Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 320,920 G→A 100% S52S (AGC→AGT)  ykgH ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273320,9200GA97.1% 483.2 / 3.2 141S52S (AGC→AGT) ykgHputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (60/76);  total (62/79)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 141 total aligned reads displayed.

TATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTG  >  CP009273/320773‑321066
                                                                                                                                                   |                                                                                                                                                  
tATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACt                                                                                                                                                  <  2:3457279/150‑1 (MQ=255)
 aTTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTg                                                                                                                                                 <  2:1447650/150‑1 (MQ=255)
 aTTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTg                                                                                                                                                 <  2:2374015/150‑1 (MQ=255)
 aTTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTg                                                                                                                                                 <  2:4777092/150‑1 (MQ=255)
 aTTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTg                                                                                                                                                 <  2:675578/150‑1 (MQ=255)
   ttCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTa                                                                                                                                               >  1:1146490/1‑150 (MQ=255)
    tctcTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTaa                                                                                                                                              <  1:364349/150‑1 (MQ=255)
       ctGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATc                                                                                                                                           <  2:2518736/150‑1 (MQ=255)
         gTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCAc                                                                                                                                         <  2:1352991/150‑1 (MQ=255)
         gTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCAc                                                                                                                                         >  1:1462326/1‑150 (MQ=255)
          ttCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACt                                                                                                                                        <  2:949622/150‑1 (MQ=255)
             ttGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACtttt                                                                                                                                     <  1:758675/150‑1 (MQ=255)
             ttGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACtttt                                                                                                                                     >  2:4538579/1‑150 (MQ=255)
              tGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTta                                                                                                                                    <  2:2268699/150‑1 (MQ=255)
               gACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTtat                                                                                                                                   >  1:1707767/1‑150 (MQ=255)
                      gggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCt                                                                                                                            <  1:1604909/150‑1 (MQ=255)
                      gggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCt                                                                                                                            <  1:2281668/150‑1 (MQ=255)
                      gggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCt                                                                                                                            <  1:1606446/150‑1 (MQ=255)
                       ggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCtt                                                                                                                           <  2:1084922/150‑1 (MQ=255)
                        gCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCttt                                                                                                                          <  2:2898143/150‑1 (MQ=255)
                        gCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCttt                                                                                                                          <  2:506111/150‑1 (MQ=255)
                         cTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTa                                                                                                                         <  2:3597297/150‑1 (MQ=255)
                         cTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTa                                                                                                                         <  2:3597309/150‑1 (MQ=255)
                          tGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTAt                                                                                                                        <  2:876214/150‑1 (MQ=255)
                          tGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATAGTCTTTAt                                                                                                                        >  1:3240730/1‑150 (MQ=255)
                               tgAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGata                                                                                                                   <  1:1904384/150‑1 (MQ=255)
                               tgAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGata                                                                                                                   >  1:3497330/1‑150 (MQ=255)
                                  aaaaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAc                                                                                                                <  1:2473161/150‑1 (MQ=255)
                                  aaaaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAc                                                                                                                <  1:12036/150‑1 (MQ=255)
                                  aaaaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAc                                                                                                                <  1:857264/150‑1 (MQ=255)
                                     aaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGa                                                                                                             <  1:2345622/150‑1 (MQ=255)
                                     aaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGa                                                                                                             <  2:269543/150‑1 (MQ=255)
                                       tGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGaaa                                                                                                           <  1:3302768/150‑1 (MQ=255)
                                        gtagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaac                                                                                                          <  2:605474/150‑1 (MQ=255)
                                         tagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaaca                                                                                                         <  1:4478546/150‑1 (MQ=255)
                                         tagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaaca                                                                                                         <  1:1089422/150‑1 (MQ=255)
                                         tagtaAGTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaaca                                                                                                         <  1:4478598/150‑1 (MQ=255)
                                           gtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaacaac                                                                                                       <  1:2305852/150‑1 (MQ=255)
                                             aaTTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAg                                                                                                     >  2:2327595/1‑150 (MQ=255)
                                              aTTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGc                                                                                                    >  2:2604267/1‑150 (MQ=255)
                                              aTTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGc                                                                                                    <  1:3173056/150‑1 (MQ=255)
                                               ttCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCa                                                                                                   <  1:644750/150‑1 (MQ=255)
                                                   cTGAATAACATCTTCAGTCATTTTAGTCTGACAGTCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAt                                                                                               <  2:4347677/150‑1 (MQ=255)
                                                   cTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAt                                                                                               <  2:1946763/150‑1 (MQ=255)
                                                   cTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAt                                                                                               >  1:800580/1‑150 (MQ=255)
                                                       aTAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGcc                                                                                           >  2:1096143/1‑150 (MQ=255)
                                                        tAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCca                                                                                          <  1:2771248/150‑1 (MQ=255)
                                                        tAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCca                                                                                          <  1:2771221/150‑1 (MQ=255)
                                                        tAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCca                                                                                          >  1:350447/1‑150 (MQ=255)
                                                          aCATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGCCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcata                                                                                        <  2:1713934/150‑1 (MQ=255)
                                                            aTCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcataca                                                                                      >  2:4544673/1‑150 (MQ=255)
                                                             tCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcatacat                                                                                     <  1:1748013/150‑1 (MQ=255)
                                                               ttCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTTGTTTATTTTTAGACTGTAAAACACTTTTATAATGTCTTTATAGATATACAGACAAAACAGCACCATAGCaatacaaaa                                                                                   >  1:3407018/1‑147 (MQ=255)
                                                               ttCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTTTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACatat                                                                                   >  1:3833219/1‑150 (MQ=255)
                                                               ttCAGTCATTGTAGTCTGACAGGCAAAAATATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCAGGTTTATTTTTAGACTGTAAAAAACATATATATTTTCATTATTTATATAAAGAAACAACAGAACCAAAGCcaaaaaaaa                                                                                   >  1:3406197/1‑143 (MQ=255)
                                                                     cATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCat                                                                             >  2:2743112/1‑150 (MQ=255)
                                                                     cATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCat                                                                             <  1:2764665/150‑1 (MQ=255)
                                                                      aTTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTTTTATGTAAATAACATTTATATTGTCTTTATTGATATAAATAAAAAACAGCACCAAAACCAAAAAAATAGcccaa                                                                            >  1:3595154/1‑147 (MQ=255)
                                                                       ttGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataa                                                                           <  2:1679307/150‑1 (MQ=255)
                                                                       ttGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataa                                                                           <  2:2981018/150‑1 (MQ=255)
                                                                        tGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataat                                                                          <  1:3314455/150‑1 (MQ=255)
                                                                        tGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataat                                                                          <  1:4065106/150‑1 (MQ=255)
                                                                        tGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataat                                                                          >  1:1841691/1‑150 (MQ=255)
                                                                        tGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataat                                                                          <  2:2176540/150‑1 (MQ=255)
                                                                         gTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTTATATACAGAAACAACAGCACCATAGCCATACATATATCCataata                                                                         >  1:2060468/1‑150 (MQ=255)
                                                                         gTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataata                                                                         >  1:2060467/1‑150 (MQ=255)
                                                                            gTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCCTACATATAGCCATAATaaaa                                                                      >  1:2245189/1‑150 (MQ=255)
                                                                               tGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAgg                                                                   >  1:1075777/1‑150 (MQ=255)
                                                                                 aCAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGAt                                                                 <  2:3823731/150‑1 (MQ=255)
                                                                                       aaaaTTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACg                                                           >  2:2408898/1‑150 (MQ=255)
                                                                                         aaTTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGaa                                                         <  1:1782334/150‑1 (MQ=255)
                                                                                          attattattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAAt                                                        <  2:4530071/150‑1 (MQ=255)
                                                                                             attattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCtt                                                     <  2:111673/150‑1 (MQ=255)
                                                                                              ttattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttt                                                    >  1:1500516/1‑150 (MQ=255)
                                                                                              ttattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttt                                                    <  2:390558/150‑1 (MQ=255)
                                                                                               tattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCtttt                                                   >  2:489757/1‑150 (MQ=255)
                                                                                                attGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttttt                                                  >  2:1346064/1‑150 (MQ=255)
                                                                                                attGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttttt                                                  >  2:1988355/1‑150 (MQ=255)
                                                                                                  tGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTttct                                                >  2:1186732/1‑150 (MQ=255)
                                                                                                     tATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCa                                             >  2:311261/1‑150 (MQ=255)
                                                                                                           aGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACATCACCATAGCCATACAAATATCCATAATAAAAAGGATAACACGAATATTTTTATTCAGataaa                                       >  1:4646401/1‑150 (MQ=255)
                                                                                                           aGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaa                                       <  2:2902066/150‑1 (MQ=255)
                                                                                                              tAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaata                                    >  2:3123601/1‑150 (MQ=255)
                                                                                                                 cAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAATGATAACACGAATCTTTTTCTTAAGAtaaaataaaa                                 >  2:101240/1‑150 (MQ=255)
                                                                                                                      ataCCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCa                            <  2:3240730/150‑1 (MQ=255)
                                                                                                                        aCCTGGCGTTGTCTGGTTTATTTTTATACAGTAAATAAATTTTATATTTTCTTTATTTATATAAAGAAACAAAAGCACCATAGCCATACATATATCCATAATAAAAAGGATAAAACGAATCTTTTTATTAAGATAAAATAAAATATCAAt                          >  1:2977816/1‑150 (MQ=255)
                                                                                                                               gTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCg                   >  1:3565328/1‑150 (MQ=255)
                                                                                                                               gTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCg                   >  1:3565337/1‑150 (MQ=255)
                                                                                                                                 tGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGat                 <  1:2257253/150‑1 (MQ=255)
                                                                                                                                  gTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGata                >  1:561569/1‑150 (MQ=255)
                                                                                                                                  gTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGata                >  1:3487006/1‑150 (MQ=255)
                                                                                                                                  gTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGata                <  1:4011259/150‑1 (MQ=255)
                                                                                                                                    cTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATc              >  2:4118676/1‑150 (MQ=255)
                                                                                                                                    cTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATc              >  2:4118704/1‑150 (MQ=255)
                                                                                                                                      ggTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCt            >  2:264352/1‑150 (MQ=255)
                                                                                                                                       gTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTg           >  1:704692/1‑150 (MQ=255)
                                                                                                                                         ttATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATaaaataaaat                                >  2:4527851/1‑127 (MQ=255)
                                                                                                                                         ttATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGtt         >  1:2705273/1‑150 (MQ=255)
                                                                                                                                           aTTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTg       >  1:4575113/1‑150 (MQ=255)
                                                                                                                                           aTTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTg       >  2:3953147/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTG  >  CP009273/320773‑321066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTTC  >  CP009273/320771‑321069
                                                                                                                                                     |                                                                                                                                                     
CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGA                                                                                                                                                       >  SRR19973078.1197301/1‑150 (MQ=60)
     TTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTA                                                                                                                                                  >  SRR19973078.1153353/1‑150 (MQ=60)
      TCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAA                                                                                                                                                 <  SRR19973078.366610/150‑1 (MQ=60)
         CTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATC                                                                                                                                              >  SRR19973078.1706752/1‑150 (MQ=60)
           GTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCAC                                                                                                                                            >  SRR19973078.1471292/1‑150 (MQ=60)
               TTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTT                                                                                                                                        <  SRR19973078.763190/150‑1 (MQ=60)
               TTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTT                                                                                                                                        <  SRR19973078.2249577/150‑1 (MQ=60)
                 GACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTAT                                                                                                                                      >  SRR19973078.1718163/1‑150 (MQ=60)
                  ACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATA                                                                                                                                     <  SRR19973078.3486278/150‑1 (MQ=60)
                  ACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATA                                                                                                                                     <  SRR19973078.1171147/150‑1 (MQ=60)
                     TCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTG                                                                                                                                  <  SRR19973078.400679/150‑1 (MQ=60)
                        GGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCT                                                                                                                               <  SRR19973078.1614477/150‑1 (MQ=60)
                        GGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCT                                                                                                                               <  SRR19973078.1616030/150‑1 (MQ=60)
                        GGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCT                                                                                                                               <  SRR19973078.2294809/150‑1 (MQ=60)
                        GGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCT                                                                                                                               <  SRR19973078.2295217/150‑1 (MQ=60)
                            TGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACATTTATATATTCTTTAT                                                                                                                           >  SRR19973078.3258987/1‑150 (MQ=60)
                               TGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGA                                                                                                                        >  SRR19973078.4723807/1‑150 (MQ=60)
                                 TGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATA                                                                                                                      <  SRR19973078.1915653/150‑1 (MQ=60)
                                 TGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATA                                                                                                                      >  SRR19973078.3517206/1‑150 (MQ=60)
                                    AAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAC                                                                                                                   <  SRR19973078.862480/150‑1 (MQ=60)
                                    AAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAC                                                                                                                   <  SRR19973078.2487350/150‑1 (MQ=60)
                                    AAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAC                                                                                                                   <  SRR19973078.12194/150‑1 (MQ=60)
                                       AATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGA                                                                                                                <  SRR19973078.2358937/150‑1 (MQ=60)
                                         TGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAA                                                                                                              <  SRR19973078.3321606/150‑1 (MQ=60)
                                           tatttATTCGCTGAATAAAATCTTCAGTCATTGTAGTCTGACATGCAAAATTATTATTGCTATCACAGCTAAATATTATACCTTTCGTTGTCTTGTTTATTTTTAGACTGTAAATCAATTGTATATTGTCTTTATTGATATACAGtaatt                                                                                                            <  SRR19973078.1001178/145‑6 (MQ=60)
                                           TAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                            <  SRR19973078.4503894/150‑1 (MQ=60)
                                           TAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                            <  SRR19973078.1096081/150‑1 (MQ=60)
                                           TAGTAAGTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                            <  SRR19973078.4503946/150‑1 (MQ=60)
                                             GTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAAC                                                                                                          <  SRR19973078.2319069/150‑1 (MQ=60)
                                               AATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAG                                                                                                        >  SRR19973078.2454481/1‑150 (MQ=60)
                                                ATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGC                                                                                                       <  SRR19973078.3192051/150‑1 (MQ=60)
                                                ATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGC                                                                                                       <  SRR19973078.3190727/150‑1 (MQ=60)
                                                 TTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCA                                                                                                      <  SRR19973078.648872/150‑1 (MQ=60)
                                                     CTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAT                                                                                                  >  SRR19973078.805219/1‑150 (MQ=60)
                                                          TAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCA                                                                                             <  SRR19973078.2786696/150‑1 (MQ=60)
                                                          TAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCA                                                                                             <  SRR19973078.2786724/150‑1 (MQ=60)
                                                          TAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCA                                                                                             >  SRR19973078.352786/1‑150 (MQ=60)
                                                          TAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCA                                                                                             >  SRR19973078.352663/1‑150 (MQ=60)
                                                               TCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAT                                                                                        <  SRR19973078.1758660/150‑1 (MQ=60)
                                                                 TTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTTGTTTATTTTTAGACTGTAAAACACTTTTATAATGTCTTTATAGATATACAGACAAAACAGCACCATAGCAATACAaaa                                                                                      >  SRR19973078.3426509/1‑147 (MQ=60)
                                                                 TTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTTTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATAT                                                                                      >  SRR19973078.3855178/1‑150 (MQ=60)
                                                                 TTCAGTCATTGTAGTCTGACAGGCAAAAATATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCAGGTTTATTTTTAGACTGTAAAAAACATATATATTTTCATTATTTATATAAAGAAACAACAGAACCAAAGCCAaaaaaaa                                                                                      >  SRR19973078.3425684/1‑143 (MQ=60)
                                                                       CATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCAT                                                                                <  SRR19973078.2780118/150‑1 (MQ=60)
                                                                        ATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTTTTATGTAAATAACATTTATATTGTCTTTATTGATATAAATAAAAAACAGCACCAaaaccaaaaaaatagcccaa                                                                               >  SRR19973078.3615326/1‑130 (MQ=60)
                                                                          TGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAAT                                                                             >  SRR19973078.1852742/1‑150 (MQ=60)
                                                                          TGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAAT                                                                             <  SRR19973078.4088262/150‑1 (MQ=60)
                                                                          TGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAAT                                                                             <  SRR19973078.3333380/150‑1 (MQ=60)
                                                                           GTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTTATATACAGAAACAACAGCACCATAGCCATACATATATCCATAATA                                                                            >  SRR19973078.2072585/1‑150 (MQ=60)
                                                                           GTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATA                                                                            >  SRR19973078.2072584/1‑150 (MQ=60)
                                                                              GTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCCTACATATAGCCATAATAAAA                                                                         >  SRR19973078.2258214/1‑150 (MQ=60)
                                                                              GTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTGGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAA                                                                         >  SRR19973078.1777107/1‑150 (MQ=60)
                                                                                 TGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGG                                                                      >  SRR19973078.1082386/1‑150 (MQ=60)
                                                                                           AATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAA                                                            <  SRR19973078.1793141/150‑1 (MQ=60)
                                                                                                TTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGTATAACACGAATCTTT                                                       >  SRR19973078.1509620/1‑150 (MQ=60)
                                                                                                TTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTT                                                       <  SRR19973078.2961311/150‑1 (MQ=60)
                                                                                                    TGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCT                                                   <  SRR19973078.3411740/150‑1 (MQ=60)
                                                                                                      CTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTC                                                 <  SRR19973078.1493205/150‑1 (MQ=60)
                                                                                                      CTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTC                                                 <  SRR19973078.734295/150‑1 (MQ=60)
                                                                                                           ACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATA                                            <  SRR19973078.2006358/150‑1 (MQ=60)
                                                                                                             AGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACATCACCATAGCCATACAAATATCCATAATAAAAAGGATAACACGAATATTTTTATTCAGATAAA                                          >  SRR19973078.4672697/1‑150 (MQ=60)
                                                                                                                  ACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAA                                     <  SRR19973078.3590901/150‑1 (MQ=60)
                                                                                                                    tcagacgtgtgctcttccgatcTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAAT                                   <  SRR19973078.4553495/128‑1 (MQ=60)
                                                                                                                          ACCTGGCGTTGTCTGGTTTATTTTTATACAGTAAATAAATTTTATATTTTCTTTATTTATATAAAGAAACAAAAGCACCATAGCCATACATATATCCATAATAAAAAGGATAAAACGAATCTTTTTATTAAGATAAAATAAAATATCAAT                             >  SRR19973078.2994806/1‑150 (MQ=60)
                                                                                                                                 GTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCG                      >  SRR19973078.3585413/1‑150 (MQ=60)
                                                                                                                                 GTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCG                      >  SRR19973078.3585404/1‑150 (MQ=60)
                                                                                                                                   TGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGAT                    <  SRR19973078.2270321/150‑1 (MQ=60)
                                                                                                                                    GTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATA                   >  SRR19973078.3506848/1‑150 (MQ=60)
                                                                                                                                    GTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATA                   >  SRR19973078.565287/1‑150 (MQ=60)
                                                                                                                                    GTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATA                   >  SRR19973078.4112271/1‑150 (MQ=60)
                                                                                                                                    GTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATA                   <  SRR19973078.4033940/150‑1 (MQ=60)
                                                                                                                                         GTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTG              >  SRR19973078.709038/1‑150 (MQ=60)
                                                                                                                                           TTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTT            >  SRR19973078.2720569/1‑150 (MQ=60)
                                                                                                                                           TTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTT            >  SRR19973078.2720583/1‑150 (MQ=60)
                                                                                                                                             ATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTG          >  SRR19973078.4601049/1‑150 (MQ=60)
                                                                                                                                                 TTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCTATATACTGTTTGATTT      >  SRR19973078.633089/1‑150 (MQ=60)
                                                                                                                                                   AGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGT    <  SRR19973078.4460799/150‑1 (MQ=60)
                                                                                                                                                     ACTGTAAATCACTTTTATTTTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCTATCAGATCGATATCCTGTTTGATTTGTTC  <  SRR19973078.4280557/150‑1 (MQ=60)
                                                                                                                                                     ACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTTC  <  SRR19973078.4282202/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTTC  >  CP009273/320771‑321069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑