Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F121 I0 R1	856	273.6	9081716	94.1%	8545894	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	320,920	G→A	89.3%	S52S (AGC→AGT)	ykgH ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	320,920	0	G	A	89.3%	479.3 / 39.3	158	S52S (AGC→AGT)	ykgH	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (8/9); new base A (59/82); total (67/91)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

TATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTT  >  CP009273/320773‑321065

tATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACt                                                                                                                                                 <  1:2057984/150‑1 (MQ=255)

 aTTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTg                                                                                                                                                <  1:2696623/150‑1 (MQ=255)

   ttCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTa                                                                                                                                              <  2:1384393/150‑1 (MQ=255)

   ttCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTa                                                                                                                                              <  1:349741/150‑1 (MQ=255)

      tctGTTCTTGACATCTGGTCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAAt                                                                                                                                           <  2:3093376/150‑1 (MQ=255)

        tGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCa                                                                                                                                         <  2:443415/150‑1 (MQ=255)

        tGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCa                                                                                                                                         >  1:3294668/1‑150 (MQ=255)

          ttCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACt                                                                                                                                       <  1:2935365/150‑1 (MQ=255)

            cTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACttt                                                                                                                                     >  2:987752/1‑150 (MQ=255)

            cTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACttt                                                                                                                                     >  2:2265635/1‑150 (MQ=255)

              tGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTta                                                                                                                                   <  2:1056170/150‑1 (MQ=255)

                 cATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTatat                                                                                                                                >  1:2903966/1‑150 (MQ=255)

                     tGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATAATTTAAATAACATTTATAtttta                                                                                                                            >  1:3475695/1‑147 (MQ=255)

                     tGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTc                                                                                                                            <  2:1805631/150‑1 (MQ=255)

                      gggCTGGTGTGAAAATATGTAGTAATTCTCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCTAAATTATTATTGCTATCACAGCTAACATGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCt                                                                                                                           <  1:666090/150‑1 (MQ=255)

                      gggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAATTCACTTTTATATTGTCt                                                                                                                           >  2:255150/1‑150 (MQ=255)

                       ggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCtt                                                                                                                          <  1:4130408/150‑1 (MQ=255)

                        gCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCttt                                                                                                                         <  2:2590203/150‑1 (MQ=255)

                         cTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTa                                                                                                                        <  2:2368959/150‑1 (MQ=255)

                          tGGTGTGAAAAATTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTAt                                                                                                                       <  1:1475175/150‑1 (MQ=255)

                          tGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCGGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTAt                                                                                                                       >  2:3128742/1‑150 (MQ=255)

                          tGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTg                                                                                                                              <  2:2055508/143‑1 (MQ=255)

                          tGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTAt                                                                                                                       <  2:4011584/150‑1 (MQ=255)

                              gtgAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGat                                                                                                                   >  2:4363844/1‑150 (MQ=255)

                              gtgAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGat                                                                                                                   >  2:4363422/1‑150 (MQ=255)

                                  aaaaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAc                                                                                                               <  1:3730700/150‑1 (MQ=255)

                                   aaaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACa                                                                                                              <  2:2125273/150‑1 (MQ=255)

                                    aaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAg                                                                                                             >  2:3851746/1‑150 (MQ=255)

                                    aaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTGTCTTTATTGATATACAg                                                                                                             >  2:3851447/1‑150 (MQ=255)

                                      aTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGaa                                                                                                           >  2:668911/1‑150 (MQ=255)

                                        gtagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTGGTGTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAAAGAaac                                                                                                         >  1:4258251/1‑150 (MQ=255)

                                         tagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaaca                                                                                                        >  1:544315/1‑150 (MQ=255)

                                         tagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaaca                                                                                                        <  1:379824/150‑1 (MQ=255)

                                         tagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaaca                                                                                                        <  1:379767/150‑1 (MQ=255)

                                                    tGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATa                                                                                             <  1:268905/150‑1 (MQ=255)

                                                     gAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAg                                                                                            <  1:3822686/150‑1 (MQ=255)

                                                       aTAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGcc                                                                                          >  1:3781096/1‑150 (MQ=255)

                                                       aTAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGcc                                                                                          <  2:2066778/150‑1 (MQ=255)

                                                       aTAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTTTCTTTATTGATATACAGAAACAACAGCACCATAGcc                                                                                          >  1:177626/1‑150 (MQ=255)

                                                        tAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCca                                                                                         <  2:3221805/150‑1 (MQ=255)

                                                         aaCATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcat                                                                                        <  2:4019662/150‑1 (MQ=255)

                                                         aaCATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTGTCTTTATTTATATACAGAAAAAACAGCACCATAGCcat                                                                                        >  1:3184778/1‑150 (MQ=255)

                                                           cATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcatac                                                                                      <  2:4533666/150‑1 (MQ=255)

                                                           cATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcatac                                                                                      >  2:2420988/1‑150 (MQ=255)

                                                            aTCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcataca                                                                                     >  2:1159929/1‑150 (MQ=255)

                                                              cTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcatacata                                                                                   >  2:1377934/1‑150 (MQ=255)

                                                                tCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAtata                                                                                 <  1:2074442/150‑1 (MQ=255)

                                                                tCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAtata                                                                                 <  1:4351265/150‑1 (MQ=255)

                                                                tCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAtata                                                                                 <  1:1208941/150‑1 (MQ=255)

                                                                  aGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGc                                                                               <  1:448406/150‑1 (MQ=255)

                                                                  aGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGc                                                                               <  1:2416027/150‑1 (MQ=255)

                                                                   gTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGcc                                                                              <  2:1111418/150‑1 (MQ=255)

                                                                      aTTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCata                                                                           >  2:865746/1‑150 (MQ=255)

                                                                        tGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataat                                                                         <  1:321102/150‑1 (MQ=255)

                                                                              cTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAg                                                                   <  2:3710506/150‑1 (MQ=255)

                                                                               tGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAgg                                                                  >  2:4137113/1‑150 (MQ=255)

                                                                                gACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGa                                                                 >  2:709750/1‑150 (MQ=255)

                                                                                 aCAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATATCCATACATATAGCCATAATAAAAAGGAt                                                                >  1:2779743/1‑150 (MQ=255)

                                                                                 aCAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGAt                                                                <  1:484953/150‑1 (MQ=255)

                                                                                  cAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATa                                                               >  2:3000210/1‑150 (MQ=255)

                                                                                   aGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATaa                                                              <  2:3436243/150‑1 (MQ=255)

                                                                                   aGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATaa                                                              >  2:694549/1‑150 (MQ=255)

                                                                                     gCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAaca                                                            <  1:3319893/150‑1 (MQ=255)

                                                                                     gCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAaca                                                            <  2:1274702/150‑1 (MQ=255)

                                                                                      cAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAacac                                                           <  1:2567050/150‑1 (MQ=255)

                                                                                      cAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAacac                                                           <  1:1358066/150‑1 (MQ=255)

                                                                                          attattattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAAAACGAAt                                                       >  2:1170370/1‑150 (MQ=255)

                                                                                           ttattattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATc                                                      <  2:3728005/150‑1 (MQ=255)

                                                                                            tattattTCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTTTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCt                                                     <  1:3580224/150‑1 (MQ=255)

                                                                                            tattattGCTATCACAGATAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCt                                                     <  1:3578269/150‑1 (MQ=255)

                                                                                              ttattTCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttt                                                   <  2:3146630/150‑1 (MQ=255)

                                                                                                attGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGTTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttttt                                                 <  1:383526/150‑1 (MQ=255)

                                                                                                 ttGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTttc                                                <  1:366309/150‑1 (MQ=255)

                                                                                                      aTCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAg                                           >  2:120165/1‑150 (MQ=255)

                                                                                                       tCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAATGATAACACGAATCTTTTTCTTCAGa                                          >  1:4084824/1‑150 (MQ=255)

                                                                                                          caGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataa                                       <  1:1236171/150‑1 (MQ=255)

                                                                                                          caGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAAAGCCATACATATAGCCCTAATAAAAAGGATAACACGAAACTTTTTCTTCAGataa                                       >  1:2078156/1‑150 (MQ=255)

                                                                                                           aGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaa                                      >  2:3019761/1‑150 (MQ=255)

                                                                                                            gCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaa                                     >  2:2221527/1‑150 (MQ=255)

                                                                                                               aaCAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaataa                                  >  2:3778791/1‑150 (MQ=255)

                                                                                                                   aGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAAtc                              >  2:1190781/1‑150 (MQ=255)

                                                                                                                    gTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAAtct                             <  1:3675031/150‑1 (MQ=255)

                                                                                                                      ataCCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCa                           <  2:2369593/150‑1 (MQ=255)

                                                                                                                        aCCTGGCGTTGTCTTGTTTATTTTTAGGCTGTAAAAAACATTTATATTTTAATTATTTATAAAAAGAAACAAAAGCACCAAATCCAAACAAAAAGCCAAAAAAAAAAAGAAAACACGAATAAATTTATTAATAtaaaaaaaaaaataaaa                         >  1:204706/1‑143 (MQ=255)

                                                                                                                         ccTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACATTTATATTGTATTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATc                        >  1:838588/1‑150 (MQ=255)

                                                                                                                          cTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCa                       <  2:3294668/150‑1 (MQ=255)

                                                                                                                           tGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTTTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAg                      >  1:1961575/1‑150 (MQ=255)

                                                                                                                           tGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAg                      <  1:2563154/150‑1 (MQ=255)

                                                                                                                               gTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCg                  <  2:899315/150‑1 (MQ=255)

                                                                                                                                     tgttttATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATcc            <  1:1025588/147‑1 (MQ=255)

                                                                                                                                     tgttttATTTTTAGACTGTAAATAACTTTTATATTGTATTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATcc            <  1:2072768/147‑1 (MQ=255)

                                                                                                                                       gTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTg          >  1:1029763/1‑150 (MQ=255)

                                                                                                                                       gTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTg          >  1:487238/1‑150 (MQ=255)

                                                                                                                                           aTTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTg      >  1:3348791/1‑150 (MQ=255)

                                                                                                                                             ttttAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAt    <  2:559466/150‑1 (MQ=255)

                                                                                                                                             ttttAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAt    <  2:559521/150‑1 (MQ=255)

                                                                                                                                             ttttAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAt    >  1:4165287/1‑150 (MQ=255)

                                                                                                                                               ttAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAttt  <  2:3582060/150‑1 (MQ=255)

                                                                                                                                               ttAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCCGATCGATATCCTGTTTGAttt  <  2:155098/150‑1 (MQ=255)

                                                                                                                                               ttAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAttt  <  1:3851746/150‑1 (MQ=255)

TATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTT  >  CP009273/320773‑321065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  >  CP009273/320771‑321068

CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGA                                                                                                                                                      <  SRR19973080.1640522/150‑1 (MQ=60)

CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGA                                                                                                                                                      <  SRR19973080.1639002/150‑1 (MQ=60)

  TATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACT                                                                                                                                                    <  SRR19973080.2069665/150‑1 (MQ=60)

   ATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTG                                                                                                                                                   <  SRR19973080.188329/150‑1 (MQ=60)

   ATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTG                                                                                                                                                   <  SRR19973080.2711377/150‑1 (MQ=60)

     TTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTA                                                                                                                                                 >  SRR19973080.127588/1‑150 (MQ=60)

     TTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTA                                                                                                                                                 <  SRR19973080.351793/150‑1 (MQ=60)

          TGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCA                                                                                                                                            >  SRR19973080.3312722/1‑150 (MQ=60)

            TTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACT                                                                                                                                          <  SRR19973080.2951323/150‑1 (MQ=60)

             TCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTT                                                                                                                                         <  SRR19973080.2126385/150‑1 (MQ=60)

                TGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTA                                                                                                                                      >  SRR19973080.2443599/1‑150 (MQ=60)

                   CATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATAT                                                                                                                                   >  SRR19973080.2919388/1‑150 (MQ=60)

                   CATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATAT                                                                                                                                   >  SRR19973080.2919814/1‑150 (MQ=60)

                       TGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATACTGTAAATCACATTTATATTGTC                                                                                                                               >  SRR19973080.1196921/1‑150 (MQ=60)

                        GGGCTGGTGTGAAAATATGTAGTAATTCTCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCTAAATTATTATTGCTATCACAGCTAACATGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCT                                                                                                                              <  SRR19973080.670063/150‑1 (MQ=60)

                         GGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTT                                                                                                                             <  SRR19973080.4152782/150‑1 (MQ=60)

                            TGGTGTGAAAAATTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTAT                                                                                                                          <  SRR19973080.1483645/150‑1 (MQ=60)

                            TGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATACTGTAAATCACTTTTATATTtagatcgg                                                                                                                          >  SRR19973080.2067176/1‑142 (MQ=60)

                                    AAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAC                                                                                                                  <  SRR19973080.3751121/150‑1 (MQ=60)

                                      AAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAG                                                                                                                >  SRR19973080.497582/1‑150 (MQ=60)

                                       AATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGA                                                                                                               >  SRR19973080.4409217/1‑150 (MQ=60)

                                          GTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTGGTGTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAAAGAAAC                                                                                                            >  SRR19973080.4281477/1‑150 (MQ=60)

                                           TAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                           >  SRR19973080.258728/1‑150 (MQ=60)

                                           TAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                           <  SRR19973080.381966/150‑1 (MQ=60)

                                           TAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                           <  SRR19973080.381909/150‑1 (MQ=60)

                                           TAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                           >  SRR19973080.547779/1‑150 (MQ=60)

                                           TAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                           <  SRR19973080.381913/150‑1 (MQ=60)

                                                      TGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATA                                                                                                <  SRR19973080.270694/150‑1 (MQ=60)

                                                       GAATAACATATTCTGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAG                                                                                               <  SRR19973080.3844686/150‑1 (MQ=60)

                                                       GAATAAAATCTTCAGTAATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAG                                                                                               <  SRR19973080.3843629/150‑1 (MQ=60)

                                                         ATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCC                                                                                             >  SRR19973080.3801635/1‑150 (MQ=60)

                                                         ATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCC                                                                                             >  SRR19973080.3801636/1‑150 (MQ=60)

                                                         ATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTTTCTTTATTGATATACAGAAACAACAGCACCATAGCC                                                                                             >  SRR19973080.179020/1‑150 (MQ=60)

                                                           AACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTGTCTTTATTTATATACAGAAAAAACAGCACCATAGCCAT                                                                                           >  SRR19973080.3202360/1‑150 (MQ=60)

                                                               TCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAT                                                                                       <  SRR19973080.353950/150‑1 (MQ=60)

                                                                  TCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATA                                                                                    <  SRR19973080.2086196/150‑1 (MQ=60)

                                                                  TCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATA                                                                                    <  SRR19973080.1216248/150‑1 (MQ=60)

                                                                  TCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATA                                                                                    <  SRR19973080.4375017/150‑1 (MQ=60)

                                                                  TCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATA                                                                                    <  SRR19973080.2086101/150‑1 (MQ=60)

                                                                    AGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGC                                                                                  <  SRR19973080.2429438/150‑1 (MQ=60)

                                                                    AGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGC                                                                                  <  SRR19973080.451222/150‑1 (MQ=60)

                                                                    AGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGC                                                                                  <  SRR19973080.451425/150‑1 (MQ=60)

                                                                        ATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATA                                                                              <  SRR19973080.2773587/150‑1 (MQ=60)

                                                                          TGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAAT                                                                            <  SRR19973080.323080/150‑1 (MQ=60)

                                                                           GTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATA                                                                           <  SRR19973080.3275646/150‑1 (MQ=60)

                                                                                   ACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATATCCATACATATAGCCATAATAAAAAGGAT                                                                   >  SRR19973080.2795082/1‑150 (MQ=60)

                                                                                   ACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGAT                                                                   <  SRR19973080.488039/150‑1 (MQ=60)

                                                                                       GCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACA                                                               <  SRR19973080.3338018/150‑1 (MQ=60)

                                                                                       GCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACA                                                               <  SRR19973080.3338063/150‑1 (MQ=60)

                                                                                        CAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACAC                                                              <  SRR19973080.2581073/150‑1 (MQ=60)

                                                                                        CAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACAC                                                              <  SRR19973080.3663382/150‑1 (MQ=60)

                                                                                        CAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACAC                                                              <  SRR19973080.1366199/150‑1 (MQ=60)

                                                                                        CAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACAC                                                              <  SRR19973080.4147479/150‑1 (MQ=60)

                                                                                              TATTATTTCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTTTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCT                                                        <  SRR19973080.3600023/150‑1 (MQ=60)

                                                                                              TATTATTGCTATCACAGATAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCT                                                        <  SRR19973080.3598059/150‑1 (MQ=60)

                                                                                                  ATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGTTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTT                                                    <  SRR19973080.385697/150‑1 (MQ=60)

                                                                                                  ATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTTTTTTTAGACTGTAAATAACTTTTATATTGTCTTTATTGATATAAAGAAACAACAGCACCAAAGCCAAACAAATAGCCAAAATAAAAATGATAAAACGAATATTTTT                                                    >  SRR19973080.1537773/1‑150 (MQ=60)

                                                                                                   TTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTC                                                   <  SRR19973080.368409/150‑1 (MQ=60)

                                                                                                        ATCACAGCTAACAAGTATACCTGGCGTTGTGTGGTTTATTTTTTGGCGGTAAAAAAATTTTATATTTTGTTTTTTGTTATAAAGAAACAAAAGCACCCAAGCCAGACATATAGCCAAAATAAAAATGATAACACcaaaatttttattaag                                              >  SRR19973080.786995/1‑134 (MQ=39)

                                                                                                         TCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAATGATAACACGAATCTTTTTCTTCAGA                                             >  SRR19973080.4107080/1‑150 (MQ=60)

                                                                                                            CAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAA                                          <  SRR19973080.1243657/150‑1 (MQ=60)

                                                                                                            CAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAAAGCCATACATATAGCCCTAATAAAAAGGATAACACGAAACTTTTTCTTCAGATAA                                          >  SRR19973080.2089922/1‑150 (MQ=60)

                                                                                                                  ACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAA                                    <  SRR19973080.2965324/150‑1 (MQ=60)

                                                                                                                      GTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCT                                <  SRR19973080.3695263/150‑1 (MQ=60)

                                                                                                                       TATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGA                                                                                                                       >  SRR19973080.3681180/1‑62 (MQ=0)

                                                                                                                          ACCTGGCGTTGTCTTGTTTATTTTTAGGCTGTAAAaaacatttatattttaattatttataaaaagaaacaaaagcaccaaatccaaacaaaaagccaaaaaaaaaaagaaaacacgaataaatttattaatataaaaaaaaaaataaaa                            >  SRR19973080.206230/1‑35 (MQ=59)

                                                                                                                           CCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACATTTATATTGTATTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAAAACGAATCTTTTTCTTCAGATAAAATAAAATATCAATA                           >  SRR19973080.843636/1‑150 (MQ=60)

                                                                                                                           CCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAAAAAATTTTATATTGTATTTATTGATATACAGAAACAAAAGCACCAAAGCCATACATATAGCCAAAATAAAAAGGATAACACGAATCTTTTTATTCAGATAAAATAAAAaataaatc                           >  SRR19973080.86990/1‑142 (MQ=60)

                                                                                                                            CTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCA                          <  SRR19973080.3716367/150‑1 (MQ=60)

                                                                                                                             TGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTTTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAG                         >  SRR19973080.1972695/1‑150 (MQ=60)

                                                                                                                             TGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAG                         <  SRR19973080.2577165/150‑1 (MQ=60)

                                                                                                                                      CTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATC                <  SRR19973080.4281047/150‑1 (MQ=60)

                                                                                                                                       TGTTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCC               <  SRR19973080.1031684/150‑1 (MQ=60)

                                                                                                                                       TGTTTTATTTTTAGACTGTAAATAACTTTTATATTGTATTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCC               <  SRR19973080.2084519/150‑1 (MQ=60)

                                                                                                                                         GTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTG             >  SRR19973080.1035875/1‑150 (MQ=60)

                                                                                                                                         GTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTG             >  SRR19973080.490341/1‑150 (MQ=60)

                                                                                                                                             ATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTG         >  SRR19973080.3367673/1‑150 (MQ=60)

                                                                                                                                             ATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTG         >  SRR19973080.3366999/1‑150 (MQ=60)

                                                                                                                                               TTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAT       >  SRR19973080.4187785/1‑150 (MQ=60)

                                                                                                                                                 TTAGACTTTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTT     <  SRR19973080.3872626/150‑1 (MQ=60)

                                                                                                                                                 TTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTT     <  SRR19973080.3872929/150‑1 (MQ=60)

                                                                                                                                                   AGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGT   <  SRR19973080.1036902/150‑1 (MQ=60)

                                                                                                                                                    GACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  <  SRR19973080.919742/150‑1 (MQ=60)

CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  >  CP009273/320771‑321068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`