                                    >  1:1776623/1‑150 (MQ=255)

                                                            cTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcatacata                                                                                    <  1:2052538/150‑1 (MQ=255)

                                                              tCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAtata                                                                                  <  2:636825/150‑1 (MQ=255)

                                                               cAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAg                                                                                 >  2:3387601/1‑150 (MQ=255)

                                                                aGTCATTGTAGTCTGACAGGAAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGc                                                                                <  1:2564883/150‑1 (MQ=255)

                                                                  tCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCa                                                                              <  1:1268404/150‑1 (MQ=255)

                                                                   cATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCat                                                                             <  2:372954/150‑1 (MQ=255)

                                                                   cATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCat                                                                             <  2:2364050/150‑1 (MQ=255)

                                                                   cATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCat                                                                             <  2:1272666/150‑1 (MQ=255)

                                                                    aTTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCata                                                                            <  1:1111408/150‑1 (MQ=255)

                                                                       gTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataata                                                                         <  2:1134830/150‑1 (MQ=255)

                                                                           tCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTTTGTTTTTTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATaaaaa                                                                     >  1:1264078/1‑150 (MQ=255)

                                                                           tCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATaaaaa                                                                     >  1:557479/1‑150 (MQ=255)

                                                                           tCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGAAAGTAAATCACTTTTATATTGTCTTTATTGTTATACAGAAACAACAGCACCATAGCCATACAAATATCCATAATaaaaa                                                                     >  1:1263540/1‑150 (MQ=255)

                                                                             tGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAgg                                                                   <  2:4404085/150‑1 (MQ=255)

                                                                               aCAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGAt                                                                 <  1:4429506/150‑1 (MQ=255)

                                                                                 aGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATaa                                                               >  1:3930704/1‑150 (MQ=255)

                                                                                  ggaaaaaTTATTATTGATATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAac                                                              <  1:2545630/147‑1 (MQ=255)

                                                                                   gCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAaca                                                             <  2:2839670/150‑1 (MQ=255)

                                                                                    cAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAacac                                                            <  1:1402741/150‑1 (MQ=255)

                                                                                    cAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAacac                                                            <  1:3561347/150‑1 (MQ=255)

                                                                                        attattattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAAt                                                        <  1:3051376/150‑1 (MQ=255)

                                                                                          tattattGCTATCACCGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCt                                                      <  1:4109442/150‑1 (MQ=255)

                                                                                           attattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCtt                                                     <  2:3266141/150‑1 (MQ=255)

                                                                                            ttattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttt                                                    <  2:3628888/150‑1 (MQ=255)

                                                                                              attGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttttt                                                  >  1:4305624/1‑150 (MQ=255)

                                                                                               ttGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTttc                                                 <  1:3091153/150‑1 (MQ=255)

                                                                                               ttGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTttc                                                 >  2:3661559/1‑150 (MQ=255)

                                                                                                   tATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACAAGAATCTTTttcttaa                                             >  2:3303384/1‑148 (MQ=255)

                                                                                                       acaGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGata                                         <  1:808425/150‑1 (MQ=255)

                                                                                                            tAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaata                                    <  1:1363194/150‑1 (MQ=255)

                                                                                                             aaCAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaataa                                   <  1:2043294/150‑1 (MQ=255)

                                                                                                              aCAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaataaa                                  <  2:2708180/150‑1 (MQ=255)

                                                                                                                aaGTATACCTGGCGTTGTCTGGTTTATTTTTTTACTTTAAATAACTTTTATATTGTCTTTATTGATATAAATAAACAACAGCACCATAGCCATAAATATAGGCATAATAAAAAGGATAACAAGAATCTTTTTATTAAGATaaaataaaat                                >  2:803543/1‑150 (MQ=255)

                                                                                                                aaGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATaaaataaaat                                >  2:4297459/1‑150 (MQ=255)

                                                                                                                aaGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATaaaataaaat                                >  2:3810179/1‑150 (MQ=255)

                                                                                                                     taCCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAAAGCCATACATATATCCATAATAAAAAGGATAACACGAATCTTTTTCTTAAGATAAAATAAAAACTaaa                           >  1:270800/1‑150 (MQ=255)

                                                                                                                       ccTGGCGTTGTCTTGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATc                         <  2:4386569/150‑1 (MQ=255)

                                                                                                                         tGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAg                       >  1:2839189/1‑150 (MQ=255)

                                                                                                                          ggCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGa                      >  2:3866468/1‑150 (MQ=255)

                                                                                                                          ggCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGa                      >  1:618876/1‑150 (MQ=255)

                                                                                                                            cGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATc                    <  1:173589/150‑1 (MQ=255)

                                                                                                                            cGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATc                    >  2:2353886/1‑150 (MQ=255)

                                                                                                                             gTTGTCTGGTTTATTTTTAGACTGTAAATCAATTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCg                   <  1:1616695/150‑1 (MQ=255)

                                                                                                                              ttGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGa                  >  1:3051632/1‑150 (MQ=255)

                                                                                                                                gTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGata                <  1:1644016/150‑1 (MQ=255)

                                                                                                                                 tCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGatat               >  2:1080435/1‑150 (MQ=255)

                                                                                                                                  cTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATATAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATc              <  2:362571/150‑1 (MQ=255)

                                                                                                                                   tGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATcc             <  1:3288048/150‑1 (MQ=255)

                                                                                                                                    ggTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCt            <  2:2679383/150‑1 (MQ=255)

                                                                                                                                      tttATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGt          >  2:840242/1‑150 (MQ=255)

                                                                                                                                      tttATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGt          >  2:2713816/1‑150 (MQ=255)

                                                                                                                                        tATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGttt        <  2:1485035/150‑1 (MQ=255)

                                                                                                                                         aTTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTg       >  1:3455814/1‑150 (MQ=255)

                                                                                                                                           ttttAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAt     <  1:980753/150‑1 (MQ=255)

                                                                                                                                            tttAGACTGTAAATCACTTTTATATTGTATTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAtt    <  1:4374228/150‑1 (MQ=255)

                                                                                                                                              tAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTg  <  1:191846/150‑1 (MQ=255)

TTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTG  >  CP009273/320775‑321066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  >  CP009273/320775‑321068

TTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGT                                                                                                                                                  <  SRR19973082.236816/150‑1 (MQ=60)

 TTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTA                                                                                                                                                 <  SRR19973082.4117741/150‑1 (MQ=60)

  TCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAA                                                                                                                                                <  SRR19973082.1523403/150‑1 (MQ=60)

      TGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCA                                                                                                                                            <  SRR19973082.2769171/150‑1 (MQ=60)

      TGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCA                                                                                                                                            >  SRR19973082.3598524/1‑150 (MQ=60)

       GTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCAC                                                                                                                                           <  SRR19973082.650144/150‑1 (MQ=60)

       GTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCAC                                                                                                                                           <  SRR19973082.3034262/150‑1 (MQ=60)

          CTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTT                                                                                                                                        <  SRR19973082.664079/150‑1 (MQ=60)

          CTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTT                                                                                                                                        <  SRR19973082.644342/150‑1 (MQ=60)

                ATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATT                                                                                                                                  >  SRR19973082.1670065/1‑150 (MQ=60)

                  CTGGGCTGTTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGT                                                                                                                                <  SRR19973082.4412114/150‑1 (MQ=60)

                   TGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTC                                                                                                                               <  SRR19973082.1381193/150‑1 (MQ=60)

                                AAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAC                                                                                                                  <  SRR19973082.3236065/150‑1 (MQ=60)

                                AAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAC                                                                                                                  <  SRR19973082.3235351/150‑1 (MQ=60)

                                AAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAC                                                                                                                  <  SRR19973082.3235364/150‑1 (MQ=60)

                                  AAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTTGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAG                                                                                                                <  SRR19973082.3596493/150‑1 (MQ=60)

                                   AATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGA                                                                                                               >  SRR19973082.1576666/1‑150 (MQ=60)

                                   AATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGA                                                                                                               >  SRR19973082.2855116/1‑150 (MQ=60)

                                   AATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAAAGA                                                                                                               >  SRR19973082.2855112/1‑150 (MQ=60)

                                     TGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATACTGTAAATCACTTTTATATTTTCTTTATTTATATACAGAAA                                                                                                             >  SRR19973082.1332950/1‑150 (MQ=60)

                                     TGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAA                                                                                                             >  SRR19973082.2757778/1‑150 (MQ=60)

                                     TGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAA                                                                                                             >  SRR19973082.2378583/1‑150 (MQ=60)

                                     TGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAA                                                                                                             >  SRR19973082.375347/1‑150 (MQ=60)

                                                         CATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTAGTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATAC                                                                                         <  SRR19973082.1650506/150‑1 (MQ=60)

                                                            CTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGTTATACAGAAACAACAGCACCATAGCCATACATA                                                                                      >  SRR19973082.1788342/1‑150 (MQ=60)

                                                            CTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATA                                                                                      <  SRR19973082.2065595/150‑1 (MQ=60)

                                                               CAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTTTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAG                                                                                   >  SRR19973082.1460014/1‑150 (MQ=60)

                                                                AGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGC                                                                                  <  SRR19973082.2580883/150‑1 (MQ=60)

                                                                AGTCATTGTAGTCTGACAGGAAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGC                                                                                  <  SRR19973082.2580872/150‑1 (MQ=60)

                                                                  TCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCA                                                                                <  SRR19973082.1277143/150‑1 (MQ=60)

                                                                  TCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCA                                                                                <  SRR19973082.1277078/150‑1 (MQ=60)

                                                                    ATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATA                                                                              <  SRR19973082.1118884/150‑1 (MQ=60)

                                                                           TCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTTTGTTTTTTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAA                                                                       >  SRR19973082.1272783/1‑150 (MQ=60)

                                                                           TCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAA                                                                       >  SRR19973082.561463/1‑150 (MQ=60)

                                                                           TCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGAAAGTAAATCACTTTTATATTGTCTTTATTGTTATACAGAAACAACAGCACCATAGCCATACAAATATCCATAATAAAAA                                                                       >  SRR19973082.1272242/1‑150 (MQ=60)

                                                                               ACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGAT                                                                   <  SRR19973082.4456344/150‑1 (MQ=60)

                                                                                 AGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAA                                                                 >  SRR19973082.3954638/1‑150 (MQ=60)

                                                                                  GGAAAAATTATTATTGATATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAAC                                                                <  SRR19973082.2561526/150‑1 (MQ=60)

                                                                                    CAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACAC                                                              <  SRR19973082.1412333/150‑1 (MQ=60)

                                                                                    CAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACAC                                                              <  SRR19973082.3582935/150‑1 (MQ=60)

                                                                                        ATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAAT                                                          <  SRR19973082.3070099/150‑1 (MQ=60)

                                                                                          TATTATTGCTATCACCGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCT                                                        <  SRR19973082.4134619/150‑1 (MQ=60)

                                                                                              ATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTT                                                    >  SRR19973082.4331651/1‑150 (MQ=60)

                                                                                              ATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATATTTTT                                                    >  SRR19973082.4331646/1‑150 (MQ=60)

                                                                                               TTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTC                                                   <  SRR19973082.3110023/150‑1 (MQ=60)

                                                                                                       ACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATA                                           <  SRR19973082.813611/150‑1 (MQ=60)

                                                                                                        CAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAA                                          <  SRR19973082.1723135/150‑1 (MQ=60)

                                                                                                            TAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATA                                      <  SRR19973082.1372786/150‑1 (MQ=60)

                                                                                                            TAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATA                                      <  SRR19973082.1372588/150‑1 (MQ=60)

                                                                                                             AACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAA                                     <  SRR19973082.2056259/150‑1 (MQ=60)

                                                                                                                AAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAAT                                  <  SRR19973082.3811840/150‑1 (MQ=60)

                                                                                                                     TACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAAAGCCATACATATATCCATAATAAAAAGGATAACACGAATCTTTTTCTTAAGATAAAATAAAAACTAAA                             >  SRR19973082.272814/1‑150 (MQ=60)

                                                                                                                         TGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAG                         >  SRR19973082.2856621/1‑150 (MQ=60)

                                                                                                                          GGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGA                        >  SRR19973082.623237/1‑150 (MQ=60)

                                                                                                                          GGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATATCAATCAGA                        >  SRR19973082.3240453/1‑150 (MQ=60)

                                                                                                                            CGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATC                      <  SRR19973082.175077/150‑1 (MQ=60)

                                                                                                                             GTTGTCTGGTTTATTTTTAGACTGTAAATCAATTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCG                     <  SRR19973082.1627381/150‑1 (MQ=60)

                                                                                                                              TTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGA                    >  SRR19973082.3070357/1‑150 (MQ=60)

                                                                                                                                GTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATA                  <  SRR19973082.1654946/150‑1 (MQ=60)

                                                                                                                                   TGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCC               <  SRR19973082.3308346/150‑1 (MQ=60)

                                                                                                                                         ATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTG         >  SRR19973082.3477063/1‑150 (MQ=60)

                                                                                                                                         ATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTG         <  SRR19973082.1969895/150‑1 (MQ=60)

                                                                                                                                           TTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAT       <  SRR19973082.987580/150‑1 (MQ=60)

                                                                                                                                            TTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATT      <  SRR19973082.566881/150‑1 (MQ=60)

                                                                                                                                            TTTAGACTGTAAATCACTTTTATATTGTATTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATT      <  SRR19973082.4400550/150‑1 (MQ=60)

                                                                                                                                              TAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTG    <  SRR19973082.193466/150‑1 (MQ=60)

                                                                                                                                                GACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  <  SRR19973082.1068438/150‑1 (MQ=60)

                                                                                                                                                GACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  <  SRR19973082.1068351/150‑1 (MQ=60)

                                                                                                                                                GACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  <  SRR19973082.1068342/150‑1 (MQ=60)

                                                                                                                                                GACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  <  SRR19973082.1068328/150‑1 (MQ=60)

TTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  >  CP009273/320775‑321068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`