Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 320,920 G→A 100% S52S (AGC→AGT)  ykgH ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273320,9200GA99.7% 855.1 / ‑5.9 253S52S (AGC→AGT) ykgHputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (120/132);  total (120/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 253 total aligned reads displayed.

ATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTT  >  CP009273/320774‑321065
                                                                                                                                                  |                                                                                                                                                 
aTTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACt                                                                                                                                                 <  1:2803784/149‑1 (MQ=255)
  ttCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGt                                                                                                                                               <  2:2401954/149‑1 (MQ=255)
  ttCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGt                                                                                                                                               >  1:3473260/1‑149 (MQ=255)
   tctcTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTa                                                                                                                                              <  1:3875188/149‑1 (MQ=255)
   tctcTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTa                                                                                                                                              >  1:3147389/1‑149 (MQ=255)
    ctctGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTaa                                                                                                                                             <  1:4437420/149‑1 (MQ=255)
     tctGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTaaa                                                                                                                                            >  1:869119/1‑149 (MQ=255)
     tctGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTaaa                                                                                                                                            <  2:317406/149‑1 (MQ=255)
       tGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAAta                                                                                                                                          >  2:2223211/1‑148 (MQ=255)
       tGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATc                                                                                                                                          <  1:4242927/149‑1 (MQ=255)
       tGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATc                                                                                                                                          >  2:2223341/1‑149 (MQ=255)
         ttCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCAc                                                                                                                                        >  1:1110542/1‑149 (MQ=255)
              gACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTta                                                                                                                                   >  2:258789/1‑149 (MQ=255)
               aCATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTtat                                                                                                                                  <  2:1265376/149‑1 (MQ=255)
               aCATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTtat                                                                                                                                  <  2:3774823/149‑1 (MQ=255)
               aCATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTtat                                                                                                                                  <  2:3401871/149‑1 (MQ=255)
                cATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTtata                                                                                                                                 >  1:987717/1‑149 (MQ=255)
                    tGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGt                                                                                                                             >  1:2647782/1‑149 (MQ=255)
                    tGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGt                                                                                                                             >  1:2647878/1‑149 (MQ=255)
                    tGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGt                                                                                                                             <  1:341837/149‑1 (MQ=255)
                     gggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTc                                                                                                                            >  2:3066996/1‑149 (MQ=255)
                      ggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATACTGTAAATCACTTTTATATTGTCt                                                                                                                           >  1:618192/1‑149 (MQ=255)
                       gCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCtt                                                                                                                          <  2:2155500/149‑1 (MQ=255)
                       gCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTGTCtt                                                                                                                          >  1:2009017/1‑149 (MQ=255)
                        cTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCttt                                                                                                                         <  2:2473779/149‑1 (MQ=255)
                        cTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCttt                                                                                                                         <  2:2095767/149‑1 (MQ=255)
                          ggTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTAt                                                                                                                       >  2:674590/1‑149 (MQ=255)
                               gAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGata                                                                                                                  <  1:2104985/149‑1 (MQ=255)
                               gAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGata                                                                                                                  <  1:1808953/149‑1 (MQ=255)
                               gAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGata                                                                                                                  <  2:1418567/149‑1 (MQ=255)
                                  aaaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAc                                                                                                               >  2:4557821/1‑149 (MQ=255)
                                   aaaTGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACa                                                                                                              <  2:3845299/149‑1 (MQ=255)
                                       gtagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGaaa                                                                                                          >  1:3211332/1‑149 (MQ=255)
                                       gtagtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGACAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTTTAAATCACTTTTATATTGTCTTTATTGATATACAGaaa                                                                                                          <  1:3403775/149‑1 (MQ=255)
                                         agtaATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAaaca                                                                                                        >  1:4128006/1‑149 (MQ=255)
                                            aagtCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAacaaca                                                                                                     <  2:4319082/146‑1 (MQ=255)
                                             aTTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAg                                                                                                    >  2:646689/1‑149 (MQ=255)
                                                cGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTCTACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCAc                                                                                                 >  1:2262091/1‑149 (MQ=255)
                                                cGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCAc                                                                                                 >  1:1055784/1‑149 (MQ=255)
                                                cGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGATATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCAc                                                                                                 <  1:4444022/149‑1 (MQ=255)
                                                  cTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCa                                                                                               >  2:911304/1‑149 (MQ=255)
                                                    gAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATa                                                                                             <  1:4400031/149‑1 (MQ=255)
                                                       tAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTTTCTTTATTGATATACAGAAACAACAGCACCATAGcc                                                                                          >  2:3458602/1‑149 (MQ=255)
                                                         aCATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcat                                                                                        >  2:424915/1‑149 (MQ=255)
                                                          cATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGATAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcata                                                                                       <  2:1099494/149‑1 (MQ=255)
                                                            tCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcataca                                                                                     <  2:4167192/149‑1 (MQ=255)
                                                              ttCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcatacata                                                                                   <  1:4784454/149‑1 (MQ=255)
                                                               tCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACatat                                                                                  >  1:2306602/1‑149 (MQ=255)
                                                               tCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACatat                                                                                  <  2:3770045/149‑1 (MQ=255)
                                                                cAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAtata                                                                                 <  2:3565103/149‑1 (MQ=255)
                                                                cAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAtata                                                                                 >  2:3599110/1‑149 (MQ=255)
                                                                cAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACAtata                                                                                 <  2:1712495/149‑1 (MQ=255)
                                                                 aGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAg                                                                                >  1:4376495/1‑149 (MQ=255)
                                                                  gTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGc                                                                               >  1:4675551/1‑149 (MQ=255)
                                                                   tCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTGTTTATTGATATACAGAAAAAACAGCACCATAGCCATACATATAGcc                                                                              >  1:2954109/1‑149 (MQ=255)
                                                                   tCATTGTAGTCTGACAGGCAAAATAATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATAGATATACAGAAACAACAGCACCAAAGCCATACATAAAGcc                                                                              >  2:2888123/1‑149 (MQ=255)
                                                                    cATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCa                                                                             >  1:775636/1‑149 (MQ=255)
                                                                       tGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataa                                                                          <  1:1544268/149‑1 (MQ=255)
                                                                        gTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCataat                                                                         >  2:3077995/1‑149 (MQ=255)
                                                                               gACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAgg                                                                  <  2:3114747/149‑1 (MQ=255)
                                                                                   ggCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTTGAATGTAAATCACTTTTATATTGTGTTTTTTGATATACAGAAAAAAAAGCACCAAATCCATACATATAGCCATAATAAAAAGGATaa                                                              >  1:1271888/1‑149 (MQ=255)
                                                                                      aaaaTTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAacac                                                           <  2:2508153/149‑1 (MQ=255)
                                                                                        aaTTATTAGTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGa                                                         <  2:4521947/149‑1 (MQ=255)
                                                                                         attattattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGaa                                                        >  2:1658145/1‑149 (MQ=255)
                                                                                         attattattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGaa                                                        >  2:4603431/1‑149 (MQ=255)
                                                                                         attattattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGaa                                                        >  2:1757678/1‑149 (MQ=255)
                                                                                              tattGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttt                                                   <  1:1923747/149‑1 (MQ=255)
                                                                                               attGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCtttt                                                  <  1:1019063/149‑1 (MQ=255)
                                                                                               attGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCtttt                                                  <  1:1766632/149‑1 (MQ=255)
                                                                                               attGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCtttt                                                  <  1:3073076/149‑1 (MQ=255)
                                                                                                ttGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCttttt                                                 <  1:608250/149‑1 (MQ=255)
                                                                                                  gCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTTTACTGTAAATAACTTTTATATTGTCGTTATTGATATACAGAAAAAACAGCACCAAAGCCATACATATATCCAAAATAAAAATGATAACAAGAATCTTTttct                                               >  1:4015129/1‑149 (MQ=255)
                                                                                                    tATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTttcttc                                             <  1:1691289/149‑1 (MQ=255)
                                                                                                     aTCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCa                                            <  2:2318294/149‑1 (MQ=255)
                                                                                                        acaGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGat                                         <  2:2609250/149‑1 (MQ=255)
                                                                                                            cTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaa                                     >  1:2336226/1‑149 (MQ=255)
                                                                                                             tAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaat                                    <  2:2924323/149‑1 (MQ=255)
                                                                                                             tAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaat                                    <  2:511249/149‑1 (MQ=255)
                                                                                                              aaCAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGataaaata                                   <  2:4386707/149‑1 (MQ=255)
                                                                                                              aaCAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAAAGCCATACATATAGCCATAATAAAAATGATAACACGAATATTTTTATTCAGataaaata                                   >  1:142721/1‑149 (MQ=255)
                                                                                                                  aGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATaaaataaaat                               >  2:606329/1‑149 (MQ=255)
                                                                                                                   gTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAAtc                              <  2:1592229/149‑1 (MQ=255)
                                                                                                                      taCCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCa                           <  1:1434025/149‑1 (MQ=255)
                                                                                                                       aCCTGGCGTTGTCTGGTTTATTTTTATACTGTAAATCACATTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATATCCATACATATAGCCAAAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTaaa                          >  1:4707410/1‑149 (MQ=255)
                                                                                                                         cTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATc                        <  2:2078311/149‑1 (MQ=255)
                                                                                                                         cTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATc                        <  2:4476113/149‑1 (MQ=255)
                                                                                                                          tGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCa                       >  1:1329776/1‑149 (MQ=255)
                                                                                                                          tGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCa                       >  1:1396451/1‑149 (MQ=255)
                                                                                                                          tGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCa                       >  1:3718435/1‑149 (MQ=255)
                                                                                                                              gTTGTCTGGTTTATTTTTATACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATc                   <  1:2986330/149‑1 (MQ=255)
                                                                                                                                  tCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGata               <  1:3577670/149‑1 (MQ=255)
                                                                                                                                    tGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATc             >  1:4625379/1‑149 (MQ=255)
                                                                                                                                     ggTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATcc            <  2:1570338/149‑1 (MQ=255)
                                                                                                                                         tATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGtt        <  2:1083803/149‑1 (MQ=255)
                                                                                                                                         tATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGtt        >  1:386613/1‑149 (MQ=255)
                                                                                                                                         tATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGtt        >  1:1783624/1‑149 (MQ=255)
                                                                                                                                          aTTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGttt       >  1:101139/1‑149 (MQ=255)
                                                                                                                                          aTTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGttt       >  1:1179258/1‑149 (MQ=255)
                                                                                                                                              ttAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAtt   <  1:4078701/149‑1 (MQ=255)
                                                                                                                                               tAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGAttt  >  2:2486563/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTT  >  CP009273/320774‑321065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  >  CP009273/320771‑321068
                                                                                                                                                     |                                                                                                                                                    
CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGA                                                                                                                                                      >  SRR19973071.3672414/1‑150 (MQ=60)
  TATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACT                                                                                                                                                    <  SRR19973071.3782618/150‑1 (MQ=60)
  TATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACT                                                                                                                                                    <  SRR19973071.4127884/150‑1 (MQ=60)
  TAGTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACT                                                                                                                                                    <  SRR19973071.3782740/150‑1 (MQ=60)
   ATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTG                                                                                                                                                   >  SRR19973071.2811973/1‑150 (MQ=60)
   ATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTG                                                                                                                                                   <  SRR19973071.2813644/150‑1 (MQ=60)
   ATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTG                                                                                                                                                   >  SRR19973071.4755515/1‑150 (MQ=60)
    TTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGT                                                                                                                                                  >  SRR19973071.3485498/1‑150 (MQ=60)
    TTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGT                                                                                                                                                  >  SRR19973071.617774/1‑150 (MQ=60)
     TTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATACTGTA                                                                                                                                                 >  SRR19973071.4411547/1‑150 (MQ=60)
     TTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTA                                                                                                                                                 >  SRR19973071.3158411/1‑150 (MQ=60)
      TCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAA                                                                                                                                                <  SRR19973071.3888644/150‑1 (MQ=60)
      TCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAA                                                                                                                                                <  SRR19973071.3888893/150‑1 (MQ=60)
       CTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAA                                                                                                                                               <  SRR19973071.4453090/150‑1 (MQ=60)
       CTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAA                                                                                                                                               >  SRR19973071.872228/1‑150 (MQ=60)
       CTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAA                                                                                                                                               <  SRR19973071.4453120/150‑1 (MQ=60)
         CTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATC                                                                                                                                             <  SRR19973071.126293/150‑1 (MQ=60)
          TGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCA                                                                                                                                            <  SRR19973071.4258001/150‑1 (MQ=60)
          TGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCA                                                                                                                                            <  SRR19973071.2984844/150‑1 (MQ=60)
          TGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCA                                                                                                                                            <  SRR19973071.2984540/150‑1 (MQ=60)
          TGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCA                                                                                                                                            >  SRR19973071.978581/1‑150 (MQ=60)
          TGTTCCTGACATCTGGGCTGGTGAGAAAAAATGTAGTAAGTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCA                                                                                                                                            <  SRR19973071.4257715/150‑1 (MQ=60)
           GTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCAC                                                                                                                                           >  SRR19973071.1114452/1‑150 (MQ=60)
                TGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTA                                                                                                                                      >  SRR19973071.1421003/1‑150 (MQ=60)
                  ACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATA                                                                                                                                    >  SRR19973071.991309/1‑150 (MQ=60)
                      CTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGT                                                                                                                                >  SRR19973071.2657299/1‑150 (MQ=60)
                      CTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGT                                                                                                                                >  SRR19973071.2657395/1‑150 (MQ=60)
                      CTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGT                                                                                                                                >  SRR19973071.2657344/1‑150 (MQ=60)
                       TGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTC                                                                                                                               <  SRR19973071.342969/150‑1 (MQ=60)
                       TGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTC                                                                                                                               <  SRR19973071.4279208/150‑1 (MQ=60)
                       TGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTC                                                                                                                               <  SRR19973071.4279221/150‑1 (MQ=60)
                        GGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATAATGTAAATCACTTTTATATTGTCT                                                                                                                              >  SRR19973071.620478/1‑150 (MQ=60)
                         GGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTT                                                                                                                             <  SRR19973071.1808111/150‑1 (MQ=60)
                         GGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTT                                                                                                                             <  SRR19973071.1808096/150‑1 (MQ=60)
                         GGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTGTCTT                                                                                                                             >  SRR19973071.2016278/1‑150 (MQ=60)
                         GGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACATTTATATTttatt                                                                                                                             >  SRR19973071.2016580/1‑145 (MQ=60)
                           CTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTA                                                                                                                           >  SRR19973071.3466659/1‑150 (MQ=60)
                             GGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATT                                                                                                                         <  SRR19973071.3793651/150‑1 (MQ=60)
                             GGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATT                                                                                                                         >  SRR19973071.3138546/1‑150 (MQ=60)
                             GGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTATGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATT                                                                                                                         >  SRR19973071.3977447/1‑150 (MQ=60)
                                  GAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATAT                                                                                                                    <  SRR19973071.2112018/150‑1 (MQ=60)
                                  GAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATAT                                                                                                                    <  SRR19973071.2112750/150‑1 (MQ=60)
                                  GAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATAT                                                                                                                    <  SRR19973071.2112786/150‑1 (MQ=60)
                                  GAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATAT                                                                                                                    <  SRR19973071.4690106/150‑1 (MQ=60)
                                  GAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATAT                                                                                                                    <  SRR19973071.1815696/150‑1 (MQ=60)
                                    AAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTATGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATAC                                                                                                                  >  SRR19973071.170435/1‑150 (MQ=60)
                                     AAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACA                                                                                                                 <  SRR19973071.2301444/150‑1 (MQ=60)
                                     AAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACA                                                                                                                 <  SRR19973071.1487313/150‑1 (MQ=60)
                                         TGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTTACTGGTTTATTTTTATACTGTAAATAAATTTTATATTGTCTTTATTGATATACAAAAA                                                                                                             >  SRR19973071.1394139/1‑150 (MQ=60)
                                         TGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAA                                                                                                             >  SRR19973071.3222583/1‑150 (MQ=60)
                                          GTAGTAATTCGCTGAATAACATCTTCAGTGTTTGTAGTCTGACAGACAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTTTAAATCACTTTTATATTGTCTTTATTGATATACAGAAAC                                                                                                            <  SRR19973071.3415829/150‑1 (MQ=60)
                                          GTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGACAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAAC                                                                                                            <  SRR19973071.3415918/150‑1 (MQ=60)
                                          GTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGACAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAAC                                                                                                            <  SRR19973071.3415827/150‑1 (MQ=60)
                                          GTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGACAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAAC                                                                                                            <  SRR19973071.1020098/150‑1 (MQ=60)
                                           TAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACA                                                                                                           >  SRR19973071.4142701/1‑150 (MQ=60)
                                                  TCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTCTACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCAC                                                                                                    >  SRR19973071.2270421/1‑150 (MQ=60)
                                                  TCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCAC                                                                                                    >  SRR19973071.1059052/1‑150 (MQ=60)
                                                  TCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCAC                                                                                                    >  SRR19973071.1059567/1‑150 (MQ=60)
                                                  TCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCAC                                                                                                    >  SRR19973071.1059568/1‑150 (MQ=60)
                                                   CGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGATATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACC                                                                                                   <  SRR19973071.4459725/150‑1 (MQ=60)
                                                     CTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATAATGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAAAAACAGCACCAT                                                                                                 >  SRR19973071.2945674/1‑150 (MQ=60)
                                                       GAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAG                                                                                               <  SRR19973071.4415482/150‑1 (MQ=60)
                                                       GAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAG                                                                                               >  SRR19973071.3177050/1‑150 (MQ=60)
                                                        AATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGC                                                                                              >  SRR19973071.1149099/1‑150 (MQ=60)
                                                        AATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGC                                                                                              >  SRR19973071.1763090/1‑150 (MQ=60)
                                                             CATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATAC                                                                                         >  SRR19973071.3119233/1‑150 (MQ=60)
                                                             CATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATAC                                                                                         <  SRR19973071.3463161/150‑1 (MQ=60)
                                                             CATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATAC                                                                                         <  SRR19973071.1448728/150‑1 (MQ=60)
                                                                CTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATA                                                                                      <  SRR19973071.2297017/150‑1 (MQ=60)
                                                                 TTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATAT                                                                                     >  SRR19973071.2315021/1‑150 (MQ=60)
                                                                 TTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATAT                                                                                     <  SRR19973071.4801319/150‑1 (MQ=60)
                                                                 TTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATAT                                                                                     >  SRR19973071.2315058/1‑150 (MQ=60)
                                                                   CAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAG                                                                                   >  SRR19973071.4391870/1‑150 (MQ=60)
                                                                   CAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAG                                                                                   >  SRR19973071.1994453/1‑150 (MQ=60)
                                                                    AGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGC                                                                                  >  SRR19973071.4692190/1‑150 (MQ=60)
                                                                     GTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTGTTTATTGATATACAGAAAAAACAGCACCATAGCCATACATATAGCC                                                                                 >  SRR19973071.2964691/1‑150 (MQ=60)
                                                                      TCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCA                                                                                >  SRR19973071.778248/1‑150 (MQ=60)
                                                                          TGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAAT                                                                            <  SRR19973071.1549794/150‑1 (MQ=60)
                                                                             AGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAA                                                                         >  SRR19973071.728002/1‑150 (MQ=60)
                                                                              GTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAA                                                                        <  SRR19973071.1434822/150‑1 (MQ=60)
                                                                              GTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAA                                                                        <  SRR19973071.3643759/150‑1 (MQ=60)
                                                                                     AGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTTGAATGTAAATCACTTTTATATTGTGTTTTTTGATATACAGAAAAAAAAGCACCAAATCCATACATATAGCCATAATAAAAAGGATAA                                                                 >  SRR19973071.1276489/1‑150 (MQ=60)
                                                                                      GGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAAC                                                                <  SRR19973071.1999180/150‑1 (MQ=60)
                                                                                        CAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACAC                                                              <  SRR19973071.3754167/150‑1 (MQ=60)
                                                                                              TATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCT                                                        <  SRR19973071.3524256/150‑1 (MQ=60)
                                                                                               ATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTT                                                       <  SRR19973071.775643/150‑1 (MQ=60)
                                                                                                 TATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTT                                                     <  SRR19973071.1930775/150‑1 (MQ=60)
                                                                                                  ATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTT                                                    <  SRR19973071.1022758/150‑1 (MQ=60)
                                                                                                  ATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTT                                                    <  SRR19973071.1773228/150‑1 (MQ=60)
                                                                                                  ATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTT                                                    <  SRR19973071.3083959/150‑1 (MQ=60)
                                                                                                  ATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCAAAATAAAAAGGATAACACGAATCTTTTT                                                    >  SRR19973071.3251290/1‑150 (MQ=60)
                                                                                                   TTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTC                                                   <  SRR19973071.610501/150‑1 (MQ=60)
                                                                                                    TGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTTTACTGTAAATAACTTTTATATTGTCGTTATTGATATACAGAAAAAACAGCACCAAAGCCATACATATATCCAAAATAAAAATGATAACAAGAATCTTTTTCT                                                  >  SRR19973071.4029230/1‑150 (MQ=60)
                                                                                                    TGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCCTACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCT                                                  >  SRR19973071.1775171/1‑150 (MQ=60)
                                                                                                    TGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACATTTATATTGTCTTTATTGCTATACAGAAACAACAGCACCAAAGCCAAACAAATAGCCAAAATAAAAAGGATAAAACGAAaatttttat                                                  >  SRR19973071.4029125/1‑141 (MQ=60)
                                                                                                    TGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAAAAACATTTATATTGTCTTTATTGATATAAAGAAACAACAGCACCAAAGCCAAAAAAATAGCCATAATAAAAATGATAAAACGAAACATTTTCT                                                  >  SRR19973071.4030149/1‑150 (MQ=60)
                                                                                                       TATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCA                                               <  SRR19973071.4123819/150‑1 (MQ=60)
                                                                                                       TATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCA                                               <  SRR19973071.1282653/150‑1 (MQ=60)
                                                                                                       TATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCA                                               <  SRR19973071.1697532/150‑1 (MQ=60)
                                                                                                        ATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGACTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAG                                              <  SRR19973071.409307/150‑1 (MQ=60)
                                                                                                         TCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCCCATTTATATTTTGTTTATTGATATACAGAAACAACAGCACCCAAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTAGTCAGA                                             >  SRR19973071.112162/1‑150 (MQ=60)
                                                                                                             AGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTATACTGTAAATAACTTTTATATTTTGTTTATTTATATAAAGAAACAAAATCACCATAGCCAAAAAAATATCCATAATAAAAAGGATAAaaagaaaatttttattaagataaa                                         >  SRR19973071.1647462/1‑126 (MQ=60)
                                                                                                              GCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAA                                        >  SRR19973071.2344714/1‑150 (MQ=60)
                                                                                                               CTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAAT                                       >  SRR19973071.4715096/1‑150 (MQ=60)
                                                                                                                TAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCAAAGCCATACATATAGCCATAATAAAAATGATAACACGAATATTTTTATTCAGATAAAATA                                      >  SRR19973071.143387/1‑150 (MQ=60)
                                                                                                                    AAGTATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACATTTATATTTTCTTTATTTATAAACAGAAAAAACAGCACCAAATCCAAACAAATAGACAAAATAAAAATGATAACACAAATATTTTTATTAAGATAAAAAAAAAT                                  >  SRR19973071.545105/1‑150 (MQ=53)
                                                                                                                       TATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTC                               <  SRR19973071.452366/150‑1 (MQ=60)
                                                                                                                       TATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTGTCTTTATTGATATAAAGAAACAACAGCACCAAAGCCAAACATATAGCCATAATAAAAAGGATAACACGAATATTTTTATTCAGAAAAAATAAAATCTC                               >  SRR19973071.1044882/1‑150 (MQ=60)
                                                                                                                        ATACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACATTTATATTGTCTTTATTGATATAAAGAAACAACAGCACCAAAGCCATACAAATATCCATAATAAAAATGATAACACGAAACTTTTTCTTCAGATAAAATAAAATCaaa                              >  SRR19973071.3027456/1‑147 (MQ=60)
                                                                                                                         TACCTGGCGTTGTCTGGTTTATTTTTATACTGTAAATCACATTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATATCCATACATATAGCCAAAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTAAA                             >  SRR19973071.4724114/1‑150 (MQ=60)
                                                                                                                         TACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAA                             <  SRR19973071.1439292/150‑1 (MQ=60)
                                                                                                                         TACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAA                             <  SRR19973071.1865764/150‑1 (MQ=60)
                                                                                                                         TACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATAACTTTTATATTGTCTTTATTTATATAAAGAAAAAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTATTAAGATAAAATAAAATATCAA                             >  SRR19973071.4724061/1‑150 (MQ=60)
                                                                                                                          ACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAAT                            <  SRR19973071.1281377/150‑1 (MQ=60)
                                                                                                                          ACCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAAT                            <  SRR19973071.860759/150‑1 (MQ=60)
                                                                                                                           CCTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATC                           <  SRR19973071.4444844/150‑1 (MQ=60)
                                                                                                                            CTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCA                          >  SRR19973071.1334659/1‑150 (MQ=60)
                                                                                                                            CTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCA                          <  SRR19973071.3688242/150‑1 (MQ=60)
                                                                                                                            CTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCA                          >  SRR19973071.3731609/1‑150 (MQ=60)
                                                                                                                            CTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCA                          >  SRR19973071.1401613/1‑150 (MQ=60)
                                                                                                                            CTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCA                          >  SRR19973071.1401596/1‑150 (MQ=60)
                                                                                                                            CTGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCA                          >  SRR19973071.4427552/1‑150 (MQ=60)
                                                                                                                             TGGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAG                         <  SRR19973071.2440901/150‑1 (MQ=60)
                                                                                                                              GGCGTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGACTCTTTTTCTTCAGATAAAATAAAATCTCAATCAGA                        >  SRR19973071.2695644/1‑150 (MQ=60)
                                                                                                                                 GTTGTCTGGTTTATTTTTATACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCG                     <  SRR19973071.2997015/150‑1 (MQ=60)
                                                                                                                                 GTTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCG                     <  SRR19973071.513185/150‑1 (MQ=60)
                                                                                                                                  TTGTCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGA                    <  SRR19973071.832460/150‑1 (MQ=60)
                                                                                                                                     TCTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATAT                 <  SRR19973071.3590106/150‑1 (MQ=60)
                                                                                                                                      CTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATC                >  SRR19973071.4641866/1‑150 (MQ=60)
                                                                                                                                      CTGGTTTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATC                >  SRR19973071.4641736/1‑150 (MQ=60)
                                                                                                                                           TTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTT           >  SRR19973071.1790274/1‑150 (MQ=60)
                                                                                                                                           TTATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTT           >  SRR19973071.387821/1‑150 (MQ=60)
                                                                                                                                            TATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTT          >  SRR19973071.1183318/1‑150 (MQ=60)
                                                                                                                                            TATTTTTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTT          >  SRR19973071.101658/1‑150 (MQ=60)
                                                                                                                                                 TTAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTT     <  SRR19973071.4093171/150‑1 (MQ=60)
                                                                                                                                                  TAGACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTG    <  SRR19973071.2507253/150‑1 (MQ=60)
                                                                                                                                                    GACTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  >  SRR19973071.1026454/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTT  >  CP009273/320771‑321068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑